当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme.

Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme.

Abstract：

:The pfkB gene of Escherichia coli is known to specify a minor phosphofructokinase, Pfk-2, in the wild-type strain; the pfkB1 mutation causes a 25-fold increase in the amount of Pfk-2 so that it adequately substitutes for mutational loss of the major phosphofructokinase, Pfk-1 (specified by pfkA); and another closely linked mutation, pfkB10, affects the structure of Pfk-2. This paper is about the pfkB1 mutation. pfkB+, pfkB1 and pfkB1 pfkB10 were cloned and subcloned on plasmid pBR322; their functions were carried, in all three cases, by a 2.1 X 10(3) base-pair fragment with a similar or identical restriction pattern. Experiments with "maxicells" confirmed that the cloned fragments included the structural gene as well as the determinants of its level of expression. Results of N-terminal sequencing of the enzyme matched with the DNA sequence and established the position and direction of the gene. A HindIII-SmaI fragment of 408 base-pairs, which included 294 base-pairs of the non-coding 5' region and 114 base-pairs of the protein coding sequence, was fused to galK on the promoter cloning vector pK01; in the fusion pfkB1 caused a high level expression of galK. Transcription in vitro from pfkB+ and pfkB1 allowed the determination of the +1 position in both cases, at about 19 base-pairs before the initiating methionine codon; the level of transcription was much higher from pfkB1 than from pfkB+. The DNA sequence of the 408 base-pair fragments from pfkB+ and pfkB1 were found to differ only in a single residue, the pfkB1 mutation thus proving to be a C to T change at position about -12 from the initiation of transcription.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Daldal F

doi

10.1016/s0022-2836(83)80019-9

subject

Has Abstract

pub_date

1983-08-05 00:00:00

pages

285-305

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)80019-9

journal_volume

168

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Modular structure in developmentally eliminated DNA in Tetrahymena may be a consequence of frequent insertions and deletions.

    abstract：:The work reported here describes insertion-deletion (Indel) polymorphisms in two internally eliminated sequences (IESs, that are deleted during development in Tetrahymena): a 1.8 kb Indel at one end of the 1.1 kb H1 IES and a 0.5 kb Indel inside the 1.4 kb calmodulin (C) IES. These two IESs are located in the proximit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2003.12.065

    authors： Huvos P

    更新日期：2004-03-05 00:00:00

  • The relationship between conservation, thermodynamic stability, and function in the SH3 domain hydrophobic core.

    abstract：:To investigate the relationships between sequence conservation, protein stability, and protein function, we have measured the thermodynamic stability, folding kinetics, and in vitro peptide-binding activity of a large number of single-site substitutions in the hydrophobic core of the Fyn SH3 domain. Comparison of thes...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2003.08.035

    authors： Di Nardo AA,Larson SM,Davidson AR

    更新日期：2003-10-24 00:00:00

  • Molecular structure of the "low molecular weight antigen" of Toxoplasma gondii: a glucose alpha 1-4 N-acetylgalactosamine makes free glycosyl-phosphatidylinositols highly immunogenic.

    abstract：:Toxoplasma gondii is a ubiquitous parasitic protozoan causing congenital infection and severe encephalitis in the course of the acquired immunodeficiency syndrome. Glycosyl-phosphatidylinositols of T. gondii have been shown to be identical with the low molecular weight antigen which elicits an early immunoglobulin M i...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0806

    authors： Striepen B,Zinecker CF,Damm JB,Melgers PA,Gerwig GJ,Koolen M,Vliegenthart JF,Dubremetz JF,Schwarz RT

    更新日期：1997-03-07 00:00:00

  • Crystallization and preliminary crystallographic study of cytochrome cd1 nitrite reductase from Thiosphaera pantotropha.

    abstract：:Single crystals of cytochrome cd1 nitrite reductase from Thiosphaera pantotropha have been grown in the presence of ammonium sulphate by vapour diffusion techniques. Crystals are suitable for X-ray analysis and diffract to dmin = 1.5 A. They belong to space group P2(1) with unit cell dimensions of a = 107.8 A, b = 61....

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1473

    authors： Fülöp V,Moir JW,Ferguson SJ,Hajdu J

    更新日期：1993-08-20 00:00:00

  • Structural properties of a collagenous heterotrimer that mimics the collagenase cleavage site of collagen type I.

    abstract：:Collagens contain sequence- and conformation-dependent epitopes responsible for their digestion by collagenases at specific loci. A synthetic heterotrimer construct containing the collagenase cleavage site of collagen type I was found to mimic perfectly native collagen in terms of selectivity and mode of enzymatic deg...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00365-0

    authors： Fiori S,Saccà B,Moroder L

    更新日期：2002-06-21 00:00:00

  • Insulin assembly damps conformational fluctuations: Raman analysis of amide I linewidths in native states and fibrils.

    abstract：:The crystal structure of insulin has been investigated in a variety of dimeric and hexameric assemblies. Interest in dynamics has been stimulated by conformational variability among crystal forms and evidence suggesting that the functional monomer undergoes a conformational change on receptor binding. Here, we employ ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00536-9

    authors： Dong J,Wan Z,Popov M,Carey PR,Weiss MA

    更新日期：2003-07-04 00:00:00

  • High-resolution structure of bovine pancreatic trypsin inhibitor with altered binding loop sequence.

    abstract：:A mutant of bovine pancreatic trypsin inhibitor (BPTI) has been constructed and expressed in Escherichia coli in order to probe the kinetic and structural consequences of truncating the binding loop residues to alanine. In addition to two such mutations (Thr11Ala and Pro13Ala), it has a conservative Lys15Arg substitut...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3445

    authors： Czapinska H,Otlewski J,Krzywda S,Sheldrick GM,Jaskólski M

    更新日期：2000-02-04 00:00:00

  • Characterization of two subunits of Arabidopsis 19S proteasome regulatory complex and its possible interaction with the COP9 complex.

    abstract：:The nuclear localized, multi-subunit COP9 complex (or COP9 signalosome) is a key developmental modulator involved in repression of photomorphogenesis. In an effort to further define the molecular actions of the COP9 complex, a yeast two hybrid interactive screen was undertaken to identify proteins that could directly ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2315

    authors： Kwok SF,Staub JM,Deng XW

    更新日期：1999-01-08 00:00:00

  • Rapid folding and unfolding of Apaf-1 CARD.

    abstract：:Caspase recruitment domains (CARDs) are members of the death domain superfamily and contain six antiparallel helices in an alpha-helical Greek key topology. We have examined the equilibrium and kinetic folding of the CARD of Apaf-1 (apoptotic protease activating factor 1), which consists of 97 amino acid residues, at ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.02.105

    authors： Milam SL,Nicely NI,Feeney B,Mattos C,Clark AC

    更新日期：2007-05-25 00:00:00

  • A study of electrophoretic mobility of DNA in agarose and polyacrylamide gels.

    abstract：:The aim of this paper is to clarify the mechanism of gel electrophoresis of DNA under constant-field conditions. We have conducted a large number of experiments on double-stranded DNA varying in length between approximately 10 and approximately 50,000 base-pairs, in both agarose and polyacrylamide gels ranging from 0....

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)80187-y

    authors： Calladine CR,Collis CM,Drew HR,Mott MR

    更新日期：1991-10-05 00:00:00

  • Supercoiling-dependent site-specific binding of HU to naked Mu DNA.

    abstract：:Using HU chemical nucleases to probe HU-DNA interactions, we report here for the first time site-specific binding of HU to naked DNA. An unique feature of this interaction is the absolute requirement for negative DNA supercoiling for detectable levels of site-specific DNA binding. The HU binding site is the Mu spacer ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2805

    authors： Kobryn K,Lavoie BD,Chaconas G

    更新日期：1999-06-18 00:00:00

  • Local interactions dominate folding in a simple protein model.

    abstract：:Recent computational studies of simple models of protein folding have concluded that a pronounced energy minimum (i.e. large gap in energy between low-energy states of the model) is a necessary and sufficient condition to ensure folding of a sequence to its lowest-energy conformation. Here, we show that this conclusio...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0375

    authors： Unger R,Moult J

    更新日期：1996-06-28 00:00:00

  • Structure of membrane-bound annexin A5 trimers: a hybrid cryo-EM - X-ray crystallography study.

    abstract：:Annexins constitute a family of phospholipid- and Ca(2+)-binding proteins involved in a variety of membrane-related processes. The property of several annexins, including annexin A5, to self-organize at the surface of lipid membranes into 2D ordered arrays has been proposed to be functionally relevant in cellular cont...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4183

    authors： Oling F,Santos JS,Govorukhina N,Mazères-Dubut C,Bergsma-Schutter W,Oostergetel G,Keegstra W,Lambert O,Lewit-Bentley A,Brisson A

    更新日期：2000-12-08 00:00:00

  • Correlation between conformational and binding properties of nebulin repeats.

    abstract：:Nebulin, a large protein (600 to 800 kDa) located in the thin filament of striated vertebrate muscle, is assumed to bind and stabilise F-actin. Complete sequence determination of human nebulin has only recently been accomplished showing a uniform modular structure along the whole length of the molecule. Up to 97% of t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0169

    authors： Pfuhl M,Winder SJ,Castiglione Morelli MA,Labeit S,Pastore A

    更新日期：1996-03-29 00:00:00

  • HDEA, a periplasmic protein that supports acid resistance in pathogenic enteric bacteria.

    abstract：:The X-ray crystal structure of the Escherichia coli stress response protein HDEA has been determined at 2.0 A resolution. The single domain alpha-helical protein is found in the periplasmic space, where it supports an acid resistance phenotype essential for infectivity of enteric bacterial pathogens, such as Shigella ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3347

    authors： Gajiwala KS,Burley SK

    更新日期：2000-01-21 00:00:00

  • Evolution of differential substrate specificities in Mu class glutathione transferases probed by DNA shuffling.

    abstract：:A library of variant enzymes was created by combined shuffling of the DNA encoding the human Mu class glutathione transferases GST M1-1 and GST M2-2. The parental GSTs are 84 % sequence identical at the protein level, but their specific activities with the substrates aminochrome and 2-cyano-1,3-dimethyl-1-nitrosoguani...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2607

    authors： Hansson LO,Bolton-Grob R,Massoud T,Mannervik B

    更新日期：1999-03-26 00:00:00

  • Quantitative analysis of DNase I digestion patterns of oligo- and polynucleosomes.

    abstract：:DNase I digestion of unlabelled chromatin has been used to determine the orientation of nucleosomes in the higher-order structure of chromatin. DNA digestion patterns were analysed quantitatively and compared with simulation curves that were generated with the experimentally obtained rate constants for cutting inside ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1777

    authors： Staynov DZ,Proykova YG

    更新日期：1998-05-29 00:00:00

  • A Theoretical Framework for Evolutionary Cell Biology.

    abstract：:One of the last uncharted territories in evolutionary biology concerns the link with cell biology. Because all phenotypes ultimately derive from events at the cellular level, this connection is essential to building a mechanism-based theory of evolution. Given the impressive developments in cell biological methodologi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2020.02.006

    authors： Lynch M,Trickovic B

    更新日期：2020-03-27 00:00:00

  • E. coli transports aggregated proteins to the poles by a specific and energy-dependent process.

    abstract：:Aggregation of proteins due to failure of quality control mechanisms is deleterious to both eukaryotes and prokaryotes. We found that in Escherichia coli, protein aggregates are delivered to the pole and form a large polar aggregate (LPA). The formation of LPAs involves two steps: the formation of multiple small aggre...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.07.009

    authors： Rokney A,Shagan M,Kessel M,Smith Y,Rosenshine I,Oppenheim AB

    更新日期：2009-09-25 00:00:00

  • The crystal structure of the trigonal decamer C-G-A-T-C-G-6meA-T-C-G: a B-DNA helix with 10.6 base-pairs per turn.

    abstract：:The B-DNA decanucleotide C-G-A-T-C-G-6meA-T-C-G has been crystallized under the same conditions used earlier for C-G-A-T-C-G-A-T-C-G, but is found to adopt a new trigonal P3(2)21 packing mode instead of the expected orthorhombic P2(1)2(1)2(1) form. Unit cell dimensions a = b = 33.38 A, c = 98.30 A, gamma = 120 degrees...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1325

    authors： Baikalov I,Grzeskowiak K,Yanagi K,Quintana J,Dickerson RE

    更新日期：1993-06-05 00:00:00

  • Amide proton exchange in human metallothionein-2 measured by nuclear magnetic resonance spectroscopy.

    abstract：:In human metallothionein-2, the exchange rate constants of ten amide protons were found to range from 1.7 x 10(-4) to 1 x 10(-1) min-1 at pH 6.3 and 8 degrees C. Most of these slowly exchanging protons could be associated with hydrogen bonds in secondary structure elements of the alpha-domain. Amide proton exchange ra...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90292-T

    authors： Messerle BA,Bos M,Schäffer A,Vasák M,Kägi JH,Wüthrich K

    更新日期：1990-08-05 00:00:00

  • Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy.

    abstract：:The N-terminal large fragments of staphylococcal nuclease (SNase), SNase110 (1-110 residues), SNase121 (1-121 residues), and SNase135 (1-135 residues), and the fragment mutants G88W110, G88W121, V66W110 and V66W121 were studied by heteronuclear multidimensional NMR spectroscopy. Ensembles of co-existent native-like pa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00660-0

    authors： Feng Y,Liu D,Wang J

    更新日期：2003-07-18 00:00:00

  • Crystal structure of the pertussis toxin-ATP complex: a molecular sensor.

    abstract：:Pertussis toxin is a major virulence factor of Bordetella pertussis, the causative agent of whooping cough. The protein is a hexamer containing a catalytic subunit (S1) that is tightly associated with a pentameric cell-binding component (B-oligomer). In vitro experiments have shown that ATP and a number of detergents ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0277

    authors： Hazes B,Boodhoo A,Cockle SA,Read RJ

    更新日期：1996-05-17 00:00:00

  • Nucleosome core particle stability and conformational change. Effect of temperature, particle and NaCl concentrations, and crosslinking of histone H3 sulfhydryl groups.

    abstract：:We have studied the reversible dissociation of core size DNA from chicken erythrocyte nucleosome core particles in solutions containing 0 X 1 M to 0 X 6 M-NaCl. Dissociation increases with increasing NaCl concentration, increasing temperature and decreasing particle concentration. At high particle concentrations, no f...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90383-8

    authors： Ausio J,Seger D,Eisenberg H

    更新日期：1984-06-15 00:00:00

  • Evaluating and learning from RNA pseudotorsional space: quantitative validation of a reduced representation for RNA structure.

    abstract：:Quantitatively describing RNA structure and conformational elements remains a formidable problem. Seven standard torsion angles and the sugar pucker are necessary to characterize the conformation of an RNA nucleotide completely. Progress has been made toward understanding the discrete nature of RNA structure, but clas...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.06.058

    authors： Wadley LM,Keating KS,Duarte CM,Pyle AM

    更新日期：2007-09-28 00:00:00

  • Identification of elements essential for transcription in Brugia malayi promoters.

    abstract：:Little is known concerning promoter structure in the filarial parasites. Recently, transient transfection methods have been developed for the human filarial parasite Brugia malayi. These methods have been employed to localize the promoter for the 70kDa heat shock protein (BmHSP70) to a region extending 394nt upstream ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.08.014

    authors： Higazi TB,Deoliveira A,Katholi CR,Shu L,Barchue J,Lisanby M,Unnasch TR

    更新日期：2005-10-14 00:00:00

  • Structural Insights into the Specific Recognition of 5-methylcytosine and 5-hydroxymethylcytosine by TAL Effectors.

    abstract：:Transcription activator-like effectors (TALEs) recognize DNA through repeat-variable diresidues (RVDs), and TALE-DNA interactions are sensitive to DNA modifications. Our previous study deciphered the recognition of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) by TALEs. Here, we report seven crystal struct...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.11.023

    authors： Liu L,Zhang Y,Liu M,Wei W,Yi C,Peng J

    更新日期：2020-02-14 00:00:00

  • Structure of a foreign peptide displayed on the surface of bacteriophage M13.

    abstract：:The use of filamentous bacteriophage M13 as a vehicle for display of foreign peptides and proteins provides a means for the construction of therapeutic, diagnostic and technological tools of broad utility. The usefulness of this technology is dependent on the ability of an inserted peptide to act as a ligand when fuse...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1489

    authors： Kishchenko G,Batliwala H,Makowski L

    更新日期：1994-08-12 00:00:00

  • Resolution of immobile chi structures by the FLP recombinase of 2 microns plasmid.

    abstract：:FLP is a conservative site-specific recombinase that is encoded by the 2 microns plasmid of the yeast, Saccharomyces cerevisiae. FLP is member of the integrase family of recombinases that mediate the recombination reaction through a Holliday intermediate. The FLP recognition target (FRT) sites lie within two 599 bp in...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1647

    authors： Dixon JE,Sadowski PD

    更新日期：1994-10-21 00:00:00

  • On the connection between inherent DNA flexure and preferred binding of hydroxymethyluracil-containing DNA by the type II DNA-binding protein TF1.

    abstract：:TF1 is a member of the family of type II DNA-binding proteins, which also includes the bacterial HU proteins and the Escherichia coli integration host factor (IHF). Distinctive to TF1, which is encoded by the Bacillus subtilis bacteriophage SPO1, is its preferential binding to DNA in which thymine is replaced by 5-hyd...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0392

    authors： Grove A,Galeone A,Mayol L,Geiduschek EP

    更新日期：1996-07-12 00:00:00