Preliminary crystallographic investigation of rabbit liver aldolase.

abstract：:Transcription of the Bacillus subtilis iol divergon is negatively regulated by a repressor encoded by iolR, which belongs to the DeoR family of bacterial regulators. Gel retardation analysis involving the IolR protein synthesized in Escherichia coli revealed that IolR bound specifically and independently to each of th...

journal_title：Journal of molecular biology

authors： Yoshida KI,Shibayama T,Aoyama D,Fujita Y

更新日期：1999-01-22 00:00:00

abstract：:The average structural and dynamic properties of tetragonal hen egg-white lysozyme have been compared, in structures refined at 1.9 A resolution, using data collected at 100 K and 298 K. The molecule expands by 1.8% over this temperature range with the expansion occurring primarily in its small sub-atomic-sized spaces...

journal_title：Journal of molecular biology

authors： Young AC,Tilton RF,Dewan JC

更新日期：1994-01-07 00:00:00

abstract：:Carboxypeptidase M (CPM), an extracellular glycosylphosphatidyl-inositol(GPI)-anchored membrane glycoprotein belonging to the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, specifically removes C-terminal basic residues from peptides and proteins. Due to its wide distribution in human tissues, CPM is belie...

journal_title：Journal of molecular biology

authors： Reverter D,Maskos K,Tan F,Skidgel RA,Bode W

更新日期：2004-04-23 00:00:00

abstract：:The DNA coding for the major outer membrane lipoprotein of Escherichia coli has been fused to the coding region of the beta-galactosidase gene to measure the effect of various mutations on the efficiency of translation initiation. The various mutants were made by either inserting or deleting a small number of nucleoti...

journal_title：Journal of molecular biology

authors： Coleman J,Inouye M,Nakamura K

更新日期：1985-01-05 00:00:00

abstract：:The rotavirus double-layered particle (DLP) is a molecular machine that transcribes 11 genomic segments of double-stranded RNA into full-length mRNA segments during viral replication. DLPs from the human Wa strain of virus, belonging to subgroup II (SG II), possess a significantly reduced level of transcriptase activi...

journal_title：Journal of molecular biology

authors： Greig SL,Berriman JA,O'Brien JA,Taylor JA,Bellamy AR,Yeager MJ,Mitra AK

更新日期：2006-02-10 00:00:00

abstract：:Escherichia coli pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the biosynthesis of pyridoxal 5'-phosphate by the FMN oxidation of pyridoxine 5'-phosphate forming FMNH(2) and H(2)O(2). Recent studies have shown that in addition to the active site, pyridoxine 5'-phosphate oxidase contains a non-catalyti...

journal_title：Journal of molecular biology

authors： Safo MK,Musayev FN,di Salvo ML,Schirch V

更新日期：2001-07-20 00:00:00

abstract：:The three-dimensional structure of the outer membrane protein A from Klebsiella pneumoniae transmembrane domain was determined by NMR.This protein induces specific humoral and cytotoxic responses, and is a potent carrier protein. This is one of the largest integral membrane proteins(210 residues) for which nearly comp...

journal_title：Journal of molecular biology

authors： Renault M,Saurel O,Czaplicki J,Demange P,Gervais V,Löhr F,Réat V,Piotto M,Milon A

更新日期：2009-01-09 00:00:00

abstract：:The basis for the choice of translational position of a histone octamer on DNA is poorly understood. To gain further insights into this question we have studied the translational and rotational settings of core particles assembled on a simple repeating 20 bp positioning sequence. We show that the translational positio...

journal_title：Journal of molecular biology

authors： Negri R,Buttinelli M,Panetta G,De Arcangelis V,Di Mauro E,Travers A

更新日期：2001-04-06 00:00:00

abstract：:The mom gene of bacteriophage Mu, which codes for a DNA modification function, is regulated in a complex manner at both transcriptional and translational levels. The phage-encoded C protein functions as an activator of mom transcription. The mom promoter has features of an activator-dependent weak promoter, and the C ...

journal_title：Journal of molecular biology

authors： Ramesh V,Nagaraja V

更新日期：1996-07-05 00:00:00

abstract：:Streptococcus mutans is the primary etiological agent of dental caries in man and other mammalian organisms. This bacterium metabolizes carbohydrates actively and thrives under anaerobic conditions by fermenting l-ascorbate (Asc) via the sga operon, which includes SgaT, PtxB, and PtxA. These three proteins are members...

journal_title：Journal of molecular biology

authors： Lei J,Li LF,Su XD

更新日期：2009-02-20 00:00:00

abstract：:The capsid protein of tomato bushy stunt virus (TBSV) has two antiparallel beta-sheet domains with the so-called jellyroll conformation. Contrary to previous analyses, we note that these domains are non-superimposable topologies. The TBSV shell (S) domain topology is common to many other proteins but the protruding (P...

journal_title：Journal of molecular biology

authors： Gibson TJ,Argos P

更新日期：1990-03-05 00:00:00

abstract：:We investigated the role of a partially folded intermediate that transiently accumulates during lysozyme folding. Previous studies had shown that the partially folded intermediate is located on a slow-folding pathway and that an additional fast direct pathway from the unfolded state to the native state exists. Kinetic...

journal_title：Journal of molecular biology

authors： Wildegger G,Kiefhaber T

更新日期：1997-07-11 00:00:00

abstract：:The sigma N class of sigma factors confer upon RNA polymerase the requirement for enhancer-binding activator proteins. The sigma-N (sigma N) protein of Klebsiella pneumoniae was analysed by the assay of purified peptides comprising domains or regions of sigma N defined by proteolysis or by homology alignment, respecti...

journal_title：Journal of molecular biology

authors： Cannon W,Missailidis S,Smith C,Cottier A,Austin S,Moore M,Buck M

更新日期：1995-05-12 00:00:00

abstract：:We demonstrate here that the Saccharomyces cerevisiae Mlh1-Pms1 heterodimer required for DNA mismatch repair and other cellular processes is a DNA binding protein. Binding was evaluated using a variety of single and double-stranded DNA molecules. Mlh1-Pms1 bound short substrates with low affinity and showed a slight p...

journal_title：Journal of molecular biology

authors： Hall MC,Wang H,Erie DA,Kunkel TA

更新日期：2001-09-28 00:00:00

abstract：:Plasmids were constructed in which a HindIII fragment of rDNA (6.4 x 10(3) base-pairs) was inserted into vectors pGEM-1 and 2 in both orientations. The DNA insert encoded the yeast 35 S precursor rRNA beginning 180 bases upstream from the 5' end of the mature 18 S rRNA and ending 289 bases beyond the 3' end of the mat...

journal_title：Journal of molecular biology

authors： Thweatt R,Lee JC

更新日期：1990-01-20 00:00:00

abstract：:Recent studies on iron-loaded transferrins have revealed a conformational change upon binding iron due to a domain closure. It has been suggested that the domain closure may be the key for the receptor recognition of the metal loaded transferrin (Grossmann et al., 1992). Small angle X-ray scattering has been used to p...

journal_title：Journal of molecular biology

authors： Grossmann JG,Neu M,Evans RW,Lindley PF,Appel H,Hasnain SS

更新日期：1993-02-05 00:00:00

abstract：:Pseudomonas aeruginosa encodes an enzyme (PA2794) that is annotated as a sialidase (or neuraminidase), as it possesses three bacterial neuraminidase repeats that are a signature of nonviral sialidases. A recent report showed that when the gene encoding this sialidase is knocked out, this led to a reduction in biofilm ...

journal_title：Journal of molecular biology

authors： Xu G,Ryan C,Kiefel MJ,Wilson JC,Taylor GL

更新日期：2009-02-27 00:00:00

abstract：:ThiI is an enzyme responsible for the formation of the modified base S(4)U (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This base acts as a sensitive trigger for the response mechanism to UV exposure, providing protection against its damaging effects. We present the crystal structure of Bacillus anth...

journal_title：Journal of molecular biology

authors： Waterman DG,Ortiz-Lombardía M,Fogg MJ,Koonin EV,Antson AA

更新日期：2006-02-10 00:00:00

abstract：:The restriction enzyme R.BbvCI cleaves duplex DNA within a seven base-pair asymmetric recognition sequence, thus: CCTCAGC/GCTGAGG-->CC--TCAGC/GC--TGAGG. We show that R.BbvCI comprises two different subunits, R(1) and R(2); that each subunit contains a catalytic site for DNA strand hydrolysis; and that these sites act ...

journal_title：Journal of molecular biology

authors： Heiter DF,Lunnen KD,Wilson GG

更新日期：2005-05-06 00:00:00

abstract：:We report the X-ray structure analysis and refinement at 1.9 A resolution of calf gamma-II crystallin, a lens-specific protein. The sequence of Croft (1972) has been modified to give a polypeptide chain of 174 residues (cf. 165). The protein has a symmetrical, hierarchical structure of two globular domains each compri...

journal_title：Journal of molecular biology

authors： Wistow G,Turnell B,Summers L,Slingsby C,Moss D,Miller L,Lindley P,Blundell T

更新日期：1983-10-15 00:00:00

abstract：:The sarcin/ricin domain in 23 S/28 S rRNA is crucial for ribosome function, since it constitutes at least part of the binding site for the elongation factors and hence is essential for binding aminoacyl-tRNA and for translocation. The domain is also the site of action of ricin and sarcin and analysis of the effect of ...

journal_title：Journal of molecular biology

authors： Macbeth MR,Wool IG

更新日期：1999-01-15 00:00:00

abstract：:Heterodimeric transcription regulatory proteins that bind palindromic DNA sequences can potentially bind their recognition sites in two opposite orientations. The orientation of transcription factor binding can control transcriptional activity by altering interactions with proteins that bind to adjacent regulatory ele...

journal_title：Journal of molecular biology

authors： Ramirez-Carrozzi VR,Kerppola TK

更新日期：2001-01-19 00:00:00

abstract：:The transforms of a large number of models of deoxygenated sickle hemoglobin fibers, related to that derived from image reconstruction of electron micrographs, have been calculated and compared with X-ray diffraction data of 15 A resolution. The model of the fiber, determined from the reconstructed image, is a helix c...

journal_title：Journal of molecular biology

authors： Rosen LS,Magdoff-Fairchild B

更新日期：1985-06-25 00:00:00

abstract：:Actin polymerization plays a major role in cell movement. The controls of actin sequestration/desequestration and of filament turnover are two important features of cell motility. Actin binding proteins use properties derived from the steady-state monomer-polymer cycle of actin in the presence of ATP, to control the F...

journal_title：Journal of molecular biology

authors： Carlier MF,Pantaloni D

更新日期：1997-06-20 00:00:00

abstract：:The 3' terminus of the (-) RNA strand in the replicative forms of several (+)-stranded RNA viruses possesses an unpaired guanosine with unknown function. This unpaired guanosine is also found at the 3' terminus of the (-) strand in the double-stranded form of two cucumoviral satellite RNAs. Using a cucumber mosaic vir...

journal_title：Journal of molecular biology

authors： Wu G,Kaper JM

更新日期：1994-05-20 00:00:00

abstract：:Crystals of an almost self-complementary DNA 15-mer d(CGCGAAATTTACGCG) have been grown by the vapor diffusion technique at 4 degrees C. The space group is I222 with a = 37.3 A, b = 54.6 A and c = 104.8 A. Solution studies showed that the 15-mer forms a duplex with the extra adenine residue unpaired: (sequence; see tex...

journal_title：Journal of molecular biology

authors： Miller M,Wlodawer A,Appella E,Sussman JL

更新日期：1987-06-20 00:00:00

abstract：:SnRK [SNF1 (sucrose non-fermenting-1)-related protein kinase] 2.6 [open stomata 1 (OST1)] is well characterized at molecular and physiological levels to control stomata closure in response to water-deficit stress. OST1 is a member of a family of 10 protein kinases from Arabidopsis thaliana (SnRK2) that integrates absc...

journal_title：Journal of molecular biology

authors： Yunta C,Martínez-Ripoll M,Zhu JK,Albert A

更新日期：2011-11-18 00:00:00

abstract：:Selenoproteins are present in all three domains of life and are responsible for a major part of a cell's antioxidant defense against reactive oxygen species. Synthesis of selenoproteins requires the decoding of a UGA codon as selenocysteine (Sec) instead of translation termination. Sec is incorporated into the growing...

journal_title：Journal of molecular biology

authors： Gupta N,DeMong LW,Banda S,Copeland PR

更新日期：2013-07-24 00:00:00

abstract：:Enzymes exhibit a strong long-range evolutionary constraint that extends from their catalytic site and affects even distant sites, where site-specific evolutionary rate increases monotonically with distance. While protein-protein sites in enzymes were previously shown to induce only a weak conservation gradient, a com...

journal_title：Journal of molecular biology

authors： Sharir-Ivry A,Xia Y

更新日期：2019-09-06 00:00:00

abstract：:The structure of the actin-myosin complex during ATP hydrolysis was studied by covalently crosslinking myosin subfragment 1 (S1) to F-actin in the presence of nucleotides (especially ATP) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The fluorescence energy transfer was measured between N-(iodoacetyl)-N'-(1-sul...

journal_title：Journal of molecular biology

authors： Arata T

更新日期：1986-09-05 00:00:00