Human ZNF312b oncogene is regulated by Sp1 binding to its promoter region through DNA demethylation and histone acetylation in gastric cancer.


:In a previous study, human ZNF312b was identified as a cell proliferation-associated oncogene via the K-ras/extracellular signal-regulated kinase cascade in gastric cancer. However, the mechanism concerning its transcriptional activation remains unknown. Here, we show that DNA methylation and histone acetylation of the ZNF312b promoter function as a switch for ZNF312b transcriptional activation in gastric cancer. The transcription level of ZNF312b was increased by treatment with a demethylating agent, 5-aza-2'-deoxycytidine and the histone deacetylase inhibitor sodium butyrate, in several human cancer cell lines including gastric cancer. Consistent with these results, epigenetic analysis, such as pyrosequencing, bisulfate sequencing and methyl-specific polymerase chain reaction (MSP), showed that the expression level of ZNF312b is highly dependent on the degree of DNA methylation in gastric cancer cell lines. In addition, by ChIP assay using anti-acetyl/methyl H3K9 antibodies, histone acetylation was shown to mediate the expression of the ZNF312b gene. Interestingly, ChIP assay using the Sp1 antibody revealed that the binding of transcription factor Sp1 to the ZNF312b promoter for its transcriptional activation requires DNA demethylation and histone acetylation. Moreover, a knockdown of Sp1 resulted in a decrease in ERK-mediated proliferation via downregulation of the ZNF312b gene in gastric cancer cells. Taken together, these results suggest that the aberrant expression of ZNF312b promotes gastric tumorigenesis through epigenetic modification of its promoter region and provides a molecular mechanism for ZNF312b expression to contribute to the progression of gastric cancer.


Int J Cancer


Song IS,Ha GH,Kim JM,Jeong SY,Lee HC,Kim YS,Kim YJ,Kwon TK,Kim NS




Has Abstract


2011-11-01 00:00:00












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    abstract::A population-based case-control study of esophageal cancer was conducted in urban Shanghai involving interviews with 902 cases and 1,552 controls. Risk of esophageal cancer was increased among tobacco smokers and alcohol drinkers. Odds ratios (OR) for smoking were 2.1 and 1.6 for men and women, respectively, and incre...

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    pub_type: 杂志文章


    authors: Gao YT,McLaughlin JK,Blot WJ,Ji BT,Benichou J,Dai Q,Fraumeni JF Jr

    更新日期:1994-07-15 00:00:00

  • Chromosome 3p24-26 and 3p22-12 loss in human prostatic adenocarcinoma.

    abstract::Identification of loss of heterozygosity on specific genetic loci is crucial for understanding the pathogenesis of prostate cancer at the molecular level. This is especially important because the deleted regions may contain putative tumor suppressor genes. Chromosome 3p loss appears to be frequently associated with va...

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    pub_type: 杂志文章


    authors: Dahiya R,McCarville J,Hu W,Lee C,Chui RM,Kaur G,Deng G

    更新日期:1997-03-28 00:00:00

  • Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization.

    abstract::A panel of monoclonal antibodies (MAbs) has been obtained, directed against determinants of normal human colorectal epithelium. BALB/c mice were immunized and boosted with mucosal scrapings and cell membrane preparations from normal large intestine. In one case boosting was also performed with HT29 colon carcinoma cel...

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    pub_type: 杂志文章


    authors: Richman PI,Bodmer WF

    更新日期:1987-03-15 00:00:00

  • Leukaemias in BN/a and BN/b mice after prolonged treatment with antilymphocyte globulin.

    abstract::The influence of prolonged treatment with antilymphocyte globulin on tumour incidence in BN/a and BN/b mice was studied. One ALG sample appeared to be highly leukaemogenic in two independent experiments. All treated mice developed, after a short latency period, non-thymic lymphomas with involvement of abdominal lymph ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Szkudlarek J,Radzikowski C,Czarnomska A,Dux K,Bartoszewicz W

    更新日期:1976-12-15 00:00:00

  • Ganglioside GM2 on the K562 cell line is recognized as a target structure by human natural killer cells.

    abstract::Although human NK cells lyse a wide spectrum of target cells, the precise target structure recognized by NK cells has not yet been elucidated. In order to define a possible relationship between gangliosides on human target cells and susceptibility to NK lysis, 14 human leukemia and lymphoma cell lines were studied. A ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ando I,Hoon DS,Suzuki Y,Saxton RE,Golub SH,Irie RF

    更新日期:1987-07-15 00:00:00

  • Telomerase reverse transcriptase mutations are independent predictor of disease-free survival in Middle Eastern papillary thyroid cancer.

    abstract::Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Tumor recurrence occurs in ∼20% of PTCs and some reach advanced stages. Promoter mutation in the telomerase reverse transcriptase (TERT) gene is identified to be a prognostic marker in PTC. However, the contribution of TERT promoter mutation ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bu R,Siraj AK,Divya SP,Kong Y,Parvathareddy SK,Al-Rasheed M,Al-Obaisi KAS,Victoria IG,Al-Sobhi SS,Al-Dawish M,Al-Dayel F,Al-Kuraya KS

    更新日期:2018-05-15 00:00:00

  • Selective inhibition of PED protein expression sensitizes B-cell chronic lymphocytic leukaemia cells to TRAIL-induced apoptosis.

    abstract::B-cell chronic lymphocytic leukaemia (B-CLL) cells fail to undergo apoptosis. The mechanism underlying this resistance to cell death is still largely unknown. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) effectively kills tumour cells but not normal cells, and thus represents an attractive tool for...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Garofalo M,Romano G,Quintavalle C,Romano MF,Chiurazzi F,Zanca C,Condorelli G

    更新日期:2007-03-15 00:00:00

  • The initiation of tumours on mouse skin by dihydrodiols derived from 7,12-dimethylbenz(a)anthracene and 3-methylcholanthrene.

    abstract::The cis-2a,3-diol and the trans-4,5-, trans-7,8-, trans-9,10- and trans-11,12-dihydrodiols of 3-methylcholanthrene and the trans-3,4, trans-5,6-, trans-8,9. and trans-10,11- dihydrodiols of 7,12-dimethylbenz[a]anthrancene have been tested, in comparison with the parent hydrocarbons, for their abilities to initiate sk...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Chouroulinkov I,Gentil A,Tierney B,Grover PL,Sims P

    更新日期:1979-10-15 00:00:00

  • Combined effects of natural resistance and chemotherapy against a murine lymphoma in lethally-irradiated mice.

    abstract::Lymphoma EL-4 of B6 origin was inoculated into lethally-irradiated syngeneic B6 or resistant BD2F1 hybrid recipients. Marked impairment of lymphoma cell proliferation (i.e. hybrid resistance, HR) occurred in the spleen but not in the liver or lung of BD2F1 hosts. Treatment with DTIC at the optimal dose of 80 mg/kg i.p...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Iorio AM,Neri M,Enrico P,Goldin A,Bonmassar E

    更新日期:1981-10-15 00:00:00

  • MCC inhibits beta-catenin transcriptional activity by sequestering DBC1 in the cytoplasm.

    abstract::The mutated in colorectal cancer (MCC) is a multifunctional gene showing loss of expression in colorectal and liver cancers. MCC mutations can drive colon carcinogenesis in the mouse and in vitro experiments suggest that loss of MCC function promotes cancer through several important cellular pathways. In particular, t...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pangon L,Mladenova D,Watkins L,Van Kralingen C,Currey N,Al-Sohaily S,Lecine P,Borg JP,Kohonen-Corish MR

    更新日期:2015-01-01 00:00:00

  • The ability of normal mouse cells to reduce the malignant potential of transformed mouse bladder epithelial cells depends on their somatic origin.

    abstract::Somatic cell hybrids have been made between a transformed mouse bladder carcinoma cell line and normal mouse bladder epithelium and mesenchyme. In the epithelial tumour/mesenchyme hybrids the malignant phenotype was expressed dominantly whereas in the carcinoma/normal epithelium hybrids the malignant potential was gre...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cowell JK,Franks LM

    更新日期:1984-05-15 00:00:00

  • Dysregulated expression of transforming growth factor beta and its type-I and type-II receptors in basal-cell carcinoma.

    abstract::In mammals, transforming growth factor-beta (TGF-beta) is found in 3 highly homologous isoforms that exert their effects via heteromeric complexes of type-I and type-II receptors (TbetaR-I and TbetaR-II). TGF-beta regulates the growth and metabolism of various cell types, including keratinocytes. We have investigated ...

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    pub_type: 杂志文章


    authors: Furue M,Kato M,Nakamura K,Nashiro K,Kikuchi K,Okochi H,Miyazono K,Tamaki K

    更新日期:1997-05-16 00:00:00

  • Tamoxifen and toremifene treatment of breast cancer and risk of subsequent endometrial cancer: a population-based case-control study.

    abstract::A population-based case-control study was performed to evaluate the risk of endometrial cancer related to tamoxifen or toremifene treatment. All patients with breast cancer diagnosis since 1980 in Finland who subsequently developed an endometrial cancer by the end of 1995 and 3 matched controls were identified among t...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pukkala E,Kyyrönen P,Sankila R,Holli K

    更新日期:2002-07-20 00:00:00

  • Enhanced antitumor efficacy of telomerase-selective oncolytic adenoviral agent OBP-401 with docetaxel: preclinical evaluation of chemovirotherapy.

    abstract::Oncolytic adenoviruses are being developed as novel anticancer therapeutics and currently undergoing clinical trials. We previously demonstrated that telomerase-specific replication-competent adenovirus (Telomelysin: OBP-301), in which the human telomerase reverse transcriptase (hTERT) promoter regulates viral replica...

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    pub_type: 杂志文章


    authors: Fujiwara T,Kagawa S,Kishimoto H,Endo Y,Hioki M,Ikeda Y,Sakai R,Urata Y,Tanaka N,Fujiwara T

    更新日期:2006-07-15 00:00:00

  • Humanization and characterization of the anti-HLA-DR antibody 1D10.

    abstract::1D10 is a previously described antibody that binds to cells from a majority of B-cell malignancies. The current studies were designed to further evaluate the antigen specificity of 1D10 and its potential as an immunotherapeutic agent. Studies with transfectants and immunoprecipitation demonstrated that 1D10 recognizes...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kostelny SA,Link BK,Tso JY,Vasquez M,Jorgensen BH,Wang H,Hall WC,Weiner GJ

    更新日期:2001-08-15 00:00:00

  • Human papillomavirus capsids preferentially bind and infect tumor cells.

    abstract::We previously determined that human papillomavirus (HPV) virus-like particles (VLPs) and pseudovirions (PsV) did not, respectively, bind to or infect intact epithelium of the cervicovaginal tract. However, they strongly bound heparan sulfate proteoglycans (HSPG) on the basement membrane of disrupted epithelium and inf...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kines RC,Cerio RJ,Roberts JN,Thompson CD,de Los Pinos E,Lowy DR,Schiller JT

    更新日期:2016-02-15 00:00:00

  • Subtypes of Epstein-Barr virus in HIV-1-associated and HIV-1-unrelated Hodgkin's disease cases.

    abstract::Epstein-Barr virus (EBV) type 2 is considered to be a much less potent transformer of lymphocytes than type 1. However, type-2 EBV may be involved in the pathogenesis of non-Hodgkin's lymphomas (NHLs) arising in immunocompromised patients, i.e., subjects with malaria or HIV-1 infection. To determine whether type-2 EBV...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: De Re V,Boiocchi M,De Vita S,Dolcetti R,Gloghini A,Uccini S,Baroni C,Scarpa A,Cattoretti G,Carbone A

    更新日期:1993-07-30 00:00:00

  • Association between circulating concentrations of 25(OH)D and colorectal adenoma: a pooled analysis.

    abstract::The relationship between the biomarker of vitamin D status, 25(OH)D, and the risk for colorectal neoplasia is suggestive but equivocal. Questions remain regarding whether there are differential associations between 25(OH)D and colorectal adenoma by gender, colorectal subsite or features of baseline and recurrent adeno...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Jacobs ET,Hibler EA,Lance P,Sardo CL,Jurutka PW

    更新日期:2013-12-15 00:00:00

  • A prospective, population-based study of 40,000 women regarding host factors, UV exposure and sunbed use in relation to risk and anatomic site of cutaneous melanoma.

    abstract::Prospective cohort studies about cutaneous melanoma (CM) risk are still few. Host factor- and UVR exposure data were collected prospectively by questionnaire in this population-based cohort study including 40,000 Swedish born women, aged 25-64 years at enrolment (1990). Risk for CM (Cox regression and Stepwise Cox reg...

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    pub_type: 杂志文章


    authors: Nielsen K,Måsbäck A,Olsson H,Ingvar C

    更新日期:2012-08-01 00:00:00

  • Novel protein kinases expressed in human breast cancer.

    abstract::The family of protein kinases includes many oncogenes and growth-factor receptors, as well as genes that are involved in cell-cycle regulation. We have identified protein kinases expressed in a human breast-cancer cell line, 600PEI, and a primary human breast carcinoma, using PCR cloning techniques based on consensus ...

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    authors: Cance WG,Craven RJ,Weiner TM,Liu ET

    更新日期:1993-06-19 00:00:00

  • Association of two mutations in the CHEK2 gene with breast cancer.

    abstract::The 1100delC mutation of the cell cycle checkpoint kinase 2 (CHEK2) gene confers an increased risk for breast cancer, but the clinical impact of other CHEK2 gene variants remains unclear. We determined the frequency of two functionally relevant CHEK2 gene mutations, I157T and IVS2+1G > A, in two large series of breast...

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    authors: Bogdanova N,Enssen-Dubrowinskaja N,Feshchenko S,Lazjuk GI,Rogov YI,Dammann O,Bremer M,Karstens JH,Sohn C,Dörk T

    更新日期:2005-08-20 00:00:00

  • Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus.

    abstract::Fischer rat thyroid epithelial cella (FRT) growing continuously in culture were infected with the Kirsten murine sarcoma virus KiMSV(KiMuLV) and found to produce this virus constitutively. Although the morphology of the FRT cells did not change appreciably, the cells became malignant after infection with KiMSV(KiMuLV)...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Fusco A,Pinto A,Ambesi-Impiombato FS,Vecchio G,Tsuchida N

    更新日期:1981-11-15 00:00:00

  • The association between markers of tumour cell metabolism, the tumour microenvironment and outcomes in patients with colorectal cancer.

    abstract::Tumour cell anaerobic metabolism has been reported to be a prognostic factor in colorectal cancer. The present study investigated the association between monocarboxylate transporter (MCT) 1, MCT 2, lactate dehydrogenase (LDH) 1 and LDH 5, the tumour microenvironment, and outcome in patients with colorectal cancer. A c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Roseweir AK,Clark J,McSorley ST,vanWyk HC,Quinn JA,Horgan PG,McMillan DC,Park JH,Edwards J

    更新日期:2019-05-01 00:00:00

  • Autoimmune expression of a cytoplasmic protein p60 in mice bearing metastasizing SV40-transformed tumors.

    abstract::In STU mice bearing metastasizing SV40-transformed 51A-232B-M tumors, an immune response against a cellular 60kDa protein (p60) developed in about 50% of the tumor-bearing animals, in addition to the response against SV40 large T-antigen and cellular protein p53. The anti-p60 auto-immune response could be observed as ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Manneck HE,Pfaff M,Anderer FA

    更新日期:1988-12-15 00:00:00

  • Tumor necrosis factor alpha induces early morphologic and metabolic alterations in Friend leukemia cell tumors and fibrosarcomas in mice.

    abstract::Morphologic and metabolic studies have been carried out on Friend leukemia cell (FLC) tumors (grown in DBA/2 mice) or fibrosarcomas (grown in C3H/HeN or C3H/HeJ mice) shortly after peritumoral injection of recombinant mouse tumor necrosis factor (TNF) alpha. Marked vascular congestion and focal extravasation of erythr...

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    pub_type: 杂志文章


    authors: Proietti E,Belardelli F,Carpinelli G,Di Vito M,Woodrow D,Moss J,Sestili P,Fiers W,Gresser I,Podo F

    更新日期:1988-10-15 00:00:00

  • Circadian nutritional behaviours and cancer risk: New insights from the NutriNet-santé prospective cohort study: Disclaimers.

    abstract::Circadian disruption has been classified as probably carcinogenic to humans by the World Health Organization's International Agency for Research on Cancer. The circadian clock is subject to environmental factors, particularly light exposure and food intake rhythms. However, the association between nutritional circadia...

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    authors: Srour B,Plancoulaine S,Andreeva VA,Fassier P,Julia C,Galan P,Hercberg S,Deschasaux M,Latino-Martel P,Touvier M

    更新日期:2018-11-15 00:00:00

  • Interferon-alpha/beta in virus-induced mouse mammary carcinogenesis: effects on the spontaneous process and on the progression of transplanted pre-neoplastic lesions.

    abstract::Low levels of anti-viral activity, mainly interferon alpha/beta (IFN-alpha/beta), are regularly found in lymphoid tissues of BALB/c mice infected with the C3H strain of mammary tumor virus. At the time of tumor development, significant amounts of anti-viral activity were detected in homogenates of spleen and mammary t...

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    pub_type: 杂志文章


    authors: Basolo F,Fontanini G,Serra C,Dolei A,Proietti E,Belardelli F,Conaldi PG,Bistocchi M,Squartini F,Toniolo A

    更新日期:1992-07-30 00:00:00

  • Antigen shedding vs. development of natural suppressor cells as mechanism of tumor escape in mice bearing Ehrlich tumor.

    abstract::C57BL/6J mice immunized with devitalized Ehrlich tumor (ET) cells produce high serum levels of IgM antibodies to ET cell-surface carbohydrates that are critical in the observed resistance against this tumor. However, this response is not found in ET-bearing mice at any stage of tumor development. Since previous studie...

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    pub_type: 杂志文章


    authors: Viñuela JE,Rodriguez R,Gil J,Coll J,De la Concha EG,Subiza JL

    更新日期:1991-01-02 00:00:00

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    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Parkin DM,Ferlay J,Curado MP,Bray F,Edwards B,Shin HR,Forman D

    更新日期:2010-12-15 00:00:00

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    abstract::The autophagy lysosome pathway is essential to maintain cell viability and homeostasis in response to many stressful environments, which is reported to play a vital role in cancer development and therapy. However, the association of genetic alterations of this pathway with risk of cancer remains unclear. Based on geno...

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    authors: Tan J,Fu L,Chen H,Guan J,Chen Y,Fang J

    更新日期:2018-07-01 00:00:00