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Construction of a microphage variant of filamentous bacteriophage.

Abstract：

:The intergenic region in the genome of the Ff class of filamentous phage (comprising strains fl, fd and M13) genome constitutes 8% of the viral genome, and has essential functions in DNA replication and phage morphogenesis. The functional domains of this region may be inserted into separate sites of a plasmid to function independently. Here, we demonstrate the construction of a plasmid containing, sequentially, the origin of (+)-strand synthesis, the packaging signal and a terminator of (+)-strand synthesis. When host cells harboring this plasmid (pLS7) are infected with helper phage they produce a microphage particle containing all the structural elements of the mature, native phage. The microphage is 65 A in diameter and about 500 A long. It contains a 221-base single-stranded circle of DNA coated by about 95 copies of the major coat protein (gene 8 protein).

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Specthrie L,Bullitt E,Horiuchi K,Model P,Russel M,Makowski L

doi

10.1016/0022-2836(92)90858-h

keywords:

subject

Has Abstract

pub_date

1992-12-05 00:00:00

pages

720-4

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(92)90858-H

journal_volume

228

pub_type

杂志文章

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