Combined DNA Typing and Protein Identification from Unfired Brass Cartridges,,.

Abstract:

:Biological evidence analysis from contact traces is adversely affected by low quantity and quality of DNA. Proteins in these samples contain potentially individualizing information and may be particularly important for difficult surfaces such as brass, where DNA may yield incomplete profiles. In this study, touched unfired brass cartridges were sampled using dry tape or wet swabs and analyzed by separating DNA and protein from the same collected material, thus producing both genomic and proteomic information. DNA recovery was similar for both collection methods, with tape yielding an average of 1.36 ± 1.87 ng and swabs, 1.34 ± 3.04 ng. Analysis by mass spectrometry identified 95 proteins, with the two collection methods showing no significant difference (p = 0.76) in the average number of collected proteins: 44.5 ± 10.9, (tape) versus 47.9 ± 20.4 (swabs). Proteins can be collected from fingerprints at levels necessary to provide identifying information, thus expanding information obtained from challenging evidence.

journal_name

J Forensic Sci

authors

Sterling SA,Mason KE,Anex DS,Parker GJ,Hart B,Prinz M

doi

10.1111/1556-4029.14042

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

1475-1481

issue

5

eissn

0022-1198

issn

1556-4029

journal_volume

64

pub_type

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