A novel calmodulin-like gene from the nematode Caenorhabditis elegans.

abstract：:The 90-kDa heat shock proteins (Hsp90s) assist the maturation of many key regulators of signal transduction pathways and cellular control circuits like protein kinases and transcription factors and chaperone their stability and activity. In this function, Hsp90s cooperate with some 30 cochaperones and they are themsel...

journal_title：Journal of molecular biology

authors： Nguyen MTN,Knieß RA,Daturpalli S,Le Breton L,Ke X,Chen X,Mayer MP

更新日期：2017-03-10 00:00:00

abstract：:Viral scaffolding proteins direct polymerization of major capsid protein subunits into icosahedral procapsid structures. The scaffolding protein of bacteriophage SPP1 was engineered with a C-terminal hexahistidine tag (gp11-His(6)) and purified. The protein is an alpha-helical-rich molecule with a very elongated shape...

journal_title：Journal of molecular biology

authors： Poh SL,el Khadali F,Berrier C,Lurz R,Melki R,Tavares P

更新日期：2008-05-02 00:00:00

abstract：:Shikimate kinase (SK) and other enzymes in the shikimate pathway are potential targets for developing non-toxic antimicrobial agents, herbicides, and anti-parasite drugs, because the pathway is essential in the above species but is absent from mammals. The crystal structure of Mycobacterium tuberculosis SK (MtSK) in c...

journal_title：Journal of molecular biology

authors： Gu Y,Reshetnikova L,Li Y,Wu Y,Yan H,Singh S,Ji X

更新日期：2002-06-07 00:00:00

abstract：:Proteasomes, major proteolytic sites in eukaryotic cells, play an important part in major histocompatibility class I (MHC I) ligand generation and thus in the regulation of specific immune responses. Their cleavage specificity is of outstanding interest for this process. In order to generalize previously determined cl...

journal_title：Journal of molecular biology

authors： Kuttler C,Nussbaum AK,Dick TP,Rammensee HG,Schild H,Hadeler KP

更新日期：2000-05-05 00:00:00

abstract：:Members of the MarR family of winged helix transcriptional regulators have been shown to regulate multidrug and oxidative stress response, pathogenesis, and catabolism of aromatic compounds. Many respond to anionic lipophilic compounds in their capacity to bind DNA, and the co-crystal structure of MarR bound to salicy...

journal_title：Journal of molecular biology

authors： Wilkinson SP,Grove A

更新日期：2005-07-22 00:00:00

abstract：:Bacterial microcompartments (MCPs) are the simplest organelles known. They function to enhance metabolic pathways by confining several related enzymes inside an all-protein envelope called the shell. In this study, we investigated the factors that govern MCP assembly by performing scanning mutagenesis on the surface r...

journal_title：Journal of molecular biology

authors： Sinha S,Cheng S,Sung YW,McNamara DE,Sawaya MR,Yeates TO,Bobik TA

更新日期：2014-06-12 00:00:00

abstract：:A major problem in sequence alignments based on the standard dynamic programming method is that the optimal path does not necessarily yield the best equivalencing of residues assessed by structural or functional criteria. An algorithm is presented that finds suboptimal alignments of protein sequences by a simple modif...

journal_title：Journal of molecular biology

authors： Saqi MA,Sternberg MJ

更新日期：1991-06-20 00:00:00

abstract：:We have investigated the viscoelastic properties of the cytoplasmic intermediate filament (IF) proteins desmin and vimentin. Mechanical measurements were supported by time-dependent electron microscopy studies of the assembly process under similar conditions. Network formation starts within 2 min, but it takes more th...

journal_title：Journal of molecular biology

authors： Schopferer M,Bär H,Hochstein B,Sharma S,Mücke N,Herrmann H,Willenbacher N

更新日期：2009-04-24 00:00:00

abstract：:Addition of the antimitotic drug vinblastine to solutions of purified tubulin induces the formation of helical polymers whose structure and type of aggregation is determined by the concentration of magnesium. While paracrystalline arrangements of single coils are observed at low concentrations of the ion, for concentr...

journal_title：Journal of molecular biology

authors： Nogales E,Medrano FJ,Diakun GP,Mant GR,Towns-Andrews E,Bordas J

更新日期：1995-12-01 00:00:00

abstract：:Cytochrome c6from the unicellular green alga Scenedesmus obliquus was sequenced, crystallized in its reduced and oxidized state and the three-dimensional structure of the protein in both redox states was determined by X-ray crystallography. Reduced cytochrome c6crystallized as a monomer in the space group P 21212, whe...

journal_title：Journal of molecular biology

authors： Schnackenberg J,Than ME,Mann K,Wiegand G,Huber R,Reuter W

更新日期：1999-07-30 00:00:00

abstract：:The mammalian Sec61 complex forms a protein translocation channel whose function depends upon its interaction with the ribosome and with membrane proteins of the endoplasmic reticulum (ER). To study these interactions, we determined structures of "native" ribosome-channel complexes derived from ER membranes. We find t...

journal_title：Journal of molecular biology

authors： Ménétret JF,Hegde RS,Heinrich SU,Chandramouli P,Ludtke SJ,Rapoport TA,Akey CW

更新日期：2005-04-29 00:00:00

abstract：:The origin recognition complex (ORC), a possible initiator of chromosomal DNA replication in eukaryotes, binds to ATP through its subunits Orc1p and Orc5p. Orc1p possesses ATPase activity. As for DnaA, the Escherichia coli initiator, the ATP-DnaA complex is active but the ADP-DnaA complex is inactive for DNA replicati...

journal_title：Journal of molecular biology

authors： Takenaka H,Makise M,Kuwae W,Takahashi N,Tsuchiya T,Mizushima T

更新日期：2004-06-25 00:00:00

abstract：:The three-dimensional structure of cytochrome-c552 from Thermus thermophilus has been determined by the multiple anomalous dispersion technique using synchrotron radiation and refined to a resolution of 1.28 A. Data collection at 90 K and the recording of three data sets (f'-minimum: 7125 eV, f"-maximum: 7138 eV and r...

journal_title：Journal of molecular biology

authors： Than ME,Hof P,Huber R,Bourenkov GP,Bartunik HD,Buse G,Soulimane T

更新日期：1997-08-29 00:00:00

abstract：:Monoclonal antibodies that react with defined regions of the heavy and light chains of chicken skeletal muscle myosin have been used to provide a correlation between the primary and the tertiary structures of the head. Electron microscopy of rotary shadowed antibody-myosin complexes shows that the sites for three epit...

journal_title：Journal of molecular biology

authors： Winkelmann DA,Lowey S

更新日期：1986-04-20 00:00:00

abstract：:We describe experiments that enable us to track the presence and direction of the DNA bend induced by Escherichia coli catabolite activator protein (CAP) through the intermediate stages of transcription initiation at the lac promoter. Transcriptional complexes examined were formed on superhelical templates to enhance ...

journal_title：Journal of molecular biology

authors： Zinkel SS,Crothers DM

更新日期：1991-05-20 00:00:00

abstract：:Prion diseases are assumed to be caused by the infectious isoform, PrPsc, of a single cellular surface protein, PrPc. PrPsc is an insoluble form of PrPc and is believed to possess a different three-dimensional fold. It may propagate by causing PrPc to adopt its own infectious conformation by an unknown mechanism. Stud...

journal_title：Journal of molecular biology

authors： Hornemann S,Glockshuber R

更新日期：1996-09-06 00:00:00

abstract：:The helical coiled-coil region of the myosin rod in the nematode Caenorhabditis elegans is a repetitive sequence 1094 amino acids long which contains 39 repeats of a 28-residue pattern. The repeats are extremely significant when compared with the statistical distributions expected, first for random sequences, and then...

journal_title：Journal of molecular biology

authors： McLachlan AD

更新日期：1983-09-05 00:00:00

abstract：:HscA, a specialized bacterial Hsp70-class molecular chaperone, interacts with the iron-sulfur cluster assembly protein IscU by recognizing a conserved LPPVK sequence motif. We report the crystal structure of the substrate-binding domain of HscA (SBD, residues 389-616) from Escherichia coli bound to an IscU-derived pep...

journal_title：Journal of molecular biology

authors： Cupp-Vickery JR,Peterson JC,Ta DT,Vickery LE

更新日期：2004-09-24 00:00:00

abstract：:The role of the sigma 70 subunit of Escherichia coli RNA polymerase in transcription activation by positive transcription factors was investigated. For this purpose, we constructed a nested set of E. coli rpoD deletion mutants generating carboxy-terminally truncated sigma 70 subunits of RNA polymerase in a high-expres...

journal_title：Journal of molecular biology

authors： Kumar A,Grimes B,Fujita N,Makino K,Malloch RA,Hayward RS,Ishihama A

更新日期：1994-01-14 00:00:00

abstract：:The backbone dynamics of the cytokine interleukin-8, a symmetric homodimer of overall molecular mass 16 kDa, has been investigated at pH 5.2 by means of 15N relaxation measurements using heteronuclear two-dimensional inverse detected 1H-15N spectroscopy. 15N T1, T2 and NOE data were obtained for 66 out of a total of 6...

journal_title：Journal of molecular biology

authors： Grasberger BL,Gronenborn AM,Clore GM

更新日期：1993-03-20 00:00:00

abstract：:The role of electrostatic interactions in the stability and the folding of the N-terminal domain of the ribosomal protein L9 (NTL9) was investigated by determining the effects of varying the pH conditions. Urea denaturations and thermal unfolding experiments were used to measure the free energy of folding, DeltaG degr...

journal_title：Journal of molecular biology

authors： Luisi DL,Raleigh DP

更新日期：2000-06-16 00:00:00

abstract：:Rabbit liver aldolase was purified by affinity elution on a CM52 ion exchanger. Crystals of rabbit liver aldolase suitable for X-ray diffraction experiments were grown from 45% saturated ammonium sulfate solution at 4 degrees C. The enzyme crystallizes in space group C222(1) having cell dimensions a = 377.02 A, b = 13...

journal_title：Journal of molecular biology

authors： Sygusch J,Beaudry D

更新日期：1985-11-05 00:00:00

abstract：:The DNA sequences of the entire coding regions of the A and C type variable surface protein genes from Paramecium tetraurelia, stock 51 have been determined. The 8151 nucleotide open reading frame of the A gene contains several tandem repeats of 210 nucleotides within the central portion of the molecule as well as a p...

journal_title：Journal of molecular biology

authors： Nielsen E,You Y,Forney J

更新日期：1991-12-20 00:00:00

abstract：:Two-dimensional crystalline porin sheets were obtained by reconstitution of monodisperse protein trimers and phospholipids (dimyristoylphosphatidylcholine) by detergent dialysis, analogous to the reconstitution method used for functional tests (Schindler & Rosenbusch, 1981). Three different packing arrangements were o...

journal_title：Journal of molecular biology

authors： Dorset DL,Engel A,Häner M,Massalski A,Rosenbusch JP

更新日期：1983-04-25 00:00:00

abstract：:In order to systematically analyze functionally relevant dynamical correlations within macromolecular complexes, we have developed computational methods based on the normal mode analysis of an elastic network model. First, we define two types of dynamical correlations (fluctuation-based and density-based), which are c...

journal_title：Journal of molecular biology

authors： Zheng W,Brooks B

更新日期：2005-02-25 00:00:00

abstract：:P-type ATPases are involved in the active transport of ions across biological membranes. The KdpFABC complex (P-type ATPase) of Escherichia coli is a high-affinity K+ uptake system that operates only when the cell experiences osmotic stress or K+ limitation. Here, we present the solution structure of the nucleotide bi...

journal_title：Journal of molecular biology

authors： Haupt M,Bramkamp M,Coles M,Altendorf K,Kessler H

更新日期：2004-10-01 00:00:00

abstract：:A combination of enzyme kinetics and X-ray crystallographic analysis of site-specific mutants has been used to probe the determinants of substrate specificity for the enzyme alpha-lytic protease. We now present a generalized model for understanding the effects of mutagenesis on enzyme substrate specificity. This algor...

journal_title：Journal of molecular biology

authors： Wilson C,Mace JE,Agard DA

更新日期：1991-07-20 00:00:00

abstract：:Muscle-specific kinase (MuSK) is a receptor tyrosine kinase expressed exclusively in skeletal muscle, where it is required for formation of the neuromuscular junction. MuSK is activated by agrin, a neuron-derived heparan sulfate proteoglycan. Here, we report the crystal structure of the agrin-responsive first and seco...

journal_title：Journal of molecular biology

authors： Stiegler AL,Burden SJ,Hubbard SR

更新日期：2006-12-01 00:00:00

abstract：:The tyrosyl-tRNA synthetases from Bacillus stearothermophilus (Bst-TyrTS) and Escherichia coli (Eco-TyrTS) are 56% identical in amino acid sequence. To map and characterize the set of interactions that makes Bst-TyrTS more stable than Eco-TyrTS, a family of nine hybrid proteins was constructed between the two enzymes....

journal_title：Journal of molecular biology

authors： Guez-Ivanier V,Hermann M,Baldwin D,Bedouelle H

更新日期：1993-11-05 00:00:00

abstract：:Although DNA methylation is a fundamental mechanism for repressing genetic activity, the influence of methyl groups on DNA conformation is found to be small. In this study, the role of cytosine methylation is analysed in the context of DNA condensation by examining its influence on DNA-DNA recognition processes. Previ...

journal_title：Journal of molecular biology

authors： Mayer-Jung C,Moras D,Timsit Y

更新日期：1997-07-18 00:00:00