Detection of microRNAs in DNA Extractions for Forensic Biological Source Identification.

Abstract:

:Molecular-based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extraction, requiring an additional step in the forensic analysis workflow. The purpose of this study was to evaluate miRNA detection in blood, semen, and saliva using DNA extraction methods commonly utilized for forensic casework. RT-qPCR analysis revealed that the tested miRNAs were consistently detectable across most tested DNA extraction methods, but detection was significantly reduced compared to RNA extracts in some biological fluids. DNase treatment was not necessary to achieve miRNA-specific results. A previously developed miRNA panel for forensic body fluid identification was evaluated using DNA extracts, and largely demonstrated concordance with results from samples deriving from RNA extracts of semen, blood, and saliva.

journal_name

J Forensic Sci

authors

Lewis CA,Layne TR,Seashols-Williams SJ

doi

10.1111/1556-4029.14070

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

1823-1830

issue

6

eissn

0022-1198

issn

1556-4029

journal_volume

64

pub_type

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