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Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein.

Abstract：

:A maltodextrin-binding protein from Pyrococcus furiosus (PfuMBP) has been overproduced in Escherichia coli, purified, and crystallized. The crystal structure of the protein bound to an oligosaccharide ligand was determined to 1.85 A resolution. The fold of PfuMBP is very similar to that of the orthologous MBP from E. coli (EcoMBP), despite the moderate level of sequence identity between the two proteins (27 % identity, 46 % similarity). PfuMBP is extremely resistant to heat and chemical denaturation, which may be attributed to a number of factors, such as a tightly packed hydrophobic core, clusters of isoleucine residues, salt-bridges, and the presence of proline residues in key positions. Surprisingly, an attempt to crystallize the complex of PfuMBP with maltose resulted in a structure that contained maltotriose in the ligand-binding site. The structure of the complex suggests that there is a considerable energy gain upon binding of maltotriose in comparison to maltose. Moreover, isothermal titration calorimetry experiments demonstrated that the binding of maltotriose to the protein is exothermic and tight, whereas no thermal effect was observed upon addition of maltose at three temperatures. Therefore, PfuMBP evidently is designed to bind oligosaccharides composed of three or more glucopyranose units.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Evdokimov AG,Anderson DE,Routzahn KM,Waugh DS

doi

10.1006/jmbi.2000.4202

keywords:

subject

Has Abstract

pub_date

2001-01-26 00:00:00

pages

891-904

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(00)94202-5

journal_volume

305

pub_type

杂志文章

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