Abstract：
:Gamma-hydroxybutyrate (GHB) is a prescribed medication as well as a drug of abuse. Its detection in various matrices for in-field forensic scientists remains a challenge. We have developed an assay that uses aldo-keto reductase 7A2 (AKR7A2) for the specific determination of GHB in various drinks. AKR7A2 was purified using Ni-affinity chromatography. The Michaelis-Menten constant for the GHB oxidation reaction was 10 mM, and the minimum detection limit was 4 mM. Ethanol was not a substrate for AKR7A2. In a coupled reaction with NADP(+), phenazine methosulfate (PMS), and 2,6-dichlorophenolindophenol, various beverages (orange juice, milk, soda, and numerous alcoholic drinks) containing GHB turned from blue to light yellow. In a second coupled reaction where diaphorase replaced PMS, the presence of GHB also caused the expected change of color in various beers.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Bendinskas K,Sattelberg P,Crossett D,Banyikwa A,Dempsey D,MacKenzie JAdoi
10.1111/j.1556-4029.2010.01694.xsubject
Has Abstractpub_date
2011-05-01 00:00:00pages
783-7issue
3eissn
0022-1198issn
1556-4029journal_volume
56pub_type
杂志文章abstract：:Papaveris pericarpium, a natural source of morphine and codeine, is the principal active component in many antitussive traditional Chinese medicines. We herein report the first PK study of papaveris pericarpium in human plasma and urine following oral administration of single (15, 30, 60 mL) and multiple dose (15 mL) ...
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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doi：
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journal_title：Journal of forensic sciences
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