Abstract:
:The extraction of DNA from human skeletal remains applied to forensic, and evolutionary studies do not exclude risks, which are to be evaluated when working with unique specimens that could be damaged or even destroyed. In the present study were evaluated several nondestructive methods for recovering DNA instead of the most currently used pulverization method. Three different procedures to access inside the dental pieces (occlusal perforation, cervical perforation, and cervical cut) have been compared with the aim of recovering as many cell remains as possible to carry out a DNA extraction. Given the DNA quantitation results, a method was proposed that consists of a cervical cut to facilitate the access to the pulp cavity and a subsequent filing of the root canals down to the apex of the dental root. This methodology allows the recovery of both mitochondrial and nuclear DNA, with the minimum deterioration for the dental pieces.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Hervella M,Iñiguez MG,Izagirre N,Anta A,de-la-Rúa Cdoi
10.1111/1556-4029.12568subject
Has Abstractpub_date
2015-01-01 00:00:00pages
136-41issue
1eissn
0022-1198issn
1556-4029journal_volume
60pub_type
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