Abstract:
:AIM:To evaluate a culture system for bile acid formation in primary human hepatocytes in comparison with HepG2 cells.METHODS:Hepatocytes were isolated from normal human liver tissue and we re cultured in serum-free William's E medium.The medium was collected and re newed every 24 h. Bile acids and their precursors in media were finally analysed by gas chromatography mass spectrometry.RESULTS:Cholic acid (CA) and chenodeoxycholic acid (CDCA) conjugated with glycine or taurine accounted for 70% and 25% of total steroids. A third of CDC A was also conjugated with sulphuric acid. Dexamethasone and thyroid hormone alone or in combination did not significantly effect bile acid formation. The addition of cyclosporin A (10&mgr;mol/L) inhibited the synthesis of CA and CDCA by about 13% and 30%, respectively.CONCLUSION:Isolated human hepatocytes in primary culture behave as in the intact liver by converting cholesterol to conjugated CA and CDCA.This is in contrast to cultured HepG2 cells, which release large amounts of bile acid precursors and unconjugated bile acids into the medium.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Einarsson C,Ellis E,Abrahamsson A,Ericzon BG,Bjorkhem I,Axelson Mdoi
10.3748/wjg.v6.i4.522keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
522-525issue
4eissn
1007-9327issn
2219-2840journal_volume
6pub_type
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doi:10.3748/wjg.v25.i43.6440
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doi:10.3748/wjg.14.2794
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.3748/wjg.v20.i10.2641
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pub_type: 杂志文章
doi:10.3748/wjg.v11.i3.426
更新日期:2005-01-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.960
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,随机对照试验
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更新日期:2011-01-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2016-12-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2007-10-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章
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更新日期:2004-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-08-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2016-01-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i31.10658
更新日期:2014-08-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v23.i40.7332
更新日期:2017-10-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i39.6134
更新日期:2005-10-21 00:00:00
abstract:AIM:Trace elements (TE) metabolism is altered in inflammatory bowel diseases. TE (zinc and copper) are constituents of antioxidant enzymes. Iron is involved in the pathogenesis of chronic inflammation. The aim was to evaluate zinc and copper status and the effects of iron manipulation in experimental colitis. METHODS:...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i28.4396
更新日期:2005-07-28 00:00:00