Common core structure of amyloid fibrils by synchrotron X-ray diffraction.

abstract：:We have investigated the translational and rotational diffusion of two proteins, bovine pancreatic trypsin inhibitor and hen egg white lysozyme, using molecular dynamics simulations in explicit solvent. The translational diffusion constants obtained from the simulations compare favourably with results from experiments...

journal_title：Journal of molecular biology

authors： Smith PE,van Gunsteren WF

更新日期：1994-02-18 00:00:00

abstract：:The Cip/Kip family of cyclin-dependent kinase (Cdk) inhibitors includes p21(Cip1), p27(Kip1) and p57(Kip2). Their kinase inhibitory activities are mediated by a homologous N-terminal kinase inhibitory domain. The Cdk inhibitory activity and stability of p27 have been shown to be regulated by a two-step phosphorylation...

journal_title：Journal of molecular biology

authors： Huang Y,Yoon MK,Otieno S,Lelli M,Kriwacki RW

更新日期：2015-01-30 00:00:00

abstract：:Protein self-assembly is a common and essential biological phenomenon, and bacterial microcompartments present a promising model system to study this process. Bacterial microcompartments are large, protein-based organelles which natively carry out processes important for carbon fixation in cyanobacteria and the surviv...

journal_title：Journal of molecular biology

authors： Kennedy NW,Ikonomova SP,Slininger Lee M,Raeder HW,Tullman-Ercek D

更新日期：2021-01-22 00:00:00

abstract：:Only a few reports have been published on the interactions between Coxsackievirus B4 (CVB4) and human peripheral blood mononuclear cells (PBMC) but have not been extensively documented. Human serum containing non-neutralizing anti-CVB4 antibodies increased CVB4-induced synthesis of IFNα by PBMC. In this study, we dete...

journal_title：Journal of molecular biology

authors： Alidjinou EK,Sané F,Engelmann I,Hober D

更新日期：2013-12-13 00:00:00

abstract：:Dystrophin Dp71 is the main product of the Duchenne muscular dystrophy gene in the brain; however, its function is unknown. To study the role of Dp71 in neuronal cells, we previously generated by antisense treatment PC12 neuronal cell clones with decreased Dp71 expression (antisense-Dp71 cells). PC12 cells express two...

journal_title：Journal of molecular biology

authors： Cerna J,Cerecedo D,Ortega A,García-Sierra F,Centeno F,Garrido E,Mornet D,Cisneros B

更新日期：2006-10-06 00:00:00

abstract：:Sequence-specific interactions between proteins and DNA are essential for a variety of biological functions. The (cytosine-C5)-methyltransferase from HhaI (M.HhaI) specifically modifies the second base in GCGC sequences, employing a base flipping mechanism to access the target base being chemically modified. The mecha...

journal_title：Journal of molecular biology

authors： Huang N,MacKerell AD Jr

更新日期：2005-01-14 00:00:00

abstract：:Transcription by RNA polymerase II (Pol II) is required to produce mRNAs and some noncoding RNAs (ncRNAs) within mammalian cells. This coordinated process is precisely regulated by multiple factors, including many recently discovered ncRNAs. In this perspective, we will discuss newly identified ncRNAs that facilitate ...

journal_title：Journal of molecular biology

authors： Eidem TM,Kugel JF,Goodrich JA

更新日期：2016-06-19 00:00:00

abstract：:The coat protein of positive-stranded RNA viruses often contains a positively charged tail that extends toward the center of the capsid and interacts with the viral genome. Electrostatic interaction between the tail and the RNA has been postulated as a major force in virus assembly and stabilization. The goal of this ...

journal_title：Journal of molecular biology

authors： Ni P,Wang Z,Ma X,Das NC,Sokol P,Chiu W,Dragnea B,Hagan M,Kao CC

更新日期：2012-06-22 00:00:00

abstract：:The function of GroE requires a complex system of allosteric communication driven by protein-nucleotide interactions. These rearrangements couple the binding and hydrolysis of ATP to an overall reaction cycle in which substrate proteins are bound, encapsulated and released. Positive cooperativity with respect to ATP b...

journal_title：Journal of molecular biology

authors： Poso D,Clarke AR,Burston SG

更新日期：2004-05-14 00:00:00

abstract：:Proteins of the Mob1/phocein family are found in all eukaryotic cells. In yeast, they are activating subunits of Dbf2-related protein kinases involved in cell cycle control. Despite the wide occurrence of these proteins, their biological functions remain poorly understood. Here we report the solution structure of the ...

journal_title：Journal of molecular biology

authors： Ponchon L,Dumas C,Kajava AV,Fesquet D,Padilla A

更新日期：2004-03-12 00:00:00

abstract：:Collagen fibrils are the principle source of mechanical strength in the mutable dermis of the sea cucumber Cucumaria frondosa. To obtain information about the mechanism by which collagen molecules self-assemble into fibrils, we have isolated single intact fibrils with lengths in the range 14-444 microm. These fibrils ...

journal_title：Journal of molecular biology

authors： Trotter JA,Chapman JA,Kadler KE,Holmes DF

更新日期：1998-12-18 00:00:00

abstract：:The glycolytic enzyme phosphoglycerate kinase (PGK) catalyzes phosphoryl transfer between 1,3-bis-phosphoglycerate and ADP to form 3-phosphoglycerate and ATP. During catalysis, a major hinge bending motion occurs which brings the N and C-terminal enzyme domains and their bound substrates together and in-line for phosp...

journal_title：Journal of molecular biology

authors： Bernstein BE,Williams DM,Bressi JC,Kuhn P,Gelb MH,Blackburn GM,Hol WG

更新日期：1998-06-26 00:00:00

abstract：:The structures of three crystal forms of the class mu human glutathione transferase GSTM2-2 have been determined. X-ray phase information was obtained independently from molecular replacement and from anomalous scattering by a single isomorphous derivative. One crystal form contains a single monomer in the asymmetric ...

journal_title：Journal of molecular biology

authors： Raghunathan S,Chandross RJ,Kretsinger RH,Allison TJ,Penington CJ,Rule GS

更新日期：1994-05-20 00:00:00

abstract：:The vitamin D-binding protein, Gc, was purified from human serum and crystallized using the hanging-drop method. The best crystals were grown from 28% polyethylene glycol 400 in 50 mM-sodium acetate at pH 4.8. These crystals diffract to 3.4 A and the observed diffraction is consistent with orthorhombic space groups P4...

journal_title：Journal of molecular biology

authors： Vogelaar NJ,Lindberg U,Schutt CE

更新日期：1991-08-05 00:00:00

abstract：:When influenza A virus infects host cells, its integral matrix protein M2 forms a proton-selective channel in the viral envelope. Although X-ray crystallography and NMR studies using fragment peptides have suggested that M2 stably forms a tetrameric channel irrespective of pH, the oligomeric states of the full-length ...

journal_title：Journal of molecular biology

authors： Kawano K,Yano Y,Matsuzaki K

更新日期：2014-07-15 00:00:00

abstract：:The bacteriophage T3 and T7 RNA polymerases (RNAP) are closely related, yet exhibit high specificity for their own promoter sequences. In this work the primary determinant of T7 versus T3 promoter specificity has been localized to a single amino acid residue at position 748 in the T7 RNAP. Substitution of this residue...

journal_title：Journal of molecular biology

authors： Raskin CA,Diaz G,Joho K,McAllister WT

更新日期：1992-11-20 00:00:00

abstract：:Proteasomes, major proteolytic sites in eukaryotic cells, play an important part in major histocompatibility class I (MHC I) ligand generation and thus in the regulation of specific immune responses. Their cleavage specificity is of outstanding interest for this process. In order to generalize previously determined cl...

journal_title：Journal of molecular biology

authors： Kuttler C,Nussbaum AK,Dick TP,Rammensee HG,Schild H,Hadeler KP

更新日期：2000-05-05 00:00:00

abstract：:Iron loss in vitro by the iron scavenger bovine lactoferrin was investigated in acidic media in the presence of three different monoanions (NO(3)(-), Cl(-) and Br(-)) and one dianion (SO(4)(2-)). Holo and monoferric C-site lactoferrins lose iron in acidic media (pH< or =3.5) by a four-step mechanism. The first two ste...

journal_title：Journal of molecular biology

authors： Abdallah FB,El Hage Chahine JM

更新日期：2000-10-20 00:00:00

abstract：:The pfkB gene of Escherichia coli is known to specify a minor phosphofructokinase, Pfk-2, in the wild-type strain; the pfkB1 mutation causes a 25-fold increase in the amount of Pfk-2 so that it adequately substitutes for mutational loss of the major phosphofructokinase, Pfk-1 (specified by pfkA); and another closely l...

journal_title：Journal of molecular biology

authors： Daldal F

更新日期：1983-08-05 00:00:00

abstract：:Transcription of the Bacillus subtilis iol divergon is negatively regulated by a repressor encoded by iolR, which belongs to the DeoR family of bacterial regulators. Gel retardation analysis involving the IolR protein synthesized in Escherichia coli revealed that IolR bound specifically and independently to each of th...

journal_title：Journal of molecular biology

authors： Yoshida KI,Shibayama T,Aoyama D,Fujita Y

更新日期：1999-01-22 00:00:00

abstract：:Residue-specific potentials between pairs of side-chains and pairs of side-chain-backbone interaction sites have been generated by collecting radial distribution data for 302 protein structures. Multiple atomic interactions have been utilized to enhance the specificity and smooth the distance-dependence of the potenti...

journal_title：Journal of molecular biology

authors： Bahar I,Jernigan RL

更新日期：1997-02-14 00:00:00

abstract：:The precision of the repair of linearized plasmid DNA was analyzed using a nonsense mutation inactivated beta-glucuronidase (uidA) marker gene delivered to Nicotiana plumbaginifolia protoplasts and Nicotiana tabacum leaves. The reversions at the stop-codon allowed the reactivation of the marker gene. Here we report th...

journal_title：Journal of molecular biology

authors： Ilnytskyy Y,Yao Y,Kovalchuk I

更新日期：2005-01-28 00:00:00

abstract：:Membranes have a potent ability to promote secondary structure formation in a wide range of membrane-active peptides, believed to be due to a reduction through hydrogen bonding of the energetic cost of partitioning peptide bonds. This process is of fundamental importance for understanding the mechanism of action of to...

journal_title：Journal of molecular biology

authors： Ladokhin AS,White SH

更新日期：1999-01-29 00:00:00

abstract：:The structure of cardiotoxin CTX I from Naja naja atra has been investigated by NMR spectroscopy. Sequence specific resonance assignments have been obtained for all backbone protons as well as for most side-chain protons. Distance geometry calculations were carried out using a metric matrix DG program. A total of 715 ...

journal_title：Journal of molecular biology

authors： Jahnke W,Mierke DF,Béress L,Kessler H

更新日期：1994-07-29 00:00:00

abstract：:A Rhizobium meliloti DNA region (nodD1) involved in the regulation of other early nodulation genes has been delimited by directed Tn5 mutagenesis and its nucleotide sequence has been determined. The sequence data indicate a large open reading frame with opposite polarity to nodA, -B and -C, coding for a protein of 308...

journal_title：Journal of molecular biology

authors： Göttfert M,Horvath B,Kondorosi E,Putnoky P,Rodriguez-Quiñones F,Kondorosi A

更新日期：1986-10-05 00:00:00

abstract：:The isolated H(+)-ATPase from Escherichia coli (EF(0)F(1)) was investigated by electron microscopy of samples of negatively stained monodisperse molecules, followed by single-particle image processing. The resulting three-dimensional maps showed that the F(1)-part is connected by a prominent stalk to a more peripheral...

journal_title：Journal of molecular biology

authors： Böttcher B,Bertsche I,Reuter R,Gräber P

更新日期：2000-02-18 00:00:00

abstract：:Antimycin A (antimycin), one of the first known and most potent inhibitors of the mitochondrial respiratory chain, binds to the quinone reduction site of the cytochrome bc1 complex. Structure-activity relationship studies have shown that the N-formylamino-salicyl-amide group is responsible for most of the binding spec...

journal_title：Journal of molecular biology

authors： Huang LS,Cobessi D,Tung EY,Berry EA

更新日期：2005-08-19 00:00:00

abstract：:The resonances of all the base protons and most of the sugar protons in both strands of the 17 base-pair OR3 operator of the phage lambda, and of the vC3 single base-pair mutant, have been assigned using two-dimensional nuclear magnetic resonance methods. The chemical shift and nuclear Overhauser effect data for these...

journal_title：Journal of molecular biology

authors： Wemmer DE,Chou SH,Reid BR

更新日期：1984-11-25 00:00:00

abstract：:Crystallization of membrane proteins is a major stumbling block en route to elucidating their structure and understanding their function. The novel concept of membrane protein crystallization from lipidic cubic phases, "in cubo", has yielded well-ordered crystals and high-resolution structures of several membrane prot...

journal_title：Journal of molecular biology

authors： Qutub Y,Reviakine I,Maxwell C,Navarro J,Landau EM,Vekilov PG

更新日期：2004-11-05 00:00:00

abstract：:Two-dimensional (2D) agarose gel electrophoresis is progressively replacing electron microscopy as the technique of choice to map the initiation and termination sites for DNA replication. Two different versions were originally developed to analyze the replication of the yeast 2 microns plasmid. Neutral/Neutral (N/N) 2...

journal_title：Journal of molecular biology

authors： Martín-Parras L,Hernández P,Martínez-Robles ML,Schvartzman JB

更新日期：1991-08-20 00:00:00