Gene expression changes during HPV-mediated carcinogenesis: a comparison between an in vitro cell model and cervical cancer.

Abstract:

:We used oligonucleotide microarrays to investigate gene expression changes associated with multi-step human papillomavirus type 16 (HPV16)-mediated carcinogenesis in vitro. Gene expression profiles in 4 early passage HPV16-immortalized human keratinocyte (HKc) lines derived from different donors were compared with their corresponding 4 late-passage, differentiation-resistant cell lines, and to 4 pools of normal HKc, each composed of 3 individual HKc strains, on Agilent 22 k human oligonucleotide microarrays. The resulting data were analyzed using a modified T-test coded in R to obtain lists of differentially expressed genes. Gene expression changes identified in this model system were then compared with gene expression changes described in published studies of cervical intraepithelial neoplasia (CIN) and cervical cancer. Common genes in these lists were further studied by cluster analysis. Genes whose expression changed in the same direction as in CIN or cervical cancer (concordant) at late stages of HPV16-mediated transformation in vitro formed one major cluster, while those that changed in the opposite direction (discordant) formed a second major cluster. Further annotation found that many discordant expression changes involved gene products with an extracellular localization. Two novel genes were selected for further study: overexpression of SIX1 and GDF15, observed during in vitro progression in our model system, was confirmed in tissue arrays of cervical cancer. These microarray-based studies show that our in vitro model system reflects many cellular and molecular alterations characteristic of cervical cancer, and identified SIX1 and GDF15 as 2 novel potential biomarkers of cervical cancer progression.

journal_name

Int J Cancer

authors

Wan F,Miao X,Quraishi I,Kennedy V,Creek KE,Pirisi L

doi

10.1002/ijc.23463

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

32-40

issue

1

eissn

0020-7136

issn

1097-0215

journal_volume

123

pub_type

杂志文章
  • Involvement of p53 in specific anti-neuroectodermal tumor activity of aloe-emodin.

    abstract::Previously, we have identified aloe-emodin (AE) as a new type of anticancer agent, with activity that is based on apoptotic cell death promoted by a neuroectodermal tumor-specific drug uptake. We attempt to clarify the intracellular target of AE and the apoptosis-signaling pathway activated by AE in neuroblastoma cell...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.11312

    authors: Pecere T,Sarinella F,Salata C,Gatto B,Bet A,Dalla Vecchia F,Diaspro A,Carli M,Palumbo M,Palù G

    更新日期:2003-10-10 00:00:00

  • Risk of invasive cervical cancer in relation to clinical investigation and treatment after abnormal cytology: a population-based case-control study.

    abstract::A substantial proportion of women with cervical cancer that have participated in cervical screening have a history of an abnormal cytology result. Our objective was to assess the impact of histological investigation and treatment of women with abnormal cytology on the subsequent risk of invasive cervical cancer. All i...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.25749

    authors: Silfverdal L,Kemetli L,Sparén P,Andrae B,Strander B,Ryd W,Dillner J,Törnberg S

    更新日期:2011-09-15 00:00:00

  • Identification of HLA class I dependent immunogenic peptides from clonotypic TCRbeta expressed in cutaneous T-cell lymphoma.

    abstract::The clonotypic T-cell receptor (TCR) is a potential target antigen for specific immunotherapy of cutaneous T-cell lymphoma (CTCL). We identified T-cell epitopes from the rearranged TCR beta chain of the malignant T-cell population by the "reverse immunology" approach. Peptide-specific T-cell lines were generated again...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.22113

    authors: Zeng J,Müller-Berghaus J,Nguyen XD,Klüter H,Schönhaber H,Song M,Schwinn N,Schadendorf D,Goerdt S,Eichmüller S,Dippel E

    更新日期:2006-11-15 00:00:00

  • Diverse expression patterns of the EMT suppressor grainyhead-like 2 (GRHL2) in normal and tumour tissues.

    abstract::The transcription factor grainyhead-like 2 (GRHL2) plays a crucial role in various developmental processes. Although GRHL2 recently has attracted considerable interest in that it could be identified as a novel suppressor of the epithelial-to-mesenchymal transition, evidence is emerging that GRHL2 also exhibits tumour-...

    journal_title:International journal of cancer

    pub_type: 杂志文章,meta分析

    doi:10.1002/ijc.29841

    authors: Riethdorf S,Frey S,Santjer S,Stoupiec M,Otto B,Riethdorf L,Koop C,Wilczak W,Simon R,Sauter G,Pantel K,Assmann V

    更新日期:2016-02-15 00:00:00

  • Smoking, snus use and risk of right- and left-sided colon, rectal and anal cancer: a 37-year follow-up study.

    abstract::Although some authorities consider smoking to be an established risk factor for colorectal cancer, the international literature is not entirely consistent. Further, only 1 study has addressed the association with smokeless tobacco and none with Scandinavian moist snuff (snus). This retrospective cohort study included ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.25305

    authors: Nordenvall C,Nilsson PJ,Ye W,Nyrén O

    更新日期:2011-01-01 00:00:00

  • Increased plasma DNA integrity index in head and neck cancer patients.

    abstract::Analysis of the length of circulating DNA in plasma has been reported as a marker for solid tumor detection. We assessed the sensitivity and specificity of increased plasma DNA length to identify patients with head and neck squamous cell carcinoma (HNSCC) and monitor posttreatment disease status. Fifty-eight HNSCC pat...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.22250

    authors: Jiang WW,Zahurak M,Goldenberg D,Milman Y,Park HL,Westra WH,Koch W,Sidransky D,Califano J

    更新日期:2006-12-01 00:00:00

  • Glucocorticoid-receptor-gene defects and resistance to glucocorticoid-induced apoptosis in human leukemic cell lines.

    abstract::The application of glucocorticoids (GC3) in human leukemia is based on apoptosis induction but is often hampered by GC resistance. To delineate resistance mechanisms, we examined 5 GC-resistant leukemic cell lines, termed CEM-C7.R1-R5, isolated from the GC-sensitive human acute-T-cell-leukemic line, CCRF-CEM-C7, by se...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0215(19961127)68:5<663::AID-IJC

    authors: Hala M,Hartmann BL,Böck G,Geley S,Kofler R

    更新日期:1996-11-27 00:00:00

  • Enhancement by TPA of phenotypes associated with transformation in carcinogen-treated human cells: evidence for a selective mechanism.

    abstract::The tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) was found to increase the incidence of phenotypic alterations induced by the direct-acting carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in human endometrial stromal cells. Following carcinogen treatment, changes in saturation density, gamma-glutam...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910320405

    authors: Siegfried JM,Kaufman DG

    更新日期:1983-10-15 00:00:00

  • Amplification of the EGF receptor and c-myc genes in human esophageal cancers.

    abstract::The incidence of esophageal cancer is extremely high in Linxian County and certain other regions of the People's Republic of China. Epidemiologic and laboratory studies suggest that N-nitroso carcinogens and other environmental factors play a causative role. In the present study, employing over 100 DNA samples obtaine...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910420406

    authors: Lu SH,Hsieh LL,Luo FC,Weinstein IB

    更新日期:1988-10-15 00:00:00

  • Incidence and mortality from stomach cancer in Japan, Slovenia and the USA.

    abstract::The mortality and incidence from stomach cancer were compared in Japan (a country with a high incidence where there was full application of mass screening during this period) and 2 countries with no screening policy: the USA (with a very low incidence) and Slovenia (with an intermediate rate). The registered cases of ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.10150

    authors: Lambert R,Guilloux A,Oshima A,Pompe-Kirn V,Bray F,Parkin M,Ajiki W,Tsukuma H

    更新日期:2002-02-20 00:00:00

  • Prosurvival function of the granulin-epithelin precursor is important in tumor progression and chemoresponse.

    abstract::The granulin-epithelin precursor (GEP/PCDGF), a 68-88 kDa secreted glycoprotein, has been shown to be an important growth and survival factor for ovarian cancer cells. Furthermore, GEP expression is a predictor of patient survival in metastatic ovarian cancer cells. Up to this point, however, the molecular mechanisms ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.22559

    authors: Pizarro GO,Zhou XC,Koch A,Gharib M,Raval S,Bible K,Jones MB

    更新日期:2007-06-01 00:00:00

  • Focal amplifications are associated with high grade and recurrences in stage Ta bladder carcinoma.

    abstract::Urinary bladder cancer is a heterogeneous disease with tumors ranging from papillary noninvasive (stage Ta) to solid muscle infiltrating tumors (stage T2+). The risk of progression and death for the most frequent diagnosed type, Ta, is low, but the high incidence of recurrences has a significant effect on the patients...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.24954

    authors: Nord H,Segersten U,Sandgren J,Wester K,Busch C,Menzel U,Komorowski J,Dumanski JP,Malmström PU,Díaz de Ståhl T

    更新日期:2010-03-15 00:00:00

  • Receptors for hormones and growth factors and (onco)-gene amplification in human ovarian cancer.

    abstract::Receptor status and gene amplification were studied in advanced human ovarian adenocarcinoma tissues, borderline and benign ovarian tumours and normal ovarian tissues. Sixty-five percent (53/82) of ovarian adenocarcinomas, 57% (8/14) of benign/borderline tumours and only 31% (5/16) of normal ovarian tissues studied sh...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910520211

    authors: Berns EM,Klijn JG,Henzen-Logmans SC,Rodenburg CJ,van der Burg ME,Foekens JA

    更新日期:1992-09-09 00:00:00

  • Tumor-associated macrophage-targeted therapeutics in ovarian cancer.

    abstract::Ovarian cancer is one of the most common gynecological malignancies. The tumor microenvironment plays an important role in regulating the progression of ovarian cancer. Macrophages, which are important immune cells in the tumor microenvironment, participate in the regulation of various biological behaviors and influen...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审

    doi:10.1002/ijc.33408

    authors: An Y,Yang Q

    更新日期:2020-11-24 00:00:00

  • Lymphangiogenesis in colorectal cancer--prognostic and therapeutic aspects.

    abstract::Colorectal cancer (CRC) represents the second most common cause of cancer mortality in the western world. The tumors frequently show metastatic spread which affects different organs such as lymph nodes, liver and lungs. Although the pattern of spread may vary, the initial step usually involves dissemination to regiona...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审

    doi:10.1002/ijc.22996

    authors: Sundlisaeter E,Dicko A,Sakariassen PØ,Sondenaa K,Enger PØ,Bjerkvig R

    更新日期:2007-10-01 00:00:00

  • Establishment of primary cultures from human colonic tissue during tumor progression: vitamin-D responses and vitamin-D-receptor expression.

    abstract::Primary cultures derived from pre-cancerous and cancerous human colon tissue are essential for understanding normal and abnormal growth function in the large intestine. Here presented are (i) the methodology for routine establishment of primary cultures of normal, adenoma- and carcinoma-derived cells, and (ii) data fo...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(19980130)75:3<467::aid-ijc

    authors: Tong WM,Bises G,Sheinin Y,Ellinger A,Genser D,Pötzi R,Wrba F,Wenzl E,Roka R,Neuhold N,Peterlik M,Cross HS

    更新日期:1998-01-30 00:00:00

  • Ki-67 index enhances the prognostic accuracy of the urothelial superficial bladder carcinoma risk group classification.

    abstract::Approximately 80% of bladder tumors are urothelial superficial papillary carcinomas (USPC). Despite a generally good prognosis, these tumors have a strong propensity to recur and about 1/3 of them compared to disease progression. Histological assessment of these superficial tumors is not sufficiently discriminator in ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.11049

    authors: Santos L,Amaro T,Costa C,Pereira S,Bento MJ,Lopes P,Oliveira J,Criado B,Lopes C

    更新日期:2003-06-10 00:00:00

  • Alterations of 3p21.31 tumor suppressor genes in head and neck squamous cell carcinoma: Correlation with progression and prognosis.

    abstract::The aim of our study was to analyze the alterations of some candidate tumor suppressor genes (TSGs) viz. LIMD1, LTF, CDC25A, SCOTIN, RASSF1A and CACNA2D2 located in the chromosomal region 3p21.31 associated with the development of early dysplastic lesions of head and neck. In analysis of 72 dysplastic lesions and 116 ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.23834

    authors: Ghosh S,Ghosh A,Maiti GP,Alam N,Roy A,Roy B,Roychoudhury S,Panda CK

    更新日期:2008-12-01 00:00:00

  • Upregulation of matrix metalloproteinase-9 in murine 5T33 multiple myeloma cells by interaction with bone marrow endothelial cells.

    abstract::MM is a B-cell malignancy mainly characterized by monoclonal expansion of plasma cells in the BM, presence of paraprotein in serum and occurrence of osteolytic bone lesions. MMPs are a family of proteolytic enzymes that can contribute to cancer growth, invasion, angiogenesis, bone degradation and other processes impor...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.10642

    authors: Van Valckenborgh E,Bakkus M,Munaut C,Noël A,St Pierre Y,Asosingh K,Van Riet I,Van Camp B,Vanderkerken K

    更新日期:2002-10-20 00:00:00

  • Androgen receptor-interacting protein HSPBAP1 facilitates growth of prostate cancer cells in androgen-deficient conditions.

    abstract::Hormonal therapies targeting androgen receptor (AR) are effective in prostate cancer (PCa), but often the cancers progress to fatal castrate-resistant disease. Improved understanding of the cellular events during androgen deprivation would help to identify survival and stress pathways whose inhibition could synergize ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.29303

    authors: Saeed K,Östling P,Björkman M,Mirtti T,Alanen K,Vesterinen T,Sankila A,Lundin J,Lundin M,Rannikko A,Nordling S,Mpindi JP,Kohonen P,Iljin K,Kallioniemi O,Rantala JK

    更新日期:2015-06-01 00:00:00

  • Bioactive tumour necrosis factor alpha but not granulocyte-macrophage colony-stimulating factor correlates inversely with Langerhans's cell numbers in skin tumours.

    abstract::Langerhans' cells (LCs) are thought to play an important role in presentation of tumour antigens for the induction of anti-tumour immunity. Epidermis overlying some transplanted murine skin tumours contains increased numbers of LCs; however, alterations in LC numbers are not related to tumour antigenicity or host immu...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(19980119)75:2<210::aid-ijc

    authors: Rubel DM,Barnetson RS,Halliday GM

    更新日期:1998-01-19 00:00:00

  • Requirement for the continual presence of lipopolysaccharide for production of tumor necrosis factor by thioglycollate-induced peritoneal murine macrophages.

    abstract::This work demonstrates the need for the continued presence of lipopolysaccharide (LPS) for tumor necrosis factor (TNF) production by thioglycollate-induced peritoneal macrophages. Removal of LPS at any time resulted in the abrupt cessation of further TNF production. The readdition of LPS resulted in further production...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910380121

    authors: Gifford GE,Lohmann-Matthes ML

    更新日期:1986-07-15 00:00:00

  • Small interfering RNA-mediated CXCR1 or CXCR2 knock-down inhibits melanoma tumor growth and invasion.

    abstract::CXCR1 and CXCR2 are receptors for CXCL-8 and are differentially expressed on melanoma and endothelial cells. In this study, we determined the functional role of these receptors in melanoma progression. We stably knock-down the expression of CXCR1 and/or CXCR2 in A375-SM (SM; high metastatic) human melanoma cells by sh...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.24714

    authors: Singh S,Sadanandam A,Varney ML,Nannuru KC,Singh RK

    更新日期:2010-01-15 00:00:00

  • Predictors of level of circulating abnormal lymphocytes among human T-lymphotropic virus type I carriers in Japan.

    abstract::Human T-lymphotropic virus type I (HTLV-I) carriers often have abnormal lymphocytes (Ably) that resemble malignant cells of adult T-cell leukemia (ATL). To identify predictors of the level of Ably in a longitudinal study of asymptomatic HTLV-I carriers, we analyzed data from 215 subjects (67 men and 148 women) with mu...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(19980717)77:2<188::aid-ijc

    authors: Hisada M,Okayama A,Tachibana N,Stuver SO,Spiegelman DL,Tsubouchi H,Mueller NE

    更新日期:1998-07-17 00:00:00

  • Estimation of the effects of smoking and DNA repair capacity on coefficients of a carcinogenesis model for lung cancer.

    abstract::Numerous prospective and retrospective studies have clearly demonstrated a dose-related increased lung cancer risk associated with cigarette smoking, with evidence also for a genetic component to risk. In this study, using the two-stage clonal expansion stochastic model framework, for the first time we investigated th...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.24149

    authors: Deng L,Kimmel M,Foy M,Spitz M,Wei Q,Gorlova O

    更新日期:2009-05-01 00:00:00

  • BCAR1/p130Cas expression in untreated and acquired tamoxifen-resistant human breast carcinomas.

    abstract::High BCAR1/p130Cas expression in primary breast tumour cytosol predicts a poor chance of response recurrent disease to tamoxifen treatment in patients with oestrogen receptor (ER)-positive breast carcinomas. In this study, we assessed whether BCAR1/p130Cas expression is altered during acquisition of anti-oestrogen res...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/1097-0215(20000920)89:5<465::aid-ijc11>3.0

    authors: van der Flier S,Chan CM,Brinkman A,Smid M,Johnston SR,Dorssers LC,Dowsett M

    更新日期:2000-09-20 00:00:00

  • Molecular variants of HPV-16 associated with cervical cancer in Indian population.

    abstract::Human papilloma virus is a causative factor in the etiology of cervical cancer with HPV16 being the most prevalent genotype associated with it. Intratype variations in oncogenic E6/E7 and capsid L1 proteins of HPV 16 besides being of phylogenetic importance, are associated with risk of viral persistence and progressio...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究

    doi:10.1002/ijc.24322

    authors: Pillai MR,Hariharan R,Babu JM,Lakshmi S,Chiplunkar SV,Patkar M,Tongaonkar H,Dinshaw K,Jayshree RS,Reddy BK,Siddiqui M,Roychoudury S,Saha B,Abraham P,Gnanamony M,Peedicayil A,Subhashini J,Ram TS,Dey B,Sharma C,Jain

    更新日期:2009-07-01 00:00:00

  • A distinct DNA methylation profile associated with microsatellite and chromosomal stable sporadic colorectal cancers.

    abstract::Aberrant DNA methylation, microsatellite instability (MSI) and chromosomal instability (CIN) are well-characterised molecular features of sporadic colorectal cancers (CRCs). In addition to CpG island methylator phenotype (CIMP) associated with MSI, an intermediate methylation subgroup is also a feature of non-MSI canc...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.26104

    authors: Silver A,Sengupta N,Propper D,Wilson P,Hagemann T,Patel A,Parker A,Ghosh A,Feakins R,Dorudi S,Suraweera N

    更新日期:2012-03-01 00:00:00

  • AFP-synthesis induction in mouse hepatomas.

    abstract::Highly differentiated, spontaneous hepatomas occurring in 13- to 15-month-old CBA mice were unable to produce embryo-specific serum protein alpha-fetoprotein (AFP). AFP synthesis, however, was induced in such tumors by minimal mechanical damage (small incisions of the tumors). On the 3rd day following surgery, AFP-pos...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910350121

    authors: Shipova LY,Gleiberman AS

    更新日期:1985-01-15 00:00:00

  • Carcinogenic potential of hycanthone in mice and hamsters.

    abstract::Hycanthone was administered to Schistosoma mansoni-infected and non-infected Syrian golden hamsters and Swiss mice by intraperitoneal and intramuscular injection of amounts up to the maximum tolerated dose. No tumors attributable to treatment were observed in hamsters. In infected mice, the overall incidence of hepato...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910230117

    authors: Bulay O,Urman H,Patil K,Clayson DB,Shubik P

    更新日期:1979-01-15 00:00:00