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Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development.

Abstract：

OBJECTIVE:To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development. DESIGN:Animal study. SETTING:University laboratory. ANIMAL(S):CD-1 mice. INTERVENTION(S):Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 micromol/L, 5 micromol/L, and 10 micromol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium. MAIN OUTCOME MEASURE(S):Oocyte maturation, fertilization, and early embryonic development in vitro. RESULT(S):After 6 and 24 hours of prematuration culture with 1 micromol/L, 5 micromol/L, and 10 micromol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls. CONCLUSION(S):Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Jee BC,Chen HY,Chian RC

doi

10.1016/j.fertnstert.2008.03.041

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

2037-42

issue

5 Suppl

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(08)00708-5

journal_volume

91

pub_type

杂志文章

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