Abstract：
:Aggregation of proteins due to failure of quality control mechanisms is deleterious to both eukaryotes and prokaryotes. We found that in Escherichia coli, protein aggregates are delivered to the pole and form a large polar aggregate (LPA). The formation of LPAs involves two steps: the formation of multiple small aggregates and the delivery of these aggregates to the pole to form an LPA. Formation of randomly distributed aggregates, their delivery to the poles, and LPA formation are all energy-dependent processes. The latter steps require the proton motive force, activities of the DnaK and DnaJ chaperones, and MreB. About 90 min after their formation, the LPAs are dissolved in a process that is dependent upon ClpB, DnaK, and energy. Our results confirm and substantiate the notion that the formation of LPAs allows asymmetric inheritance of the aggregated proteins to a small number of daughter cells, enabling their rapid elimination from most of the bacterial population. Moreover, the results show that the processing of aggregated proteins by the protein quality control system is a multi-step process with distinct spatial and temporal controls.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Rokney A,Shagan M,Kessel M,Smith Y,Rosenshine I,Oppenheim ABdoi
10.1016/j.jmb.2009.07.009subject
Has Abstractpub_date
2009-09-25 00:00:00pages
589-601issue
3eissn
0022-2836issn
1089-8638pii
S0022-2836(09)00835-3journal_volume
392pub_type
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