Abstract:
:We used oligonucleotide microarrays to investigate gene expression changes associated with multi-step human papillomavirus type 16 (HPV16)-mediated carcinogenesis in vitro. Gene expression profiles in 4 early passage HPV16-immortalized human keratinocyte (HKc) lines derived from different donors were compared with their corresponding 4 late-passage, differentiation-resistant cell lines, and to 4 pools of normal HKc, each composed of 3 individual HKc strains, on Agilent 22 k human oligonucleotide microarrays. The resulting data were analyzed using a modified T-test coded in R to obtain lists of differentially expressed genes. Gene expression changes identified in this model system were then compared with gene expression changes described in published studies of cervical intraepithelial neoplasia (CIN) and cervical cancer. Common genes in these lists were further studied by cluster analysis. Genes whose expression changed in the same direction as in CIN or cervical cancer (concordant) at late stages of HPV16-mediated transformation in vitro formed one major cluster, while those that changed in the opposite direction (discordant) formed a second major cluster. Further annotation found that many discordant expression changes involved gene products with an extracellular localization. Two novel genes were selected for further study: overexpression of SIX1 and GDF15, observed during in vitro progression in our model system, was confirmed in tissue arrays of cervical cancer. These microarray-based studies show that our in vitro model system reflects many cellular and molecular alterations characteristic of cervical cancer, and identified SIX1 and GDF15 as 2 novel potential biomarkers of cervical cancer progression.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Wan F,Miao X,Quraishi I,Kennedy V,Creek KE,Pirisi Ldoi
10.1002/ijc.23463subject
Has Abstractpub_date
2008-07-01 00:00:00pages
32-40issue
1eissn
0020-7136issn
1097-0215journal_volume
123pub_type
杂志文章abstract::Malignant gliomas recur even after extensive surgery and chemo-radiotherapy. Although a relatively novel chemotherapeutic agent, temozolomide (TMZ), has demonstrated promising activity against gliomas, the effects last only a few months and drug resistance develops thereafter in many cases. It has been acknowledged th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25985
更新日期:2012-01-15 00:00:00
abstract::Increasing evidence indicates that breast cancer pathogenesis is linked with DNA double-strand break (DSB) repair dysfunction. This conclusion is based on advances in the study of functions of breast cancer susceptibility genes such as BRCA1 and BRCA2, on the identification of breast cancer-associated changes regardin...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23551
更新日期:2008-08-01 00:00:00
abstract::Mutant KRAS provides a driving force for enhancement of cancer stem cells (CSCs) characteristics contributing transformation of colorectal cancer (CRC) cells harboring adenomatous polyposis coli (APC) mutations. Here, we identified the factors mediating the promotion of CSCs properties induced by KRAS mutation through...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32728
更新日期:2020-05-15 00:00:00
abstract::A population-based case-control study of risk factors for renal-cell carcinoma was conducted in Denmark from 1989 to 1992. A total of 368 histologically verified cases and 396 controls were included. Information on weight, height, physical activity and reproductive factors were collected in a structured interview, alo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910560113
更新日期:1994-01-02 00:00:00
abstract::We have studied the clonal heterogeneity of 2 spontaneously metastasizing mammary carcinomas which recently arose spontaneously in C3H/He female retired breeders. Cells of early (2nd to 5th) transplant generations of these tumors were cloned by a combination of semi-solid agarose colony formation and limiting dilution...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910350220
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abstract::Immunological studies are presented on a patient with a long clinical history suggesting the existence of a tumor-specific immune response. His tumor, first considered benign, progressed to a highly malignant osteosarcoma. Cell-mediated immune reactivity against biopsy cells and against tumor extract was detected in v...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180304
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abstract::A G to T mutation at nucleotide position 2128 in the human FGFR3b coding region resulting in a Cys for Gly substitution (G697C) in the tyrosine kinase domain was observed in 62% (44/71) of oral squamous cell carcinomas (OSCC) examined. Immunostained FGFR3b was found in the cytoplasm of prickle cells in normal epitheli...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21145
更新日期:2005-10-20 00:00:00
abstract::The stability of a cloned murine tumor for sensitivity to NR was examined following growth in vivo in order to test the hypothesis that tumor progression proceeds through the generation and selection of variants. Clonal sensitivity to the [131I]-dUrd elimination assay of NR was assessed for the L5178Y-F9 tumor grown i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910310116
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abstract::Expression of the candidate metastasis-suppressor gene nm23-H1 has been shown to correlate inversely with metastatic potential in some human tumors, but not in all. Until now, few studies have been carried out on the activity of the homologous nm23-H2 gene in human cancer. No nm23 transcription studies exist for human...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550306
更新日期:1993-09-30 00:00:00
abstract::Residential proximity to electrical power lines of different voltage in relation to childhood leukemia was investigated through a case-control study undertaken in Greece during 1993-1994. The study comprised 117 incident cases of childhood leukemia and 202 age-, gender- and place-of-residence-matched controls. Four me...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19971104)73:3<345::aid-ijc
更新日期:1997-11-04 00:00:00
abstract::Little research has been conducted on the long-term value of human papillomavirus (HPV) testing after conization. We investigated whether cytology adds to the value of a negative HPV test for long-term prediction of cervical intraepithelial neoplasia grade 2 or worse (CIN2+). In addition, we compared risk of CIN2+ fol...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29673
更新日期:2015-12-15 00:00:00
abstract::Isothiocyanates (ITC) in cruciferous vegetables may be chemopreventive against gastric cancer development. Glutathione S-transferases (GSTs) may modify the chemopreventive effect of ITC. The relationship between urinary total ITC and risk of gastric cancer was prospectively examined. Between 1986 and 1989, 18,244 midd...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24583
更新日期:2009-12-01 00:00:00
abstract::In a previous study, human ZNF312b was identified as a cell proliferation-associated oncogene via the K-ras/extracellular signal-regulated kinase cascade in gastric cancer. However, the mechanism concerning its transcriptional activation remains unknown. Here, we show that DNA methylation and histone acetylation of th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25871
更新日期:2011-11-01 00:00:00
abstract::Biopsies obtained from 74 Tunisian women with breast cancer (33 cases), benign breast disease (17 cases), and cervical cancer (24 cases) were assayed for the presence of an antigen cross-reacting with gp52 of the mouse mammary tumor virus (MMTV) in order to determine the frequency and possible prognostic significance ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910330305
更新日期:1984-03-15 00:00:00
abstract::The role of age at menarche and at menopause on epithelial ovarian cancer risk was re-assessed in a combined analysis of 3 hospital-based case-control studies conducted in Italy, the United Kingdom and Greece, which produced a total of 1,140 cases and 2,724 controls. In the overall dataset, there was no evidence of an...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490111
更新日期:1991-08-19 00:00:00
abstract::Telomerase activity is considered to be a diagnostic marker of malignancy since most malignant cells express this activity and most somatic cells do not. However, the detection of telomerase activity is rather complicated and is affected by many factors. Recently, human telomerase components were cloned and found to c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990105)80:1<1::aid-ijc1>
更新日期:1999-01-05 00:00:00
abstract::A diet high in linoleic acid (an ω-6 PUFA) increased the formation of miscoding etheno (ε)--DNA adducts in WBC-DNA of women, but not in men (Nair et al., Cancer Epidemiol Biomark Prev 1997;6:597-601). This gender specificity could result from an interaction between ω-6 PUFA intake and estrogen catabolism, via redox-cy...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27479
更新日期:2012-11-01 00:00:00
abstract::PGP9.5 is a controversial molecule from an oncologic point of view. We recently identified frequent methylation of PGP9.5 gene exclusively in primary head and neck squamous cell carcinoma (HNSCC), suggesting that it could be a tumor suppressor gene. On the other hand, PGP9.5 was reported to be overexpressed in a subse...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23354
更新日期:2008-08-15 00:00:00
abstract::A wide variety of primary and metastatic human neoplasms express somatostatin receptors (SS-Rs). We evaluated the SS-R status of malignant lymphomas that had been surgically removed from 31 patients by use of in vitro SS-R autoradiography with the SS analog 125I-[Tyr3]-octreotide as radioligand. Of 11 low-grade-malign...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910500613
更新日期:1992-04-01 00:00:00
abstract::Chloroethylene oxide and 2-chloroacetaldehyde, two possibly carcinogenic metabolities of vinyl chloride in mammals, caused a dose-dependent induction of 8-azaguanine- and ouabain-resistant mutants in Chinese hamster V79 cells in vitro. Up to one-hundred-fold higher concentrations of 2-chloroethanol or monochloroacetic...
journal_title:International journal of cancer
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doi:10.1002/ijc.2910160414
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abstract::To further examine the association between oral hygiene and esophageal squamous cell carcinoma (ESCC) risk and the effect modification of other exposures, we conducted a population-based case-control study between 2010 and 2012 in Taixing, China, a high-risk area for ESCC. Cases were primarily recruited from endoscopy...
journal_title:International journal of cancer
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更新日期:2017-02-01 00:00:00
abstract::This article describes the characteristics of 3 dominant features of breast cancer epidemiology. These characteristics include the association of disease risk with childbearing, its relationship to ovarian activity and its international variation (particularly as the latter differs in the years before and after menopa...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
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更新日期:2006-05-15 00:00:00
abstract::A panel of human breast cancer specimens was examined for single base change mutations by DNA sequencing and for larger deletions using a PCR-based assay. In the cancer specimens examined, no sequencing variants were detected other than a previously characterized polymorphism. Although most of the specimens contained ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20001015)88:2<209::aid-ijc10>3.0
更新日期:2000-10-15 00:00:00
abstract::We and others have previously shown that in vitro conversion of Epstein-Barr virus (EBV)-negative Burkitt lymphoma (BL) cell lines with the immortalizing B95-8 strain of EBV results in a marked elevation in levels of c-fgr proto-oncogene mRNA. We now show, using a nuclear run-off assay, that this induction results fro...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910450222
更新日期:1990-02-15 00:00:00
abstract::The aim of our study was to develop and validate a machine learning algorithm to predict response of individual HER2-amplified colorectal cancer liver metastases (lmCRC) undergoing dual HER2-targeted therapy. Twenty-four radiomics features were extracted after 3D manual segmentation of 141 lmCRC on pretreatment portal...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33271
更新日期:2020-12-01 00:00:00
abstract::Breast cancer is the leading cancer diagnosis and second most common cause of cancer deaths in sub-Saharan Africa (SSA). Yet, there are few population-level survival data from Africa and none on the survival differences by stage at diagnosis. Here, we estimate breast cancer survival within SSA by area, stage and count...
journal_title:International journal of cancer
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更新日期:2020-03-01 00:00:00
abstract::Wilms' tumor development, like most tumors, involves multiple genetic alterations affecting diverse genes. Only one of these has thus far been identified, the Wilms' tumor 1 (WT1) gene on 11p13, which functions as a tumor suppressor gene. We assessed the involvement of the WT1 gene constitutionally and somatically in ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910630410
更新日期:1995-11-15 00:00:00
abstract::A gene product (p42) of the long open reading frame, now termed tax, of the viral genome of human T-cell leukemia virus type I (HTLV-I) may be related to the transformation of T cells in adult T-cell leukemia-lymphoma (ATLL). To evaluate its association with the disease, we compared the prevalence of antibody to p42 i...
journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:1989-06-15 00:00:00
abstract::China was one of the countries with the highest esophagus cancer risk in the world during the 1970s. This report provides data on time trends of esophagus cancer incidence and mortality during the 1970s-90s in selected geographic areas of China. Information on newly diagnosed cancer cases and cancer deaths is based on...
journal_title:International journal of cancer
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doi:10.1002/ijc.10706
更新日期:2002-11-20 00:00:00
abstract::Pseudomyxoma peritonei (PMP) is a rare disorder with unique pathological and genetic changes. Although several studies have reported the clinical features and mutational changes of PMP that originates from the appendix, few studies on PMP originating from the ovary have been reported due to its extreme rarity. In orde...
journal_title:International journal of cancer
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更新日期:2021-01-05 00:00:00