Preliminary crystallographic study of the Fab fragment of a monoclonal antibody directed against a cobra cardiotoxin.

abstract：:FF domains are small protein-protein interaction modules that have two flanking conserved phenylalanine residues. They are present in proteins involved in transcription, RNA splicing, and signal transduction, and often exist in tandem arrays. Although several individual FF domain structures have been determined by NMR...

journal_title：Journal of molecular biology

authors： Lu M,Yang J,Ren Z,Sabui S,Espejo A,Bedford MT,Jacobson RH,Jeruzalmi D,McMurray JS,Chen X

更新日期：2009-10-23 00:00:00

abstract：:The MotA protein of Escherichia coli is a component of the flagellum that functions, together with MotB, in transmembrane proton conduction. MotA and MotB are believed to form the stator of the flagellar motor. They are integral membrane proteins; MotA has a large (ca 22 kDa) domain in the cytoplasm, and MotB a much s...

journal_title：Journal of molecular biology

authors： Zhou J,Blair DF

更新日期：1997-10-24 00:00:00

abstract：:Pathogenic strains of Helicobacter pylori use a type IV secretion system (T4SS) to deliver the toxin CagA into human host cells. The T4SS, along with the toxin itself, is coded into a genomic insert, which is termed the cag pathogenicity island. The cag pathogenicity island contains about 30 open-reading frames, for m...

journal_title：Journal of molecular biology

authors： Cendron L,Couturier M,Angelini A,Barison N,Stein M,Zanotti G

更新日期：2009-02-13 00:00:00

abstract：:The Escherichia coli trigger factor is a peptidyl-prolyl cis/trans isomerase (PPIase) which catalyzes proline-limited protein folding extremely well. It has been found associated with nascent protein chains as well as with the chaperone GroEL. The trigger factor utilizes protein regions outside the central catalytic d...

journal_title：Journal of molecular biology

authors： Zarnt T,Tradler T,Stoller G,Scholz C,Schmid FX,Fischer G

更新日期：1997-09-05 00:00:00

abstract：:We have previously shown that in Escherichia coli the accumulation of five leucine and three methionine tRNA species is regulated so that those tRNA species that translate major codons increase while those that translate minor codons decrease as the growth rate increases. Here, we have analyzed the growth-rate-depende...

journal_title：Journal of molecular biology

authors： Emilsson V,Näslund AK,Kurland CG

更新日期：1993-03-20 00:00:00

abstract：:The MotA protein of Escherichia coli is a component of the flagella that functions together with the MotB protein in transmembrane proton conduction. It is an integral membrane protein, with four hydrophobic segments that might traverse the membrane and two short segments that are predicted to be in the periplasm. In ...

journal_title：Journal of molecular biology

authors： Zhou J,Fazzio RT,Blair DF

更新日期：1995-08-11 00:00:00

abstract：:A novel interrupted gene motif has been reported in which a 50-nucleotide insertion into bacteriophage T4 gene 60 appears to be present in the message at the time of translation, yet it is not translated. We present here a dynamic model for how translation may be occurring in the neighborhood of the interruption. The ...

journal_title：Journal of molecular biology

authors： Burke-Agüero DH,Hearst JE

更新日期：1990-05-20 00:00:00

abstract：:Fasciculin 2 and toxin alpha proteins belong to the same structural family of three-fingered snake toxins. They act on different targets, but in each case the binding region involves residues from loops I and II. The superimposition of the two structures suggests that these functional regions correspond to structurall...

journal_title：Journal of molecular biology

authors： Le Du MH,Ricciardi A,Khayati M,Ménez R,Boulain JC,Ménez A,Ducancel F

更新日期：2000-03-03 00:00:00

abstract：:The E. coli RecBCD enzyme facilitates the loading of RecA onto single-stranded DNA produced by the combined helicase/nuclease activity of RecBCD. The nuclease domain of RecB protein, RecB(nuc), has been previously shown to bind RecA. Surprisingly, RecB(nuc) also binds to phage and eukaryotic homologs of RecA, leading ...

journal_title：Journal of molecular biology

authors： Lucarelli D,Wang YA,Galkin VE,Yu X,Wigley DB,Egelman EH

更新日期：2009-08-14 00:00:00

abstract：:A large conformational change is observed between HIV-1 proteinase in the ligand-free state and in complexes with transition-state inhibitors. Crystal structures of this enzyme have either the flaps open for the native or ligand-free enzyme or the flaps closed for peptidomimetic ligand-bound enzyme. We describe the st...

journal_title：Journal of molecular biology

authors： Wilderspin AF,Sugrue RJ

更新日期：1994-05-27 00:00:00

abstract：:Human granulocyte colony-stimulating factor (hG-CSF) specifically stimulates proliferation of neutrophils. Two crystal forms of a mutant of hG-CSF expressed in Escherichia coli have been obtained using the hanging drop vapour diffusion method. One form is triclinic, space group P1, with cell dimensions a = 37.3 A, b =...

journal_title：Journal of molecular biology

authors： Nagahara Y,Konishi N,Yokoo Y,Hirayama N

更新日期：1990-07-05 00:00:00

abstract：:Lysine methylation is a key regulator of protein-protein binding. The amine group of lysine can accept up to three methyl groups, and experiments show that protein-protein binding free energies are sensitive to the extent of methylation. These sensitivities have been rationalized in terms of chemical and structural fe...

journal_title：Journal of molecular biology

authors： Rahman S,Wineman-Fisher V,Al-Hamdani Y,Tkatchenko A,Varma S

更新日期：2021-02-05 00:00:00

abstract：:To examine the previously proposed retroregulation model of spc mRNA degradation, two strains of Escherichia coli B/r were used; one has wild-type spc and lac operons and the other has a lac operon deletion, a wild-type spc operon, and a Pspc-rplN-lacZ fusion operon lacking the normal control sites of the spc operon (...

journal_title：Journal of molecular biology

authors： Liang ST,Ehrenberg M,Dennis P,Bremer H

更新日期：1999-05-14 00:00:00

abstract：:The ctc gene of Bacillus subtilis is transcribed in vitro by the minor RNA polymerase holoenzyme forms, E sigma 37 and E sigma 32. To study the expression and regulation of ctc in vivo, we constructed operon and translational fusions of the ctc promoter region to the lacZ gene of Escherichia coli. Our results indicate...

journal_title：Journal of molecular biology

authors： Igo MM,Losick R

更新日期：1986-10-20 00:00:00

abstract：:Co-operativity in ATP hydrolysis by GroEL can be described by a model in which each ring of GroEL is in equilibrium between a low (T) and high (R) affinity state for ATP. According to this model, the GroEL double-ring is in equilibrium between three states: TT, TR and RR. In order to find out which states bind non-fol...

journal_title：Journal of molecular biology

authors： Yifrach O,Horovitz A

更新日期：1996-01-26 00:00:00

abstract：:Several RNA-cleaving deoxyribozymes (DNAzymes) have been reported for efficient cleavage of purine-containing junctions, but none is able to efficiently cleave pyrimidine-pyrimidine (Pyr-Pyr) junctions. We hypothesize that a stronger Pyr-Pyr cleavage activity requires larger DNAzymes with complex structures that are d...

journal_title：Journal of molecular biology

authors： Lam JC,Withers JB,Li Y

更新日期：2010-07-23 00:00:00

abstract：:In both prokaryotic and eukaryotic organisms, repressors and activators are responsible for regulating gene expression. The lac operon is a paradigm for understanding how metabolites function as signaling molecules and modulate transcription. These metabolites or allosteric effector molecules bind to the repressor and...

journal_title：Journal of molecular biology

authors： Daber R,Sharp K,Lewis M

更新日期：2009-10-09 00:00:00

abstract：:We have analysed nucleotide sequences at the junction between simian virus 40 (SV40) and cellular DNA in the Fisher rat transformed line tsA30-N2. This line contains a single insertion of one complete SV40 genome with a terminal duplication of 267 nucleotides, the recombination sites being located at nucleotides 439 a...

journal_title：Journal of molecular biology

authors： Hasson JF,Mougneau E,Cuzin F,Yaniv M

更新日期：1984-07-25 00:00:00

abstract：:Transcription of the ribosomal RNA operons (rrn) in Escherichia coli is subject to an antitermination mechanism whereby RNA polymerase is modified to a termination-resistant form during transit through the rrn leader region. This antitermination mechanism is unable to overcome the T1-T2 terminator cluster located at t...

journal_title：Journal of molecular biology

authors： Ghosh B,Grzadzielska E,Bhattacharya P,Peralta E,DeVito J,Das A

更新日期：1991-11-05 00:00:00

abstract：:The coat protein of positive-stranded RNA viruses often contains a positively charged tail that extends toward the center of the capsid and interacts with the viral genome. Electrostatic interaction between the tail and the RNA has been postulated as a major force in virus assembly and stabilization. The goal of this ...

journal_title：Journal of molecular biology

authors： Ni P,Wang Z,Ma X,Das NC,Sokol P,Chiu W,Dragnea B,Hagan M,Kao CC

更新日期：2012-06-22 00:00:00

abstract：:We have investigated the viscoelastic properties of the cytoplasmic intermediate filament (IF) proteins desmin and vimentin. Mechanical measurements were supported by time-dependent electron microscopy studies of the assembly process under similar conditions. Network formation starts within 2 min, but it takes more th...

journal_title：Journal of molecular biology

authors： Schopferer M,Bär H,Hochstein B,Sharma S,Mücke N,Herrmann H,Willenbacher N

更新日期：2009-04-24 00:00:00

abstract：:Crystals of the apo form of the vitamin B12 and colicin receptor, BtuB, that diffract to 1.95 A have been grown by the membrane-based in meso technique. The structure of the protein differs in several details from that of its counterpart grown by the more traditional, detergent-based (in surfo) method. Some of these d...

journal_title：Journal of molecular biology

authors： Cherezov V,Yamashita E,Liu W,Zhalnina M,Cramer WA,Caffrey M

更新日期：2006-12-08 00:00:00

abstract：:Recently, we have found a transient intermediate on the folding pathway of src SH3. Intending to investigate the structure of the transient intermediate, we tested a mutant of src SH3, named A45G, using circular dichroism, fluorescence and X-ray solution scattering, and incidentally found that it forms a stable alpha-...

journal_title：Journal of molecular biology

authors： Li J,Matsumura Y,Shinjo M,Kojima M,Kihara H

更新日期：2007-09-21 00:00:00

abstract：:The telomeric d(GGGGTT).d(AACCCC) repeat tracts (G4T2 repeats) in Tetrahymena thermophila macronuclei were shown previously to be packaged in a non-nucleosomal DNA-protein complex. Here, we demonstrate that these telomeric repeats, together with a short region of the immediately adjacent non-telomeric sequence, exist ...

journal_title：Journal of molecular biology

authors： Cohen P,Blackburn EH

更新日期：1998-07-17 00:00:00

abstract：:P-glycoproteins, encoded by families of evolutionarily conserved genes, can confer a multidrug-resistant phenotype to mammalian tumor cells. To obtain more information on their functions in normal cells we have cloned genomic and complementary DNA sequences of four P-glycoprotein gene homologs of the genetically well-...

journal_title：Journal of molecular biology

authors： Lincke CR,The I,van Groenigen M,Borst P

更新日期：1992-11-20 00:00:00

abstract：:Eosinophil cationic protein (ECP; RNase 3) is a human ribonuclease found only in eosinophil leukocytes that belongs to the RNase A superfamily. This enzyme is bactericidal, helminthotoxic and cytotoxic to mammalian cells and tissues. The protein has been cloned, heterologously overexpressed, purified and crystallized....

journal_title：Journal of molecular biology

authors： Mallorquí-Fernández G,Pous J,Peracaula R,Aymamí J,Maeda T,Tada H,Yamada H,Seno M,de Llorens R,Gomis-Rüth FX,Coll M

更新日期：2000-07-28 00:00:00

abstract：:Using site-specific fluorescence probes and cross-linking we demonstrated that cofilin (ADF), a key regulator of actin cellular dynamics, weakens longitudinal contacts in F-actin in a cooperative manner. Differential scanning calorimetry detected a dual nature of cofilin effects on F-actin conformation. At sub-stoichi...

journal_title：Journal of molecular biology

authors： Bobkov AA,Muhlrad A,Pavlov DA,Kokabi K,Yilmaz A,Reisler E

更新日期：2006-02-17 00:00:00

abstract：:Human alpha-galactosidase A (alpha-D-galactoside galactohydrolase; EC 3.2.1.22), the glycosylated lysosomal enzyme deficient in Fabry disease, has been crystallized as a complex with the inhibitor N-6-aminohexanoyl-alpha-D-galactopyranosylamine. The "hanging drop" method of vapor diffusion was used to grow crystals fr...

journal_title：Journal of molecular biology

authors： Murali R,Ioannou YA,Desnick RJ,Burnett RM

更新日期：1994-06-17 00:00:00

abstract：:The control of DNA replication initiation in the plasmid ColE1 is mediated by RNAI, a 108 nucleotide plasmid-encoded RNA that is entirely complementary to the 5'-terminal region of the replication primer RNA. RNAI acts in trans to inhibit primer maturation. Previously, we constructed a plasmid in which the ColE1 RNAI ...

journal_title：Journal of molecular biology

authors： Dooley TP,Tamm J,Polisky B

更新日期：1985-11-05 00:00:00

abstract：:Homogeneous detergent-solubilized B873 light-harvesting complexes from a carotenoid-less mutant of the purple non-sulfur bacterium, Rhodospirillum rubrum G9, were reassembled spontaneously into two-dimensional (2D) hexagonal arrays during extensive and controlled dialysis. As the complexes contain only 1 to 2 mol phos...

journal_title：Journal of molecular biology

authors： Ghosh R,Hoenger A,Hardmeyer A,Mihailescu D,Bachofen R,Engel A,Rosenbusch JP

更新日期：1993-05-20 00:00:00