Coexistence of Helicobacter pylori spiral and coccoid forms in experimental mice.

Abstract:

:AIM:To infect mice with Helicobacter pylori and detect immune response against two form of H. pylori.METHODS:An isolate of H. pylori obtained from a patient with gastric cancer was used to infect mice. Fifty mice were divided into eight groups. Two groups served as negative control without any inoculation and internal negative control with 0.5M NaHCO(3) and brain heart infusion (HBI), respectively. Mice in each experimental group were first inoculated with 0.5M NaHCO(3) and then H. pylori suspension for 3 times at a 2-day interval. Mice from controls and infectious groups were sacrificed at a weekly interval postinfection. Gastric samples were trimmed, inoculated onto chocolate blood agar and then incujbated in microaerophilic atmosphere at 37° for 14 days. Sera were examined for immunoglobulins against H. pylori spiral and coccoid antigens by ELISA.RESULTS:After inoculation H. pylori was isolated in one mouse from one week postinfection.No H. pylori was detected in control mice. However,urease test was positive in 50% (5/10) control mice, 70% (7/10) mice inoculated with NaHCO(3) and BHI and 77% (23/30) mice infected with H. pylori. The systemic immune responses of the mice to H. pylori strain were determined by ELISA. The mice showed immune responses to both H. pylori spiral and coccoid antigens one week after infection with H. pylori. The peak mean absorbances of antibodies against spiral and coccoid forms were four weeks postinfection which showed 6 and 18 times higher than that of negative control group respectively (P < 0.01).CONCLUSION:Spiral and coccoid forms of H. pylori coexist in experimental mice studied.

journal_name

World J Gastroenterol

authors

Hua JS,Bow H,Zheng PY,Yeoh GK,Ng CH,Lim GS

doi

10.3748/wjg.v4.i6.485

keywords:

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

485-488

issue

6

eissn

1007-9327

issn

2219-2840

journal_volume

4

pub_type

杂志文章
  • Targeted and immune therapies for hepatocellular carcinoma: Predictions for 2019 and beyond.

    abstract::Systemic therapy for hepatocellular carcinoma (HCC) has markedly advanced since the survival benefit of a molecular targeted agent, sorafenib, were demonstrated in the SHARP and Asia Pacific trials in 2007. Treatment options for patients with advanced HCC increased by sorafenib, and long-term survival for patients wit...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v25.i7.789

    authors: Kudo M

    更新日期:2019-02-21 00:00:00

  • Expression of E-selectin, integrin beta1 and immunoglobulin superfamily member in human gastric carcinoma cells and its clinicopathologic significance.

    abstract:AIM:To study the expression levels of E- selectin, integrin beta1 and immunoglobulin superfamily member-intercellular adhesion molecule-1 (ICAM-1) in human gastric carcinoma cells, and to explore the relationship between these three kinds of cell adhesion molecules and gastric carcinoma. METHODS:The serum contents of ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i22.3609

    authors: Ke JJ,Shao QS,Ling ZQ

    更新日期:2006-06-14 00:00:00

  • Diagnostic accuracy of tests for Helicobacter pylori in an Alaska Native population.

    abstract:AIM:To evaluate the accuracy of two non-invasive tests in a population of Alaska Native persons. High rates of Helicobacter pylori (H. pylori) infection, H. pylori treatment failure, and gastric cancer in this population necessitate documentation of infection status at multiple time points over a patient's life. METHO...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v17.i42.4682

    authors: Bruden DL,Bruce MG,Miernyk KM,Morris J,Hurlburt D,Hennessy TW,Peters H,Sacco F,Parkinson AJ,McMahon BJ

    更新日期:2011-11-14 00:00:00

  • Gastrointestinal stromal tumors of the duodenum: surgical management and survival results.

    abstract:AIM:To provide long-term survival results of operable duodenal gastrointestinal stromal tumors (DGISTs) in a tertiary center in China. METHODS:In this retrospective study, the pathological data of 28 patients with DGISTs who had been treated surgically at the Second Department of General Surgery, Sir Run Run Shaw Hosp...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v19.i36.6000

    authors: Liang X,Yu H,Zhu LH,Wang XF,Cai XJ

    更新日期:2013-09-28 00:00:00

  • Elastography-based screening for esophageal varices in patients with advanced chronic liver disease.

    abstract::Elastography-based liver stiffness measurement (LSM) is a non-invasive tool for estimating liver fibrosis but also provides an estimate for the severity of portal hypertension in patients with advanced chronic liver disease (ACLD). The presence of varices and especially of varices needing treatment (VNT) indicates dis...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v25.i3.308

    authors: Paternostro R,Reiberger T,Bucsics T

    更新日期:2019-01-21 00:00:00

  • Suspicious brush cytology is an indication for liver transplantation evaluation in primary sclerosing cholangitis.

    abstract:AIM:To investigate markers for high-grade dysplasia for the optimal timing of liver transplantation in patients with primary sclerosing cholangitis (PSC). METHODS:Earlier data support a dysplasia-carcinoma sequence, even low- to high-grade dysplasia, in PSC-associated cholangiocarcinoma (CCA). Surveillance using endos...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v23.i33.6147

    authors: Boyd S,Vannas M,Jokelainen K,Isoniemi H,Mäkisalo H,Färkkilä MA,Arola J

    更新日期:2017-09-07 00:00:00

  • Differences in viral kinetics between genotypes 1 and 3 of hepatitis C virus and between cirrhotic and non-cirrhotic patients during antiviral therapy.

    abstract:AIM:To evaluate the impact of hepatitis C virus (HCV) infection with genotype 1 or 3 and the presence or absence of liver cirrhosis (LC) in the early viral kinetics response to treatment. METHODS:Naive patients (n = 46) treated with interferon-alpha (IFN-alpha) and ribavirin and followed up with frequent early HCV-RNA...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i45.7271

    authors: Medeiros-Filho JE,de Carvalho Mello IM,Pinho JR,Neumann AU,de Mello Malta F,da Silva LC,Carrilho FJ

    更新日期:2006-12-07 00:00:00

  • Subtotal colonic bypass plus colostomy with antiperistaltic cecoproctostomy for the treatment of slow transit constipation in an aged population: A retrospective control study.

    abstract:AIM:To compare the efficacy, improved quality of life, and prognosis in patients undergoing either subtotal colonic bypass with antiperistaltic cecoproctostomy (SCBAC) or subtotal colonic bypass plus colostomy with antiperistaltic cecoproctostomy (SCBCAC) for the treatment of slow transit constipation. METHODS:Between...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v24.i23.2491

    authors: Yang Y,Cao YL,Wang WH,Zhang YY,Zhao N,Wei D

    更新日期:2018-06-21 00:00:00

  • Simultaneous transcatheter arterial chemoembolization and portal vein embolization for patients with large hepatocellular carcinoma before major hepatectomy.

    abstract:BACKGROUND:Sequential transarterial chemoembolization (TACE) and portal vein embolization (PVE) are associated with long time interval that can allow tumor growth and nullify treatments' benefits. AIM:To evaluate the effect of simultaneous TACE and PVE for patients with large hepatocellular carcinoma (HCC) prior to el...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v26.i30.4489

    authors: Zhang CW,Dou CW,Zhang XL,Liu XQ,Huang DS,Hu ZM,Liu J

    更新日期:2020-08-14 00:00:00

  • Characteristics of upper digestive tract diseases in Bohai Bay fishermen.

    abstract:AIM:To study the characteristics of upper digestive tract diseases (UDTDs) in fishermen who live in Bohai Bay. METHODS:An investigation was carried out in 1488 fishermen with symptoms of UDTDs (aside from liver, biliary and pancreatic diseases) during the time period between December 1991 and February 1995. This inves...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v3.i3.171

    authors: Wang YB,Wang YP,Zou J,Bai BJ,Ren GC,Cai BQ

    更新日期:1997-09-15 00:00:00

  • Acute pancreatitis: the stress factor.

    abstract::Acute pancreatitis is an inflammatory disorder of the pancreas that may cause life-threatening complications. Etiologies of pancreatitis vary, with gallstones accounting for the majority of all cases, followed by alcohol. Other causes of pancreatitis include trauma, ischemia, mechanical obstruction, infections, autoim...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i19.5801

    authors: Binker MG,Cosen-Binker LI

    更新日期:2014-05-21 00:00:00

  • Management of antiviral drug resistance in chronic hepatitis B.

    abstract::Rescue antiviral treatment for patients with resistance to preexisting nucleos(t)ide analogues remains a clinical challenge. The correct choice of a first-line treatment of high potency and with a high genetic barrier to achieve sustained long-term suppression of viral replication provides the best chance of preventin...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i33.11641

    authors: Bang KB,Kim HJ

    更新日期:2014-09-07 00:00:00

  • Pharmacopoeia of acute pancreatitis: is the roster complete?

    abstract::Acute pancreatitis is one of the most common diseases in the everyday's practise of gastroenterologists and surgeons. However, the physicians' therapeutic armamentarium is very limited. The present letter to the editor briefly describes the recent evidence from the literature with the aim to optimize a conservative ma...

    journal_title:World journal of gastroenterology

    pub_type: 评论,信件

    doi:10.3748/wjg.14.5938

    authors: Petrov MS

    更新日期:2008-10-14 00:00:00

  • Application of robotics in gastrointestinal endoscopy: A review.

    abstract::Multiple robotic flexible endoscope platforms have been developed based on cross specialty collaboration between engineers and medical doctors. However, significant number of these platforms have been developed for the natural orifice transluminal endoscopic surgery paradigm. Increasing amount of evidence suggest the ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v22.i5.1811

    authors: Yeung BP,Chiu PW

    更新日期:2016-02-07 00:00:00

  • Prognostic value of sorting nexin 10 weak expression in stomach adenocarcinoma revealed by weighted gene co-expression network analysis.

    abstract:AIM:To detect significant clusters of co-expressed genes associated with tumorigenesis that might help to predict stomach adenocarcinoma (SA) prognosis. METHODS:The Cancer Genome Atlas database was used to obtain RNA sequences as well as complete clinical data of SA and adjacent normal tissues from patients. Weighted ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v24.i43.4906

    authors: Zhang J,Wu Y,Jin HY,Guo S,Dong Z,Zheng ZC,Wang Y,Zhao Y

    更新日期:2018-11-21 00:00:00

  • Honey prevents hepatic damage induced by obstruction of the common bile duct.

    abstract:AIM:To examine the possible effects of honey supplementation on hepatic damage due to obstruction of the common bile duct in an experimental rat model. METHODS:The study was performed with 30 male rats divided into three groups: a sham group, an obstructive jaundice group, and an obstructive jaundice plus honey group....

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.14.3729

    authors: Erguder BI,Kilicoglu SS,Namuslu M,Kilicoglu B,Devrim E,Kismet K,Durak I

    更新日期:2008-06-21 00:00:00

  • Autoimmune hepatitis, one disease with many faces: etiopathogenetic, clinico-laboratory and histological characteristics.

    abstract::Autoimmune hepatitis (AIH) is an unresolving progressive liver disease of unknown etiology characterized by hypergammaglobulinemia, autoantibodies detection and interface hepatitis. Due to the absence of specific diagnostic markers and the large heterogeneity of its clinical, laboratory and histological features, AIH ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v21.i1.60

    authors: Gatselis NK,Zachou K,Koukoulis GK,Dalekos GN

    更新日期:2015-01-07 00:00:00

  • Effect of interleukin-10 and platelet-derived growth factor on expressions of matrix metalloproteinases-2 and tissue inhibitor of metalloproteinases-1 in rat fibrotic liver and cultured hepatic stellate cells.

    abstract:AIM:To examine the expressions of matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in rat fibrotic liver and in normal rat hepatic stellate cells, and to investigate the changes in their expressions in response to treatment with interleukin-10 (IL-10) and platelet-derived growth...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v10.i17.2574

    authors: Zhang LJ,Chen YX,Chen ZX,Huang YH,Yu JP,Wang XZ

    更新日期:2004-09-01 00:00:00

  • Glycyrrhizic acid inhibits apoptosis and fibrosis in carbon-tetrachloride-induced rat liver injury.

    abstract:AIM:To investigate anti-apoptotic effects of glycyrrhizic acid (GA) against fibrosis in carbon tetrachloride (CCl4)-induced liver injury and its contributing factors. METHODS:Liver fibrosis was induced by administration of CCl4 for 8 wk. Pathological changes in the liver of rats were examined by hematoxylin-eosin stai...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v21.i17.5271

    authors: Liang B,Guo XL,Jin J,Ma YC,Feng ZQ

    更新日期:2015-05-07 00:00:00

  • Efficacy of noninvasive evaluations in monitoring inflammatory bowel disease activity: A prospective study in China.

    abstract:AIM:To optimize the efficacy of noninvasive evaluations in monitoring the endoscopic activity of inflammatory bowel disease (IBD). METHODS:Fecal calprotectin (FC), clinical activity index (CDAI or CAI), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and procalcitonin (PCT) were measured for 136 IBD pa...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v23.i46.8235

    authors: Chen JM,Liu T,Gao S,Tong XD,Deng FH,Nie B

    更新日期:2017-12-14 00:00:00

  • Expression of c-fos in gastric myenteric plexus and spinal cord of rats with cervical spondylosis.

    abstract:AIM:To determine the expression of c-fos in gastric myenteric plexus and spinal cord of rats with cervical spondylosis and its clinical significance. METHODS:A cervical spondylosis model was established in rats by destroying the stability of cervical posterior column, and the cord segments C(4-6) and gastric antrum we...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v11.i4.529

    authors: Song PS,Kong KM,Niu CY,Qi WL,Wu LF,Wang XJ,Han W,Huang K,Chen ZF

    更新日期:2005-01-28 00:00:00

  • Prognostic factors in patients with advanced cholangiocarcinoma: role of surgery, chemotherapy and body mass index.

    abstract:AIM:To study the factors that may affect survival of cholangiocarcinoma in Lebanon. METHODS:A retrospective review of the medical records of 55 patients diagnosed with cholangio-carcinoma at the American University of Beirut between 1990 and 2005 was conducted. Univariate and multivariate analyses were performed to de...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.14.3224

    authors: Farhat MH,Shamseddine AI,Tawil AN,Berjawi G,Sidani C,Shamseddeen W,Barada KA

    更新日期:2008-05-28 00:00:00

  • Effect of rifaximin on gut microbiota composition in advanced liver disease and its complications.

    abstract::Liver cirrhosis is a paradigm of intestinal dysbiosis. The qualitative and quantitative derangement of intestinal microbial community reported in cirrhotic patients seems to be strictly related with the impairment of liver function. A kind of gut microbial "fingerprint", characterized by the reduced ratio of "good" to...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v21.i43.12322

    authors: Ponziani FR,Gerardi V,Pecere S,D'Aversa F,Lopetuso L,Zocco MA,Pompili M,Gasbarrini A

    更新日期:2015-11-21 00:00:00

  • Pancreatic cancer: advances in treatment.

    abstract::Pancreatic cancer is a leading cause of cancer mortality and the incidence of this disease is expected to continue increasing. While patients with pancreatic cancer have traditionally faced a dismal prognosis, over the past several years various advances in diagnosis and treatment have begun to positively impact this ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i28.9354

    authors: Mohammed S,Van Buren G 2nd,Fisher WE

    更新日期:2014-07-28 00:00:00

  • G and D cells in rat antral mucosa: an immunoelectron microscopic study.

    abstract:AIM:To investigate the gastrin secreting cells (G cells) and the somatostatin secreting cells (D cells) of antral mucosa in rats at the ultrastructural level. METHODS:Revised immunoelectron microscopic technique was used to detect the G cells and D cells in rat antral mucosa through gastrin and somatostatin antibodies...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v9.i12.2768

    authors: Sun FP,Song YG

    更新日期:2003-12-01 00:00:00

  • Liver as a target of human immunodeficiency virus infection.

    abstract::Liver injury is a characteristic feature of human immunodeficiency virus (HIV) infection, which is the second most common cause of mortality in HIV-infected patients. Now it is recognized that liver plays a key role in HIV infection pathogenesis. Antiretroviral therapy (ART), which suppresses HIV infection in permissi...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v24.i42.4728

    authors: Ganesan M,Poluektova LY,Kharbanda KK,Osna NA

    更新日期:2018-11-14 00:00:00

  • Short-term effectiveness of radiochemoembolization for selected hepatic metastases with a combination protocol.

    abstract:AIM:To introduce the combination method of radiochemoembolization for the treatment of selected hepatic metastases. METHODS:Twenty patients with biopsy proven hepatic metastases were selected from those who underwent transarterial radiochemoembolization, a novel combination protocol, between January 2009 and July 2010...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v18.i37.5249

    authors: Akhlaghpoor S,Aziz-Ahari A,Amoui M,Tolooee S,Poorbeigi H,Sheybani S

    更新日期:2012-10-07 00:00:00

  • Measurement of biological age may help to assess the risk of colorectal adenoma in screening colonoscopy.

    abstract:AIM:To investigate the clinical utility of biological age (BA) measurement in screening colonoscopy for the detection of colorectal adenomas in the average-risk population. METHODS:A consecutive series of asymptomatic subjects aged ≥ 30 years who underwent colonoscopy in routine check-ups were enrolled. Colorectal ade...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v23.i37.6877

    authors: Kim SJ,Kim BJ,Kang H

    更新日期:2017-10-07 00:00:00

  • Stronger inhibition of gastric acid secretion by lafutidine, a novel H2 receptor antagonist, than by the proton pump inhibitor lansoprazole.

    abstract:AIM:To compare the antisecretory activity and plasma drug concentrations of a single oral dose of 10 mg lafutidine, a novel H2 receptor antagonist, with those of the proton pump inhibitor lansoprazole (LPZ) 30 mg. METHODS:Ten volunteers without H pylori infection participated in this crossover study comparing lafutidi...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,随机对照试验

    doi:10.3748/wjg.14.2406

    authors: Yamagishi H,Koike T,Ohara S,Horii T,Kikuchi R,Kobayashi S,Abe Y,Iijima K,Imatani A,Suzuki K,Hishinuma T,Goto J,Shimosegawa T

    更新日期:2008-04-21 00:00:00

  • Bile acid formation in primary human hepatocytes.

    abstract::AIM:To evaluate a culture system for bile acid formation in primary human hepatocytes in comparison with HepG2 cells.METHODS:Hepatocytes were isolated from normal human liver tissue and we re cultured in serum-free William's E medium.The medium was collected and re newed every 24 h. Bile acids and their precursors in ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v6.i4.522

    authors: Einarsson C,Ellis E,Abrahamsson A,Ericzon BG,Bjorkhem I,Axelson M

    更新日期:2000-08-01 00:00:00