Abstract：
:Induction of expression of immediate-early gene c-Fos in the striatum is a common effect of many drugs of abuse, including morphine. Previous studies have shown that the morphine-mediated c-Fos response is attenuated by antagonists of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor. Other evidence suggests that the NDMA receptor may be coupled to the enzyme neuronal nitric oxide synthase (nNOS). NMDA receptor-mediated increases in intracellular calcium can activate nNOS, which catalyzes the formation of the signaling molecule nitric oxide. Because activation of NMDA receptors mediates morphine-induced c-Fos expression, we tested the hypothesis that activation of nNOS is involved in this cascade. Male rats were injected with the nNOS-selective inhibitor 7-nitroindazole (7-NI) or vehicle 30 min prior to injection of morphine sulfate or vehicle. Two hours later they were perfused with fixative and the brains removed for immunocytochemical analysis for c-Fos. Morphine induced c-Fos expression in the striatum, cerebral cortex, and midline/intralaminar nuclei of thalamus. Expression in the striatum, but not thalamus or cortex, was significantly blocked by 7-NI. Double-label immunocytochemistry revealed no co-localization of c-Fos and nNOS in any brain region. These results support a role for nNOS in the neural circuits activated by morphine.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Harlan RE,Webber DS,Garcia MMdoi
10.1016/s0361-9230(00)00451-2keywords:
subject
Has Abstractpub_date
2001-01-15 00:00:00pages
207-12issue
2eissn
0361-9230issn
1873-2747pii
S0361-9230(00)00451-2journal_volume
54pub_type
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Brain research bulletin
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journal_title：Brain research bulletin
pub_type： 杂志文章
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abstract：BACKGROUND:Traditional Medicine Practices (TMP) which are premised on indigenous knowledge and experiences within a local context of the culture and environment, are common place in low income countries. In Africa and in Uganda specifically, nearly 80% of the Ugandan population relies on TMP for the care of their menta...
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pub_type： 杂志文章,评审
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