Abstract：
:We evaluated cell survival, DNA double strand breaks (dsbs), and DNA synthesis following camptothecin (CPT) alone or concurrent CPT and X-radiation treatments in exponential-phase cultures of a radioresistant human melanoma cell line. Cell survival was measured by a clonogenic assay. DNA dsbs were measured by pulsed-field gel electrophoresis. DNA synthesis was measured by incorporation of (3)H-thymidine. We found that (i) concurrent CPT and X-radiation interacted additively, unlike previous results with plateau-phase cultures of these cells, which showed synergistic interaction; (ii) there were strong negative correlations (correlation coefficients of at least 0.82) between clonogenic surviving fractions and DNA dsbs following CPT alone or concurrent CPT and radiation treatments; and (iii) concurrent CPT and radiation (10 Gy) treatment did not completely inhibit DNA synthesis, even though addition of radiation to CPT did further decrease DNA synthesis (relative to CPT alone) at CPT concentrations below 20 microM. Our results suggest that during concurrent CPT and radiation treatment residual DNA dsb levels were good indicators of cell killing and that the absence of complete inhibition of DNA synthesis could at least in part explain the additive interaction between CPT and radiation.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Ng CE,Mazaheri K,Payant C,Raaphorst GPdoi
10.1002/ijc.1031keywords:
subject
Has Abstractpub_date
2001-10-20 00:00:00pages
277-85issue
5eissn
0020-7136issn
1097-0215pii
10.1002/ijc.1031journal_volume
96pub_type
杂志文章abstract：:Meningioma, the most frequent tumor in the central nervous system, has few recognized risk factors. We explored the role of allergies in a population-based case-control consortium study of meningioma in five geographic areas. We also studied serum levels of a marker of atopic allergy (IgE) in a subset of study partici...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.25858
更新日期：2011-10-15 00:00:00
abstract：:Socioeconomic status (SES) is a determinant of outcome in various types of cancer. The aim of this study is to analyze the impact of the SES in Hodgkin's lymphoma (HL). From 2001 to 2005, 194 consecutive patients were prospectively followed in 5 institutions. Patients answered a questionnaire with a set of items used ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22417
更新日期：2007-02-15 00:00:00
abstract：:Leukocyte migration inhibition (LMI) assays were performed using 3 M KCI extracts to detect cell-mediated immune reactions against tumor-associated antigens (TAA) of swine melanoma. Positive LMI reactivity was 70% or greater in melanoma-bearing swine compared to 10% reactivity or less in normal swine tested with extra...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910310516
更新日期：1983-05-15 00:00:00
abstract：:Our previous study has suggested that the cell cycle-related kinase (CCRK) is a putative candidate oncogene in glioblastoma tumorigenesis. The potential oncogenic role of CCRK and its clinical/prognostic significance, however, in ovarian carcinoma are unclear. In this study, CCRK expression was examined by immunohisto...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.24630
更新日期：2009-12-01 00:00:00
abstract：:In colorectal cancer (CRC), hepatic arterial infusion (HAI) chemotherapy may convert primarily unresectable CRC liver metastases (CLM) into resectability, although the risk of metastatic recurrence remains high after CLM ablation. We investigated the role of antitumour immunity invoked by first-line oxaliplatin-HAI fo...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32847
更新日期：2020-04-01 00:00:00
abstract：:Previous studies indicate that the triterpene glycoside actein from the herb black cohosh inhibits growth of human breast cancer cells. This study seeks to identify genes altered in human breast cancer cells by treatment with actein, using gene expression analysis. We treated MDA-MB-453 human breast cancer cells with ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22897
更新日期：2007-11-01 00:00:00
abstract：:Lethal phenotypes of human prostate cancer are characterized by progression to androgen-independence and metastasis. For want of a clinically relevant animal model, mechanisms behind this progression remain unclear. Our study used an in vivo model of androgen-sensitive LNCaP human prostate cancer cell xenografts in ma...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20206
更新日期：2004-07-20 00:00:00
abstract：:The effect of serum from mice bearing methylcholanthrene-induced fibrosarcomas on the lympho-proliferative responses of their spleen and lymph-node cells to 3 M KCl-solubilized tumor antigens has been investigated. Low concentrations of serum (less than or equal to 2.5%) collected throughout the course of tumor growth...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910200312
更新日期：1977-09-15 00:00:00
abstract：:Dendritic cells (DC) have gained much interest in the field of anticancer vaccine development because of their central function in immune regulation. However, the clinical application of ex vivo cultured DC has significant disadvantages. A vaccine that targets dendritic cells in vivo and enhances antigen presentation ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23101
更新日期：2008-02-15 00:00:00
abstract：:Professional antigen-presenting cells (APC), e.g. dendritic cells, express immuno-proteasome components and process proteins for MHC presentation differently from non-immune cells. Thus, they induce reactivities against sets of peptides that do not overlap with those generated by non-professional APC, i.e., tumor cell...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.10616
更新日期：2002-10-01 00:00:00
abstract：:Pre-malignant and malignant human colorectal tumour epithelial cell lines both secreted precursor forms of the 2 cysteine proteinases, cathepsins B and L. The amount of proteinases secreted by these cell lines varied according to the cell density. Comparison at similar cell densities showed that the pre-malignant, ade...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910430323
更新日期：1989-03-15 00:00:00
abstract：:Hepatocellular carcinoma (HCC) development entails changes in liver metabolism. Current knowledge on metabolic perturbations in HCC is derived mostly from case-control designs, with sparse information from prospective cohorts. Our objective was to apply comprehensive metabolite profiling to detect metabolites whose se...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.33236
更新日期：2021-02-01 00:00:00
abstract：:A panel of 7 mouse monoclonal antibodies and the lectin from Ulex europeus, detecting blood-group-related antigens of the ABH and Lewis systems, have been used to define the distribution of these antigenic structures within the human gastrointestinal tract, and to characterize their expression and modulation in colore...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910370505
更新日期：1986-05-15 00:00:00
abstract：:We treated 72 patients with metastatic renal-cell cancer according to 2 protocols consisting of two 5-week induction cycles of continuous i.v. high-dose interleukin-2 (IL-2), i.m. interferon-alpha (IFN alpha) and ex vivo IL-2-activated lymphocytes, followed for patients with stable disease (SD), partial response (PR) ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(SICI)1097-0215(19960117)65:2<152::AID-IJC
更新日期：1996-01-17 00:00:00
abstract：:Elevated platelet counts in patients diagnosed with malignant tumors were first described more than 100 years ago. Today it is well known that in many types of solid tumors, thrombocytosis at the time of diagnosis is associated with shorter survival. From this well-documented clinical correlation between platelet coun...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.27441
更新日期：2012-06-15 00:00:00
abstract：:It has been suggested that smoking is associated with estrogen-negative breast cancer but not with estrogen-positive breast cancer. A population-based case-control study was conducted in Geneva, Switzerland, to determine the relation of passive and active smoking to breast cancer when the referent unexposed category c...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19980130)75:3<339::aid-ijc
更新日期：1998-01-30 00:00:00
abstract：:Quality of life outcomes provide essential information for patients and physicians in oncology care. However, the validity of progression-free survival (PFS) as a surrogate for quality of life, and the inclusion and reporting of quality of life endpoints in clinical trials, is unclear. We performed a retrospective stu...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31957
更新日期：2019-04-01 00:00:00
abstract：:Tumor-induced immunosuppression remains a major challenge for immunotherapy of cancer patients. To further elucidate why an allogeneic gene-modified [interleukin-7 (IL-7)/CD80-cotransfected] renal cell cancer (RCC) vaccine failed to induce clinically relevant TH-1-polarized immune responses, peripheral blood mononucle...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.29230
更新日期：2015-04-15 00:00:00
abstract：:Hypoxia is known to play important roles in the development and progression of tumors. We previously demonstrated that S100A4, a critical molecule for metastasis, was upregulated in ovarian cancer cells. Therefore, we examined the mechanisms of the upregulation of S100A4 expression in ovarian carcinoma cells, with par...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.27448
更新日期：2012-10-15 00:00:00
abstract：:Improved biomarkers for prostate cancer (PC) risk stratification are urgently needed. Here, we aimed to develop a novel multimarker model for prediction of biochemical recurrence (BCR) after curatively intended radical prostatectomy (RP), based on minimally invasive sampling of blood and urine. We initially measured t...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32296
更新日期：2019-11-01 00:00:00
abstract：:Immunochromatographical fecal occult blood tests were shown to have higher sensitivity for detecting colorectal neoplasms than the commonly used guaiac-based test. However, positivity rates, sensitivity and specificity vary widely. We aimed to assess the reasons for this heterogeneity. Six dichotomous (qualitative) im...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.25154
更新日期：2010-10-01 00:00:00
abstract：:The enzyme nitroreductase from E. coli can reduce the weak, monofunctional alkylating agent 5-(aziridin-1-yl)-2, 4-dinitrobenzamide (CB1954) to a potent cytotoxic species that generates interstrand crosslinks in DNA. Nitroreductase therefore has potential as a "suicide enzyme" for cancer gene therapy, as cells that ex...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(20000615)86:6<848::aid-ijc
更新日期：2000-06-15 00:00:00
abstract：:Human epidermoid carcinoma A431 cells attach more rapidly to collagen type-I and -IV substrates than to surfaces coated with laminin or fibronectin. The diminished intercellular interaction and rounding up manifested when these cells are exposed to epidermal growth factor (EGF) in tissue culture plastic or collagen fi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910370313
更新日期：1986-03-15 00:00:00
abstract：:Mouse-transformed keratinocytes cultured in the presence of transforming growth factor beta1 (TGF-beta1) acquire an array of morphologic and functional properties that give rise to a migratory phenotype that expresses mesenchymal molecular markers. This cellular conversion involves activation of the Ras-ERK pathway, e...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.11457
更新日期：2003-12-10 00:00:00
abstract：:High dietary intakes of calcium and dairy products have been hypothesized to enhance prostate cancer risk, but available prospective data regarding these associations are inconsistent. We examined dietary intakes of calcium and dairy products in relation to risk of prostate cancer in the Alpha-Tocopherol, Beta-Caroten...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22553
更新日期：2007-06-01 00:00:00
abstract：:The aim of this study was to determine whether the skin-sensitizing agent 2,4-dinitrofluorobenzene (DNFB) would elicit the same morphological and biochemical events that are characteristic of 12-O-tetradecanoylphorbol-13-acetate (TPA). While single applications of 0.1% or 0.2% DNFB produced only mild epidermal hyperpl...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910600420
更新日期：1995-02-08 00:00:00
abstract：:The treatment of human peripheral blood mononuclear cells (PBMC) with micromolar concentrations of SV-IV, a major protein secreted from the rat seminal vesicle epithelium, promotes in this cell population a marked cytotoxic activity against the Raji lymphoblastoid cell line. This activity is apparently due to cell-to-...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19970717)72:2<321::aid-ijc
更新日期：1997-07-17 00:00:00
abstract：:Exercise has been shown to reduce risk and improve prognosis of several types of cancers. Irisin is a myokine linked to exercise and lean body mass, which is thought to favorably alter metabolism systemically, potentially providing benefit for metabolic disease (including cancer). We evaluated the effects of various c...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.29142
更新日期：2015-02-15 00:00:00
abstract：:Cytotoxic antibodies against glioblastoma-associated antigen(s) have been sought for in glioma patient sera. Complement-dependent cytotoxicity (CDC) I and antibody-dependent cell-mediated cytotoxicity (ADCC) assays were used to test sera from 80 patients using 51 Cr labelled target cells derived from eight different g...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910250209
更新日期：1980-02-15 00:00:00
abstract：:Nude athymic rats of Rowett genetic background were injected subcutaneously with cells from a poorly differentiated human pulmonary adenocarcinoma, following propagation in nude mice. The tumour cells were obtained by thoracentesis and by pleural biopsy during thoracoscopy. So far, we have heterotransplanted 13 differ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910320621
更新日期：1983-12-15 00:00:00
