Abstract：
:This study describes the testing performed by the Minnesota Forensic Science Laboratory (MFSL) to validate the Amplitype DQA1 and Amplitype Polymarker (PM) PCR Amplification and Typing Kits before implementation for casework. All studies were based on the analysis of mock forensic case samples, which were assembled from various biological samples from individuals at the MFSL. To address the validation of standard specimens, DNA was isolated from semen, vaginal secretions, saliva, urine, and blood samples. Typing results from all tissues from a particular individual yielded the same typing results using both the DQA1 and PM systems. Reproducibility between laboratories was evaluated by having duplicate samples analyzed by a second laboratory. The Roche Biomedical Laboratories (RBL) were sent a duplicate set of mock cases and all analyses including extraction, quantitation, amplification, and typing were performed at the RBL using their established testing procedures. All typing results for both laboratories, from the approximate 30 single source samples analyzed, were in agreement. Mixed specimens were evaluated by examining the results obtained from semen/vaginal, semen/saliva, semen/blood, semen/ urine, and semen/vaginal/blood mixtures. All typing results of these mixtures for both laboratories were in agreement. It was determined that by incorporating a wash step of the sperm cell pellet, a complete separation of the nonsperm cell fraction was more likely to be attained. After completing the above studies, as well as population studies, environmental insult studies, and proficiency testing, the MFSL determined that both kits were suitable for use on forensic casework.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Gross AM,Guerrieri RAsubject
Has Abstractpub_date
1996-11-01 00:00:00pages
1022-6issue
6eissn
0022-1198issn
1556-4029journal_volume
41pub_type
杂志文章abstract：:Skeletal remains were discovered in an unused cistern near the Omaha, Nebraska airport and were positively identified through comparison of antemortem and postmortem dental radiographs. Although nearly nine years had elapsed between death and discovery, the bones and teeth revealed evidence of the application of a cor...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:In this paper, a novel three-dimensional transformation method for vehicle license plate number recognition is proposed. This method provides an efficient solution to normalize skew distorted vehicle license plate images. The Hough transform is used to estimate the license plate position and the normalization angle. A...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:This paper examines complications of sleepwalking disorder (DSM-IV 307.46), an arousal disorder or parasomnia, in relationship to mens rea, or culpable mental state necessary to a finding of criminal responsibility. The legal history of criminal intent and insanity is reviewed. A case of indecent exposure is discussed...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：POPULATION:Identifiler-Employees and students of Muhimibili University College of Health Sciences in Dar es Salaam representing 19 widely distributed administrative districts and 42 tribes within the country. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00640.x
更新日期：2008-01-01 00:00:00
abstract：:The recent decline in the incidence of the SIDS in the District of Columbia has permitted identification of what appears to be two separate groups of cases within the generic SIDS population. While deaths of bed-sharing victims remained relatively constant throughout the study period, a greater than fourfold decrease ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：:Animal-inflicted injuries to humans are a major public health problem around the world resulting in great morbidity, money loss, and mortality. They are related to wild and domestic animals alike. Animals can cause injuries by various mechanisms--biting, stinging, crushing, goring, stomping, butting, kicking, pecking,...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12813
更新日期：2015-09-01 00:00:00
abstract：:The third edition of the Diagnostic and Statistical Manual of the American Psychiatric Association (DSM-III) is evaluated from the standpoint of forensic science experience in a family court setting. The importance of diagnosis in developing pertinent recommendations within an adversarial system is discussed, with par...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:We present an unusual example of multiple mesosternal foramina (MMF). The alignment of the paired defects is unlike any previously described. Although single sternal defects are often encountered, paired defects are quite uncommon. This is the first documented example of bilateral paired defects in the sternum. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:This study evaluated the reliability of Widmark calculations, based on breath ethanol reading (BrACs), for estimating the amount of alcohol consumed. A standard ethanol dose (males 0.51 g/kg; females 0.43 g/kg) was given to 115 college seniors, and BrACs were measured for two hours. Calculations of ethanol dose were p...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Recent publications from the National Academy of Sciences have called for additional foundational research in the field of firearm and toolmark analysis. We examined test fires from 10 pistol slides with consecutively manufactured breech faces. A total of nine test fires from each pistol slide, for a total of 90 test ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2012.02072.x
更新日期：2012-07-01 00:00:00
abstract：:A sample of 31 criminal defendants who were charged with murder but without major mental disorder was studied. The sample was derived from a large urban multi-ethnic, multi-cultural community pool. Subject characteristics and information about the homicide and decedents are described. Suggestions for further study are...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:The dramatic increase in the sensitivity of DNA profiling systems that has occurred over recent years has led to the need to address a wider range of interpretational problems in forensic science. The issues surrounding questions of the kind "whose DNA is this?" have been the subject of considerable controversy but no...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:This paper reports on the disaster that occurred after a flight was hijacked at Baiyuen International Airport in Guangzhou, China, on Tuesday, 2 Oct. 1990. The authors show the results of analyses for the causes of death and trauma and compare the disaster with several previous flight accidents that have occurred in C...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Air embolism may rarely cause sudden death during or shortly after pregnancy. Certain obstetric techniques, douching procedures, and blowing air into the vagina have been associated with this phenomenon. A case of venous air embolism in a pregnant woman occurring during sexual intercourse in a rear entry position is p...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:We describe a massive fatal posterior myocardial infarct resulting from compression of the right coronary artery by a Teflon pledget granuloma in a patient who had undergone aortic valve replacement six months before death. Other iatrogenic causes of coronary artery occlusion or compression are discussed in this paper...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Opium is the raw material for the production of heroin, and the characterization of opium seizures through laboratory analysis is a valuable tool for law enforcement agencies to trace clandestine opium production and trafficking. In this work, a method for opium profiling based on the relative content of five principa...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13190
更新日期：2016-11-01 00:00:00
abstract：:Molecular-based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extra...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14070
更新日期：2019-11-01 00:00:00
abstract：:Diethyl ether was found in samples of the blood, urine, and lung from a fire victim. Because the lung also carried a number of low-boiling paraffins, it was inferred that the detected compounds came from engine starting fluid, which the victim apparently had been exposed to while be intentionally used it as an acceler...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:When insect evidence is obtained during autopsy, forensic entomologists make decisions regarding the effects of low-temperature (-1 degrees C to 4 degrees C) storage of the body and associated insects when estimating the post-mortem interval (PMI). To determine the effects of storage in a morgue cooler on the temperat...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00385.x
更新日期：2007-03-01 00:00:00
abstract：:The aim of this study was to assess the histomorphological features of the fourth rib and to develop age-predicting equations for Koreans. Sixty-four rib samples (36 males and 28 females) obtained from forensic cases were used for developing equations. Two thin sections (<100-microm thick) per sample were prepared by ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00566.x
更新日期：2007-11-01 00:00:00
abstract：:Clinicians tend to overestimate their ability to recognize feigning behavior in psychiatric patients, especially if it concerns patients who have been admitted for observation. Feigning can be either externally motivated (e.g., for financial compensation, known as malingering) or internally motivated (e.g., to assume ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14320
更新日期：2020-07-01 00:00:00
abstract：:While victims of racially motivated violence may be identified through observation of morphological features, those targeted because of their ethnic, religious, or national identity are not easily recognized. This study examines how perpetrators of genocide recognize their victims. Court documents, including indictmen...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00590.x
更新日期：2008-01-01 00:00:00
abstract：:Borate-containing compounds were formerly used as topical antiseptics and were components of many medicinal preparations including skin powders and ointments used for the treatment of burns and diaper rash. These compounds were also used as irrigants for body cavities, including the pleural, vaginal, and rectal caviti...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00420.x
更新日期：2007-05-01 00:00:00
abstract：:A case of multiple animal bite wounds is presented along with the techniques used to apprehend the culprits. The victim was a 13 month-old black female who had in excess of 80 puncture wounds. Initially the wounds were thought to be caused by a mechanical instrument but, after an autopsy, were determined to be caused ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1975-04-01 00:00:00
abstract：:Decomposition studies were conducted to determine differences in rates and patterns of decomposition of carcasses hanging and exposed on the surface of the soil. These studies were conducted between 17 October and 17 December 1997 inside of Diamond Head Crater on the island of Oahu, Hawaii. The animal model was the do...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:A forensic procedure for the quantitative confirmation of lysergic acid diethylamide (LSD) and the qualitative confirmation of its metabolite, N-demethyl-LSD, in blood, serum, plasma, and urine samples is presented. The Zymark RapidTrace was used to perform fully automated solid-phase extractions of all specimen types...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:In order to establish an animal model for entomotoxicological studies, the kinetics of morphine elimination from blood after a single intravenous injection of morphine and the concentration of morphine in tissues following a continuous perfusion were studied. The aim of these experiments was to obtain controlled morph...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Field studies in forensic entomology seem to assume that a cadaver is a resource equally accessible to flies, irrespective of the species' size, behavior, and sensorial capacity. We aimed to challenge this assumption by experimentally manipulating baited traps in order to test whether the accessibility to decomposing ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14328
更新日期：2020-09-01 00:00:00
abstract：:Deoxyribonucleic acid (DNA) samples extracted from stained pap smear, semen smear and postcoital slides were amplified by the polymerase chain reaction (PCR) and typed for the AmpliType PM and HLA DQ alpha alleles. HLA DQ alpha and PM types consistent with blood controls from the donors were obtained. Stained cells fi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:The "Blue Whale Challenge" is a dangerous Internet phenomenon. As per media reports, it involves a series of life-threatening tasks imposed by a "curator" to "players," who must fulfill the whole list, and it ends with the suicide of the player. The authors report the data of five suspected cases of "Blue Whales" mana...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：10.1111/1556-4029.13880
更新日期：2019-03-01 00:00:00
