The projection structure of the membrane protein microsomal glutathione transferase at 3 A resolution as determined from two-dimensional hexagonal crystals.

abstract：:The termination signal that limits transcription through the early region of bacteriophage T3 (T3Te) has been cloned and sequenced. The nucleotide sequence of T3Te is identical with that of T7Te, with the exception of a single G to U substitution in the 3' tail of the terminated transcript, and addition of an AC to th...

journal_title：Journal of molecular biology

authors： Briat JF,Bollag G,Kearney CA,Molineux I,Chamberlin MJ

更新日期：1987-11-05 00:00:00

abstract：:Factor H (FH) is a major complement control protein in serum. The seventh short complement regulator (SCR-7) domain of the 20 in FH is associated with age-related macular degeneration through a Tyr402His polymorphism. The recombinant SCR-6/8 domains containing either His402 or Tyr402 and their complexes with a heparin...

journal_title：Journal of molecular biology

authors： Fernando AN,Furtado PB,Clark SJ,Gilbert HE,Day AJ,Sim RB,Perkins SJ

更新日期：2007-04-27 00:00:00

abstract：:Type I restriction endonuclease holoenzymes contain methylase (M), restriction (R) and specificity (S) subunits, present in an M2:R2:S1 stoichiometry. These enzymes bind to specific DNA sequences and translocate dsDNA in an ATP-dependent manner toward the holoenzyme anchored at the recognition sequence. Once transloca...

journal_title：Journal of molecular biology

authors： Bianco PR,Hurley EM

更新日期：2005-09-30 00:00:00

abstract：:The crystal structure of cholesterol oxidase, a 56kDa flavoenzyme was anisotropically refined to 0.95A resolution. The final crystallographic R-factor and R(free) value is 11.0% and 13.2%, respectively. The quality of the electron density maps has enabled modeling of alternate conformations for 83 residues in the enzy...

journal_title：Journal of molecular biology

authors： Lario PI,Sampson N,Vrielink A

更新日期：2003-03-07 00:00:00

abstract：:Ferredoxin-NAD(P)(+) oxidoreductase (FNR) catalyzes the reduction of NAD(P)(+) to NAD(P)H with the reduced ferredoxin (Fd) during the final step of the photosynthetic electron transport chain. FNR from the green sulfur bacterium Chlorobaculum tepidum is functionally analogous to plant-type FNR but shares a structural ...

journal_title：Journal of molecular biology

authors： Muraki N,Seo D,Shiba T,Sakurai T,Kurisu G

更新日期：2010-08-20 00:00:00

abstract：:RNA polymerase transcribes certain genomic loci with higher errors rates. These transcription error-enriched genomic loci (TEELs) have implications in disease. Current deep-sequencing methods cannot distinguish TEELs from post-transcriptional modifications, stochastic transcription errors, and technical noise, impedin...

journal_title：Journal of molecular biology

authors： Cheung PP,Jiang B,Booth GT,Chong TH,Unarta IC,Wang Y,Suarez GD,Wang J,Lis JT,Huang X

更新日期：2020-06-12 00:00:00

abstract：:In this paper we describe a two-plasmid system which allows over-production of the R.EcoR124I restriction endonuclease. The endonuclease has been purified to homogeneity in milligram amounts and has been shown to be fully active for both restriction and modification. Unexpectedly, the enzyme was found to require only ...

journal_title：Journal of molecular biology

authors： Janscak P,Abadjieva A,Firman K

更新日期：1996-04-19 00:00:00

abstract：:Interactions among Bcl-2 family proteins are important for regulating apoptosis. Prosurvival members of the family interact with proapoptotic BH3 (Bcl-2-homology-3)-only members, inhibiting execution of cell death through the mitochondrial pathway. Structurally, this interaction is mediated by binding of the alpha-hel...

journal_title：Journal of molecular biology

authors： Dutta S,Gullá S,Chen TS,Fire E,Grant RA,Keating AE

更新日期：2010-05-21 00:00:00

abstract：:Thiol peroxidases (Tpxs) are dimeric 2-Cys peroxiredoxins from bacteria that preferentially reduce alkyl hydroperoxides. Catalysis requires two conserved residues, the peroxidatic cysteine and the resolving cysteine, which are located in helix alpha(2) and helix alpha(3), respectively. The partial unraveling of helice...

journal_title：Journal of molecular biology

authors： Hall A,Sankaran B,Poole LB,Karplus PA

更新日期：2009-11-06 00:00:00

abstract：:The domain organization of the zymogen subunits of the first component of human complement C1s, C1r2 and the complex C1s-C1r2-C1s was studied by electron microscopy. In the absence of Ca2+, monomeric C1s was visualized as a dumb-bell-shaped molecule consisting of two globular domains (center-to-center distance 11 nm) ...

journal_title：Journal of molecular biology

authors： Weiss V,Fauser C,Engel J

更新日期：1986-06-05 00:00:00

abstract：:We have isolated and sequenced a cloned complementary DNA insert complementary to the messenger RNA of a cytoplasmic actin expressed in human epidermal cells. This provides the first cytoplasmic actin complementary DNA sequence for a vertebrate organism. The actin amino acid sequence predicted from this complementary ...

journal_title：Journal of molecular biology

authors： Hanukoglu I,Tanese N,Fuchs E

更新日期：1983-02-05 00:00:00

abstract：:The effects of Ca(2+) binding on the side-chain methyl dynamics of calbindin D(9k) have been characterized by (2)H NMR relaxation rate measurements. Longitudinal, transverse in-phase, quadrupolar order, transverse anti-phase and double quantum relaxation rates are reported for both the apo and Ca(2+)-loaded states of ...

journal_title：Journal of molecular biology

authors： Johnson E,Chazin WJ,Rance M

更新日期：2006-04-07 00:00:00

abstract：:The Rev-erb family is an orphan nuclear receptor acting as a negative regulator of transcription. Rev-erbalpha and Rev-erbbeta are crucial components of the circadian clock and involved in various lipid homeostasis. They are unique nuclear receptors that lack the activation function 2 helix (AF2-helix) required for li...

journal_title：Journal of molecular biology

authors： Woo EJ,Jeong DG,Lim MY,Jun Kim S,Kim KJ,Yoon SM,Park BC,Ryu SE

更新日期：2007-10-26 00:00:00

abstract：:A pre-condition for the ab initio assignment of Euler angles to a set of projections from an asymmetric object is that at least three of the available projections correspond to rotations about different axes. For symmetric objects this condition may be relaxed. There are some applications of single-particle electron m...

journal_title：Journal of molecular biology

authors： Patwardhan A,Paul D,Al-Khayat HA,Morris EP

更新日期：2004-11-26 00:00:00

abstract：:A series of DNA heptadecamers containing the DNA analogues of RNA E-like 5'-d(GXA)/(AYG)-5' motifs (X/Y is complementary T/A, A/T, C/G, or G/C pair) were studied using nuclear magnetic resonance (NMR) methodology and distance geometry (DG)/molecular dynamics (MD) approaches. Such oligomers reveal excellent resolution ...

journal_title：Journal of molecular biology

authors： Chou SH,Chin KH

更新日期：2001-09-28 00:00:00

abstract：:Aldo-keto reductases (AKRs) are a large superfamily of NADPH-dependent enzymes that catalyze the reduction of aldehydes, aldoses, dicarbonyls, steroids, and monosaccharides. While their precise physiological role is generally unknown, AKRs are nevertheless involved in the detoxification of a broad range of toxic metab...

journal_title：Journal of molecular biology

authors： Scoble J,McAlister AD,Fulton Z,Troy S,Byres E,Vivian JP,Brammananth R,Wilce MC,Le Nours J,Zaker-Tabrizi L,Coppel RL,Crellin PK,Rossjohn J,Beddoe T

更新日期：2010-04-23 00:00:00

abstract：:Tumor suppressors p53, p63 and p73 comprise a family of stress-responsive transcription factors with distinct functions in development and tumor suppression. Most human cancers lose p53 function, yet all three proteins are capable of inducing apoptosis or cellular senescence. Mechanisms are therefore under investigati...

journal_title：Journal of molecular biology

authors： Canning P,von Delft F,Bullock AN

更新日期：2012-11-02 00:00:00

abstract：:The high-resolution structure of the N-terminal domain (NTD) of the retroviral capsid protein (CA) of Mason-Pfizer monkey virus (M-PMV), a member of the betaretrovirus family, has been determined by NMR. The M-PMV NTD CA structure is similar to the other retroviral capsid structures and is characterized by a six alpha...

journal_title：Journal of molecular biology

authors： Macek P,Chmelík J,Krízová I,Kaderávek P,Padrta P,Zídek L,Wildová M,Hadravová R,Chaloupková R,Pichová I,Ruml T,Rumlová M,Sklenár V

更新日期：2009-09-11 00:00:00

abstract：:To probe the mechanism of the catalytic antibody NPN43C9, we have constructed a three-dimensional model of the NPN43C9 variable region using our antibody structural database (ASD), which takes maximal advantage of immunoglobulin sequence and structural information. The ASD contains separately superimposed variable lig...

journal_title：Journal of molecular biology

authors： Roberts VA,Stewart J,Benkovic SJ,Getzoff ED

更新日期：1994-01-21 00:00:00

abstract：:We showed earlier that the mutation of Leu99 to alanine in bacteriophage T4 lysozyme creates an internal cavity of volume approximately 150 A(3) that binds benzene and a variety of other ligands. As such, this cavity provides an excellent target to study protein-ligand interaction. Here, we use low-temperature crystal...

journal_title：Journal of molecular biology

authors： Liu L,Baase WA,Matthews BW

更新日期：2009-01-16 00:00:00

abstract：:Prompted by the close relationship between tyrosine recombinases and type IB topoisomerases we have investigated the ability of human topoisomerase I to resolve the typical intermediate of recombinase catalysis, the Holliday junction. We demonstrate that human topoisomerase I catalyzes unidirectional resolution of a s...

journal_title：Journal of molecular biology

authors： Hede MS,Petersen RL,Frøhlich RF,Krüger D,Andersen FF,Andersen AH,Knudsen BR

更新日期：2007-01-26 00:00:00

abstract：:The crystal structure of glycosyltrehalose trehalohydrolase from the hyperthermophilic archaeum Sulfolobus solfataricus KM1 has been solved by multiple isomorphous replacement. The enzyme is an alpha-amylase (family 13) with unique exo-amylolytic activity for glycosyltrehalosides. It cleaves the alpha-1,4 glycosidic b...

journal_title：Journal of molecular biology

authors： Feese MD,Kato Y,Tamada T,Kato M,Komeda T,Miura Y,Hirose M,Hondo K,Kobayashi K,Kuroki R

更新日期：2000-08-11 00:00:00

abstract：:Electron microscopy and image processing of negatively stained crystalline sheets induced from Acanthamoeba actin have been used to yield a three-dimensional reconstruction of the actin molecule, including data to a maximum resolution of 15 A. This model shows actin to be an asymmetric, wedge-shaped molecule. A three-...

journal_title：Journal of molecular biology

authors： Smith PR,Fowler WE,Pollard TD,Aebi U

更新日期：1983-07-05 00:00:00

abstract：:We have identified Adrm1 as a novel component of the regulatory ATPase complex of the 26 S proteasome: Adrm1 was precipitated with an antibody to proteasomes and vice versa. Adrm1 co-migrated with proteasomes on gel-filtration chromatography and non-denaturing polyacrylamide gel electrophoresis. Adrm1 has been describ...

journal_title：Journal of molecular biology

authors： Jørgensen JP,Lauridsen AM,Kristensen P,Dissing K,Johnsen AH,Hendil KB,Hartmann-Petersen R

更新日期：2006-07-28 00:00:00

abstract：:We have mapped the origin of DNA replication (oriB) downstream of the 23 S rRNA gene in each copy of the inverted repeat (IR) of tobacco chloroplast DNA between positions 130,502 and 131,924 (IR(A)) by a combination of approaches. In vivo chloroplast DNA replication intermediates were examined by two-dimensional agaro...

journal_title：Journal of molecular biology

authors： Kunnimalaiyaan M,Shi F,Nielsen BL

更新日期：1997-05-02 00:00:00

abstract：:Gap junction channels are intercellular channels that form by docking the extracellular loops of connexin protein subunits. While the structure and function of gap junctions as intercellular channels have been characterized using different techniques, the physics of the inter-connexin interaction remain unknown. Moreo...

journal_title：Journal of molecular biology

authors： Rico F,Oshima A,Hinterdorfer P,Fujiyoshi Y,Scheuring S

更新日期：2011-09-09 00:00:00

abstract：:The crystal structure of muconate lactonizing enzyme has been solved at 3 A resolution, and an unambiguous alpha-carbon backbone chain trace made. The enzyme contains three domains; the central domain is a parallel-stranded alpha-beta barrel, which has previously been reported in six other enzymes, including triose ph...

journal_title：Journal of molecular biology

authors： Goldman A,Ollis DL,Steitz TA

更新日期：1987-03-05 00:00:00

abstract：:Peptides that bind to major histocompatibility complex products (MHC) are known to exhibit certain sequence motifs which, though common, are neither necessary nor sufficient for binding: MHCs bind certain peptides that do not have the characteristic motifs and only about 30% of the peptides having the required motif, ...

journal_title：Journal of molecular biology

authors： Gulukota K,Sidney J,Sette A,DeLisi C

更新日期：1997-04-18 00:00:00

abstract：:We study the early steps of amyloid formation of the seven residue peptide GNNQQNY from yeast prion-like protein Sup35 by simulating the random coil to beta-sheet and alpha-helix to beta-sheet transition both in the absence and presence of a cross-beta amyloid nucleus. The simulation method at atomic resolution employ...

journal_title：Journal of molecular biology

authors： Lipfert J,Franklin J,Wu F,Doniach S

更新日期：2005-06-10 00:00:00

abstract：:A novel gene from the nematode Caenorhabditis elegans was isolated by hybridization with a human calmodulin complementary DNA probe. This gene, cal-1, is present at one copy per haploid genome. In-situ hybridization of the cloned gene to metaphase chromosomes allowed us to assign it to the nematode linkage group IV. T...

journal_title：Journal of molecular biology

authors： Salvato M,Sulston J,Albertson D,Brenner S

更新日期：1986-08-05 00:00:00