Contribution of the hydrophobic effect to globular protein stability.

abstract：:Nicotine is the principal addictive component of tobacco. Blocking its passage from the lung to the brain with nicotine-specific antibodies is a promising approach for the treatment of smoking addiction. We have determined the crystal structure of nicotine bound to the Fab fragment of a fully human monoclonal antibody...

journal_title：Journal of molecular biology

authors： Tars K,Kotelovica S,Lipowsky G,Bauer M,Beerli RR,Bachmann MF,Maurer P

更新日期：2012-01-06 00:00:00

abstract：:Ferrochelatase (protoheme ferrolyase, EC 4.99.1.1) is the terminal enzyme in heme biosynthesis and catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme IX (heme). Due to the many critical roles of heme, synthesis of heme is required by the vast majority of organisms. Despite significant inv...

journal_title：Journal of molecular biology

authors： Medlock AE,Dailey TA,Ross TA,Dailey HA,Lanzilotta WN

更新日期：2007-11-02 00:00:00

abstract：:Gap junction channels are intercellular channels that form by docking the extracellular loops of connexin protein subunits. While the structure and function of gap junctions as intercellular channels have been characterized using different techniques, the physics of the inter-connexin interaction remain unknown. Moreo...

journal_title：Journal of molecular biology

authors： Rico F,Oshima A,Hinterdorfer P,Fujiyoshi Y,Scheuring S

更新日期：2011-09-09 00:00:00

abstract：:The N-terminal segment (FR-H1) of the heavy chain (V(H)) of antibodies shows significant conformational variability correlating with the nature of the amino acids H6, H7 and H10 (Kabat H9). In this study, we have established a causal relationship between the local sequence and the structure of this framework region an...

journal_title：Journal of molecular biology

authors： Jung S,Spinelli S,Schimmele B,Honegger A,Pugliese L,Cambillau C,Plückthun A

更新日期：2001-06-08 00:00:00

abstract：:The 14-3-3 family of proteins plays a role in a wide variety of cellular functions including regulation of protein kinase C and exocytosis. Using antisera specific for the N termini of 14-3-3 isoforms described previously and an additional antiserum specific for the C terminus of epsilon isoform, protease digestion of...

journal_title：Journal of molecular biology

authors： Jones DH,Martin H,Madrazo J,Robinson KA,Nielsen P,Roseboom PH,Patel Y,Howell SA,Aitken A

更新日期：1995-01-27 00:00:00

abstract：:Yeast proteinase B inhibitor 2 (YIB2), which is composed of 74 amino acid residues, is an unusual serine protease inhibitor, since it lacks disulfide bonds. To identify its reactive site for proteases, we constructed an expression system for a synthetic YIB2 gene and then attempted to change the inhibitory properties ...

journal_title：Journal of molecular biology

authors： Kojima S,Deguchi M,Miura K

更新日期：1999-02-26 00:00:00

abstract：:Human pancreatic alpha-amylase has been isolated using a glycogen affinity precipitation procedure and crystallized in a form suitable for high resolution three-dimensional X-ray crystallographic analyses. Crystals are of the orthorhombic space group P2(1)2(1)2(1), with unit cell dimensions of a = 53.04 A, b = 74.80 A...

journal_title：Journal of molecular biology

authors： Burk D,Wang Y,Dombroski D,Berghuis AM,Evans SV,Luo Y,Withers SG,Brayer GD

更新日期：1993-04-05 00:00:00

abstract：:Riboflavin is an essential cofactor in all organisms. Its direct biosynthetic precursor, 6,7-dimethyl-8-ribityllumazine, is synthesised by the enzyme 6,7-dimethyl-8-ribityllumazine synthase. Recently, we have found that the enzyme from Schizosaccharomyces pombe binds riboflavin, the final product of the pathway with a...

journal_title：Journal of molecular biology

authors： Gerhardt S,Haase I,Steinbacher S,Kaiser JT,Cushman M,Bacher A,Huber R,Fischer M

更新日期：2002-05-17 00:00:00

abstract：:In response to intracellular stress events ranging from starvation to pathogen invasion, the cell activates one or more forms of macroautophagy. The key event in these related pathways is the de novo formation of a new organelle called the autophagosome, which either surrounds and sequesters random portions of the cyt...

journal_title：Journal of molecular biology

authors： Nguyen N,Shteyn V,Melia TJ

更新日期：2017-02-17 00:00:00

abstract：:Motifs N2 and N3, also referred to as switch-1 and switch-2, form part of the active site of molecular motors such as myosins and kinesins. In the case of myosin, N3 is thought to act as a gamma-phosphate sensor and moves almost 6 A relative to N2 during the catalysed turnover of ATP, opening and closing the active si...

journal_title：Journal of molecular biology

authors： Furch M,Fujita-Becker S,Geeves MA,Holmes KC,Manstein DJ

更新日期：1999-07-16 00:00:00

abstract：:C-tailed membrane proteins insert into the bilayer post-translationally because the hydrophobic anchor segment leaves the ribosome at the end of translation. Nevertheless, we find here evidence that the targeting of SciP to the membrane of Escherichia coli occurs co-translationally since signal elements in the N-termi...

journal_title：Journal of molecular biology

authors： Pross E,Soussoula L,Seitl I,Lupo D,Kuhn A

更新日期：2016-10-09 00:00:00

abstract：:Protein-protein interactions within the membrane, partially or fully mediated by transmembrane (TM) domains, are involved in many vital cellular processes. Since the unique feature of the membrane environment enables protein-protein assembly that otherwise is not energetically favored in solution, the structural restr...

journal_title：Journal of molecular biology

authors： Sal-Man N,Gerber D,Shai Y

更新日期：2004-11-26 00:00:00

abstract：:The morphogene bolA is a general stress response gene in Escherichia coli that induces a round morphology when overexpressed. Results presented in this report show that increased BolA levels can inhibit cell elongation mechanisms. MreB polymerization is crucial for the bacterial cell cytoskeleton, and this protein is ...

journal_title：Journal of molecular biology

authors： Freire P,Moreira RN,Arraiano CM

更新日期：2009-02-06 00:00:00

abstract：:The catalytic activity and substrate channeling of the pyridoxal 5'-phosphate-dependent tryptophan synthase alpha(2)beta(2) complex is regulated by allosteric interactions that modulate the switching of the enzyme between open, low activity and closed, high activity states during the catalytic cycle. The highly conser...

journal_title：Journal of molecular biology

authors： Kulik V,Weyand M,Seidel R,Niks D,Arac D,Dunn MF,Schlichting I

更新日期：2002-12-06 00:00:00

abstract：:Aquaporin-1 (AQP1) is an abundant protein in human erythrocyte membranes which functions as a specific and constitutively active water conducting pore. Solubilized and isolated as tetramer, it forms well-ordered two-dimensional (2D) crystals when reconstituted in the presence of lipids. Several high resolution project...

journal_title：Journal of molecular biology

authors： Walz T,Tittmann P,Fuchs KH,Müller DJ,Smith BL,Agre P,Gross H,Engel A

更新日期：1996-12-20 00:00:00

abstract：:alpha 1-Antitrypsin is the archetypal member of the serine proteinase inhibitor or serpin superfamily. Members of the family show structural homology based on a dominant A beta-sheet and a mobile reactive centre loop. Our recent crystal structure of alpha 1-antitrypsin stabilized with a point mutation showed the loop ...

journal_title：Journal of molecular biology

authors： Elliott PR,Abrahams JP,Lomas DA

更新日期：1998-01-23 00:00:00

abstract：:The scrapie prion protein (PrPSc) is formed from the cellular isoform (PrPC) by a post-translational process that involves a profound conformational change. Linear epitopes for recombinant antibody Fab fragments (Fabs) on PrPC and on the protease-resistant core of PrPSc, designated PrP 27-30, were identified using ELI...

journal_title：Journal of molecular biology

authors： Peretz D,Williamson RA,Matsunaga Y,Serban H,Pinilla C,Bastidas RB,Rozenshteyn R,James TL,Houghten RA,Cohen FE,Prusiner SB,Burton DR

更新日期：1997-10-31 00:00:00

abstract：:Although largely deemed as structurally conserved, catalytic metal ion sites can rearrange, thereby contributing to enzyme evolvability. Here, we show that in paraoxonase-1, a lipo-lactonase, catalytic promiscuity and divergence into an organophosphate hydrolase are correlated with an alternative mode of the catalytic...

journal_title：Journal of molecular biology

authors： Ben-David M,Wieczorek G,Elias M,Silman I,Sussman JL,Tawfik DS

更新日期：2013-03-25 00:00:00

abstract：:Low molecular weight protein tyrosine phosphatase (LMW-PTP) dimerizes in the phosphate-bound state in solution with a dissociation constant of K(d)=1.5(+/-0.1)mM and an off-rate on the order of 10(4)s(-1). 1H and 15N NMR chemical shifts identify the dimer interface, which is in excellent agreement with that observed i...

journal_title：Journal of molecular biology

authors： Akerud T,Thulin E,Van Etten RL,Akke M

更新日期：2002-09-06 00:00:00

abstract：:Tissue deposition of normally soluble proteins as insoluble amyloid fibrils is associated with serious diseases including the systemic amyloidoses, maturity onset diabetes, Alzheimer's disease and transmissible spongiform encephalopathy. Although the precursor proteins in different diseases do not share sequence homol...

journal_title：Journal of molecular biology

authors： Sunde M,Serpell LC,Bartlam M,Fraser PE,Pepys MB,Blake CC

更新日期：1997-10-31 00:00:00

abstract：:Bovine IF(1) is a basic, 84 amino acid residue protein that inhibits the hydrolytic action of the F(1)F(0) ATP synthase in mitochondria under anaerobic conditions. Its oligomerization state is dependent on pH. At a pH value below 6.5 it forms an active dimer. At higher pH values, two dimers associate to form an inacti...

journal_title：Journal of molecular biology

authors： Gordon-Smith DJ,Carbajo RJ,Yang JC,Videler H,Runswick MJ,Walker JE,Neuhaus D

更新日期：2001-04-27 00:00:00

abstract：:The role of the sigma 70 subunit of Escherichia coli RNA polymerase in transcription activation by positive transcription factors was investigated. For this purpose, we constructed a nested set of E. coli rpoD deletion mutants generating carboxy-terminally truncated sigma 70 subunits of RNA polymerase in a high-expres...

journal_title：Journal of molecular biology

authors： Kumar A,Grimes B,Fujita N,Makino K,Malloch RA,Hayward RS,Ishihama A

更新日期：1994-01-14 00:00:00

abstract：:The X-ray crystal structure of the sulfide derivative of ferric Lucina pectinata hemoglobin component I (HbI) has been determined at 1.9 A resolution (R-factor 0.186). The heme pocket structural organization of HbI is in keeping with its ligand binding properties. The fast sulfide association rate constant can be rela...

journal_title：Journal of molecular biology

authors： Rizzi M,Wittenberg JB,Coda A,Ascenzi P,Bolognesi M

更新日期：1996-04-26 00:00:00

abstract：:Binding of the histone (H2A, H2B) dimer with chicken erythrocyte DNA has been studied by salt-titration spectroscopy in equilibrium conditions. The circular dichroism of DNA near 275 nm is depressed by the interaction with (H2A, H2B) at low concentrations of salt. The depression increases with increasing amounts of (H...

journal_title：Journal of molecular biology

authors： Oohara I,Wada A

更新日期：1987-07-20 00:00:00

abstract：:We have applied the method of simulated annealing to the refinement of the 3 A resolution crystal structure of the influenza virus hemagglutinin glycoprotein, using the program X-PLOR. Two different methods were introduced into X-PLOR to treat the non-crystallographic symmetry present in this and in other crystal stru...

journal_title：Journal of molecular biology

authors： Weis WI,Brünger AT,Skehel JJ,Wiley DC

更新日期：1990-04-20 00:00:00

abstract：:The single mutation L30 K in the Hu-Yap65 WW domain increased the stability of the complex with the peptide GTPPPPYTVG (K(d)=40(+/-5) microM). Here we report the refined solution structure of this complex by NMR spectroscopy and further derived structure-activity relationships by using ligand peptide libraries with tr...

journal_title：Journal of molecular biology

authors： Pires JR,Taha-Nejad F,Toepert F,Ast T,Hoffmüller U,Schneider-Mergener J,Kühne R,Macias MJ,Oschkinat H

更新日期：2001-12-14 00:00:00

abstract：:The cis/trans isomerization of prolyl peptide bonds has been suggested to dominate the folding of the alpha subunit of tryptophan synthase from Escherichia coli (alphaTS). To test the role of the unique cis isomer between Asp27 and Pro28, the folding properties of P28A, P28G and G(3)P28G, a three-glycine insertion mut...

journal_title：Journal of molecular biology

authors： Wu Y,Matthews CR

更新日期：2002-09-06 00:00:00

abstract：:The FLP recombinase interacts with its target sequence with the formation of three distinct DNA-protein complexes. The first complex leaves neither a DNase footprint nor is the DNA protected from methylation by dimethyl sulfate. We have found, however, that the FLP protein is bound predominantly to only one of the thr...

journal_title：Journal of molecular biology

authors： Beatty LG,Sadowski PD

更新日期：1988-11-20 00:00:00

abstract：:Riboswitches are complex folded RNA domains found in noncoding regions of mRNA that regulate gene expression upon small molecule binding. Recently, Breaker and coworkers reported a tandem aptamer riboswitch (VCI-II) that binds glycine cooperatively. Here, we use hydroxyl radical footprinting and small-angle X-ray scat...

journal_title：Journal of molecular biology

authors： Lipfert J,Das R,Chu VB,Kudaravalli M,Boyd N,Herschlag D,Doniach S

更新日期：2007-02-02 00:00:00

abstract：:Automated protein redesign, as implemented in the program ORBIT, was used to redesign the core of phage T4 lysozyme. A total of 26 buried or partially buried sites in the C-terminal domain were allowed to vary both their sequence and side-chain conformation while the backbone and non-selected side-chains remained fixe...

journal_title：Journal of molecular biology

authors： Mooers BH,Datta D,Baase WA,Zollars ES,Mayo SL,Matthews BW

更新日期：2003-09-19 00:00:00