Abstract:
:Nude athymic rats of Rowett genetic background were injected subcutaneously with cells from a poorly differentiated human pulmonary adenocarcinoma, following propagation in nude mice. The tumour cells were obtained by thoracentesis and by pleural biopsy during thoracoscopy. So far, we have heterotransplanted 13 different malignant human tumours including malignant mesothelioma, pulmonary adenocarcinoma, malignant melanoma and malignant lymphoma. Subcutaneous tumour growth was seen in all inoculated animals, with an exponential tumour growth pattern and with tumour volume doubling times of approximately 8 days. After two initial passages in nude athymic mice of BALB/c genetic background, the human tumour so far has undergone 9 passages in athymic rats, i.e. collection of tumour material by biopsy from the preceding tumour-bearing rat generation, processing and inoculation of tumour brei with subsequent tumour growth have been repeated 8 times. Four-to-8-week-old rats of both sexes were used throughout and tumour growth was controlled by palpation twice a week. Routine histopathological sections were prepared from the tumour at various passages to assess similarity to the original tumour in the patient. The growth pattern of the xenografts remained similar at all passages just as the remarkable similarity of the heterotransplanted tumours to the original adenocarcinoma was retained at all passages. No spontaneous regressions of heterotransplanted tumours could be demonstrated. Electron microscopical analysis revealed numerous blunt microvilli and lumina partly filled with conglomerates of mucigen granules and small glycocalyceal bodies associated with external superficial microvilli. Scantiness of dark secretory granules together with free and membrane-bound polyribosomes were seen in the cytoplasm. We believe that the nude athymic rat is a valuable research tool and that the permanently transplantable human tumour reported here could be of value in delineating further the mechanisms for tumour take, growth and control.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Korsgaard R,Lindén CJ,Willén R,Willén H,Svensson G,Simonsson BGdoi
10.1002/ijc.2910320621subject
Has Abstractpub_date
1983-12-15 00:00:00pages
793-9issue
6eissn
0020-7136issn
1097-0215journal_volume
32pub_type
杂志文章abstract::Cultured tumor cell lines, tumor xenografts grown in athymic nude mice, and a murine experimental metastasis model were used to assess the in vitro and in vivo anti-tumor activity of the potent IMP dehydrogenase (IMPDH) inhibitor, mycophenolic acid (MPA), and its morpholinoethyl ester pro-drug, mycophenolate mofetil (...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570421
更新日期:1994-05-15 00:00:00
abstract::ARID1A has been identified as a novel tumor suppressor gene in ovarian cancer and subsequently in various other tumor types. ARID1A belongs to the ARID domain containing gene family, which comprises of 15 genes involved, for example, in transcriptional regulation, proliferation and chromatin remodeling. In this study,...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28705
更新日期:2014-08-01 00:00:00
abstract::The ability of verapamil to overcome resistance to adriamycin in a multidrug-resistant derivative of the V79 cell line (LZ), grown as multicellular spheroids or as monolayers, was examined. Verapamil was much less effective in overcoming resistance to adriamycin in spheroids than in monolayers. Verapamil increased the...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910590222
更新日期:1994-10-15 00:00:00
abstract::The aim of this study was to identify pathways that have a significant impact during renal carcinogenesis. Sixty-seven paired samples of both noncancerous renal cortex tissue and cancerous tissue from patients with clear cell renal cell carcinomas (RCCs) were subjected to whole-exome, methylome and transcriptome analy...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28768
更新日期:2014-09-15 00:00:00
abstract::Cervical cancer screening will rely, increasingly, on HPV testing as a primary screen. The requirement for triage tests which can delineate clinically significant infection is thus prescient. In this EUROGIN 2017 roadmap, justification behind the most evidenced triages is outlined, as are challenges for implementation...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
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更新日期:2018-08-15 00:00:00
abstract::An antigen, protein X (Px), was purified from immune complexes isolated from malignant pleural effusions from patients with adenocarcinoma of the lung by EDTA treatment, PEG 8000 precipitation, protein A affinity chromatography, and Sephadex G-200 separation in the presence of 3 M NaCl. The purified antigen had a M(r)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520404
更新日期:1992-10-21 00:00:00
abstract::By centrifuging total cellular DNA derived from human genital warts (condylomata acuminata) in CsCl-ethidium bromide gradients, supercoiled DNA was isolated. The molecular weight of this DNA was determined by agarose gel electrophoresis and amounted to 5.1 X 10(4). This DNA isolated from an individual genital wart was...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910250509
更新日期:1980-05-15 00:00:00
abstract::9-cis-Retinoic acid (9cRA) and 1alpha,25-dihydroxyvitamin D3 (1,25D) show promise as potential chemopreventive agents. We examined 9cRA and 1,25D, alone and in combination, for their potential to inhibit carcinogen (NNK)-induced lung carcinogenesis in A/J mice. A/J mice (n=14/group) were treated with 9cRA (7.5, 15, or...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22462
更新日期:2007-04-01 00:00:00
abstract::Altered microRNA (miRNA) expression has been found to promote carcinogenesis, but little is known about the role of miRNAs in esophageal cancer. In this study, we selected 10 miRNAs and analyzed their expression in 10 esophageal cancer cell lines and 158 tissue specimens using Northern blotting and in situ hybridizati...
journal_title:International journal of cancer
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doi:10.1002/ijc.25330
更新日期:2011-01-01 00:00:00
abstract::BW 5147 lymphoma cells are non-invasive tumor cells which do not generate experimental metastases following i.v. inoculation. In contrast, s.c. and intra-splenic (i.s.) growth of BW cells resulted in widespread colonization of liver and spleen. Cells derived from either s.c. tumors or metastatic lesions did generate m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910410514
更新日期:1988-05-15 00:00:00
abstract::Lymphocyte blastogenesis induced by autologous tumor biopsy cells, by allogeneic lymphocytes and by PHA, was performed in the presence of autologous or allogeneic serum collected from normal blood donors. In 13 of the 15 cases lymphocyte stimulation by autologous tumor cells was inhibited in the presence of autologous...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910160517
更新日期:1975-11-15 00:00:00
abstract::We have analysed expression of the first 7 members of the family of heparin-binding fibroblast growth factor (FGFs) and their 4 high-affinity receptors (FGFRs) in human pancreatic carcinoma cell lines, both at the mRNA and protein levels. In cell lines expressing FGFRs, 2 typical patterns were observed: (i) expression...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910590515
更新日期:1994-12-01 00:00:00
abstract::Squamous cell carcinoma of the conjunctiva (SCCC) has been associated with HIV infection in equatorial Africa, but the evidence for association with HIV in developed countries, where SCCC is rarer, is controversial. We investigated the risk for SCCC and other eye cancers in the updated U.S. HIV/AIDS Cancer Match Regis...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23384
更新日期:2008-06-01 00:00:00
abstract::Lysine-specific demethylase-1 (LSD1) removes the methyl groups from mono- and di-methylated lysine 4 of histone H3. Previous studies have linked LSD1 to malignancy in several human tumors, and LSD1 is considered to epigenetically regulate the energy metabolism genes in adipocytes and hepatocellular carcinoma. This stu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29714
更新日期:2016-01-15 00:00:00
abstract::Serum alkaline DNase activity (SADA) was investigated in rats receiving s.c. transplants of tumor cells sensitive or resistant to chemotherapy. Serum samples from each animal were collected before transplantation, during the development of tumors and after therapy. Within a few days after transplantation of both tumor...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430538
更新日期:1989-05-15 00:00:00
abstract::We used oligonucleotide microarrays to investigate gene expression changes associated with multi-step human papillomavirus type 16 (HPV16)-mediated carcinogenesis in vitro. Gene expression profiles in 4 early passage HPV16-immortalized human keratinocyte (HKc) lines derived from different donors were compared with the...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23463
更新日期:2008-07-01 00:00:00
abstract::Apoptosis protease-activating factor 1 (APAF-1), a transcriptional target of p53, is a cytosolic adaptor protein that links the mitochondrial apoptosis pathway to the caspase cascade. Here, we aimed to study the impact of APAF-1 expression levels on cell death induced by anticancer drugs or ionizing irradiation (IR) a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21535
更新日期:2006-05-01 00:00:00
abstract::The numbers of new cancer cases in 16 common sites occurring in 1980 have been estimated for 24 areas of the world for which the United Nations produces population estimates. For the world as a whole, the total number of new cases was 6.35 million, almost exactly evenly divided between the developed and developing cou...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910410205
更新日期:1988-02-15 00:00:00
abstract::Breast carcinomas were examined by the immunoperoxidase technique using antisera specific for lymphocyte subsets, monocytes, NK cells and major histocompatibility antigens (HLA-A, -B, -C; Ia-like). Sixty-four per cent of the patients had a moderate or strong mononuclear cell infiltration, 77% of the patients without m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910350610
更新日期:1985-06-15 00:00:00
abstract::Clinical case reports have suggested that specific bacterial infections are associated with certain non-Hodgkin lymphoma (NHL) subtypes. Epidemiological case-control studies have been conducted using antibiotics as a proxy for bacterial infections, but with inconclusive results. The aim of this study was, in a cohort ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27626
更新日期:2012-10-01 00:00:00
abstract::Antitumor immunity in chronic lymphocytic leukemia (CLL) is hampered by highly dysfunctional T-cells. Although certain T-cell subsets have been reported to be of prognostic significance in this disease, their interplay is complex and it remains incompletely understood which of these subsets significantly drive CLL pro...
journal_title:International journal of cancer
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doi:10.1002/ijc.28884
更新日期:2014-11-15 00:00:00
abstract::A new human cell line, designated Ty-82, was established from the pleural effusion of a 22-year-old woman with undifferentiated thymic carcinoma. This cell line consisted of primitive cells that were positive for alpha-naphthyl butyrate esterase and acid phosphatase. The cells were shown to express epithelial membrane...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910500216
更新日期:1992-01-21 00:00:00
abstract::We derived from blood lymphocytes of a melanoma patient a large number of cytolytic T-cell clones directed against a cell line of the autologous tumor. Three distinct groups of antigens were recognized by these CTL on the autologous melanoma cells: group A consisted of stable antigens present on all sublines, whereas ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440413
更新日期:1989-10-15 00:00:00
abstract::Rab11-FIP2 can interact with MYO5B and plays an important role in regulating plasma membrane recycling. Our previous study has shown that MYO5B is epigenetically silenced and associated with c-Met signaling in human gastric cancer. However, little is known of the function of Rab11-FIP2 in gastric cancer. In this study...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29899
更新日期:2016-04-01 00:00:00
abstract::Indoor air pollution (IAP) caused by cooking has been associated with lung cancer risk in retrospective case-control studies in developing and rural countries. We report the association of cooking conditions, fuel use, oil use, and risk of lung cancer in a developed urban population in a prospective cohort of women in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29020
更新日期:2015-02-01 00:00:00
abstract::Malignant tumors are generally characterized by extensive local tissue invasion and destruction of ECM which may be due to increased constitutive expression and activity of secreted proteases. Moreover, a large number of diverse protease activities may be constitutively over-expressed in a simultaneous or co-ordinated...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480413
更新日期:1991-06-19 00:00:00
abstract::Overuse of antibiotic drugs alters the composition of gut microbiota and has detrimental effects on the host. In our study, we investigated association of gut flora and antibiotics in the prognosis of patients with liver cancer who have undergone chemotherapy by analyzing two independent clinical studies. We retrospec...
journal_title:International journal of cancer
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doi:10.1002/ijc.32339
更新日期:2019-11-15 00:00:00
abstract::Ocular adnexal MALT lymphoma (OAML) is linked to Chlamydophila psittaci (Cp) infection. Viability and infectivity of Cp, demonstrated by growth in culture, has not been yet investigated in these patients. We conducted a single-center prospective case-control study to assess the prevalence, viability and infectivity of...
journal_title:International journal of cancer
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doi:10.1002/ijc.23596
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abstract::The transcription factor PAX3 is expressed during early embryogenesis and in multiple cancer types, including embryonal rhabdomyosarcoma (ERMS), Ewing sarcoma (ES) and malignant melanoma (MEL), suggesting that it could function as a general tumor associated antigen. Major histocompatibility complex (MHC) peptide bindi...
journal_title:International journal of cancer
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doi:10.1002/ijc.21817
更新日期:2006-07-01 00:00:00
abstract::Histologic characteristics of bladder cancer in Boston, USA, Manchester, UK, and Nagoya, Japan, were evaluated. In each of these areas broadly-based series of cases were assembled during a collaborative case-control study. The present analysis was based on 589 cases in Boston, 484 cases in Manchester, and 241 cases in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910300605
更新日期:1982-12-15 00:00:00