Abstract：
:A forensic genotyping panel of 11 tetranucleotide STR loci from the domestic cat was characterized and evaluated for genetic individualization of cat tissues. We first examined 49 candidate STR loci and their frequency assessment in domestic cat populations. The STR loci (3-4 base pair repeat motifs), mapped in the cat genome relative to 579 coding loci and 255 STR loci, are well distributed across the 18 feline autosomes. All loci exhibit Mendelian inheritance in a multi-generation pedigree. Eleven loci that were unlinked and were highly heterozygous in cat breeds were selected for a forensic panel. Heterozygosity values obtained for the independent loci, ranged from 0.60-0.82, while the average cat breed heterozygosity obtained for the 11 locus panel was 0.71 (range of 0.57-0.83). A small sample set of outbred domestic cats displayed a heterozygosity of 0.86 for the 11 locus panel. The power of discrimination of the panel is moderate to high in the cat breeds examined, with an average P(m) of 3.7E-06. The panel shows good potential for genetic individualization within outbred domestic cats with a P(m) of 5.31E-08. A multiplex protocol, designed for the co-amplification of the 11 loci and a gender-identifying locus, is species specific and robust, generating a product profile with as little as 0.125 nanograms of genomic DNA.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Menotti-Raymond MA,David VA,Wachter LL,Butler JM,O'Brien SJkeywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
1061-70issue
5eissn
0022-1198issn
1556-4029journal_volume
50pub_type
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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更新日期：1992-03-01 00:00:00
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
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journal_title：Journal of forensic sciences
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doi：
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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pub_type： 杂志文章,评审
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