Abstract:
:The large geographic variations in the incidence of gastric cancer (GC) are likely due to differential environmental exposures, in particular to Helicobacter pylori (H. pylori) infection. We aimed to investigate the impact of H. pylori on the epigenome in normal gastric mucosa and methylation changes associated with cancer risk independent of H. pylori. A discovery set of normal gastric mucosa from GC cases (n = 42) and controls (n = 42), nested in a large case-control study and stratified by H. pylori status, were subjected to genome-wide methylation profiling. Single-nucleotide polymorphism arrays from peripheral blood leukocytes were used to conduct methylation quantitative trait loci (mQTL) analysis. A validation set of gastric mucosa samples (n = 180) was used in the replication phase. We found 1,924 differentially methylated positions (DMPs) and 438 differentially methylated regions (DMRs) associated with H. pylori infection, most of which were hypermethylated. Significant methylation alterations identified in the initial set were successfully replicated. Furthermore, the H. pylori-associated DMP/Rs showed marked stability ('epigenetic memory') after H. pylori clearance. Interestingly, we found 152 DMRs associated with cancer risk independent of the H. pylori status in normal gastric mucosa. The methylation score derived from three biomarkers was a strong predictor of GC. Finally, the mQTL analysis indicated that the H. pylori- and cancer-specific methylation signatures were minimally affected by genetic variation. The comprehensively characterized methylome changes associated with H. pylori infection and GC risk in our study might serve as potential biomarkers for early cancer progression in tumour-free gastric mucosa.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Woo HD,Fernandez-Jimenez N,Ghantous A,Degli Esposti D,Cuenin C,Cahais V,Choi IJ,Kim YI,Kim J,Herceg Zdoi
10.1002/ijc.31381subject
Has Abstractpub_date
2018-08-01 00:00:00pages
597-609issue
3eissn
0020-7136issn
1097-0215journal_volume
143pub_type
杂志文章abstract::Use of dietary supplements is rising in countries where colorectal cancer is prevalent. We conducted a systematic literature review and meta-analyses of prospective cohort studies on dietary supplement use and colorectal cancer risk. We identified relevant studies in Medline, Embase and Cochrane up to January 2013. Or...
journal_title:International journal of cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1002/ijc.29277
更新日期:2015-05-15 00:00:00
abstract::In this prospective study, the relationship between energy and fat consumption and the risk of breast cancer was examined. Between 1977 and 1983, 31,209 Norwegian women, 20 to 54 years of age attended a health screening. The attendees were given a food-frequency questionnaire to be completed at home, and this was retu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910630104
更新日期:1995-09-27 00:00:00
abstract::The prevalence and clinical relevance of TP53 germline mutations in a large unselected breast cancer series are largely unknown. Here, we determined TP53 germline mutations in a large cohort of 10,053 unselected breast cancer patients through multigene panel-based next-generation and/or Sanger sequencing assays. We fo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32424
更新日期:2020-01-15 00:00:00
abstract::Cetuximab is a human/mouse chimeric IgG1 monoclonal antibody (mAb) to epidermal growth factor receptor, approved for colorectal carcinoma treatment in combination with chemotherapy. The immune-mediated effects elicited by its human fraction of crystallization moiety might critically contribute to the overall anti-tumo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26181
更新日期:2012-04-01 00:00:00
abstract::A previously published case-control study of agricultural risk factors involved male cases of non-Hodgkin's lymphoma registered under code 202 of the International Classification of Diseases (ICD). This study has been expanded with the inclusion of cases registered under ICD code 200, and additional controls. The expa...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390206
更新日期:1987-02-15 00:00:00
abstract::The neural crest gives rise to a variety of tissues, including peripheral neurons, Schwann cells, melanocytes and ectomesenchymal cells, which include the smooth-muscle cells of large arteries. Cell lines derived from neuroblastoma (a neural-crest tumor) exhibit at least 2 distinct morphological cell types, a neurobla...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480221
更新日期:1991-05-10 00:00:00
abstract::The melanosomal protein TRP2 expressed by melanocytes and most melanoma cells is an attractive, clinically relevant model antigen for the experimental development of melanoma immunotherapy in mice. A peptide shared by murine and human TRP2 can be recognized by melanoma-reactive CTL in C57BL/6 mice, as well as in human...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000401)86:1<89::aid-ijc1
更新日期:2000-04-01 00:00:00
abstract::A human papillomavirus (HPV) was cloned from a patient with multiple squamous cell carcinomas (SCCs) and identified as HPV88, recently categorized into a new species within the genus Gamma. The HPV88 viral load in an SCC of the index patient exceeded 1 million copies/cell. By contrast, a survey of 447 skin lesions (79...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23115
更新日期:2008-01-15 00:00:00
abstract::Lymphoma EL-4 of B6 origin was inoculated into lethally-irradiated syngeneic B6 or resistant BD2F1 hybrid recipients. Marked impairment of lymphoma cell proliferation (i.e. hybrid resistance, HR) occurred in the spleen but not in the liver or lung of BD2F1 hosts. Treatment with DTIC at the optimal dose of 80 mg/kg i.p...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280416
更新日期:1981-10-15 00:00:00
abstract::Azathioprine (AZ) was injected into C57 Black mice before or after IM implantation of the syngeneic fibrosarcoma FS6, and observations were made on tumour growth and on the cellular composition of the fibrosarcomas. When multiple injections were given to the tumour-bearing host the tumours regressed. The percentage of...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910200119
更新日期:1977-07-15 00:00:00
abstract::Several lines of evidence suggest that long-term treatment with non-steroidal anti-inflammatory drugs may reduce the risk of colon cancer and the size and number of colonic polyps in patients with familial adenomatous polyposis. Aspirin has also been shown to inhibit cell proliferation in human tumor cell lines and to...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19971210)73:6<880::aid-ijc
更新日期:1997-12-10 00:00:00
abstract::Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL of blood is too small to reliably determine tumor heterogeneity and to be representative as a "liquid biopsy". In the EU FP7 program CTCTrap, we aimed to validate and optimize the recently introduced Diagnostic LeukApheresis (DLA) to screen lit...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.31752
更新日期:2018-11-15 00:00:00
abstract::Changes in the expression of a cell membrane antigen during hepatocarcinogenesis and in the developing liver were analyzed by HAM.4, a monoclonal antibody (MAb) against a membrane glycoprotein of normal rat hepatocyte. Of the precancerous lesions observed during hepatocarcinogenesis induced by diethylnitrosamine, 2-ac...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910410418
更新日期:1988-04-15 00:00:00
abstract::The tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) was found to increase the incidence of phenotypic alterations induced by the direct-acting carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in human endometrial stromal cells. Following carcinogen treatment, changes in saturation density, gamma-glutam...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910320405
更新日期:1983-10-15 00:00:00
abstract::Twenty-seven human colorectal adenocarcinomas were implanted s.c. into nude mice. A comparative study of chromosomes and DNA between fresh tumor (FT) and xenografted tumor cells (XT) could be performed in 9 cases. Losses or deletions were consistently found in FTs as well as in XTs, particularly on chromosomes 17 and/...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440521
更新日期:1989-11-15 00:00:00
abstract::In ovarian cancer cells, the macrophage colony-stimulating factor-1 (CSF-1) induces the release of plasminogen activator inhibitor-2 (PAI-2), and high levels of PAI-2 as well as of CSF-1 in ovarian cancer ascites are independently correlated with poor outcome. We now study the effect of CSF-1 on PAI-2 expression in vi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19971219)74:6<571::aid-ijc
更新日期:1997-12-19 00:00:00
abstract::Smoking is indisputably linked to lung cancer, yet only a small fraction of smokers develops this disease. Although previously tobacco-derived carcinogens and enzyme polymorphisms have been identified to increase the risk for smokers, recent epidemiological data suggest even sex-specificity as a new and additional fac...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.32716
更新日期:2020-05-01 00:00:00
abstract::Interval breast cancer (IC) has a more aggressive phenotype and higher mortality than screen-detected cancer (SDC). In this case-case study, we investigated whether the size of longitudinal fluctuations in mammographic percent density (PD fluctuation) was associated with the ratio of IC versus SDC among screened women...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30427
更新日期:2017-01-01 00:00:00
abstract::A challenging task of the immune system is to fight cancer cells. However, a variety of human cancers educate immune cells to become tumor supportive. This is exemplified for tumor-associated macrophages (TAMs), which are polarized towards an anti-inflammatory and cancer promoting phenotype. Mechanistic explanations, ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24594
更新日期:2009-11-01 00:00:00
abstract::By rosetting with SRBC coupled to rabbit-anti-human IgM, the surface IgM-negative cells of human fetal bone marrow were enriched, and subsequently infected and transformed by Epstein-Barr virus (EBV). Single clones of the transformed cells were obtained. Ninety percent of the resulting cell clones were surface-immunog...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390212
更新日期:1987-02-15 00:00:00
abstract::Vitamin D is well known for its important role in calcium and phosphor homeostasis. Recent research suggests that vitamin D also prevent some type of cancers. We studied solar vitamin D effective UV radiation (VD dose), dietary vitamin D, sun-seeking holidays, use of solarium, frequency of sunburn and breast cancer ri...
journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.25463
更新日期:2011-03-15 00:00:00
abstract::The presence of TGF alpha mRNA has been reported previously to occur in primary colon cancers. We report the expression of the normal 4.5 kb TGF alpha transcript in the mucosa of the normal human gastro-intestinal tract from oesophagus through to colon. The highest levels of human TGF alpha mRNA occurred in the duoden...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430305
更新日期:1989-03-15 00:00:00
abstract::Many studies have focused on global hypomethylation or hypermethylation of tumor suppressor genes, but less is known about the impact of promoter hypomethylation of oncogenes. We previously showed that promoter methylation may gradually increase or decrease during the transition from gastric mucosa (GM) to intestinal ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32946
更新日期:2020-06-15 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is a cell plasticity process required for metastasis and chemoresistance of carcinoma cells. We report a crucial role of the signal adaptor proteins CRK and CRKL in promoting EMT and tumor aggressiveness, as well as resistance against chemotherapy in colorectal and pancreatic ca...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32955
更新日期:2020-09-15 00:00:00
abstract::In order to analyze the mutational events and to understand the biological significance of the p53 gene in chemical carcinogenesis, we applied a new yeast-based p53 functional assay to ovarian tumors induced by 7, 12-dimethylbenz[a]anthracene (DMBA), as well as to transitional cell carcinomas of the urinary bladder in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19991126)83:5<700::aid-ijc
更新日期:1999-11-26 00:00:00
abstract::Interaction between CD40 and its ligand, CD154, has a key function in immune regulation. Recent experimental data support a role of deregulated CD40 signalling in lymphomagenesis. Data from earlier studies that are part of this pooling study implicate a functional polymorphism (-1C>T, rs1883832) in the TNFRSF5 gene en...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/ijc.25420
更新日期:2011-03-15 00:00:00
abstract::The relationship between the strength of the estrogenic and progestational components of combined oral contraceptives and risk of endometrial carcinoma was examined in a multinational hospital-based case-control study comparing 220 cases from 7 countries with 1,537 age- and hospital-matched controls. Oral contraceptiv...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490612
更新日期:1991-12-02 00:00:00
abstract::The mannose-6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R) encodes a protein that plays a critical role in tumor suppression, in part by modulating bioavailability of a potent mitogen, insulin-like growth factor-2 (IGF2). We tested the hypothesis that the common nonsynonymous genetic variants in M6P/IGF...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.24623
更新日期:2009-12-01 00:00:00
abstract::Dietary intake of many fruits and vegetables has been shown to be associated with reduced risk of cancer. We investigated the in vivo efficacy of grape seed extract (GSE, patented as Traconol) against prostate cancer (PCA) and associated molecular events. Athymic nude mice were implanted with hormone-refractory human ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11620
更新日期:2004-02-20 00:00:00
abstract::The breakpoint of the 18q21 translocation of B-cell-non-Hodgkin's lymphoma (NHL) cell line Karpas1106P was delineated by fluorescence in situ hybridization (FISH). Karpas1106P was derived from mediastinal lymphoblastic B-cell lymphoma and exhibited the immunophenotype characteristic of marginal-zone B-cell lymphoma (M...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980925)78:1<100::aid-ijc
更新日期:1998-09-25 00:00:00