Abstract：
:Reducing amplicon sizes has become a major strategy for analyzing degraded DNA typical of forensic samples. However, amplicon sizes in current mini-short tandem repeat-polymerase chain reaction (PCR) and mini-sequencing assays are still not suitable for analysis of severely degraded DNA. In this study, we present a multiplex typing method that couples ligase detection reaction with PCR that can be used to identify single nucleotide polymorphisms and small-scale insertion/deletions in a sample of severely fragmented DNA. This method adopts thermostable ligation for allele discrimination and subsequent PCR for signal enhancement. In this study, four polymorphic loci were used to assess the ability of this technique to discriminate alleles in an artificially degraded sample of DNA with fragment sizes <100 bp. Our results showed clear allelic discrimination of single or multiple loci, suggesting that this method might aid in the analysis of extremely degraded samples in which allelic drop out of larger fragments is observed.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Zhang Z,Wang BJ,Guan HY,Pang H,Xuan JFdoi
10.1111/j.1556-4029.2009.01166.xsubject
Has Abstractpub_date
2009-11-01 00:00:00pages
1304-9issue
6eissn
0022-1198issn
1556-4029pii
JFO1166journal_volume
54pub_type
杂志文章abstract：:The short tandem repeat systems (STRs) HumvWA, HumFXIIIB, and HumFES/FPS were amplified in a triplex polymerase chain reaction (PCR) on blood samples from 100 unrelated Yemenians and 100 unrelated Egyptians. The samples were analyzed by native horizontal discontinual electrophoresis. No deviations from Hardy-Weinberg ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:An analytical procedure for the simultaneous determination of both cocaine and benzoylecgonine in postmortem fluid and tissue samples has been developed by using computerized gas chromatography/mass spectrometry and gas chromatography using a nitrogen/phosphorus (N/P) detector. Both methods are accurate and sensitive ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:Vitreous humor chemistry profiles were reviewed on 133 autopsied cases in which death occurred outdoors during a six-year period, to determine whether environmental temperature at the time of death influenced chemistry values obtained at autopsy. The glucose concentration and total carbon dioxide content varied invers...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:The objective of this study was to compare the accuracy of contact and laser 3D scanners in tooth mark analysis. Ten dental casts were scanned with both 3D scanners. Seven linear measurements were made from the 3D images of dental casts and biting edges generated with DentalPrint© software (University of Granada, Gran...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12598
更新日期：2015-01-01 00:00:00
abstract：:A population study on the distribution of alleles for the D1S80 locus (pMCT118) was carried out on 141 unrelated and healthy blood donors from the province of Messina (Eastern Sicily). Forty-two different genotypes and 16 different alleles were observed and two of these were found to be relatively common in the sample...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Regional studies that examine decomposition rates of certain faunal remains can help to determine time since death. Forensic anthropologists have long used qualitative decomposition data, but linking these to more quantitative data could improve time since death estimations. Experiments were developed in which domesti...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:Many current methods of age determination available to forensic anthropologists are limiting in that the age ranges provided are often broad, particularly for individuals in their late teens to early 20's. This study introduces an improved method for aging teenagers and young adults. The pattern and stages of union of...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:Positive identification of human remains is often achieved by comparing antemortem and postmortem radiographs. Usually, radiographs contain a number of markers that can serve as reference for comparison, one of these markers is the trabecular bone pattern depicted in the roentgenograms. In the present case, densitomet...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:A procedure for the multisystem analysis of bloodstains using the simultaneous separation of the enzymes glyoxalase I, esterase D, and phosphoglucomutase has been developed. The amount of bloodstain required has therefore been reduced threefold without any loss in resolution and sensitivity. Bloodstains at least seven...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Aortic aneurysms are a common autopsy finding, but aneurysms confined exclusively to the subclavian arteries are rare. When found, they are typically associated with trauma, surgery, or aberrant vessel distribution. Subclavian-esophageal fistula formation is also rare, with the vast majority being related to aberrant ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:Gunshot backspatter comprises biological material expelled backward through bullet entry holes. Crime scene investigators analyze backspatter patterns to infer wounding circumstances. An understanding of the mechanism of backspatter generation, and the relationship between spatter patterns and bullet and tissue charac...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2012.02143.x
更新日期：2012-09-01 00:00:00
abstract：:Zip guns and the atypical gunshot wounds they produce are rare in forensic pathology. Because of this, investigators and forensic pathologists may be unfamiliar with their construction, appearance, and the wounds associated with them. A 43-year-old mechanic, with a history of depression was found dead in a washroom st...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00621.x
更新日期：2008-01-01 00:00:00
abstract：:Domestic homicide is the most extreme form of domestic violence and one of the most common types of homicide. The objective was to examine differences between spontaneous domestic homicide and nondomestic homicide offenders regarding demographics, psychiatric history, crime characteristics, and neuropsychological stat...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12908
更新日期：2016-01-01 00:00:00
abstract：:During postmortem examination of the dental arches of carbonized victims, dental restorative materials may be found. The aim of this study was to evaluate the effect of heat source action on the color stability of composite resin (CR) and glass ionomer cement (GIC) restorations, to discriminate between them and compar...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13292
更新日期：2017-03-01 00:00:00
abstract：:Unsupported excised skin may shrink by as much as 50% or more. In 1981, a method was developed for ring adhesion to skin with the goal of minimizing tissue distortion upon excision. Five modified versions of the technique bearing the author's name followed (Dorion types I, II, III, IV, and V). The scientific literatur...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01604.x
更新日期：2011-01-01 00:00:00
abstract：:In this study, the nondestructive differentiation of individual white polyester clothing fibers was accomplished via synchrotron radiation microbeam X-ray fluorescence (SR-μ-XRF) analysis. SR-μ-XRF with vertical focusing is a useful nondestructive method for the analysis of a single polyester clothing fiber. Kirkpatri...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14481
更新日期：2020-09-01 00:00:00
abstract：:Identification of sex from the skeleton is an important demographic assessment in medicolegal investigations. Studies have demonstrated that populations differ from each other in size and proportions and that these differences can affect the metric assessment of sex. It is therefore vital to determine if population di...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Human erythrocyte peptidase A (Pep A) displays a genetic polymorphism in blacks. Its occurrence in human semen was examined for its possible use as a semen typing system. Studies by starch gel electrophoresis, in which the Pep A was located by an improved method, were carried out on semen, semen stains, and vaginal sw...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:This is the first report regarding the characterization of the new synthetic cannabinoid 4F-MDMB-BINACA. 4F-MDMB-BINACA was first analytically confirmed in seized drug material using gas chromatography-mass spectrometry (GC-MS), liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF), and nuclear m...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14101
更新日期：2019-09-01 00:00:00
abstract：:When insect evidence is obtained during autopsy, forensic entomologists make decisions regarding the effects of low-temperature (-1 degrees C to 4 degrees C) storage of the body and associated insects when estimating the post-mortem interval (PMI). To determine the effects of storage in a morgue cooler on the temperat...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00385.x
更新日期：2007-03-01 00:00:00
abstract：:Human ecchinococcosis also known as hydatid disease is a zoonotic infection caused by the tapeworm Ecchinococcus with 2-3 Million cases worldwide. We hereby report a 6 years period study of Sudden death due to hydatidosis aiming to analyze the epidemiological criteria, death circumstances, and autopsy observations att...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12172
更新日期：2013-09-01 00:00:00
abstract：:Many epidemiological, clinical, and autopsy studies have demonstrated that the risk of sudden coronary death (SCD) is linked to the severity of coronary atherosclerotic lesions and to their thrombotic potential. However it remains unknown why some individuals manifest no clinical evidence of coronary disease until the...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:Progress in the understanding of individuals who commit arson has been on a slow but steady course over the past two decades. From our review of court-ordered outpatient forensic psychiatric evaluation of individuals charged with arson over a five-year period, preliminary prototypical profiles of the psychotic, mental...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:While victims of racially motivated violence may be identified through observation of morphological features, those targeted because of their ethnic, religious, or national identity are not easily recognized. This study examines how perpetrators of genocide recognize their victims. Court documents, including indictmen...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00590.x
更新日期：2008-01-01 00:00:00
abstract：:An unusual fatality involving a 26-year-old male who intentionally ingested approximately 10 g of metoprolol is presented. Autopsy findings revealed foreign material in the gastric content with an acute thrombosis of the left anterior descending coronary artery. Microscopic studies showed evidence of an extremely earl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：
更新日期：1987-09-01 00:00:00
abstract：:Autoerotic deaths are normally classified as accidental deaths occurring during individual, and usually solitary, sexual activity in which a device, apparatus, or prop used to enhance sexual stimulation of the deceased somehow causes unintended death. The most common methods of autoerotic practices are asphyxia by han...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00496.x
更新日期：2007-07-01 00:00:00
abstract：:The term Aqua-eroticum was first introduced in 1984 by Sivaloganathan to describe the unusual autoerotic death of a man using submersion as an asphyxia method. This was the first case of that kind, and since then, no other case of autoerotic submersion has been reported, nor other autoerotic fatality in open water. He...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2005.00031.x
更新日期：2006-01-01 00:00:00
abstract：:This paper has reported a case of sudden unexpected death in a 19-year-old man, shot in the abdomen, who was surgically treated and discharged. He died of massive left retropleural hematoma from a spontaneous rupture of a traumatic pseudo-aneurysm of the abdominal aorta. Diagnosis of the retropleural hemorrhage and th...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1979-07-01 00:00:00
abstract：:A case of apparent suicide by injection of snake venom was investigated. Evidence found at the scene and presumably used for self-injection was positive for nonspecific venom but had the characteristics of a viper venom. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:Data from 100 cases of alleged rape victims demonstrate why the author believes the AP test to be a reliable tool, when employed by an expert for the identification of seminal fluid. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
