Isolation and characterization of mutants affecting functional domains of ColE1 RNAI.

abstract：:The solution structure of the cysteine-rich (CR) domain of Escherichia coli DnaJ has been solved by NMR methods. The structure of a 79 residue CR domain construct shows a novel fold with an overall V-shaped extended beta-hairpin topology. The CR domain is characterized by four C-X-X-C-X-G-X-G sequence motifs that bind...

journal_title：Journal of molecular biology

authors： Martinez-Yamout M,Legge GB,Zhang O,Wright PE,Dyson HJ

更新日期：2000-07-21 00:00:00

abstract：:Selenophosphate synthetase (SPS) catalyzes the activation of selenide with adenosine 5'-triphosphate (ATP) to generate selenophosphate, the essential reactive selenium donor for the formation of selenocysteine (Sec) and 2-selenouridine residues in proteins and RNAs, respectively. Many SPS are themselves Sec-containing...

journal_title：Journal of molecular biology

authors： Itoh Y,Sekine S,Matsumoto E,Akasaka R,Takemoto C,Shirouzu M,Yokoyama S

更新日期：2009-02-06 00:00:00

abstract：:We reported recently that activation of the inositol 1,4,5-triphosphate receptor (IP3R) is required for efficient HIV-1 Gag trafficking and viral particle release. IP3R activation requires phospholipase C (PLC)-catalyzed hydrolysis of PI(4,5)P(2) to IP3 and diacylglycerol. We show that Sprouty2 (Spry2), which binds PI...

journal_title：Journal of molecular biology

authors： Ehrlich LS,Medina GN,Carter CA

更新日期：2011-07-22 00:00:00

abstract：:6,7-Dimethyl-8-ribityllumazine synthase (lumazine synthase) catalyses the penultimate step in the biosynthesis of riboflavin. In Bacillus subtilis, 60 lumazine synthase subunits form an icosahedral capsid enclosing a homotrimeric riboflavin synthase unit. The ribH gene specifying the lumazine synthase subunit can be e...

journal_title：Journal of molecular biology

authors： Fischer M,Haase I,Kis K,Meining W,Ladenstein R,Cushman M,Schramek N,Huber R,Bacher A

更新日期：2003-02-21 00:00:00

abstract：:The chemotactic cytokine RANTES (Regulated on Activation, Normal T-cell Expressed and Secreted) is a potent chemoattractant and activator of a number of leukocytes, with a molecular mass of 8 kDa. Crystals of this protein have been grown from 100 mM sodium acetate buffer (pH 4.6) containing 200 mM magnesium acetate, w...

journal_title：Journal of molecular biology

authors： Shaw JP,Kryger G,Cleasby A,Wonacott A,Power CA,Proudfoot AE,Wells TN

更新日期：1994-09-30 00:00:00

abstract：:Saturation transfer electron paramagnetic resonance spectroscopy was used to investigate the rotational motion of the head domains of native and desensitized scallop myosin and its proteolytic subfragments. Scallop myosin was spin-labelled with 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidinooxyl, which reacted with ...

journal_title：Journal of molecular biology

authors： Wells C,Bagshaw CR

更新日期：1983-02-15 00:00:00

abstract：:Aquaporin-1 (AQP1) is an abundant protein in human erythrocyte membranes which functions as a specific and constitutively active water conducting pore. Solubilized and isolated as tetramer, it forms well-ordered two-dimensional (2D) crystals when reconstituted in the presence of lipids. Several high resolution project...

journal_title：Journal of molecular biology

authors： Walz T,Tittmann P,Fuchs KH,Müller DJ,Smith BL,Agre P,Gross H,Engel A

更新日期：1996-12-20 00:00:00

abstract：:The regulation of vertebrate striated muscle contraction involves a number of different molecules, including the thin-filament accessory proteins tropomyosin and troponin that provide Ca(2+)-dependent regulation by controlling access to myosin binding sites on actin. Cardiac myosin binding protein C (cMyBP-C) appears ...

journal_title：Journal of molecular biology

authors： Orlova A,Galkin VE,Jeffries CM,Egelman EH,Trewhella J

更新日期：2011-09-23 00:00:00

abstract：:We have examined the equilibrium and kinetic folding properties of two structurally homologous dihydrofolate reductases, Escherichia coli DHFR (EcDHFR) and murine DHFR (MuDHFR), as a function of temperature and ligand concentration. Conformational heterogeneity in native DHFR is well documented, and the results demons...

journal_title：Journal of molecular biology

authors： Clark AC,Frieden C

更新日期：1999-01-29 00:00:00

abstract：:The crystal structures of adenylate kinases from the thermophile Methanococcus thermolithotrophicus and the mesophile Methanococcus voltae have been solved to resolutions of 2.8A and 2.5A, respectively. The structures of the enzymes are similar to that of the adenylate kinase from archaeal Sulfolobus acidocaldarius in...

journal_title：Journal of molecular biology

authors： Criswell AR,Bae E,Stec B,Konisky J,Phillips GN Jr

更新日期：2003-07-25 00:00:00

abstract：:RNA virus behavior can be influenced by interactions among viral genomes and their expression products within the mutant spectra of replicating viral quasispecies. Here, we report the extent of interference of specific capsid and polymerase mutants of foot-and-mouth disease virus (FMDV) on replication of wild-type (wt...

journal_title：Journal of molecular biology

authors： Perales C,Mateo R,Mateu MG,Domingo E

更新日期：2007-06-15 00:00:00

abstract：:Replication of plasmid ColE1 is regulated by a plasmid-specified small RNA (RNA I). RNA I binds to the precursor (RNA II) of the primer for DNA synthesis and inhibits primer formation. The process of binding of RNA I to RNA II that results in formation of a stably bound complex consists of a series of reactions formin...

journal_title：Journal of molecular biology

authors： Tomizawa J

更新日期：1990-04-20 00:00:00

abstract：:The structure of the recombinant medium chain alcohol dehydrogenase (ADH) from the hyperthermophilic archaeon Aeropyrum pernix has been solved by the multiple anomalous dispersion technique using the signal from the naturally occurring zinc ions. The enzyme is a tetramer with 222 point group symmetry. The ADH monomer ...

journal_title：Journal of molecular biology

authors： Guy JE,Isupov MN,Littlechild JA

更新日期：2003-08-29 00:00:00

abstract：:Retroviral proteases are translated as a part of Gag-related polyproteins, and are released and activated during particle release. Mason-Pfizer monkey virus (M-PMV) Gag polyproteins assemble into immature capsids within the cytoplasm of the host cells; however, their processing occurs only after transport to the plasm...

journal_title：Journal of molecular biology

authors： Zábranská H,Tůma R,Kluh I,Svatos A,Ruml T,Hrabal R,Pichová I

更新日期：2007-02-02 00:00:00

abstract：:Single nucleotide changes to the sequence between two alternative 5' splice sites, separated by 25 nucleotides in a beta-globin gene derivative, caused substantial shifts in pre-mRNA splicing preferences, both in vivo and in vitro. An activating sequence for splicing was located. Models for the recognition by U1 small...

journal_title：Journal of molecular biology

authors： Cunningham SA,Else AJ,Potter BV,Eperon IC

更新日期：1991-01-20 00:00:00

abstract：:We have isolated and sequenced a cloned complementary DNA insert complementary to the messenger RNA of a cytoplasmic actin expressed in human epidermal cells. This provides the first cytoplasmic actin complementary DNA sequence for a vertebrate organism. The actin amino acid sequence predicted from this complementary ...

journal_title：Journal of molecular biology

authors： Hanukoglu I,Tanese N,Fuchs E

更新日期：1983-02-05 00:00:00

abstract：:A pseudoknot-containing aptamer isolated from a pool of random sequence molecules has been shown previously to represent an optimal RNA solution to the problem of binding biotin. The affinity of this RNA molecule is nonetheless orders of magnitude weaker than that of its highly evolved protein analogs, avidin and stre...

journal_title：Journal of molecular biology

authors： Nix J,Sussman D,Wilson C

更新日期：2000-03-10 00:00:00

abstract：:Carboxypeptidase M (CPM), an extracellular glycosylphosphatidyl-inositol(GPI)-anchored membrane glycoprotein belonging to the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, specifically removes C-terminal basic residues from peptides and proteins. Due to its wide distribution in human tissues, CPM is belie...

journal_title：Journal of molecular biology

authors： Reverter D,Maskos K,Tan F,Skidgel RA,Bode W

更新日期：2004-04-23 00:00:00

abstract：:The crystal structure of a class A beta-lactamase from Staphylococcus aureus PC1 has been refined at 2.0 A resolution. The resulting crystallographic R-factor (R = sigma h parallel Fo[-]Fc parallel/sigma h[Fo], where [Fo] and [Fc] are the observed and calculated structure factor amplitudes, respectively), is 0.163 for...

journal_title：Journal of molecular biology

authors： Herzberg O

更新日期：1991-02-20 00:00:00

abstract：:The ClpP component Clp protease from Escherichia coli has been crystallized and examined by X-ray crystallography and self-rotation function calculations. The crystal belongs to the monoclinic space group P2(1) with unit cell dimensions of a = 196.9 A, b = 104.3 A, c = 162.4 A and beta = 98.3 degrees. The X-ray diffra...

journal_title：Journal of molecular biology

authors： Shin DH,Lee CS,Chung CH,Suh SW

更新日期：1996-09-20 00:00:00

abstract：:Mature TGF-beta isoforms, which are covalent dimers, signal by binding to three types of cell surface receptors, the type I, II and III TGF-beta receptors. A complex composed of the TGF-beta ligand and the type I and II receptors is required for signaling. The type II receptor is responsible for recruiting TGF-beta in...

journal_title：Journal of molecular biology

authors： De Crescenzo G,Pham PL,Durocher Y,O'Connor-McCourt MD

更新日期：2003-05-16 00:00:00

abstract：:To define the structural basis for cofactor binding to membrane proteins, we introduce a manageable model system, which allows us, for the first time, to study the influence of individual transmembrane helices and of single amino acid residues on the assembly of a transmembrane cytochrome. In vivo as well as in vitro ...

journal_title：Journal of molecular biology

authors： Prodöhl A,Volkmer T,Finger C,Schneider D

更新日期：2005-07-22 00:00:00

abstract：:Like a variety of other bacteriophages, such as T4 and P22, bacteriophage P1 packages DNA by a "headful" mechanism in which the capacity of the viral capsid determines the size of the single DNA molecule that is packaged. Because of the long-standing and general acceptance of this packaging mechanism, we were surprise...

journal_title：Journal of molecular biology

authors： Coren JS,Pierce JC,Sternberg N

更新日期：1995-05-26 00:00:00

abstract：:The intergenic region in the genome of the Ff class of filamentous phage (comprising strains fl, fd and M13) genome constitutes 8% of the viral genome, and has essential functions in DNA replication and phage morphogenesis. The functional domains of this region may be inserted into separate sites of a plasmid to funct...

journal_title：Journal of molecular biology

authors： Specthrie L,Bullitt E,Horiuchi K,Model P,Russel M,Makowski L

更新日期：1992-12-05 00:00:00

abstract：:Lysine methylation is a key regulator of protein-protein binding. The amine group of lysine can accept up to three methyl groups, and experiments show that protein-protein binding free energies are sensitive to the extent of methylation. These sensitivities have been rationalized in terms of chemical and structural fe...

journal_title：Journal of molecular biology

authors： Rahman S,Wineman-Fisher V,Al-Hamdani Y,Tkatchenko A,Varma S

更新日期：2021-02-05 00:00:00

abstract：:New insights into the modular organization and flexibility of the N-terminal half of human cardiac myosin binding protein C (cMyBP-C) and information on the association state of the full-length protein have been deduced from a combined small-angle X-ray scattering (SAXS) and NMR study. SAXS data show that the first fi...

journal_title：Journal of molecular biology

authors： Jeffries CM,Lu Y,Hynson RM,Taylor JE,Ballesteros M,Kwan AH,Trewhella J

更新日期：2011-12-16 00:00:00

abstract：:Cyclin K and the closely related cyclins T1, T2a, and T2b interact with cyclin-dependent kinase 9 (CDK9) forming multiple nuclear complexes, referred to collectively as positive transcription elongation factor b (P-TEFb). Through phosphorylation of the C-terminal domain of the RNA polymerase II largest subunit, distin...

journal_title：Journal of molecular biology

authors： Baek K,Brown RS,Birrane G,Ladias JA

更新日期：2007-02-16 00:00:00

abstract：:A cytochrome c kinetic folding intermediate was studied by hydrogen exchange (HX) pulse labeling. Advances in the technique and analysis made it possible to define the structured and unstructured regions, equilibrium stability, and kinetic opening and closing rates, all at an amino acid-resolved level. The entire N-te...

journal_title：Journal of molecular biology

authors： Krishna MM,Lin Y,Mayne L,Englander SW

更新日期：2003-11-28 00:00:00

abstract：:Methods for the prediction of protein function from structure are of growing importance in the age of structural genomics. Here, we focus on the problem of identifying sites of potential serine protease inhibitor interactions on the surface of proteins of known structure. Given that there is no sequence conservation w...

journal_title：Journal of molecular biology

authors： Jackson RM,Russell RB

更新日期：2000-02-18 00:00:00

abstract：:The RuvAB and RecG proteins of Escherichia coli promote branch migration of Holliday junction intermediates in genetic recombination. Both are structure-specific helicases that unwind and rewind DNA at the junction point. The helicase activities of these proteins were investigated using RNA:DNA hybrid molecules. RuvAB...

journal_title：Journal of molecular biology

authors： Vincent SD,Mahdi AA,Lloyd RG

更新日期：1996-12-13 00:00:00