Abstract：
AIM:To investigate the mechanisms of aspirin increasing the susceptibility of Helicobacter pylori (H pylori) to metronidazole. METHODS:H pylori reference strain 26695 and two metronidazole-resistant isolates of H pylori were included in this study. Strains were incubated in Brucella broth with or without aspirin (1 mmol/L). The rdxA gene of H pylori was amplified by PCR and sequenced. The permeability of H pylori to antimicrobials was determined by analyzing the endocellular radioactivity of the cells after incubated with [7-(3)H]-tetracycline. The outer membrane proteins (OMPs) of H pylori 26695 were depurated and analyzed by SDS-PAGE. The expression of 5 porins (hopA, hopB, hopC, hopD and hopE) and the putative RND efflux system (hefABC) of H pylori were analyzed using real-time quantitative PCR. RESULTS:The mutations in rdxA gene did not change in metronidazole resistant isolates treated with aspirin. The radioactivity of H pylori increased when treated with aspirin, indicating that aspirin improved the permeability of the outer membrane of H pylori. However, the expression of two OMP bands between 55 kDa and 72 kDa altered in the presence of aspirin. The expression of the mRNA of hopA, hopB, hopC, hopD, hopE and hefA, hefB, hefC of H pylori did not change when treated with aspirin. CONCLUSION:Although aspirin increases the susceptibility of H pylori to metronidazole, it has no effect on the mutations of rdxA gene of H pylori. Aspirin increases endocellular concentrations of antimicrobials probably by altering the OMP expression.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Zhang XP,Wang WH,Tian Y,Gao W,Li Jdoi
10.3748/wjg.15.919subject
Has Abstractpub_date
2009-02-28 00:00:00pages
919-26issue
8eissn
1007-9327issn
2219-2840journal_volume
15pub_type
杂志文章abstract：AIM:To study the expression of ether à go-go (Eag1) potassium channel in colorectal cancer and the relation-ship between their expression and clinico-pathological features. METHODS:The expression levels of Eag1 protein were determined in 76 cancer tissues with paired non-cancerous matched tissues as well as 9 colorect...
journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v13.i8.1257
更新日期：2007-02-28 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,随机对照试验
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更新日期：2019-12-14 00:00:00
abstract：:[This corrects the article on p. 1107 in vol. 21, PMID: 26640344.]. ...
journal_title：World journal of gastroenterology
pub_type： 杂志文章,已发布勘误
doi：10.3748/wjg.v24.i39.4517
更新日期：2018-10-21 00:00:00
abstract：:Unidimensional transient elastography (TE) is a noninvasive technique, which has been increasingly used in the assessment of diffuse liver diseases. This paper focuses on reviewing the existing data on the use of TE in the diagnosis of fibrosis and in monitoring disease progression in alcoholic liver disease, on the f...
journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
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更新日期：2015-11-14 00:00:00
abstract：:A cerebral lipiodol embolism is an extremely rare complication of transcatheter arterial chemoembolization for hepatocellular carcinoma. We present a case of cerebral lipiodol embolism that occurred after the third arterial chemoembolization, report the clinical and radiological findings, and review the medical litera...
journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
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更新日期：2019-05-21 00:00:00
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pub_type： 临床试验,杂志文章
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更新日期：2004-12-15 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v10.i19.2831
更新日期：2004-10-01 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
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更新日期：2004-06-15 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 临床试验,杂志文章
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更新日期：2004-08-01 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v17.i5.677
更新日期：2011-02-07 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v9.i10.2211
更新日期：2003-10-01 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,meta分析,评审
doi：10.3748/wjg.v20.i35.12649
更新日期：2014-09-21 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v19.i21.3354
更新日期：2013-06-07 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v21.i12.3554
更新日期：2015-03-28 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
doi：10.3748/wjg.v16.i30.3780
更新日期：2010-08-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v18.i10.1130
更新日期：2012-03-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,随机对照试验
doi：10.3748/wjg.15.3783
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.15.5409
更新日期：2009-11-21 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v21.i13.4020
更新日期：2015-04-07 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.14.3729
更新日期：2008-06-21 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v11.i6.854
更新日期：2005-02-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v9.i2.288
更新日期：2003-02-01 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v20.i30.10591
更新日期：2014-08-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v9.i3.427
更新日期：2003-03-01 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v19.i38.6416
更新日期：2013-10-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v13.i34.4586
更新日期：2007-09-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v20.i33.11762
更新日期：2014-09-07 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
doi：10.3748/wjg.v13.i22.3056
更新日期：2007-06-14 00:00:00
abstract：AIM:To investigate the association of tumor necrosis factor alpha (TNFα) -G308A polymorphism with different liver pathological changes in treatment-naïve Egyptian patients infected with hepatitis C virus (HCV) genotype 4. METHODS:This study included 180 subjects, composed of 120 treatment-naïve chronic HCV patients wi...
journal_title：World journal of gastroenterology
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更新日期：2016-09-14 00:00:00
