Abstract：
:Reproducibility of quantitative PCR results is dependent on the generation of consistent calibration curves via accurate volume transfers and instrument performance. A review of 14 standard curves, using two different QuantDuo® standard DNA lots, showed variability of cycle threshold values between assays were larger than those of the Internal PCR Control (IPC). This prompted a set of experiments designed to determine the source of variability. Results showed that error introduced during DNA addition to the plate resulted in little variation. A comparison of seven independent series demonstrated cycle threshold variation between dilutions was larger than the variation expected from repeated samples. Modeling the influence of pipette errors on dilution series accuracy indicated that a more rigorous approach to external calibration curve production is required and showed that improvement in calibration curve stability is expected if the pipette conditions are carefully chosen and ⁄ or a single validated curve is utilized as the calibrator.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Grgicak CM,Urban ZM,Cotton RWdoi
10.1111/j.1556-4029.2010.01460.xsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
1331-9issue
5eissn
0022-1198issn
1556-4029pii
JFO1460journal_volume
55pub_type
杂志文章abstract：:Graphic exemplars of cranial sex and ancestry are essential to forensic anthropology for standardizing casework, training analysts, and communicating group trends. To date, graphic exemplars have comprised hand-drawn sketches, or photographs of individual specimens, which risks bias/subjectivity. Here, we performed qu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13314
更新日期：2017-05-01 00:00:00
abstract：:Microbial biomarkers that indicate aspects of an organism's growth conditions are important targets of forensic research. In this study, we examined fatty acid composition as a signature for the types of complex nutrients in the culturing medium. Bacillus cereus T-strain spores were grown in medium formulations supple...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12771
更新日期：2015-07-01 00:00:00
abstract：:The Tri-State Crematory Incident in Nobel, GA (February 2001) revealed limitations in traditional human cremated remains (cremains) analytical methodology. The goal of this study was to develop a method for effectively classifying questionable sets of cremains as legitimate or contaminated. Eighty-eight samples of kno...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00209.x
更新日期：2006-09-01 00:00:00
abstract：:Drowning as a method of suicide is known to occur, but has primarily been described in environments with readily available access to water, such as coastal regions. In this study, we describe and analyze a series of suicidal drownings occurring in a noncoastal area of Texas. Between 1977 and 1996, 52 cases of suicidal...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:There are no published reports that include both timely antemortem and postmortem carbamazepine concentrations after massive overdose. We report a fatal overdose of carbamazepine with both timely antemortem and postmortem carbamazepine concentrations. Carbamazepine concentrations were 47.7 mcg/mL 2 h antemortem and 53...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:Persons who had been arrested for sexual assault were administered the Luria-Nebraska Neuropsychological Battery and the results compared to a group of normal controls. The sexual assaulters performed significantly worse on 7 of the 14 scales of the battery. The data were then broken down into three groups: (1) those ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Forensic science aims to serve society by advancing justice. It is accepted that some actions taken by the state in the interests of advancing justice, such as postmortem examinations, may impinge on values held by members of groups within society. Such actions have the potential to cause cultural offense. It is impor...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00661.x
更新日期：2008-03-01 00:00:00
abstract：:The estimation of the postmortem interval (PMI) is still one of the most challenging issues in forensic investigations, especially in cases in which advanced transformative phenomena have taken place. The dating of skeletal remains is even more difficult and sometimes only a rough determination of the PMI is possible....
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12024
更新日期：2013-01-01 00:00:00
abstract：:Aortic aneurysms are a common autopsy finding, but aneurysms confined exclusively to the subclavian arteries are rare. When found, they are typically associated with trauma, surgery, or aberrant vessel distribution. Subclavian-esophageal fistula formation is also rare, with the vast majority being related to aberrant ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:Little is known about the racial patterns of crimes committed by sexual homicide offenders (SHOs). This study examined race and age influences on victim-offender relationship for juvenile and adult SHOs. A large sample (N = 3868) from the Supplemental Homicide Reports (1976-2005) was used. Analyses of victim-offender ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01448.x
更新日期：2010-09-01 00:00:00
abstract：:In this paper, we report the results of our preliminary studies into chemical characterization of the fluids produced during decomposition in the absence of a soil matrix. Pig (Sus domestica) carcasses were used to model the human decomposition process in two separate locations, Western Australia (Perth) and Canada (O...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01263.x
更新日期：2010-03-01 00:00:00
abstract：:The record files of the Dade County Medical Examiner Department were reviewed and 133 homicide-suicides were identified. Of these, ten (or 7.5%) were female perpetrated. The chart data of these 10 female homicide-suicide perpetrators, chart data of 50 female homicide-suicide victims, and 50 female individual suicides ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
abstract：:There is an apparent diversity of opinion with respect to the probative value of hair in the United Kingdom and in North America. A questionnaire was devised and widely circulated in an attempt to discover the reasons behind this diversity and to seek the views of a broad range of hair examiners. This paper reports on...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:We herein report the case of a 36-year-old man who died suddenly after a fight with another man. Forensic investigations included unenhanced computed tomography, postmortem angiography, autopsy, histology, neuropathology, toxicology, and biochemistry and allowed a traumatic cause of death to be excluded. An electrocar...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12362
更新日期：2014-05-01 00:00:00
abstract：:Previous attempts to sex juvenile skeletons have focused on the application of qualitative or semi-quantitative techniques. This study applies a variety of geometric morphometric methods, including eigenshape analysis, to this problem. Six metric criteria for the ilia were tested with the aim of investigating previous...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00656.x
更新日期：2008-03-01 00:00:00
abstract：:Electrokinetic injection (EI) is the primary method used in forensic laboratories to load amplified PCR product in capillary electrophoresis for short tandem repeat (STR) fragment separation. Because all samples subjected to capillary electrophoresis use internal lane standard (ILS), this study investigated the conseq...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12945
更新日期：2016-01-01 00:00:00
abstract：:Although the ability to develop latent fingerprints on paper using heat alone has been noted previously, it has been considered impractical for casework and inferior to other techniques. Here a new refinement of the technique is demonstrated for the high quality development of latent fingerprints on porous surfaces su...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00902.x
更新日期：2009-01-01 00:00:00
abstract：:Thromboemboli were found diffusely throughout the pulmonary vasculature system of a 76-year-old white female who died unexpectedly 3 days following injury and repair of a left hip intracapsular fracture. A diffuse chronic nonspecific myocarditis with marked fat atrophy, an acute myocardial infarct of the posterior lef...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Dextromethorphan is a commonly encountered antitussive medication which has found additional therapeutic use in the treatment of pseudobulbar disorder and as an adjunct to opiate use in pain management. Dextromethorphan at high doses has phencyclidine-like effects on the NMDA receptor system; recreational use of high ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2012.02133.x
更新日期：2012-09-01 00:00:00
abstract：:Multishot firearm suicides are relatively rare and suggest the possibility of homicide. Physical activity following gunshots to the head, the neck, and the thorax does occur, and immediate incapacitation does not occur in every fatal gunshot wound that penetrates the head or perforates the heart. Cancer patients appea...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12073
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abstract：:DNA testing of human bones is performed for identification when there is no remaining soft tissue, which often means the samples are old or environmentally compromised. Under these circumstances, it can be difficult to obtain a STR DNA profile. It is important to recover the highest quantity and quality of DNA for STR...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14657
更新日期：2020-12-28 00:00:00
abstract：:The male-specific Y-chromosomal short tandem repeat (STR) is a useful tool in forensic casework. The Y haplotype comprised of 16 loci, which is amplified simultaneously by AmpFlSTR(®) Yfiler(TM) PCR kit and provides strong exculpatory evidence in individual identification. We reported a rare Y-STR profile with a null ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2012.02145.x
更新日期：2012-11-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.13844
更新日期：2019-01-01 00:00:00
abstract：:A standardized removal and dissection procedure is presented for human infant brain. A previously unreported cistern of the pineal gland must be severed at autopsy in order to preserve the gland's anatomic integrity during brain removal. Utilization of these methods to investigate Sudden Infant Death Syndrome brain ti...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:When mitochondrial DNA (mtDNA) heteroplasmies are detected, they often confound forensic identification, especially if they are the result of poor biological sampling. In this study, we determined the ratio of heteroplasmy in samples that were amplified from a very small amount of template mtDNA or a few cells using a...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00655.x
更新日期：2008-03-01 00:00:00
abstract：:This report describes the death of a four-month-old Hispanic male which may be related to benzocaine toxicity. A toxicological evaluation revealed benzocaine at a concentration of 3.48 mg/L, and postmortem methemoglobin of 36% (normal 0.4-1.5). Methemoglobinemia is a complication of benzocaine toxicity. In light of th...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:The investigation of sudden unexpected death in psychiatric patients and the ensuing litigation has brought to our attention many unusual features important in the evaluation of such deaths. Certain pathophysiologic mechanisms of death, rarely encountered in routine forensic science practice, may be important in deter...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:One of the four pillars of the anthropological protocol is the estimation of sex. The protocol generally consists of linear metric analysis or visually assessing individual skeletal traits on the skull and pelvis based on an ordinal scale of 1-5, ranging from very masculine to very feminine. The morphologic traits are...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00627.x
更新日期：2008-01-01 00:00:00
abstract：:Electrostatic dust print lift method is known to be able to recover only dry-origin footwear impression. However, the wet-origin footwear impression could also be recovered using this method. As the amount of dust accumulated before deposition of the wet-origin footwear impression increased, the intensity of the footw...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13743
更新日期：2018-09-01 00:00:00
abstract：:Malingering by proxy (MAL-BP) is a form of maltreatment that involves a caregiver who fabricates or induces signs or symptoms in a child, dependent adult, or pet in pursuit of external, tangible incentives. Rarely studied, MAL-BP has an unknown prevalence, and is a challenging diagnosis for healthcare professionals. T...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12977
更新日期：2016-01-01 00:00:00
