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Isoform-Specific Phosphorylation in Human Hsp90β Affects Interaction with Clients and the Cochaperone Cdc37.

Abstract：

:The 90-kDa heat shock proteins (Hsp90s) assist the maturation of many key regulators of signal transduction pathways and cellular control circuits like protein kinases and transcription factors and chaperone their stability and activity. In this function, Hsp90s cooperate with some 30 cochaperones and they are themselves subject to regulation by numerous post-translational modifications. In vertebrates, two major isoforms exist in the cytosol, Hsp90α and Hsp90β, which share a high degree of sequence identity and are expressed in tissue- and environmental condition-dependent manner. We identified an isoform-specific phosphorylation site in human Hsp90β. This phosphorylation site seems to be linked to vertebrate evolution since it is not found in invertebrata but in all tetrapoda and many but not all fish species. We provide data suggesting that this phosphorylation is important for the activation of Hsp90 clients like glucocorticoid receptor and a protein kinase. Replacement of the phosphorylation site by glutamate affects the conformational dynamics of Hsp90 and interaction with the kinase-specific cochaperone Cdc37.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Nguyen MTN,Knieß RA,Daturpalli S,Le Breton L,Ke X,Chen X,Mayer MP

doi

10.1016/j.jmb.2017.01.011

subject

Has Abstract

pub_date

2017-03-10 00:00:00

pages

732-752

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(17)30039-6

journal_volume

429

pub_type

杂志文章

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