Immunodetection of cathepsins B and L present in and secreted from human pre-malignant and malignant colorectal tumour cell lines.


:Pre-malignant and malignant human colorectal tumour epithelial cell lines both secreted precursor forms of the 2 cysteine proteinases, cathepsins B and L. The amount of proteinases secreted by these cell lines varied according to the cell density. Comparison at similar cell densities showed that the pre-malignant, adenoma-derived cell line (PC/AA) secreted as much, or more, of both cathepsin B and L precursors as did the malignant, carcinoma-derived cell line (PC/JW/FI). However, mature forms of cathepsins B and L were detected in the culture media of only the carcinoma-derived cell line, thus indicating that the invasive potential of a tumour may be related to its ability to process extracellularly the secreted precursor enzyme to a mature and consequently active enzyme, rather than to the amount of proteinase synthesized and/or secreted. Similar results were obtained using 2 other epithelium-derived tumour cell lines, HT/29 (carcinoma) and SP/AN (adenoma). Immunolocation studies showed that cathepsin B was lysosomal while cathepsin L appeared to have a distribution more consistent with a plasma membrane association. Purified human cathepsins B and L (mature form) were capable of solubilizing an isolated basement membrane matrix (bovine anterior lens capsule) in vitro, thus indicating that the secreted mature enzymes and the membrane-associated cathepsin L could potentially degrade basal laminae or sub-endothelial basement membranes in vivo.


Int J Cancer


Maciewicz RA,Wardale RJ,Etherington DJ,Paraskeva C




Has Abstract


1989-03-15 00:00:00












  • Expression of Von Willebrand factor, an endothelial cell marker, is up-regulated by angiogenesis factors: a potential method for objective assessment of tumor angiogenesis.

    abstract::von Willebrand factor (vWF), a glycoprotein produced uniquely by endothelial cells and megakaryocytes, is routinely used to identify vessels in tissue sections. Vessel density in tumor specimens, as determined by immuno-histochemical staining for vWF or other endothelial cell markers, is a negative prognostic factor f...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zanetta L,Marcus SG,Vasile J,Dobryansky M,Cohen H,Eng K,Shamamian P,Mignatti P

    更新日期:2000-01-15 00:00:00

  • HPV-associated flat penile lesions in men of a non-STD hospital population: less frequent and smaller in size than in male sexual partners of women with CIN.

    abstract::Human papillomavirus (HPV) infections and HPV-associated penile lesions are frequently found in male sexual partners of women with cervical intraepithelial neoplasia (CIN). To determine the significance of these findings, we studied the prevalence of HPV and HPV associated penile lesions in a male hospital population ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bleeker MC,Hogewoning CJ,Voorhorst FJ,van den Brule AJ,Berkhof J,Hesselink AT,Lettink M,Starink TM,Stoof TJ,Snijders PJ,Meijer CJ

    更新日期:2005-01-01 00:00:00

  • The sialosyl Lewis a ganglioside is present in tumorigenic human urothelial cell lines.

    abstract::The sialosyl Lewis a antigen was identified in a ganglioside extract from a malignant human urothelial cell line (Hu 1703He) by fast atom bombardment mass spectrometry. The presence of this antigen in urothelial cell lines with varying tumorigenic properties was further studied using the 19-9 monoclonal antibody (MAb)...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ugorski M,Påhlsson P,Dus D,Nilsson B,Skouv J,Radzikowski C

    更新日期:1990-06-15 00:00:00

  • Reciprocal activating interaction between 6-sulfo LacNAc+ dendritic cells and NK cells.

    abstract::Dendritic cells (DCs) display an extraordinary capacity to induce T-cell responses providing the opportunity of DC-based cancer vaccination strategies. Additional findings indicate that DCs may also play a crucial role for the activation of natural killer (NK) cells, which are important effectors in innate antitumor i...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wehner R,Löbel B,Bornhäuser M,Schäkel K,Cartellieri M,Bachmann M,Rieber EP,Schmitz M

    更新日期:2009-01-15 00:00:00

  • Synchronous multiple primary gastrointestinal cancer exhibits frequent microsatellite instability.

    abstract::Colorectal (CRC) and gastric cancers (GC), the most common gastrointestinal malignancies, have been known to develop occasionally in a same patient. Previous studies have focused on the etiology of patients with multiple primary gastric and colorectal cancer (MPGCC); however, the carcinogenic process of MPGCC remains ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ohtani H,Yashiro M,Onoda N,Nishioka N,Kato Y,Yamamoto S,Fukushima S,Hirakawa-Ys Chung K

    更新日期:2000-06-01 00:00:00

  • Toward a real liquid biopsy in metastatic breast and prostate cancer: Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap).

    abstract::Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL of blood is too small to reliably determine tumor heterogeneity and to be representative as a "liquid biopsy". In the EU FP7 program CTCTrap, we aimed to validate and optimize the recently introduced Diagnostic LeukApheresis (DLA) to screen lit...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Andree KC,Mentink A,Zeune LL,Terstappen LWMM,Stoecklein NH,Neves RP,Driemel C,Lampignano R,Yang L,Neubauer H,Fehm T,Fischer JC,Rossi E,Manicone M,Basso U,Marson P,Zamarchi R,Loriot Y,Lapierre V,Faugeroux V,Oulhen

    更新日期:2018-11-15 00:00:00

  • Human melanoma cells can be killed in vitro by an immunotoxin specific for melanoma-associated antigen p97.

    abstract::Conjugates (immunotoxins) comprising ricin A-chain and monoclonal antibody 96.5, which is specific for human melanoma-associated antigen p97, inhibited protein synthesis and colony formation of cultured human melanoma cells that expressed more than 80,000 molecules of p97 per cell. Cells expressing fewer than 5,000 mo...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Casellas P,Brown JP,Gros O,Gros P,Hellström I,Jansen FK,Poncelet P,Roncucci R,Vidal H,Hellström KE

    更新日期:1982-10-15 00:00:00

  • Male circumcision is associated with a lower prevalence of human papillomavirus-associated penile lesions among Kenyan men.

    abstract::Human papillomavirus (HPV)-associated penile lesions in men may increase the risk of HPV transmission to their female partners. Risk factor data on HPV-associated penile lesions are needed from regions with a high burden of cervical cancer. Visual inspection of the penis was conducted using a colposcope at the 24-mont...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Backes DM,Bleeker MC,Meijer CJ,Hudgens MG,Agot K,Bailey RC,Ndinya-Achola JO,Hayombe J,Hogewoning CJ,Moses S,Snijders PJ,Smith JS

    更新日期:2012-04-15 00:00:00

  • Nasopharyngeal carcinoma-susceptibility locus is localized to a 132 kb segment containing HLA-A using high-resolution microsatellite mapping.

    abstract::Nasopharyngeal carcinoma (NPC) is an epithelial tumor uniquely prevalent in southern Chinese. HLA-A2 is associated with NPC. In a previous study, we showed that the genes associated with susceptibility to NPC are primarily located within the HLA-A locus in Taiwanese NPC patients. However, the pathogenic genes causing ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lu CC,Chen JC,Tsai ST,Jin YT,Tsai JC,Chan SH,Su IJ

    更新日期:2005-07-10 00:00:00

  • CARD9 prevents lung cancer development by suppressing the expansion of myeloid-derived suppressor cells and IDO production.

    abstract::Caspase recruitment domain-containing protein 9 (CARD9) is an adaptor protein and highly expressed in myeloid cells. Our previous study demonstrates a critical protective effect of CARD9 in the development of colitis-associated colon cancer. Nevertheless, the effect of CARD9 in lung cancer remains unclear. Here, using...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Qu J,Liu L,Xu Q,Ren J,Xu Z,Dou H,Shen S,Hou Y,Mou Y,Wang T

    更新日期:2019-10-15 00:00:00

  • Oncostatic activity of a thiazolidinedione derivative on human androgen-dependent prostate cancer cells.

    abstract::Thiazolidinedione derivatives with potent antiarthritic activity, such as CGP 52608, have been suggested to exert their biological effects through the activation of the orphan nuclear receptor RORalpha. Since response elements for this receptor are present in the promoter region of cell cycle-related genes (i.e., p21(...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Moretti RM,Montagnani Marelli M,Motta M,Limonta P

    更新日期:2001-06-01 00:00:00

  • Lysis of RSV-transformed Japanese quail cells by a factor from normal quail serum.

    abstract::Normal sera of Japanese quails caused lysis of tumor cells from a Rous sarcoma virus (RSV)-induced quail tumor (QT cells). Other tumor cell lines, including RSV-transformed quail embryo cells and methylcholanthrene-induced quail tumors, were not lysed. This naturally occurring cytolytic factor (NCLF) was sensitive to ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yoshikawa Y,Yamanouchi K,Hishiyama M,Kobune K

    更新日期:1978-05-15 00:00:00

  • Clinicopathologic significance of urokinase receptor- and MMP-9-positive stromal cells in human colorectal cancer: functional multiplicity of matrix degradation on hematogenous metastasis.

    abstract::Our previous clinicopathologic study revealed an inverse association of liver metastasis of colorectal cancer and stromal expression of matrix metalloproteinase-9 (MMP-9) or urokinase receptor (uPAR). This suggests that host cells, particularly macrophages, expressing matrix-degrading enzymes/factors could be protecti...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Saito K,Takeha S,Shiba K,Matsuno S,Sorsa T,Nagura H,Ohtani H

    更新日期:2000-04-01 00:00:00

  • The presence of concanavalin-A(Con-A)-like molecules on natural-killer (NK)-sensitive target cells: their possible role in swainsonine-augmented human NK cytotoxicity.

    abstract::In the present study we examined the expression of concanavalin-A(Con-A)-like molecules on natural-killer (NK)-sensitive target cells and investigated their possible role in the human NK-cell phenomenon. The incubation of either peripheral-blood lymphocytes (PBL) or large granular lymphocytes (LGL) with swainsonine (S...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yagita M,Noda I,Maehara M,Fujieda S,Inoue Y,Hoshino T,Saksela E

    更新日期:1992-10-21 00:00:00

  • The evolving epidemic of breast cancer in sub-Saharan Africa: Results from the African Cancer Registry Network.

    abstract::Breast cancer (BC) is the leading cause of cancer in sub-Saharan Africa (SSA) with rapidly increasing incidence rates reported in Uganda and Zimbabwe. However, the magnitude of these rising trends in premenopausal and postmenopausal women is unknown in most African countries. We used data from the African Cancer Regis...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Joko-Fru WY,Jedy-Agba E,Korir A,Ogunbiyi O,Dzamalala CP,Chokunonga E,Wabinga H,Manraj S,Finesse A,Somdyala N,Liu B,McGale P,Jemal A,Bray F,Parkin DM

    更新日期:2020-10-15 00:00:00

  • Human astrocytomas and glioblastomas express monocyte chemoattractant protein-1 (MCP-1) in vivo and in vitro.

    abstract::Expression of the monocyte chemoattractant protein-1 (MCP-1) was examined in human central nervous system tumours (glioblastomas and astrocytomas) and normal human brain. Northern blot analysis demonstrated constitutive expression of MCP-1 mRNA in 6 of 12 glioblastoma cell lines. Expression could be stimulated by inte...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Desbaillets I,Tada M,de Tribolet N,Diserens AC,Hamou MF,Van Meir EG

    更新日期:1994-07-15 00:00:00

  • Metastatic tumor cells adhere preferentially to the extracellular matrix underlying vascular endothelial cells.

    abstract::Two metastatic cell lines, mouse B16-Fl melanoma and human Hs939 melanoma, were examined for their abilities to adhere to confluent vascular endothelial cell monolayers and to the underlying endothelial extracellular matrix. Tumor cells attacked slowly to the endothelial cell monolayers while they adhered rapidly to i...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kramer RH,Gonzalez R,Nicolson GL

    更新日期:1980-11-15 00:00:00

  • Lymphocyte stimulation by autologous tumor cells in the presence of serum from the same patient or from healthy donors.

    abstract::Lymphocyte blastogenesis induced by autologous tumor biopsy cells, by allogeneic lymphocytes and by PHA, was performed in the presence of autologous or allogeneic serum collected from normal blood donors. In 13 of the 15 cases lymphocyte stimulation by autologous tumor cells was inhibited in the presence of autologous...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Vánky F,Klein E,Stjernswärd J,Trempe G

    更新日期:1975-11-15 00:00:00

  • HER2/neu over-expression induces endothelial cell retraction.

    abstract::Over-expression of the HER2/neu (HER2) proto-oncogene in breast carcinoma imparts an enhanced metastatic potential. Metastasis requires escape of the tumor cell from the vasculature into subjacent tissue, a transmigration event across an endothelial cell (EC) monolayer. EC retraction has been reported to precede trans...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Carter WB,Hoying JB,Boswell C,Williams SK

    更新日期:2001-02-01 00:00:00

  • The association between change in body mass index and upper aerodigestive tract cancers in the ARCAGE project: multicenter case-control study.

    abstract::Previous studies reported an inverse relationship between body mass index (BMI) and upper aerodigestive tract (UADT) cancers. Examining change in BMI over time may clarify these previous observations. We used data from 2,048 cases and 2,173 hospital- and population-based controls from ten European countries (alcohol-r...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Park SL,Lee YC,Marron M,Agudo A,Ahrens W,Barzan L,Bencko V,Benhamou S,Bouchardy C,Canova C,Castellsague X,Conway DI,Healy CM,Holcátová I,Kjaerheim K,Lagiou P,Lowry RJ,Macfarlane TV,Macfarlane GJ,McCartan BE,McKinn

    更新日期:2011-03-15 00:00:00

  • Clinical and biological significance of HSP89 alpha in human breast cancer.

    abstract::In order to isolate and characterize genes whose expression may be altered in breast malignancy, we screened a cDNA library with a polyclonal anti-serum against breast-cancer-metastasis membranes and isolated several immunopositive clones. One of these, AJ1, was analyzed in detail and found to be expressed at varying ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Jameel A,Skilton RA,Campbell TA,Chander SK,Coombes RC,Luqmani YA

    更新日期:1992-02-01 00:00:00

  • Correlation between cytogenetic data and ganglioside pattern in human meningiomas.

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    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Berra B,Papi L,Bigozzi U,Serino D,Morichi R,Mennonna P,Rapelli S,Cogliati T,Montali E

    更新日期:1991-02-01 00:00:00

  • A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis.

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    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hasegawa K,Ono T,Matsushita H,Shimono M,Noguchi Y,Mizutani Y,Kodama J,Kudo T,Nakayama E

    更新日期:2004-01-01 00:00:00

  • Epithelial-to-mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities.

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    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Ling Z,Cheng B,Tao X

    更新日期:2020-10-22 00:00:00

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    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Weiss H,Amberger A,Widschwendter M,Margreiter R,Ofner D,Dietl P

    更新日期:2001-06-15 00:00:00

  • Treatment of melanoma of unknown primary in the era of immunotherapy and targeted therapy: A Dutch population-based study.

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    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Verver D,van der Veldt A,van Akkooi A,Verhoef C,Grünhagen DJ,Louwman WJ

    更新日期:2020-01-01 00:00:00

  • Advanced paternal age and childhood cancer in offspring: A nationwide register-based cohort study.

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    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Urhoj SK,Raaschou-Nielsen O,Hansen AV,Mortensen LH,Andersen PK,Nybo Andersen AM

    更新日期:2017-06-01 00:00:00

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    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Furumoto K,Arii S,Yamasaki S,Mizumoto M,Mori A,Inoue N,Isobe N,Imamura M

    更新日期:2000-09-01 00:00:00

  • Recognition by a natural cytotoxic antibody of lactoneotetraglycosyl ceramide as an antigenic molecule in a syngeneic rat fibrosarcoma.

    abstract::Conventionally bred rats possess in their sera an NA which has a cytotoxic effect on a tumor cell line (KMT-17) derived from a fibrosarcoma induced by 3-methylcholanthrene and which can be absorbed by normal rat tissues. We have succeeded in purifying a glycosphingolipid as an antigen reactive to NA. GSLs isolated fro...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Takimoto M,Suzuki E,Naiki M,Itaya T,Takeichi N,Hosokawa M,Kobayashi H

    更新日期:1985-12-15 00:00:00

  • Losses of the tumor suppressor BIN1 in breast carcinoma are frequent and reflect deficits in programmed cell death capacity.

    abstract::Oncogenic activation of MYC occurs often in breast carcinoma and is associated with poor prognosis. Loss or inactivation of mechanisms that restrain MYC may therefore be involved in tumor progression. In this study, we show that the MYC-interacting adaptor protein BIN1 is frequently missing in malignant breast cells a...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ge K,Duhadaway J,Sakamuro D,Wechsler-Reya R,Reynolds C,Prendergast GC

    更新日期:2000-02-01 00:00:00