Abstract：
:Molecular-based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extraction, requiring an additional step in the forensic analysis workflow. The purpose of this study was to evaluate miRNA detection in blood, semen, and saliva using DNA extraction methods commonly utilized for forensic casework. RT-qPCR analysis revealed that the tested miRNAs were consistently detectable across most tested DNA extraction methods, but detection was significantly reduced compared to RNA extracts in some biological fluids. DNase treatment was not necessary to achieve miRNA-specific results. A previously developed miRNA panel for forensic body fluid identification was evaluated using DNA extracts, and largely demonstrated concordance with results from samples deriving from RNA extracts of semen, blood, and saliva.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Lewis CA,Layne TR,Seashols-Williams SJdoi
10.1111/1556-4029.14070subject
Has Abstractpub_date
2019-11-01 00:00:00pages
1823-1830issue
6eissn
0022-1198issn
1556-4029journal_volume
64pub_type
杂志文章abstract：:The smokeless powders in 22 kinds of ammunitions seized from one of the Japanese gang groups were analyzed by scanning electron microscopy/energy dispersive X-ray microanalysis (SEM/EDX). Copper(Cu), sulfur(S), potassium(K), silicon(Si), aluminum(Al), calcium(Ca), iron(Fe), chlorine(Cl), and barium(Ba) were detected. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Progress in the understanding of individuals who commit arson has been on a slow but steady course over the past two decades. From our review of court-ordered outpatient forensic psychiatric evaluation of individuals charged with arson over a five-year period, preliminary prototypical profiles of the psychotic, mental...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:Air embolism may rarely cause sudden death during or shortly after pregnancy. Certain obstetric techniques, douching procedures, and blowing air into the vagina have been associated with this phenomenon. A case of venous air embolism in a pregnant woman occurring during sexual intercourse in a rear entry position is p...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:The combination of photographs taken at two or three wavelengths at and bracketing an absorbance peak indicative of a particular compound can lead to an image with enhanced visualization of the compound. This procedure works best for compounds with absorbance bands that are narrow compared with "average" chromophores....
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:Gabapentin, a GABA analogue, is a nonopioid prescribed for seizure control and neuralgic pain. Its abuse for recreational purposes has been increasing in recent years as the number of prescriptions also increases. In our series, we review 104 cases of decedents who tested positive for gabapentin in postmortem blood sa...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14021
更新日期：2019-07-01 00:00:00
abstract：:Genipin, a hydrolytic product of geniposide extracted from gardenia fruit, was thoroughly studied as a potential fingerprint reagent, and optimal conditions for fingerprint development have been determined. Latent fingerprints on paper items that have been treated with a non-ink running formulation containing 0.17% of...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:New procedures, tailored after such court decisions as Rogers v. Commissioner of the Department of Mental Health, have restricted the doctor's ability to treat psychiatric inpatients with psychotropic medication and have increased the protection of a competent patient's right to refuse. This study investigates how the...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Low explosives such as smokeless powder, black powder, and black powder substitutes have been used in illicit pipe bombings throughout the United States. Some of the newer black powder substitutes are formulated with ascorbic acid, which gradually decomposes as the powder ages, making it difficult if not impossible fo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:Age at death assessment of adult skeletons is one of the most difficult problems in forensic and physical anthropology. Two fundamental sources of error are described: the complex variability in the process of skeletal aging and methodological bias. Taking into account these limits, we developed a new scoring system f...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:The third edition of the Diagnostic and Statistical Manual of the American Psychiatric Association (DSM-III) is evaluated from the standpoint of forensic science experience in a family court setting. The importance of diagnosis in developing pertinent recommendations within an adversarial system is discussed, with par...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Recent court challenges have highlighted the need for statistical research on fingerprint identification. This paper proposes a model for computing likelihood ratios (LRs) to assess the evidential value of comparisons with any number of minutiae. The model considers minutiae type, direction and relative spatial relati...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00327.x
更新日期：2007-01-01 00:00:00
abstract：:Compositional analysis of the organic additives in smokeless handgun powder can provide forensic information to associate known and questioned samples. A reliable method for the quantitative extraction of smokeless powder additives would strengthen these measurements. To achieve quantitative recovery, both supercritic...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:Agencies all around the world now use less-lethal weapons with homogeneous missiles such as bean bag or rubber bullets. Contusions and sometimes significant morbidity have been reported. This study focuses on wounds caused by hybrid ammunition with the pathologists' flap-by-flap procedure. Twenty-four postmortem human...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01431.x
更新日期：2010-09-01 00:00:00
abstract：:Visualization of nitrite residues is essential in gunshot distance determination. Current protocols for the detection of nitrites include, among other tests, the Modified Griess Test (MGT). This method is limited as nitrite residues are unstable in the environment and limited to partially burned gunpowder. Previous re...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13802
更新日期：2019-01-01 00:00:00
abstract：:The short tandem repeat systems (STRs) HumvWA, HumFXIIIB, and HumFES/FPS were amplified in a triplex polymerase chain reaction (PCR) on blood samples from 100 unrelated Yemenians and 100 unrelated Egyptians. The samples were analyzed by native horizontal discontinual electrophoresis. No deviations from Hardy-Weinberg ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Multishot firearm suicides are relatively rare and suggest the possibility of homicide. Physical activity following gunshots to the head, the neck, and the thorax does occur, and immediate incapacitation does not occur in every fatal gunshot wound that penetrates the head or perforates the heart. Cancer patients appea...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12073
更新日期：2013-03-01 00:00:00
abstract：:Age-at-death estimations of 44 individuals (27 adults, 17 children) from the Branch Davidian sample were compared with their actual ages. Estimations were evaluated for bias and accuracy for the actual age at death. Although the overall average estimates correlated well with the actual ages (r = 0.946), several indivi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:Soil DNA profiling has potential as a forensic tool to establish a link between soil collected at a crime scene and soil recovered from a suspect. However, a quantitative measure is needed to investigate the spatial/temporal variability across multiple scales prior to their application in forensic science. In this stu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13741
更新日期：2018-07-01 00:00:00
abstract：:Concerns have been raised about hypothetical problems arising from the use of statistics for determining the likelihood of occurrence of DNA profiles for forensic purposes. A major contention is that reference databases based on subgroups of a major population category rather than on general (or major) population grou...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:The record files of the Dade County Medical Examiner Department were reviewed and 133 homicide-suicides were identified. Of these, ten (or 7.5%) were female perpetrated. The chart data of these 10 female homicide-suicide perpetrators, chart data of 50 female homicide-suicide victims, and 50 female individual suicides ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
abstract：:Advances in the technology employed for the manufacture of glass have resulted in a final glass product with little variability in terms of its physical and optical properties. For example, the refractive index of Australian float glass tends to lie between 1.5189 and 1.5194. It has therefore become necessary to compl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00644.x
更新日期：2008-03-01 00:00:00
abstract：:Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is comp...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:A case is presented where phentermine, a sympathomimetic amine used as an anorectic drug, is believed to have significantly contributed to the death of an individual in whom other drugs were also found. Blood, urine, and tissue concentrations of phentermine are reported and compared to other cases in the literature. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Using postmortem multislice computed tomography (MSCT) and magnetic resonance imaging (MRI), 40 forensic cases were examined and findings were verified by subsequent autopsy. Results were classified as follows: (I) cause of death, (II) relevant traumatological and pathological findings, (III) vital reactions, (IV) rec...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:This study analyses depressed fractures (by blunt force trauma) and circular full-thickness injuries (drill injuries and gunshot wounds) in charred bones. Fifty bovine ribs (total 104 lesions) were divided into three groups. The first group consisted in 20 depressed hammer-produced fractures; in the second one, 60 rou...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12794
更新日期：2015-09-01 00:00:00
abstract：:Powder stippling caused by the impact of propellant particles during close-distance gunfire has been previously described on skin and solid objects only. Additionally, radial tearing has been described as clear evidence of a contact-distance shot, requiring no further testing. Patterns of discrete perforating holes (r...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13619
更新日期：2018-05-01 00:00:00
abstract：:Although the ability to develop latent fingerprints on paper using heat alone has been noted previously, it has been considered impractical for casework and inferior to other techniques. Here a new refinement of the technique is demonstrated for the high quality development of latent fingerprints on porous surfaces su...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00902.x
更新日期：2009-01-01 00:00:00
abstract：:At a recent bushland gravesite that contained a deceased male, a clod of soil displaying a striated toolmark was collected during the excavation of the grave. This clod was preserved, and the mark was cast using Mikrosil™. Shovels and a mattock, which had been discarded by the suspects, were subsequently found at anot...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01537.x
更新日期：2011-01-01 00:00:00
abstract：:Nosocomial infections have become a major issue of public health and lead to an increasing number of suits for damages. We present a rare case of Aspergillus contamination during cardiac surgery, describe the medicolegal investigation, and present the new system for compensation of bodily injury after nosocomial infec...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.13328
更新日期：2017-05-01 00:00:00
abstract：:Forensic science aims to serve society by advancing justice. It is accepted that some actions taken by the state in the interests of advancing justice, such as postmortem examinations, may impinge on values held by members of groups within society. Such actions have the potential to cause cultural offense. It is impor...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00661.x
更新日期：2008-03-01 00:00:00
