Abstract:
:Evidence from many organisms indicates that the conserved RecQ helicases function in the maintenance of genomic stability. Mutation of SGS1 and WRN, which encode RecQ homologues in budding yeast and humans, respectively, results in phenotypes characteristic of premature aging. Mutation of SRS2, another DNA helicase, causes synthetic slow growth in an sgs1 background. In this work, we demonstrate that srs2 mutants have a shortened life span similar to sgs1 mutants. Further dissection of the sgs1 and srs2 survival curves reveals two distinct phenomena. A majority of sgs1 and srs2 cells stops dividing stochastically as large-budded cells. This mitotic cell cycle arrest is age independent and requires the RAD9-dependent DNA damage checkpoint. Late-generation sgs1 and srs2 cells senesce due to apparent premature aging, most likely involving the accumulation of extrachromosomal rDNA circles. Double sgs1 srs2 mutants are viable but have a high stochastic rate of terminal G2/M arrest. This arrest can be suppressed by mutations in RAD51, RAD52, and RAD57, suggesting that the cell cycle defect in sgs1 srs2 mutants results from inappropriate homologous recombination. Finally, mutation of RAD1 or RAD50 exacerbates the growth defect of sgs1 srs2 cells, indicating that sgs1 srs2 mutants may utilize single-strand annealing as an alternative repair pathway.
journal_name
Geneticsjournal_title
Geneticsauthors
McVey M,Kaeberlein M,Tissenbaum HA,Guarente Lkeywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
1531-42issue
4eissn
0016-6731issn
1943-2631journal_volume
157pub_type
杂志文章相关文献
GENETICS文献大全abstract::Hybrid sterility in the heterogametic sex is a common feature of speciation in animals. In house mice, the contribution of the Mus musculus musculus X chromosome to hybrid male sterility is large. It is not known, however, whether F1 male sterility is caused by X-Y or X-autosome incompatibilities or a combination of b...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.141804
更新日期:2012-08-01 00:00:00
abstract::The maize r locus encodes a transcription factor that regulates the developmental expression of the plant pigment anthocyanin. In an unusual example of gene regulatory diversity, the R-sc (Sc, strong seed color) and the R-p (P, plant color) alleles of r have nonoverlapping tissue specificity and nonhomologous 5' flank...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::A genetic model with additive-dominance effects and genotype x environment interactions is presented for quantitative traits with time-dependent measures. The genetic model for phenotypic means at time t conditional on phenotypic means measured at previous time (t-1) is defined. Statistical methods are proposed for an...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::The N-terminal tail of Ndc80 is essential for kinetochore-microtubule binding in human cells but is not required for viability in yeast. We show that the yeast Ndc80 tail is required for timely mitotic progression and accurate chromosome segregation. The tail is essential when cells are limited for Dam1, demonstrating...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.143818
更新日期:2012-10-01 00:00:00
abstract::The relationships between synonymous and nonsynonymous substitution rates and between synonymous rate and codon usage bias are important to our understanding of the roles of mutation and selection in the evolution of Drosophila genes. Previous studies used approximate estimation methods that ignore codon bias. In this...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Evolutionary models appropriate for analyzing nucleotide sequences that are subject to constraints on secondary structure are developed. The models consider the evolution of pairs of nucleotides, and they incorporate the effects of base-pairing constraints on nucleotide substitution rates by introducing a new paramete...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::Since the publication of the first comprehensive linkage map for the laboratory mouse, the architecture of recombination as a basic biological process has become amenable to investigation in mammalian model organisms. Here we take advantage of high-density genotyping and the unique pedigree structure of the incipient ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.161653
更新日期:2014-05-01 00:00:00
abstract::The larval body wall muscles of Drosophila melanogaster arise by fusion of founder myoblasts (FMs) and fusion-competent myoblasts (FCMs). Sticks-and-Stones (SNS) is expressed on the surface of all FCMs and mediates adhesion with FMs and developing syncytia. Intracellular components essential for myoblast fusion are th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.083808
更新日期:2008-03-01 00:00:00
abstract::Clinal variation is common for enzymes in the glycolytic pathway for Drosophila melanogaster and is generally accepted as an adaptive response to different climates. Although the enzyme phosphoglucomutase (PGM) possesses several allozyme polymorphisms, it is unique in that it had been reported to show no clinal variat...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::Receptor tyrosine kinases (RTKs) are an important family of signaling molecules with the unusual property that they are able to transduce their signals using the same downstream pathways. This has led to an unresolved debate as to whether individual receptors are interchangeable, or if each receptor can mediate specif...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.098475
更新日期:2009-04-01 00:00:00
abstract::Molecular heterogeneity in human breast cancer has challenged diagnosis, prognosis, and clinical treatment. It is well known that molecular subtypes of breast tumors are associated with significant differences in prognosis and survival. Assuming that the differences are attributed to subtype-specific pathways, we then...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.180125
更新日期:2015-12-01 00:00:00
abstract::Genetic analysis of 131 independent transpositions of the tetracycline-resistance element Tn10 from a single site in phage P22 into the histidine operon of Salmonella typhimurium reveals that Tn10 insertions are not randomly distributed along this chromosomal target. The insertions occur in 22 different "clusters"; in...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::X chromosome inactivation (XCI) is an epigenetic process that almost completely inactivates one of two X chromosomes in somatic cells of mammalian females. A few genes are known to escape XCI and the mechanism for this escape remains unclear. Here, using mouse trophoblast stem (TS) cells, we address whether particular...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.162800
更新日期:2014-06-01 00:00:00
abstract::An association was found earlier between the Ep(1) gene locus coding for an endopeptidase and the endosperm color gene Y(1) on chromosome 6 of Zea mays. By employing primary trisomics we have unequivocally placed the Ep(1) gene on chromosome 6, closely linked to the Y(1) locus. Additionally we describe new alleles at ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-08-01 00:00:00
abstract::The murine dilute suppressor gene (dsu) suppresses the coat-color phenotype of three pigment mutations, dilute (d), ashen (ash) and leaden (ln), that each produce adendritic melanocytes. Suppression is due to the ability of dsu to partially restore (ash and ln), or almost completely restore (d), normal melanocyte morp...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::A maximum-likelihood method to estimate the recombination fraction and its sampling variance using informative and noninformative half-sib offspring is derived. Estimates of the recombination fraction are biased up to 20 cM when noninformative offspring are discarded. In certain scenarios, the sampling variance can be...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::In Drosophila and other insects egg production is related to the nutrients available. Somehow the nutritional status of the environment is translated into hormonal signs that can be "read" by each individual egg chamber, influencing the decision to either develop into an egg or die. We have shown that BR-C is a contro...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.103.024323
更新日期:2004-08-01 00:00:00
abstract::Knowing the amount of DNA polymorphism is essential to understand the mechanism of maintaining DNA polymorphism in a natural population. The amount of DNA polymorphism can be measured by the average number of nucleotide differences per site (pi), the proportion of segregating (polymorphic) site (s) and the minimum num...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::Stabilizing selection for an intermediate optimum is generally considered to deplete genetic variation in quantitative traits. However, conflicting results from various types of models have been obtained. While classical analyses assuming a large number of independent additive loci with individually small effects indi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::The progeny of crosses between wild-type strains of Phycomyces usually do not exhibit all of the expected genotypes from meiosis. By backcrossing, we have isolated a new (+) mating-type strain, A56, which is nearly isogenic with the (-) wild-type NRRL1555 commonly used in Phycomyces research. Tetrad analysis of the ba...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-12-01 00:00:00
abstract::Dis3 encodes a conserved RNase that degrades or processes all RNA species via an N-terminal PilT N terminus (PIN) domain and C-terminal RNB domain that harbor, respectively, endonuclease activity and 3'-5' exonuclease activity. In Schizosaccharomyces pombe, dis3 mutations cause chromosome missegregation and failure in...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.187930
更新日期:2016-06-01 00:00:00
abstract::Interchromosomal duplications are especially important for the study of X-linked genes. Males inheriting a mutation in a vital X-linked gene cannot survive unless there is a wild-type copy of the gene duplicated elsewhere in the genome. Rescuing the lethality of an X-linked mutation with a duplication allows the mutat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.123265
更新日期:2010-12-01 00:00:00
abstract::A recent sperm-typing study by Jeffreys and Neumann suggested that recombination rates in different individuals at the DNA2 recombination hotspot appeared to be highly dependent on their genotype at a particular A/G SNP, FG11. Specifically, individuals who carried at least one copy of the A allele at this SNP exhibite...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.039271
更新日期:2006-03-01 00:00:00
abstract::Reciprocal recurrent selection (RRS), which assumes overdominant loci to be important, alters two genetically different populations to improve their crossbred mean. Individual plants from two populations (A and B) are selfed and also crossed with plants from the reciprocal female tester population (B and A, respective...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00
abstract::RNA editing refers to post-transcriptional processes that alter the base sequence of RNA. Recently, hundreds of new RNA editing targets have been reported. However, the mechanisms that determine the specificity and degree of editing are not well understood. We examined quantitative variation of site-specific editing i...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.179481
更新日期:2016-02-01 00:00:00
abstract::The knotted1 gene was first defined by dominant mutations that affect leaf morphology. The original allele, Kn1-O, results from a 17-kb tandem duplication. Mutator (Mu) insertions near the junction of the two repeats suppress the leaf phenotype to different degrees depending on the position of the insertion. The Mu in...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::Previous association analyses showed that variation at major regulatory genes contributes to standing variation for complex traits in Balsas teosinte, the progenitor of maize. This study expands our previous association mapping effort in teosinte by testing 123 markers in 52 candidate genes for association with 31 tra...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.090134
更新日期:2008-10-01 00:00:00
abstract::In many organisms, female and male meiosis display extensive sexual dimorphism in the temporal meiotic program, the number and location of recombination events, sex chromosome segregation, and checkpoint function. We show here that both meiotic prophase timing and germ-line apoptosis, one output of checkpoint signalin...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.110338
更新日期:2010-03-01 00:00:00
abstract::Chromatin insulators separate active from repressed chromatin domains. In yeast the RNA pol III transcription machinery bound to tRNA genes function with histone acetylases and chromatin remodelers to restrict the spread of heterochromatin. Our results collectively demonstrate that binding of TFIIIC is necessary for i...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.106203
更新日期:2009-09-01 00:00:00
abstract::Heparan sulfates (HS) are linear polysaccharides with complex modification patterns, which are covalently bound via conserved attachment sites to core proteins to form heparan sulfate proteoglycans (HSPGs). HSPGs regulate many aspects of the development and function of the nervous system, including cell migration, mor...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.198739
更新日期:2017-08-01 00:00:00