Abstract:
:The AmpFℓSTR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y-STR loci is a more efficient analytical mode for poorly preserved human femurs (n = 15) discovered at Korean archeological sites. To reveal locus profiles not easily obtained by Yfiler analysis, custom-designed primers were adopted for the DYS390, DYS391, DYS392, DYS438, DYS439, and DYS635 loci. The success rate for 16 Y-STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Yfiler kit only) to 49.17% (the coupled use of Yfiler and custom-designed primers). In this study, the authors established that the custom-designed primers offer a markedly improved success rate for obtainment of Y-STR profiles from degraded aDNA not easily identified by sole use of the Yfiler assay.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Oh CS,Lee SD,Shin KJ,Shin DHdoi
10.1111/1556-4029.12950subject
Has Abstractpub_date
2016-03-01 00:00:00pages
430-438issue
2eissn
0022-1198issn
1556-4029journal_volume
61pub_type
杂志文章abstract::Seven subjects participated in a two-part study to evaluate mouth alcohol dissipation in alcohol positive subjects. In part one, subjects rinsed their mouths with a vodka solution and were breath tested after 1, 2, 3, 4, and 5 min intervals. On average, breath alcohol concentration (BrAC) decreased 20.4% (range 3.2-47...
journal_title:Journal of forensic sciences
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journal_title:Journal of forensic sciences
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doi:10.1111/1556-4029.13752
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.14235
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
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journal_title:Journal of forensic sciences
pub_type: 历史文章,杂志文章
doi:
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.12520
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:2005-09-01 00:00:00
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journal_title:Journal of forensic sciences
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doi:10.1111/1556-4029.13884
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.13044
更新日期:2016-05-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2012.02287.x
更新日期:2013-01-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Anomalous origin of the coronary artery (AOCA) is a rare, but important cause of sudden cardiac death among young athletes. Nine autopsy cases (8 male, 1 female; mean age, 17.9 years; age range, 11-31 years) of sudden death during or just after exercise caused by AOCA were reviewed. The exercises performed at the time...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.13004
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pub_type: 杂志文章
doi:10.1111/j.1556-4029.2010.01604.x
更新日期:2011-01-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2009.01222.x
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.13870
更新日期:2019-03-01 00:00:00
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journal_title:Journal of forensic sciences
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doi:10.1111/1556-4029.12947
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章,评审
doi:
更新日期:2001-09-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.13476
更新日期:2018-01-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type:
doi:10.1111/1556-4029.14041
更新日期:2019-09-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
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journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2006.00228.x
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