Dual specimens increase the diagnostic accuracy and reduce the reaction duration of rapid urease test.

Abstract:

AIM:To evaluate the influence of multiple samplings during esophagogastroduodenoscopy (EGD) on the accuracy of the rapid urease test, and the validity of newly developed rapid urease tests, HelicotecUT plus test and HelicotecUT test, CLO test and ProntoDry test. METHODS:A total of 355 patients undergoing EGD for dyspepsia were included. Their Helicobacter pylori (H. pylori) treatment status was either naïve or eradicated. Six biopsy specimens from antrum and gastric body, respectively, were obtained during EGD. Single antral specimens and dual (antrum + body) specimens were compared. Infection status of H. pylori was evaluated by three different tests: culture, histology, and four different commercially available rapid urease tests (RUTs)-including the newly developed HelicotecUT plus test and HelicotecUT test, and established CLO test and ProntoDry test. H. pylori status was defined as positive when the culture was positive or if there were concordant positive results among histology, CLO test and ProntoDry test. RESULTS:When dual specimens were applied, sensitivity was enhanced and RUT reaction time was significantly reduced, regardless of their treatment status. Thirty minutes were enough to achieve an agreeable positive rate in all the RUTs. Both newly developed RUTs showed comparable sensitivity, specificity and accuracy to the established RUTs, regardless of patient treatment status, RUT reaction duration, and EGD biopsy sites. CONCLUSION:Combination of antrum and body biopsy specimens greatly enhances the sensitivity of rapid urease test and reduces the reaction duration to 30 min.

journal_name

World J Gastroenterol

authors

Hsu WH,Wang SS,Kuo CH,Chen CY,Chang CW,Hu HM,Wang JY,Yang YC,Lin YC,Wang WM,Wu DC,Wu MT,Kuo FC

doi

10.3748/wjg.v16.i23.2926

subject

Has Abstract

pub_date

2010-06-21 00:00:00

pages

2926-30

issue

23

eissn

1007-9327

issn

2219-2840

journal_volume

16

pub_type

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