Abstract：
:Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The products of twelve animal DNAs (human, Japanese monkey, dog, cattle, pig, cat, rabbit, mouse, rat, chicken, frog, and fish) were separated by polyacrylamide gel electrophoresis, each revealing a few bands ranging from 150 to 100 base pairs. The band patterns obtained from each DNA sample differed in number and size, which indicates the applicability of the method to species identification. Samples containing either as little as 1 pg of DNA or degraded DNA of 0.2 to 0.5 kb in length were able to give detectable bands. Postmortem human tissue DNAs were tested as an example. They showed a pattern identical to the human control one, which was distinct from those of the other animals examined.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Naito E,Dewa K,Ymanouchi H,Kominami Rkeywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
396-403issue
2eissn
0022-1198issn
1556-4029journal_volume
37pub_type
杂志文章abstract：:This retrospective study examined population demographics associated with motor vehicle collision (MVC) fatalities over a 5-year period in the Hamilton-Wentworth Niagara region. Variables were drawn from the five factors proposed by Fierro (1) for investigating deaths caused by transportation: human, chemical, environ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00723.x
更新日期：2008-05-01 00:00:00
abstract：:Solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) was used to characterize the volatile components associated with U.S. currency, U.S. currency inks, and Canadian currency. Compounds that can be attributed to the ink-curing process include series of straight-chain aldehydes, alkenals, acids...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:Suicides due to fall from height in Geneva from 1991 to 2000 were reviewed. Scene investigations, autopsy findings, psychiatric histories, and toxicology results were examined. There were 197 of these suicides, an incidence of five cases per 100,000 inhabitants per year or one fourth of all suicides in Geneva per year...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:Using GC/MS in scanning mode as a screening and definitive identification methodology for substance abuse testing, 4500 urine samples have been analyzed. The accuracy and sensitivity of this method was evaluated by the results of 92 proficiency sample analyses, reanalyses by TLC screening with GC confirmation of 125 s...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Reported herein is the suicidal asphyxiation of a young man due to exhaustion of oxygen in the interior of a sealed automobile into which the exhaust emissions were diverted. His blood carboxyhemaglobin concentration was less than 5% saturation. The car was equipped with a catalytic converter and when tested, the exha...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00224.x
更新日期：2006-09-01 00:00:00
abstract：:Purification methods of deoxyribonucleic acid (DNA) from degraded and contaminated forensic samples were investigated for polymerase chain reaction (PCR) analysis. DNA extracted from putrefied tissue or bloodstains sometimes contained the copurified contaminant, that was identified as the porphyrin compound (hematin)....
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
abstract：:Ethanol concentrations were measured in femoral venous blood in deaths attributed to acute alcohol poisoning (N = 693) or chronic alcoholism (N = 825), according to the forensic pathology report. Among acute alcohol poisonings were 529 men (76%) with mean age 53 years and 164 women (24%) with mean age 53 years. In the...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:An improved technique for recording infrared spectra from minute amounts of synthetic fibers by using KBr micropellets is described. The procedure is the same for all commonly encountered synthetic fiber varieties; hence, no preliminary identification is required. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：:The AmpFℓSTR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y-STR loci is a more efficient analytical mode for poo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12950
更新日期：2016-03-01 00:00:00
abstract：:This paper constitutes a feasibility report on the use of the human leukocyte antigen (HLA) system for the typing of dried bloodstains. Antigens tested include the HLA-A2, A3, A10, B7, B8, and B14 alleles. An aging study conducted on A3 positive bloodstains showed that HLA-A3 could be reliably detected on bloodstains ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:This study uses the naturalistic decision-making (NDM) perspective to examine how Dutch forensic team leaders (i.e., the officers in charge of criminal forensic research from the crime scene until the use of laboratory assistance) make decisions in real-life settings and identifies the contextual factors that might in...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01714.x
更新日期：2011-07-01 00:00:00
abstract：:Fatal traumatic thrombosis of the left internal carotid artery occurred in a 38-year-old man following minor blunt cervical trauma during an altercation. There was no external injury observed on the head, face, or neck. Neurologic deficit developed soon after the injury, which progressed to right hemiplegia. His condi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:In bite mark analyses, the initial photograph is critical for the collection and presentation of evidence. A high-contrast film technique previously used primarily in the graphic arts field has been refined and applied to forensic odontology. The process, called toneline, reduces the interpretational bias of the inves...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Based on the phenomenon that static electricity attracts dust, a polyethylene terephthalate (PET) semirigid sheet coated with printing ink was used to visualize impressions of fingerprints on the skin of living and dead bodies. Compared with visualization methods for perspiration fingerprints, this method recovers bet...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Positive identification of human remains is often achieved by comparing antemortem and postmortem radiographs. Usually, radiographs contain a number of markers that can serve as reference for comparison, one of these markers is the trabecular bone pattern depicted in the roentgenograms. In the present case, densitomet...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:In general, hanging cases are the result of suicide, and accidental and homicidal hanging cases are rarely seen. This study retrospectively investigated 4571 death examinations and autopsies that were performed at The Konya Branch of the Forensic Medicine Council (Turkey) between 1998 and 2007; hanging was involved in...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01320.x
更新日期：2010-05-01 00:00:00
abstract：:In the era of Daubert and other judicial rulings pertaining to the acceptability of forensic evidence, it is increasingly important that experts are able to testify that their methods have been scientifically tested and that error rates and other factors relating to reliability have been published. The purpose of this...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:The two cases presented here involve deaths that were due to dichloromethane or chloroform poisoning. The dichloromethane and chloroform were determined in biological specimens by headspace gas chromatography with mass spectrometric detector. In one case, dichloromethane concentrations found were 252 mg/L (blood), 75 ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:Two hundred and twenty-eight consecutive medicolegal autopsies were studied as to the cause of death established by a physician, the mode of death as suggested in the police report, and findings of medical interest often discovered following autopsy. Corrections had to be made even in some cases which seemed to be obv...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:A blind quality control (QC) program was successfully developed and implemented in the Toxicology, Seized Drugs, Firearms, Latent Prints (Processing and Comparison), Forensic Biology, and Multimedia (Digital and Audio/Video) sections at the Houston Forensic Science Center (HFSC). The program was put into practice base...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14259
更新日期：2020-05-01 00:00:00
abstract：:A simple method for quantification of contrast in a fingerprint is proposed. Contrast is defined as the average difference in intensity of pixels between valleys and ridges in a fingerprint. It is quantified from a scanner-acquired image of the fingerprint using a histogram function of Adobe Photoshop. The method was ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12224
更新日期：2013-09-01 00:00:00
abstract：:Visualization of nitrite residues is essential in gunshot distance determination. Current protocols for the detection of nitrites include, among other tests, the Modified Griess Test (MGT). This method is limited as nitrite residues are unstable in the environment and limited to partially burned gunpowder. Previous re...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13802
更新日期：2019-01-01 00:00:00
abstract：:A study was conducted of potential vapor phase interferents which could be present on human breath and also be capable of inducing a false-positive response for ethanol on the evidential infrared-based breath testing device, the Intoxilyzer-5000. This involved preparation and validation of a range of vapor standards, ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Opium is the raw material for the production of heroin, and the characterization of opium seizures through laboratory analysis is a valuable tool for law enforcement agencies to trace clandestine opium production and trafficking. In this work, a method for opium profiling based on the relative content of five principa...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13190
更新日期：2016-11-01 00:00:00
abstract：:In 2012, the Harris County Institute of Forensic Sciences began prospectively collecting injury data from pediatric autopsies. These data and associated case information from 635 pediatric cases are archived in the Infant Injury Database (IID). This paper introduces the IID to the forensic community and demonstrates i...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14120
更新日期：2019-11-01 00:00:00
abstract：:In this study, the nondestructive differentiation of individual white polyester clothing fibers was accomplished via synchrotron radiation microbeam X-ray fluorescence (SR-μ-XRF) analysis. SR-μ-XRF with vertical focusing is a useful nondestructive method for the analysis of a single polyester clothing fiber. Kirkpatri...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14481
更新日期：2020-09-01 00:00:00
abstract：:The third edition of the Diagnostic and Statistical Manual of the American Psychiatric Association (DSM-III) is evaluated from the standpoint of forensic science experience in a family court setting. The importance of diagnosis in developing pertinent recommendations within an adversarial system is discussed, with par...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Latent fingerprint visualization on discharged shell casings can provide good forensic evidence, particularly if the casing is recovered at the scene of a crime where a firearm has been discharged. Unfortunately, visualization of such latent fingerprints when they were deposited prior to discharge of the firearm is pr...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01059.x
更新日期：2009-07-01 00:00:00
abstract：:The U.S. agricultural sector is vulnerable to intentionally introduced microbial threats because of its wide and open distribution and economic importance. To investigate such events, forensically valid assays for plant pathogen detection are needed. In this work, real-time PCR assays were developed for three model pl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12321
更新日期：2014-03-01 00:00:00
abstract：:In Italy, the "silent-consent" principle of donor's willingness regulates organ donation for postmortem transplantation, but civil incompetence excludes it. We investigated decisional capacity for organ donation for transplantation of 30 controls and 30 nonincompetent patients with schizophrenia as related to clinical...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01364.x
更新日期：2010-05-01 00:00:00
