The use of the D-, L- aspartic ratio in decalcified collagen from human dentin as an estimator of human age.

Abstract:

:Among the methods dealing with the age estimation, the evaluation of the ratio of the D-, L- form of the aspartic acid in tissues with a low metabolic turnover is considered to be the most precise. We introduced demineralization of the dentin with 0.5 M EDTA adjusted to pH = 7.4. The advantage of such a procedure is that after demineralization we obtained pure insoluble protein (collagen) and soluble noncollagenous proteins in one step. In this study we analyzed insoluble collagen. The amino acids obtained after the hydrolysis were derivatized into TFA isopropyl esters and analyzed by gas chromatography on Chirasil L-Val capillary column. We analyzed human dentin from the lower canines. The correlation coefficient was 0.93 for our set of 71 persons. The result concurred with those of other scientists.

journal_name

J Forensic Sci

authors

Pilin A,Cabala R,Pudil F,Bencko V

keywords:

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

1228-31

issue

5

eissn

0022-1198

issn

1556-4029

journal_volume

46

pub_type

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