Abstract：
:Electrokinetic injection (EI) is the primary method used in forensic laboratories to load amplified PCR product in capillary electrophoresis for short tandem repeat (STR) fragment separation. Because all samples subjected to capillary electrophoresis use internal lane standard (ILS), this study investigated the consequence of varying the volume of ILS and its effects on allele peak heights and number of alleles detected. Results demonstrated that when the volume of ILS is reduced, the average peak height and number of alleles increased, thereby increasing the sensitivity of the detection method. Sizing anomalies were observed; however, they did not adversely affect accuracy and precision. The method developed in this study offers a simple and universal procedure to increase the alleles detected in forensic STR analysis. Reducing the volume of ILS to achieve greater sensitivity is applicable to all STR amplification kits and capillary electrophoresis instruments currently used in forensic DNA analysis.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Esparza JM,Michalik M,Dukes MJ,Wojtkiewicz Pdoi
10.1111/1556-4029.12945subject
Has Abstractpub_date
2016-01-01 00:00:00pages
S213-20eissn
0022-1198issn
1556-4029journal_volume
61 Suppl 1pub_type
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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