Abstract：
:Our aim was to analyze the expression of the serine protease HtrA1 in human bladder tissue and urine in order to point out its possible association with the presence of urothelial bladder cancer. Bladder tissue and urine specimens from cancer patients with different tumor grades and stages (n = 68) and from individuals with cystitis (n = 16) were collected along with biopsy specimens and urine from healthy individuals (n = 68). For the first time, we demonstrated by immunohistochemistry that HtrA1 protein is produced by bladder urothelium in both physiological and inflammatory conditions, whereas it is not detectable in urothelial cancer cells regardless of tumor grade and stage. A different HtrA1 expression between normal-looking and neoplastic bladder tissue, despite similar HtrA1 mRNA levels, was also found by western blotting, which disclosed the presence of two forms of HtrA1, a native form of ∼50 kDa and an autocatalytic form of ∼38 kDa. Our investigations documented the presence of the two forms of HtrA1 also in urine. The ∼38 kDa form was significantly down-regulated in neoplastic tissue, whereas significantly higher amounts of both HtrA1 forms were found in urine from cancer patients compared with both healthy subjects and patients with cystitis. Our findings suggest that HtrA1 is a downexpressed molecule since an early stage of bladder urothelial carcinoma development and that urinary HtrA1 protein may be considered, if successfully validated, as an early and highly sensitive and specific biomarker for this neoplasia (the sensitivity and specificity of HtrA1 are 92.65% and 95.59%, respectively).
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Lorenzi T,Lorenzi M,Altobelli E,Marzioni D,Mensà E,Quaranta A,Paolinelli F,Morroni M,Mazzucchelli R,De Luca A,Procopio AD,Baldi A,Muzzonigro G,Montironi R,Castellucci Mdoi
10.1002/ijc.28280subject
Has Abstractpub_date
2013-12-01 00:00:00pages
2650-61issue
11eissn
0020-7136issn
1097-0215journal_volume
133pub_type
杂志文章abstract：:Low levels of anti-viral activity, mainly interferon alpha/beta (IFN-alpha/beta), are regularly found in lymphoid tissues of BALB/c mice infected with the C3H strain of mammary tumor virus. At the time of tumor development, significant amounts of anti-viral activity were detected in homogenates of spleen and mammary t...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910510620
更新日期：1992-07-30 00:00:00
abstract：:A segment of 712 bases coding for part of the human stearoyl-CoA desaturase gene was made by polymerase chain reaction (PCR) using primers based on published rat cDNA sequences. The human PCR product was confirmed by DNA sequencing. It was next cloned into a vector from which anti-sense, highly radioactive RNA transcr...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910570310
更新日期：1994-05-01 00:00:00
abstract：:Two HLA-A2 restricted epitopes have recently been identified from the broadly expressed tumor antigen survivin, and several vaccination trials in cancer patients based on these survivin-derived peptides have been initiated. Consequently, there is a crucial need for the identification of survivin epitopes restricted to...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.11634
更新日期：2004-03-01 00:00:00
abstract：:Fibrin deposition in the microenvironment of tumor cells may be critical to tumor growth and metastasis formation. Procoagulant activities of tumor cells themselves, or of infiltrating macrophages, activated by the host's immune system to the tumor, may both contribute to coagulopathies associated with metastases. Lym...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910370506
更新日期：1986-05-15 00:00:00
abstract：:There is a great need to identify new and better prognostic and predictive biomarkers to stratify prostate cancer patients for optimal treatment. The aims of this study were to characterize the expression profile of pre-B cell leukemia homeobox (PBX) transcription factors in prostate cancer with an emphasis on investi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.30220
更新日期：2016-10-15 00:00:00
abstract：:Hyaluronan (HA) has been shown to play crucial roles in the tumorigenicity of malignant tumors. Previous studies demonstrated that inhibition of HA suppressed the tumorigenicity of various malignant tumors including breast cancer. 4-methylumbelliferone (MU) has been reported to inhibit HA synthesis in several cell typ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.26014
更新日期：2012-01-15 00:00:00
abstract：:Within the Netherlands Cohort Study on diet and cancer, we investigated associations between total alcohol consumption, specific alcoholic beverage consumption and risk of colorectal cancer (CRC) according to anatomical subsite. Hazard Ratios (HR) and 95% confidence intervals (CI) were estimated using Cox proportional...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23774
更新日期：2008-11-15 00:00:00
abstract：:Recently reported genome-wide association studies have identified more than 20 common low-penetrance colorectal cancer (CRC) susceptibility loci. Recent studies have reported that copy number variations (CNVs) are considered important human genomic variants related to cancer, while the contribution of CNVs remains unc...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.30421
更新日期：2017-01-01 00:00:00
abstract：:Mammalian LIM kinase 1 (LIMK1) phosphorylates and inactivates the actin-binding and -depolymerizing factor cofilin and induces actin cytoskeletal changes. LIMK1 is reported to play an important role in cell motility, but the mechanism of induction of cell motility and the role of LIMK1 in tumor growth, angiogenesis an...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.21650
更新日期：2006-06-01 00:00:00
abstract：:In the present study we examined the expression of concanavalin-A(Con-A)-like molecules on natural-killer (NK)-sensitive target cells and investigated their possible role in the human NK-cell phenomenon. The incubation of either peripheral-blood lymphocytes (PBL) or large granular lymphocytes (LGL) with swainsonine (S...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910520428
更新日期：1992-10-21 00:00:00
abstract：:Dendritic cells (DCs) and natural killer (NK) cells are key players at the interface between innate resistance and acquired immunity. NK cells can induce DC maturation, a differentiation process whereby DCs respond to a environmental stimulus and acquire the ability of eliciting adaptive immunity. Conversely, maturing...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22279
更新日期：2006-12-15 00:00:00
abstract：:Cancers occurring at the gastroesophageal junction (GEJ) are classified as predominantly esophageal or gastric, which is often difficult to decipher. We hypothesized that the transcriptomic profile might reveal molecular subgroups which could help to define the tumor origin and behavior beyond anatomical location. The...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32384
更新日期：2019-12-15 00:00:00
abstract：:The role of natural immune defense in the control of metastatic spread of tumor cells was evaluated by adapting an s.c.-grown, antigenic, but non-immunogenic rat fibrosarcoma (Bsp6S) to ascitic growth (BSp6A) in the strain of origin (BDX), where peritoneal cells display a high level of NK and macrophage activity. Para...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910370119
更新日期：1986-01-15 00:00:00
abstract：:We performed microsatellite analysis and tested telomerase activity in paired tissue and urine of bladder cancer patients from frozen archived samples. DNA obtained from microdissected tumor and urine sediment was analyzed and compared to peripheral lymphocytes for microsatellite alterations (loss of heterozygosity [L...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19971219)74:6<625::aid-ijc
更新日期：1997-12-19 00:00:00
abstract：:Indoor air pollution (IAP) caused by cooking has been associated with lung cancer risk in retrospective case-control studies in developing and rural countries. We report the association of cooking conditions, fuel use, oil use, and risk of lung cancer in a developed urban population in a prospective cohort of women in...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.29020
更新日期：2015-02-01 00:00:00
abstract：:Neoplastic transformation is characterised by an imbalance in favour of cell growth over programmed cell death (apoptosis). The tumour-suppressor gene p53, responsible for maintaining cell-cycle control, is mutated in the majority of human cancers. Loss of function of the target genes of p53 are therefore important in...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:The current paradigm stipulates that inhibition of histone deacetylase (HDAC) 6 is essential for the combinatorial effect of proteasome and HDAC inhibitors for the treatment of cancers. Our study aims to investigate the effect of combining different class I HDAC inhibitors (without HDAC6 action) with a proteasome inhi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.28924
更新日期：2014-12-15 00:00:00
abstract：:The role of MHC-class-I-antigen expression in intracerebral anti-tumor natural resistance was examined using MHC-positive Lym+ and MHC-negative Lym- lymphoma cell lines. Lym+ was sensitive to MHC-class-I-restricted CTL-mediated lysis, while lym- was resistant. Both lines were susceptible to NK-cell-mediated lysis. The...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(SICI)1097-0215(19960729)67:3<365::AID-IJC
更新日期：1996-07-29 00:00:00
abstract：:Prolonged culture of hepatocytes isolated from mouse liver results in the spontaneous development of colonies of liver epithelial cells that can proliferate indefinitely in vitro. We established 5 such cell lines from C3H/HeJ mice (C3H) and 22 cell lines from C3H/HeJ x C57BL/6J F1 mice (C3B6F1) to investigate whether ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910590120
更新日期：1994-10-01 00:00:00
abstract：:Familial risk of pancreatic cancer has been mainly assessed through case-control studies based on reported but not medically verified cancers in family members. We used the nationwide Swedish Family-Cancer Database on 10.2 million individuals and 21,000 pancreatic cancers to calculate standardized incidence ratios (SI...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.10863
更新日期：2003-02-10 00:00:00
abstract：:Multifocal occurrence and frequent recurrence are characteristic features of urothelial carcinomas of both the urinary bladder and the upper urinary tract. To describe the clonal nature of these tumors, 2 theories have been proposed. The monoclonality hypothesis describes the multiple tumors as descendants of a single...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.10544
更新日期：2002-09-01 00:00:00
abstract：:The targeted radiotherapy of neuroblastoma with 131l-labelled metaiodobenzyl guanidine (mIBG) is now the subject of several clinical studies. The precise intracellular localization of mIBG, necessary for nuclear microdosimetry, has not previously been described. We report the use of electron-energy-loss spectroscopy a...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910470615
更新日期：1991-04-01 00:00:00
abstract：:DNA repair is a complicated biological process, consisting of several distinct pathways, that plays a fundamental role in the maintenance of genomic integrity. The very important field of DNA repair and cancer risk has developed rapidly in the past decades. In this review of selected published data from our laboratory...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.24126
更新日期：2009-03-01 00:00:00
abstract：:Oxaliplatin, which is widely used as chemotherapy for certain solid cancers, frequently causes peripheral neuropathy. Commonly described neuropathic symptoms include aberrant sensations such as mechanical allodynia (hypersensitivity to normally innocuous stimuli). Although oxaliplatin neuropathy is a dose-limiting tox...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32043
更新日期：2019-10-15 00:00:00
abstract：:The aim of this study was to test the utility of AIMP3, an upstream regulator of DNA damage response following genotoxic stress, as a clinical biomarker in muscle-invasive bladder cancer (MIBC). AIMP3 was identified from a meta-analysis of a global gene-expression dataset. AIMP3 protein expression was determined by im...
journal_title：International journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1002/ijc.29022
更新日期：2015-02-01 00:00:00
abstract：:Ionizing radiation remains a major therapeutic tool against human cancers, especially epithelial tumors, which account for the majority of human malignancies. Although Fas and Fas-L are essential determinants of apoptosis, few data support their role in the cytotoxic effect of ionizing radiation. Epstein-Barr-virus (E...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(20000415)86:2<229::aid-ijc
更新日期：2000-04-15 00:00:00
abstract：:The purpose of this study was to induce in postnatal life the selective growth of altered hepatocytic populations which had been induced in utero by various chemicals. Pregnant rats of a Wistar strain were given a single dose of various chemical carcinogens on the 18th gestational day. From two months postpartum, both...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910290317
更新日期：1982-03-15 00:00:00
abstract：:Creatinine-adjusted levels of estrone, estradiol and estriol were determined in overnight urine specimens from 220 post-menopausal women, aged 54 to 66 years, from Boston (121 women) and Athens (99 women). The associations of individual and total estrogens with various characteristics of the women (age at menopause, y...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910400602
更新日期：1987-12-15 00:00:00
abstract：:Human papillomavirus (HPV) is an important factor for the development of tonsillar squamous cell carcinoma (TSCC). In addition, patients with HPV-positive TSCC have a better clinical outcome than patients with HPV-negative TSCC. Although, HPV is an important prognostic marker, additional biomarkers are needed to bette...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.27635
更新日期：2013-01-01 00:00:00
abstract：:Ewing sarcoma (EWS) is the second most common and aggressive type of metastatic bone tumor in adolescents and young adults. There is unmet medical need to develop and test novel pharmacological targets and novel therapies to treat EWS. Here, we found that EWS expresses high levels of a p53 isoform, delta133p53. We fur...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32743
更新日期：2020-06-01 00:00:00
