Comparative analysis of the effects of heat on the PCR-amplification of various sized DNA fragments extracted from Sus Scrofa molars.

Abstract:

:This study examined the effects of heat on the amplification of DNA from the dental pulp of Sus scrofa molars and investigated the protection afforded to the pulp tissue by the dental enamel, alveolar process, and soft tissue of the head. Segments of defleshed maxilla and mandible encasing the first molar (n = 60) were subject to a range of temperatures for 15 min. Dental pulps were retrieved. Amplifications using three-primer and four-primer multiplexes showed no degradation of the largest fragment following exposure to 450 degrees C. Amplifications in the three-primer multiplex (283 bp) were successful following exposure to 525 degrees C in maxillary samples only. This study revealed the enamel density of maxillary molars to be greater than mandibular molars in Sus scrofa. Following incineration of intact heads for 15 min (n = 10) and 1 h (n = 4) at an average temperature of 625 degrees C, amplifications of the largest fragment (450 bp) were successful from both maxillary and mandibular teeth.

journal_name

J Forensic Sci

authors

Rees KA,Cox MJ

doi

10.1111/j.1556-4029.2009.01286.x

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

410-7

issue

2

eissn

0022-1198

issn

1556-4029

pii

JFO1286

journal_volume

55

pub_type

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