Nondestructive methods for recovery of biological material from human teeth for DNA extraction.

Abstract:

:The extraction of DNA from human skeletal remains applied to forensic, and evolutionary studies do not exclude risks, which are to be evaluated when working with unique specimens that could be damaged or even destroyed. In the present study were evaluated several nondestructive methods for recovering DNA instead of the most currently used pulverization method. Three different procedures to access inside the dental pieces (occlusal perforation, cervical perforation, and cervical cut) have been compared with the aim of recovering as many cell remains as possible to carry out a DNA extraction. Given the DNA quantitation results, a method was proposed that consists of a cervical cut to facilitate the access to the pulp cavity and a subsequent filing of the root canals down to the apex of the dental root. This methodology allows the recovery of both mitochondrial and nuclear DNA, with the minimum deterioration for the dental pieces.

journal_name

J Forensic Sci

authors

Hervella M,Iñiguez MG,Izagirre N,Anta A,de-la-Rúa C

doi

10.1111/1556-4029.12568

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

136-41

issue

1

eissn

0022-1198

issn

1556-4029

journal_volume

60

pub_type

杂志文章
  • Demonstration of esophageal varices postmortem by gastroesophageal phlebography.

    abstract::The study presents a method for the demonstration of esophageal varices at autopsy by gastroesophageal phlebography. The veins visible on the radiographs were controlled and evaluated by studying the gross appearance of sections of the esophagus at the level of the varicose veins and by histologic examination of the s...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Segerberg-Konttinen M

    更新日期:1987-05-01 00:00:00

  • Solid-phase extraction, identification, and quantitation of 11-nor-delta-9-tetrahydrocannabinol-9-carboxylic acid.

    abstract::A procedure has been developed to extract and recover minute amounts of delta-9-carboxytetrahydrocannabinol (THC-COOH) from urine. A new non-isotopic internal standard is introduced to permit a chromatographic assay of the metabolite. The method affords a 91% recovery of 20 ng/mL of the THC-COOH acid from spiked urine...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Nakamura GR,Meeks RD,Stall WJ

    更新日期:1990-07-01 00:00:00

  • Microcrystal analysis of cocaine hydrochloride and added adulterants.

    abstract::The objective of this project was to investigate the trends of changes in the crystal morphology of cocaine in the presence of the common adulterants, caffeine and lidocaine HCl. By performing gold chloride microcrystal tests on samples of cocaine with adulterants at 10, 20, and 50% concentrations, trends in the chang...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2011.01702.x

    authors: Nelson HC,Gardner EA,Matteo D

    更新日期:2011-05-01 00:00:00

  • Decapitation in suicidal hanging--vital reaction patterns.

    abstract::Complete or incomplete decapitation as a consequence of suicidal hanging is very rare, few cases having been reported in the worldwide literature. Posthanging decapitation is typically related to a drop of several meters. Three cases of complete decapitation and one case of incomplete decapitation by suicidal hanging ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12001

    authors: Hejna P,Bohnert M

    更新日期:2013-01-01 00:00:00

  • Assessing the Risk of Secondary Transfer Via Fingerprint Brush Contamination Using Enhanced Sensitivity DNA Analysis Methods.

    abstract::Experiments were performed to determine the extent of cross-contamination of DNA resulting from secondary transfer due to fingerprint brushes used on multiple items of evidence. Analysis of both standard and low copy number (LCN) STR was performed. Two different procedures were used to enhance sensitivity, post-PCR cl...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12911

    authors: Bolivar PA,Tracey M,McCord B

    更新日期:2016-01-01 00:00:00

  • Variation in nuclear DNA concentrations during urination.

    abstract::This study examined the cellular origin and concentration of nuclear DNA in human urine. Ten subjects provided two entire, first-morning voids: one as a single specimen and one as a consecutive series of samples. The serial samples were centrifuged, organically extracted, and quantified by slot-blot analysis. Total DN...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2006.00329.x

    authors: Johnson DJ,Calderaro AC,Roberts KA

    更新日期:2007-01-01 00:00:00

  • Sexing North American Eskimo and Indian innominate bones with the acetabulum/pubis index.

    abstract::The acetabulum/pubis index was tested on American Eskimo and Indian samples of innominate bones to which sex had been assigned by visual inspection. The index alone sexed 91 and 89% of the Eskimo and Indian samples, respectively. Subsequent sorting of misclassified specimens with femur head diameter resulted in 96 and...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Schulter-Ellis FP,Hayek LA

    更新日期:1988-05-01 00:00:00

  • Black soldier fly (Diptera: Stratiomyidae) colonization of pig carrion in south Georgia.

    abstract::The black soldier fly, Hermetia illucens (L.), is thought to colonize corpses 20-30 days postmortem. However, recent observations indicate this might not be true for all cases. Therefore, we conducted a study examining colonization by the black soldier fly and other Diptera on pig carrion in a plowed field in southern...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Tomberlin JK,Sheppard DC,Joyce JA

    更新日期:2005-01-01 00:00:00

  • Suicide by intravenous injection of a veterinary euthanasia agent: report of a case and toxicologic studies.

    abstract::Previously reported cases of suicide by intravenous barbiturates, as well as two other unusual cases [5,6], are compared in Table 2. All decedents were either medical or paramedical personnel and familiar with the drugs and the routes of administration used for their suicides. Lethal is used in veterinary euthanasia a...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Clark MA,Jones JW

    更新日期:1979-10-01 00:00:00

  • Three-dimensional reconstruction of a bullet path: validation by computed radiography.

    abstract::Three-dimensional visualization is an important tool in the evaluation and demonstration of injury. Creating convincing graphics, however, requires strict distinction between illustrative and reconstructive visualizations and a method of validation. We present a case in which we used a radiation-planning tool to provi...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Oliver WR,Chancellor AS,Soltys M,Symon J,Cullip T,Rosenman J,Hellman R,Boxwala A,Gormley W

    更新日期:1995-03-01 00:00:00

  • Fatal falls from height in Taiwan.

    abstract::This study conducts an investigation of fatal falls from height, examines gender differences, and compares our findings with those of Western countries. We review deaths in Taiwan caused by falls from height that underwent forensic autopsy from 1994 to 2010. Among the examined cases, 182 were suicide, 156 were acciden...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12445

    authors: Peng TA,Lee CC,Lin JC,Shun CT,Shaw KP,Weng TI

    更新日期:2014-07-01 00:00:00

  • Matching bullets to bone impact signatures.

    abstract::When bone is penetrated or perforated by a bullet, the bullet's impacting surface is often uniquely modeled by the fractured bone. Reconstructing bone with simple super-imposition of the bullet's contour lines allows matching of a particular bullet to its specific bone defect. ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Smith OC,Symes SA,Berryman HE,LeVaughn MA

    更新日期:1991-11-01 00:00:00

  • Speech watermarking: an approach for the forensic analysis of digital telephonic recordings.

    abstract::In this article, the authors discuss the problem of forensic authentication of digital audio recordings. Although forensic audio has been addressed in several articles, the existing approaches are focused on analog magnetic recordings, which are less prevalent because of the large amount of digital recorders available...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2010.01395.x

    authors: Faundez-Zanuy M,Lucena-Molina JJ,Hagmüller M

    更新日期:2010-07-01 00:00:00

  • Delayed traumatic intracerebral hematoma (DTICH) and the determination of the manner of death. A case report and review of the literature.

    abstract::The concept of a traumatic incident resulting in death when there has been a prolonged interval between the two events raises questions for medical examiners. A case is presented in which a 33-year-old man was found dead in his locked home. Although there was no evidence of foul play at the scene, the death was subseq...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章,评审

    doi:

    authors: McFeeley PJ,Blisard KS

    更新日期:1988-05-01 00:00:00

  • Simultaneous Determination of 13 Anticoagulant Rodenticidesin Human Blood by Liquid Chromatography-Tandem Mass Spectrometry and its Application in Three Poisoning Cases.

    abstract::Anticoagulant rodenticides are widely used for rodent control around the world. A rapid and sensitive method was developed and validated for the simultaneous determination of 13 anticoagulant rodenticides (coumafuryl, pindone, valone, warfarin, coumatetralyl, coumachlor, diphacinone, dicumarol, chlorophacinone, bromad...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.13613

    authors: Qiao Z,Xiang P,Shen B,Shen M,Yan H

    更新日期:2018-05-01 00:00:00

  • Passive Drip Stain Formation Dynamics of Blood onto Hard Surfaces and Comparison with Simple Fluids for Blood Substitute Development and Assessment.

    abstract::The spreading dynamics of blood dripping onto hard surfaces is compared to two spreading models. Samples of human blood, porcine blood, and Millipore® water were dripped onto cardboard, foamcore, and glass surfaces in low velocity passive drip simulations. Final stain diameter, the total number of spines and scallops,...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.13217

    authors: Stotesbury T,Taylor MC,Jermy MC

    更新日期:2017-01-01 00:00:00

  • Bioinformatics Approach to Assess the Biogeographical Patterns of Soil Communities: The Utility for Soil Provenance.

    abstract::Soil DNA profiling has potential as a forensic tool to establish a link between soil collected at a crime scene and soil recovered from a suspect. However, a quantitative measure is needed to investigate the spatial/temporal variability across multiple scales prior to their application in forensic science. In this stu...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.13741

    authors: Damaso N,Mendel J,Mendoza M,von Wettberg EJ,Narasimhan G,Mills D

    更新日期:2018-07-01 00:00:00

  • Investigation of reproducibility and error associated with qPCR methods using Quantifiler® Duo DNA quantification kit.

    abstract::Reproducibility of quantitative PCR results is dependent on the generation of consistent calibration curves via accurate volume transfers and instrument performance. A review of 14 standard curves, using two different QuantDuo® standard DNA lots, showed variability of cycle threshold values between assays were larger ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2010.01460.x

    authors: Grgicak CM,Urban ZM,Cotton RW

    更新日期:2010-09-01 00:00:00

  • Elemental Characterization and Discrimination of Nontoxic Ammunition Using Scanning Electron Microscopy with Energy Dispersive X-Ray Analysis and Principal Components Analysis.

    abstract::Concerns over the toxic by-products produced by traditional ammunition have led to an increase in popularity of nontoxic ammunition. In this work, the chemical composition of six brands of nontoxic ammunition was investigated and compared to that of a road flare, which served as an environmental source with similar co...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12881

    authors: Hogg SR,Hunter BC,Waddell Smith R

    更新日期:2016-01-01 00:00:00

  • Decomposition of buried human bodies and associated death scene materials on coral atolls in the tropical Pacific.

    abstract::The decomposition of bodies and the decay of associated cultural materials, including clothing, personal ornaments, caskets, and grave goods, was investigated based on a series of exhumations on a small sand island on Kwajalein Atoll, Marshall Islands. Decay data were documented for an interment period between 40 and ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Spennemann DH,Franke B

    更新日期:1995-05-01 00:00:00

  • Heteroplasmies detected in an amplified mitochondrial DNA control region from a small amount of template.

    abstract::When mitochondrial DNA (mtDNA) heteroplasmies are detected, they often confound forensic identification, especially if they are the result of poor biological sampling. In this study, we determined the ratio of heteroplasmy in samples that were amplified from a very small amount of template mtDNA or a few cells using a...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2007.00655.x

    authors: Nakahara H,Sekiguchi K,Imaizumi K,Mizuno N,Kasai K

    更新日期:2008-03-01 00:00:00

  • Stability of ecgonine methyl ester in postmortem urine specimens.

    abstract::In this study, 25 postmortem urine specimens testing positive, for cocaine and ecogonine methyl ester (EME) by full scan electron impact gas chromatography/mass spectrometry, were used to evaluate the stability of EME in refrigerated and frozen conditions. After an initial quantitation (t = 0), these specimens were sp...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Levine B,Ramcharitar V,Smialek JE

    更新日期:1996-01-01 00:00:00

  • The Stability of 4-Chloromethcathinone in Blood and Vitreous Humor.

    abstract::We present results of our study on the stability of 4-chloromethcathinone (4-CMC) in authentic postmortem peripheral blood and vitreous humor samples. The stability of 4-CMC was determined in postmortem blood samples (for a period of 90 days) and vitreous humor (30 days) at three different temperatures: -15°C, +4°C, a...

    journal_title:Journal of forensic sciences

    pub_type:

    doi:10.1111/1556-4029.14454

    authors: Nowak K,Szpot P,Zawadzki M

    更新日期:2020-09-01 00:00:00

  • Postmortem clomipramine: therapeutic or toxic concentrations?

    abstract::Postmortem blood and liver concentrations of clomipramine were determined in ten cases by high performance liquid chromatography (HPLC). Blood concentrations ranged from 0.21 to 4.9 mg/L, and liver concentrations from 7.0 to 320 mg/kg. Two cases associated with clomipramine toxicity were clearly differentiated from ot...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: McIntyre IM,King CV,Cordner SM,Drummer OH

    更新日期:1994-03-01 00:00:00

  • Determination of sex of white femora by discriminant function analysis: forensic science applications.

    abstract::Stepwise multiple discriminant function analysis is used to establish classification functions for sex assessment of North American white femora. The functions correctly assign sex for 82% of a sample consisting of 85 femora of verified age, sex, and race, and for a similarly verified test sample of 30. The objectives...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Taylor JV,DiBennardo R

    更新日期:1982-04-01 00:00:00

  • Multivariate Classification of Original and Fake Perfumes by Ion Analysis and Ethanol Content.

    abstract::The increased marketing of fake perfumes has encouraged us to investigate how to identify such products by their chemical characteristics and multivariate analysis. The aim of this study was to present an alternative approach to distinguish original from fake perfumes by means of the investigation of sodium, potassium...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.13070

    authors: Gomes CL,de Lima AC,Loiola AR,da Silva AB,Cândido MC,Nascimento RF

    更新日期:2016-07-01 00:00:00

  • Determination of human immunodeficiency virus antibody status in forensic autopsy cases using a rapid and simple FDA-licensed assay.

    abstract::Evaluation of an FDA-licensed rapid assay to detect antibodies to human immunodeficiency virus (HIV) was accomplished by testing 414 serum samples collected sequentially from autopsy cases at the Office of the Chief Medical Examiner, State of Maryland. The time of postmortem collection ranged from 8 to 30 hours. All s...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Li L,Constantine NT,Zhang X,Smialek JE

    更新日期:1993-07-01 00:00:00

  • Interpreting the effects of burning on pre-incineration saw marks in bone.

    abstract::This study examined the effects of fire on the features associated with saw marks in bone. Both class and individual characteristics were examined using stereomicroscopy and scanning electron microscopy (SEM). Twenty-four semifleshed Sus scrofa L. tibiae were sawed into three sections with the middle section having de...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12580

    authors: Robbins SC,Fairgrieve SI,Oost TS

    更新日期:2015-01-01 00:00:00

  • Spatial and temporal influences on bacterial profiling of forensic soil samples.

    abstract::Bacterial content may be helpful in differentiating forensic soil samples; however, the effectiveness of bacterial profiling depends on several factors, including uniqueness among different habitat types, the level of heterogeneity within a habitat, and changes in bacterial communities over time. To examine these, soi...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2008.00728.x

    authors: Meyers MS,Foran DR

    更新日期:2008-05-01 00:00:00

  • Domestic Homicide: Neuropsychological Profiles of Murderers Who Kill Family Members and Intimate Partners.

    abstract::Domestic homicide is the most extreme form of domestic violence and one of the most common types of homicide. The objective was to examine differences between spontaneous domestic homicide and nondomestic homicide offenders regarding demographics, psychiatric history, crime characteristics, and neuropsychological stat...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12908

    authors: Hanlon RE,Brook M,Demery JA,Cunningham MD

    更新日期:2016-01-01 00:00:00