Abstract：
OBJECTIVE:To determine the effects of menopause (aging and E) on vaginal-cervical epithelial paracellular permeability. DESIGN:Experimental, basic clinical research. SETTING:Academic research environment. PATIENT(S):Premenopausal, perimenopausal, and postmenopausal women, aged 35-65 years. INTERVENTION(S):Primary to tertiary cultures of normal human ectocervical epithelial cells on filters. Cells were outgrown from surgically discarded ectocervical minces. MAIN OUTCOME MEASURE(S):Changes in paracellular permeability were determined as changes in transepithelial electrical conductance and pyranine permeability. RESULT(S):[1] Levels of transepithelial electrical conductance and pyranine permeability decreased as women's age advanced. [2] Removal of E from the culture medium decreased paracellular permeability. Treatment of cells in vitro with 10 nmol/L 17beta-E2 increased transepithelial electrical conductance and pyranine permeability, but the effects were additive to the age-related decrease in permeability. [3] Coadministration of 100 nmol/L tamoxifen blocked the E increase in paracellular permeability in cells of both premenopausal and postmenopausal women. CONCLUSION(S):[1] Aging and E deficiency decrease independently vaginal-cervical epithelial paracellular permeability. [2] The E increase in vaginal-cervical epithelial paracellular permeability in cells of postmenopausal women is mediated by the E receptor. [3] The E increase in vaginal-cervical epithelial paracellular permeability in cells of postmenopausal women is masked by age-related increase in the tight junctional resistance, leading to overall decrease in paracellular permeability.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Gorodeski GIdoi
10.1016/s0015-0282(01)02377-9keywords:
subject
Has Abstractpub_date
2001-10-01 00:00:00pages
753-61issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(01)02377-9journal_volume
76pub_type
杂志文章abstract：OBJECTIVE:To determine the prevalence and type of Y chromosome microdeletions in 136 consecutively seen intracytoplasmic sperm injection (ICSI) candidates and in 50 consecutively seen azoospermic men attending an infertility clinic. DESIGN:Controlled clinical study. SETTING:Genetics laboratory and infertility clinic ...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(98)00195-2
更新日期：1998-09-01 00:00:00
abstract：:The role of maternal diabetes as a cause of repetitive early pregnancy loss was evaluated by examining the reproductive performance of 154 diabetic patients in 489 pregnancies. The overall incidence of spontaneous abortion among gestational diabetics (12.7%) and frank diabetics (12.2%) was not significantly different ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)45797-3
更新日期：1981-10-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development. DESIGN:Animal study. SETTING:University laboratory. ANIMAL(S):CD-1 mice. INTERVENTION(S):Immature oocytes were arrested meiotically in medium containing a specific phosphodiester...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.03.041
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:To report preferences of recipient couples for genetic testing of their oocyte donors. DESIGN:Observational report of results from a genetic testing-options form implemented as part of routine care. SETTING:Private practice. PATIENT(S):Recipients and oocyte donors. INTERVENTION(S):Recipient couples comple...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.10.069
更新日期：2008-12-01 00:00:00
abstract：OBJECTIVE:To measure free T, free E2, and sex hormone-binding globulin (SHBG) levels in oligozoospermic men with varicoceles. DESIGN:Retrospective study. PATIENTS:Sixty-four infertile patients with varicoceles. SETTING:A university-based tertiary care referral center. INTERVENTIONS:Assessment of seminal cellular ch...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：OBJECTIVE:To test a sucrose-glycerol cryoprotectant for IUI-ready sperm preparation. DESIGN:Semen aliquots from normozoospermic donors either were subjected to conventional semen freezing (TES and Tris yolk buffer in 7.4% final glycerol) with post-thaw processing or were preprocessed and frozen in HEPES-buffered human...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)81491-4
更新日期：1997-07-01 00:00:00
abstract：OBJECTIVE:To compare oocyte maturity, fertilization rate and cleavage rate after a short and long GnRH agonist (GnRH-a) stimulation protocol and intracytoplasmic sperm injection (ICSI). DESIGN:Retrospective study of 34 sequential ICSI cycles stimulated with a short or long GnRH-a protocol. SETTING:A university-based ...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)57792-9
更新日期：1995-09-01 00:00:00
abstract：OBJECTIVE:To investigate whether components of the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway are present and active in human sperm. DESIGN:Comparative study. SETTING:Reproductive biology department. PATIENT(S):Nine sperm donors. INTERVENTION(S):Sperm were exposed to interfer...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)01896-9
更新日期：2001-08-01 00:00:00
abstract：OBJECTIVE:To determine whether nerve fibers can be detected in the endometrium and myometrium in women with painful uterine fibroids and adenomyosis. DESIGN:A retrospective immunohistochemical study. SETTING:An academic training hospital. PATIENT(S):Thirty-seven women with uterine fibroids and 29 women with adenomyo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.03.026
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVE:To evaluate the results of IVF in women > or = 40 years of age using their own oocytes. DESIGN:Retrospective study. SETTING:Wolfson and Royal Masonic in vitro fertilization units, London, United Kingdom. PATIENT(S):A total of 1,087 IVF cycles were started in women > or = 40 years of age. INTERVENTION(S):M...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00353-7
更新日期：1998-12-01 00:00:00
abstract：OBJECTIVE:To determine the incidence of ovarian hyperstimulation syndrome (OHSS) in a large series of GnRH antagonist-stimulated cycles and to assess the predictive value of E2 and the number of follicles on the day of hCG administration. DESIGN:Prospective cohort study of women undergoing IVF treatment with a GnRH an...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2005.07.1292
更新日期：2006-01-01 00:00:00
abstract：OBJECTIVE:To determine if the solubilized human zona pellucida (ZP)-induced acrosome reaction is mediated by protein kinases. DESIGN:Capacitated spermatozoa were incubated with inhibitors of cyclic adenosine 3':5'-monophosphate (cAMP)-dependent kinase (KT5720), Ca(2+)- and phospholipid-dependent kinase (Calphostin C),...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：OBJECTIVE:To assess whether heart rate variability (HRV) at rest and during sympathetic stimulation is disturbed in patients with different polycystic ovary syndrome (PCOS) phenotypes in comparison to healthy controls. DESIGN:Cross-sectional study. SETTING:University hospital. PATIENT(S):Thirty women with classic, a...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.08.049
更新日期：2013-01-01 00:00:00
abstract：:The effects of intravaginal application of nonoxynol-9 (N-9) on hematological parameters, routine liver function biochemistry (bilirubin, serum bile acid, SGOT, SGPT, GGT, alkaline phosphatase), and serum lipids (cholesterol and triglyceride) were evaluated in 10 normal women who received 150 mg N-9 daily for 14 conse...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/s0015-0282(16)46058-9
更新日期：1982-02-01 00:00:00
abstract：:Expanded carrier screening refers to identification of carriers of single-gene disorders outside of traditional screening guidelines. New genetic testing technologies allow for such screening at costs that are comparable to single-gene testing. There is a high degree of variability among genetic testing laboratories a...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2017.11.030
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVE:To evaluate the effects of laparoscopic bipolar coagulation of uterine vessels in treating symptomatic fibroids. DESIGN:Prospective clinical study. SETTING:University-affiliated tertiary referral center. PATIENT(S):Eighty-seven women with symptomatic fibroids warranting surgical treatment and wanting to re...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)01724-6
更新日期：2001-02-01 00:00:00
abstract：OBJECTIVE:To determine the role of parental and biological factors on the U.S. male birth fraction from 1964 through 1988. DESIGN:Logistic regression on annual U.S. male births by race group. SETTING:Population-based data. PATIENT(S):Live births in the United States 1964 through 1988. INTERVENTION(S):None. MAIN OU...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00576-2
更新日期：2000-03-01 00:00:00
abstract：OBJECTIVE:To determine the prevalence of anticardiolipin and antiphophatidylserine antibodies in an IVF population and to correlate their presence and specific isotype with IVF cycle outcome. DESIGN:Retrospective clinical study using stored midfollicular sera for determination of antibody status. SETTING:University h...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)81519-1
更新日期：1997-08-01 00:00:00
abstract：OBJECTIVE:To assess the utility of endometrial thickness, echogenic endometrial pattern, and uterine artery impedance measured as pulsativity index in predicting implantation. DESIGN:Prospective case-controlled study of infertile patients undergoing assisted reproductive technologies (ARTs). PATIENTS:Four hundred fiv...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)57065-4
更新日期：1994-11-01 00:00:00
abstract：STUDY OBJECTIVES:To assess a single (donor) sample by the use of five computer-assisted semen analysis (CASA) systems. SETTING:British Andrology Society advanced course on computer-assisted semen analysis techniques, September 1992. PARTICIPANTS:Clinical, technical and scientific personnel with mixed experience of CA...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：OBJECTIVE:To compare the efficiency of hysterosalpingo-contrast-sonography performed with air and saline (Hydro-HyCoSy) with HyCoSy performed with contrast media (SonoVue) and contrast-tuned imaging technology (CnTI-SonoVue-HyCoSy) considering hysterosalpingography (HSG) and laparoscopy (LPS) as reference tests and to ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.07.1746
更新日期：2009-09-01 00:00:00
abstract：:Human GCs obtained from patients undergoing an IVF procedure and treated with CC, hMG, and hCG, lack cAMP responsiveness to hCG stimulation in vitro. After 48 hours in culture, recovery of cAMP production was significantly higher in hCG-stimulated cells grown on dishes coated with ECM than in cells grown on uncoated d...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49596-8
更新日期：1986-09-01 00:00:00
abstract：:In this observational, case-control study in infertile women, we observed elevated serum oxidative stress that was significantly correlated with an increase in serum paraoxonase-1 activity and without any evidence of a proinflammatory reaction. These results suggest a protective role of paraoxonase-1 against inflammat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.11.043
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVE:To investigate semen quality in infertile men from an urban population over an 18-year period. DESIGN:Retrospective study. SETTING:Andrology clinic at a city university hospital. PATIENTS:A total of 9,327 men were referred to the clinic for infertility investigation. After excluding those with azoospermia,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.05.049
更新日期：2005-12-01 00:00:00
abstract：OBJECTIVE:To assess the molecular signatures underlying endometrial disorder using cDNA microarray. DESIGN:Gene expression-based oligonucleotide array of the normal endometrium. SETTING:University hospital. PATIENT(S):Humans. INTERVENTION(S):Endometrial tissues were obtained from 28 normal cycling women undergoing ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.01.130
更新日期：2010-06-01 00:00:00
abstract：OBJECTIVE:To study the safety and efficacy of ovarian stimulation and oocyte cryopreservation as a method of fertility preservation in women with Turner syndrome (TS). DESIGN:Retrospective cohort study. SETTING:Reproductive medicine clinic. PATIENT(S):Seven women with TS who attended the clinic between 2011 and 2017...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2018.11.010
更新日期：2019-03-01 00:00:00
abstract：:Defects of the DNA mismatch repair gene hMLH3 were screened by denaturing high-performance liquid chromatography and sequencing in germinal tissue DNA from patients with spermatogenic arrest, with sequence variations being confirmed in genomic DNA by polymerase chain reaction (PCR) direct sequencing analysis. Four mis...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.01.063
更新日期：2007-12-01 00:00:00
abstract：:To show that in the dysgenetic gonads of 104 Turner syndrome patients no significant difference was found regarding the expression of the genes DAX1, FOG2, GATA4, OCT4, SF1, SRY, TSPY, WT1, and STRA8 compared with controls, except for genes OCT4, SRY, and TSPY in both gonads of a patient whose chromosomal constitution...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.02.036
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:Racial disparity in assisted reproductive technology (ART) outcomes has been reported but remains controversial. Reasons for the disparity are unclear, and access to care has been suggested as a causative factor. In this study, we sought to examine minority utilization of ART in the Department of Defense (DoD...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.10.028
更新日期：2006-04-01 00:00:00
abstract：:Preimplantation genetic diagnosis has been proposed as a method to improve assisted reproduction technology outcomes, but different techniques have produced conflicting results. The use of appropriate techniques may provide positive outcomes. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2009.02.091
更新日期：2010-07-01 00:00:00
