当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > A shortened form of the Tetrahymena thermophila group I intron can catalyze the complete splicing reaction in trans.

A shortened form of the Tetrahymena thermophila group I intron can catalyze the complete splicing reaction in trans.

Abstract：

:The group I intron from Tetrahymena thermophila is able to catalyze its own excision from a precursor RNA. The intron recognizes the splice sites through an intron-encoded sequence called the internal guide sequence, or IGS. The 5' and 3' exons are thought to align on the IGS and form a pseudoknot structure consisting of two stems (P1 and P10). We created a shortened form of the intron that lacks the exon sequences and the entire IGS. This RNA is unable to react upon itself. It can catalyze a sequential two-step transesterification reaction on a P1P10 substrate added in trans that completely mimics splicing. The reaction works for different substrates that contain a U.G base-pair preceding the 5' cleavage site and a guanosine base preceding the 3' cleavage site, but that are otherwise unrelated in sequence. The ribozyme uses primarily the correct 5' and 3' splice sites even in the presence of potential cryptic splice sites, and therefore it must rely on the structure of the substrate (formation of the P1 and P10 helices) for correct splice site recognition. A C-G base-pair after the 5' splice site in P1 decreases activity while a U.G or G.U base-pair enhances activity. The relative position in P1 of the U.G base-pair preceding the 5' splice site is an important determinant. The ability of the intron to recognize primarily a specific structure, rather than a sequence, has ramifications for splice-site selection, for molecular modeling of the group I intron, and for ribozyme-based gene targeting.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Sargueil B,Tanner NK

doi

10.1006/jmbi.1993.1541

subject

Has Abstract

pub_date

1993-10-20 00:00:00

pages

629-43

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)71541-X

journal_volume

233

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Sequence analysis of the complete Caenorhabditis elegans myosin heavy chain gene family.

    abstract：:The sequences of three myosin heavy chain (MHC) genes from Caenorhabditis elegans, myo-1, 2 and 3, are presented. These genes, together with unc-54, comprise the entire nematode sacromeric MHC family. Comparison of nematode MHC sequences and sarcomeric, smooth and non-muscle MHCs from other organisms highlights conser...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90229-5

    authors： Dibb NJ,Maruyama IN,Krause M,Karn J

    更新日期：1989-02-05 00:00:00

  • Nucleosome binding by the bromodomain and PHD finger of the transcriptional cofactor p300.

    abstract：:The PHD finger and the bromodomain are small protein domains that occur in many proteins associated with phenomena related to chromatin. The bromodomain has been shown to bind acetylated lysine residues on histone tails. Lysine acetylation is one of several histone modifications that have been proposed to form the bas...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.01.051

    authors： Ragvin A,Valvatne H,Erdal S,Arskog V,Tufteland KR,Breen K,ØYan AM,Eberharter A,Gibson TJ,Becker PB,Aasland R

    更新日期：2004-04-02 00:00:00

  • Exploring the regulation of tRNA distribution on the genomic scale.

    abstract：:Though up to 20% of the total RNA in bacterial cells is tRNA, the regulation of tRNA distribution on the genomic level remains unclear. tRNA distribution is governed by four processes: transcription, processing of precursor tRNA, degradation of precursor tRNA and degradation of mature tRNA. To elucidate the relationsh...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.01.024

    authors： Dittmar KA,Mobley EM,Radek AJ,Pan T

    更新日期：2004-03-12 00:00:00

  • Developmental regulation of the aldolase A muscle-specific promoter during in vivo muscle maturation is controlled by a nuclear receptor binding element.

    abstract：:During the post-natal period, skeletal muscles undergo important modifications leading to the appearance of different types of myofibers which exhibit distinct contractile and metabolic properties. This maturation process results from the activation of the expression of different sets of contractile proteins and metab...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2821

    authors： Spitz F,Demignon J,Kahn A,Daegelen D,Maire P

    更新日期：1999-06-18 00:00:00

  • Ro ribonucleoprotein assembly in vitro. Identification of RNA-protein and protein-protein interactions.

    abstract：:The human Y RNAs, small RNAs with an unknown function, are complexed with at least three proteins: the 60,000 M(r) Ro protein (Ro60), the 52,000 M(r) Ro protein (Ro52) and the La protein (La). In this study we examined the intermolecular interactions between the components of these so-called Ro ribonucleoprotein (Ro R...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90890-v

    authors： Slobbe RL,Pluk W,van Venrooij WJ,Pruijn GJ

    更新日期：1992-09-20 00:00:00

  • Modular structure in developmentally eliminated DNA in Tetrahymena may be a consequence of frequent insertions and deletions.

    abstract：:The work reported here describes insertion-deletion (Indel) polymorphisms in two internally eliminated sequences (IESs, that are deleted during development in Tetrahymena): a 1.8 kb Indel at one end of the 1.1 kb H1 IES and a 0.5 kb Indel inside the 1.4 kb calmodulin (C) IES. These two IESs are located in the proximit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2003.12.065

    authors： Huvos P

    更新日期：2004-03-05 00:00:00

  • Deletion analysis of the lambda tR1 termination region. Effect of sequences near the transcript release sites, and the minimum length of rho-dependent transcripts.

    abstract：:In order to determine how much of the natural sequence is required for function of the lambda tR1 rho-dependent terminator, and to determine the minimum length required, we made two deletion series of a tR1 derivative that contains mostly foreign DNA, encoding C-rich RNA, substituted for the natural upstream sequences...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1229

    authors： Hart CM,Roberts JW

    更新日期：1994-04-01 00:00:00

  • Formation of a wrapped DNA-protein interface: experimental characterization and analysis of the large contributions of ions and water to the thermodynamics of binding IHF to H' DNA.

    abstract：:To characterize driving forces and driven processes in formation of a large-interface, wrapped protein-DNA complex analogous to the nucleosome, we have investigated the thermodynamics of binding the 34-base pair (bp) H' DNA sequence to the Escherichia coli DNA-remodeling protein integration host factor (IHF). Isotherm...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.11.104

    authors： Vander Meulen KA,Saecker RM,Record MT Jr

    更新日期：2008-03-14 00:00:00

  • Domain Interactions Determine the Amyloidogenicity of Antibody Light Chain Mutants.

    abstract：:In antibody light chain amyloidosis (AL), mutant light chains (LCs) or their variable domains (VLs) form fibrils, which accumulate in organs and lead to their failure. The molecular mechanism of this disease is still poorly understood. One of the key open issues is whether the mutant VLs and LCs differ in fibril forma...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.10.005

    authors： Weber B,Hora M,Kazman P,Pradhan T,Rührnößl F,Reif B,Buchner J

    更新日期：2020-11-20 00:00:00

  • Structure-guided identification of a laminin binding site on the laminin receptor precursor.

    abstract：:The 37/ 67-kDa human laminin receptor (LamR) is a cell surface receptor for laminin, prion protein, and a variety of viruses. Because of its wide range of ligands, LamR plays a role in numerous pathologies. LamR overexpression correlates with a highly invasive cell phenotype and increased metastatic ability, mediated ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2010.10.028

    authors： Jamieson KV,Hubbard SR,Meruelo D

    更新日期：2011-01-07 00:00:00

  • Topoisomerase II-mediated DNA cleavage: evidence for distinct regions of enzyme-DNA contacts.

    abstract：:To determine the specific interaction sites of topoisomerase II within the DNA region defined by the footprint of the enzyme, we have investigated the cleavage reaction on double-stranded DNA substrates containing nicks and deletions. Topoisomerase II-mediated cleavage of the DNA substrates is suicidal as the enzyme i...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0321

    authors： Alsner J,Sorensen HV,Schmidt VK,Sorensen BS,Westergaard O

    更新日期：1996-06-14 00:00:00

  • Structural mimicry of O-antigen by a peptide revealed in a complex with an antibody raised against Shigella flexneri serotype 2a.

    abstract：:The use of carbohydrate-mimicking peptides to induce immune responses against surface polysaccharides of pathogenic bacteria offers a novel approach to vaccine development. Factors governing antigenic and immunogenic mimicry, however, are complex and poorly understood. We have addressed this question using the anti-li...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.03.057

    authors： Theillet FX,Saul FA,Vulliez-Le Normand B,Hoos S,Felici F,Weintraub A,Mulard LA,Phalipon A,Delepierre M,Bentley GA

    更新日期：2009-05-15 00:00:00

  • Comprehensive mutagenesis of the C-terminal domain of the M13 gene-3 minor coat protein: the requirements for assembly into the bacteriophage particle.

    abstract：:Filamentous bacteriophage assemble at the host membrane in a non-lytic process; the gene-3 minor coat protein (P3) is required for release from the membrane and subsequently, for recognition and infection of a new host. P3 contains at least three distinct domains: two N-terminal domains that mediate host recognition a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00950-1

    authors： Weiss GA,Roth TA,Baldi PF,Sidhu SS

    更新日期：2003-09-26 00:00:00

  • Conservation of transition state structure in fast folding peripheral subunit-binding domains.

    abstract：:Phi-value analysis was used to characterise the structure of the transition state (TS) for folding of POB L146A Y166W, a peripheral subunit-binding domain that folds in microseconds. Helix 2 was structured in the TS with consolidating interactions from the structured loop that connects the two alpha-helices. This dist...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.06.081

    authors： Sharpe TD,Ferguson N,Johnson CM,Fersht AR

    更新日期：2008-10-31 00:00:00

  • Rules for coupled expression of regulator and effector genes in inducible circuits.

    abstract：:The induction of effector genes that encode enzymes is often controlled by the protein product of a regulator gene that is directly involved in the control of its own expression. This coupling of elementary gene circuits can lead to three patterns of regulator and effector gene expression. As effector gene expression ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0011

    authors： Hlavacek WS,Savageau MA

    更新日期：1996-01-12 00:00:00

  • Interaction of crystalline tyrosyl-tRNA synthetase with adenosine, adenosine monophosphate, adenosine triphosphate and pyrophosphate in the presence of tyrosinol.

    abstract：:Crystalline complexes of tyrosyl-tRNA synthetase from Bacillus stearothermophilus were prepared with adenosine, AMP, ATP and PPi, all in the presence of tyrosinol, which binds strongly to the tyrosine binding site but cannot be adenylated by ATP. The hydrolysis of ATP in the presence of crystalline tyrosyl-tRNA synthe...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90392-9

    authors： Monteilhet C,Blow DM,Brick P

    更新日期：1984-03-15 00:00:00

  • Thiol proteases. Comparative studies based on the high-resolution structures of papain and actinidin, and on amino acid sequence information for cathepsins B and H, and stem bromelain.

    abstract：:An accurate three-dimensional structure is known for papain (1.65 A resolution) and actinidin (1.7 A). A detailed comparison of these two structures was performed to determine the effect of amino acid changes on the conformation. It appeared that, despite only 48% identity in their amino acid sequence, different cryst...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(85)90348-1

    authors： Kamphuis IG,Drenth J,Baker EN

    更新日期：1985-03-20 00:00:00

  • Expressed gene clusters associated with cellular sensitivity and resistance towards anti-viral and anti-proliferative actions of interferon.

    abstract：:Interferons (IFN) are multi-functional proteins that induce a large number of genes which mediate many biological processes including host defense, cell growth control, signaling, and metabolism. Bioinformatics analysis of the 3'-untranslated regions of IFN-stimulated genes (ISGs) showed that the AU-rich elements (ARE...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.07.065

    authors： Khabar KS,Al-Haj L,Al-Zoghaibi F,Marie M,Dhalla M,Polyak SJ,Williams BR

    更新日期：2004-09-17 00:00:00

  • Structure of an escape mutant of glycoprotein N2 neuraminidase of influenza virus A/Tokyo/3/67 at 3 A.

    abstract：:The three-dimensional structure of the membrane glycoprotein neuraminidase of an escape mutant of the influenza virus strain A/Tokyo/3/67 has been determined to 3 A (1 A = 0.1 nm) resolution by X-ray diffraction. The mutant virus, selected by growing the virus in the presence of a monoclonal antibody to the neuraminid...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90344-0

    authors： Varghese JN,Webster RG,Laver WG,Colman PM

    更新日期：1988-03-05 00:00:00

  • Cysteine residue periodicity is a conserved structural feature of variable surface proteins from Paramecium tetraurelia.

    abstract：:The DNA sequences of the entire coding regions of the A and C type variable surface protein genes from Paramecium tetraurelia, stock 51 have been determined. The 8151 nucleotide open reading frame of the A gene contains several tandem repeats of 210 nucleotides within the central portion of the molecule as well as a p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90573-o

    authors： Nielsen E,You Y,Forney J

    更新日期：1991-12-20 00:00:00

  • Calorimetric analysis of binding of two consecutive DNA strands to RecA protein illuminates mechanism for recognition of homology.

    abstract：:RecA protein recognises two complementary DNA strands for homologous recombination. To gain insight into the molecular mechanism, the thermodynamic parameters of the DNA binding have been characterised by isothermal calorimetry. Specifically, conformational changes of protein and DNA were searched for by measuring var...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.10.042

    authors： Takahashi M,Maraboeuf F,Morimatsu K,Selmane T,Fleury F,Norden B

    更新日期：2007-01-19 00:00:00

  • Domain closure mechanism in transferrins: new viewpoints about the hinge structure and motion as deduced from high resolution crystal structures of ovotransferrin N-lobe.

    abstract：:The crystal structure of holo hen ovotransferrin N-lobe refined at 1.65 A resolution has been obtained. The final model gave an R-factor of 0.173 in the resolution range between 10.0 and 1.65 A. The comparison of the structure with previous high-resolution apo and Fe(3+)-loaded, domain-opened intermediate structures p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4719

    authors： Mizutani K,Mikami B,Hirose M

    更新日期：2001-06-15 00:00:00

  • Structural and biophysical characterization of the Syk activation switch.

    abstract：:Syk is an essential non-receptor tyrosine kinase in intracellular immunological signaling, and the control of Syk kinase function is considered as a valuable target for pharmacological intervention in autoimmune or inflammation diseases. Upon immune receptor stimulation, the kinase activity of Syk is regulated by bind...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.11.007

    authors： Grädler U,Schwarz D,Dresing V,Musil D,Bomke J,Frech M,Greiner H,Jäkel S,Rysiok T,Müller-Pompalla D,Wegener A

    更新日期：2013-01-23 00:00:00

  • A conserved insertion in protein-primed DNA polymerases is involved in primer terminus stabilisation.

    abstract：:Protein-primed DNA polymerases form a subgroup of the eukaryotic-type DNA polymerases family, also called family B or alpha-like. A multiple amino acid sequence alignment of this subgroup of DNA polymerases led to the identification of two insertions, TPR-1 and TPR-2, in the polymerisation domain. We showed previously...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00788-5

    authors： Dufour E,Rodríguez I,Lázaro JM,de Vega M,Salas M

    更新日期：2003-08-22 00:00:00

  • RpoD promoters in Campylobacter jejuni exhibit a strong periodic signal instead of a -35 box.

    abstract：:We have used a hidden Markov model (HMM) to identify the consensus sequence of the RpoD promoters in the genome of Campylobacter jejuni. The identified promoter consensus sequence is unusual compared to other bacteria, in that the region upstream of the TATA-box does not contain a conserved -35 region, but shows a ver...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00034-2

    authors： Petersen L,Larsen TS,Ussery DW,On SL,Krogh A

    更新日期：2003-03-07 00:00:00

  • One is not enough.

    abstract：:In both prokaryotic and eukaryotic organisms, repressors and activators are responsible for regulating gene expression. The lac operon is a paradigm for understanding how metabolites function as signaling molecules and modulate transcription. These metabolites or allosteric effector molecules bind to the repressor and...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.07.050

    authors： Daber R,Sharp K,Lewis M

    更新日期：2009-10-09 00:00:00

  • Normal mode refinement: crystallographic refinement of protein dynamic structure. I. Theory and test by simulated diffraction data.

    abstract：:A dynamic structure refinement method for X-ray crystallography, referred to as the normal mode refinement, is proposed. The Debye-Waller factor is expanded in terms of the low-frequency normal modes whose amplitudes and eigenvectors are experimentally optimized in the process of the crystallographic refinement. In th...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90932-a

    authors： Kidera A,Go N

    更新日期：1992-05-20 00:00:00

  • Use of site-specific recombination as a probe of DNA structure and metabolism in vivo.

    abstract：:We used site-specific recombination catalyzed by the bacteriophage lambda Int system to probe DNA structure and metabolism in vivo. In vitro, the complexity of catenated products was linearly proportional to substrate supercoil density. A system was developed that gave efficient, controlled Int recombination in Escher...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90369-x

    authors： Bliska JB,Cozzarelli NR

    更新日期：1987-03-20 00:00:00

  • Expression and structural analysis of 14-3-3 proteins.

    abstract：:The 14-3-3 family of proteins plays a role in a wide variety of cellular functions including regulation of protein kinase C and exocytosis. Using antisera specific for the N termini of 14-3-3 isoforms described previously and an additional antiserum specific for the C terminus of epsilon isoform, protease digestion of...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.0031

    authors： Jones DH,Martin H,Madrazo J,Robinson KA,Nielsen P,Roseboom PH,Patel Y,Howell SA,Aitken A

    更新日期：1995-01-27 00:00:00

  • Conformational changes in the ribosome induced by translational miscoding agents.

    abstract：:Ribosomes are dynamic complexes responsible for translating the genetic information encoded in mRNAs to proteins. The accuracy of this process is vital to the survival of an organism, and is often compromised by translational miscoding agents. Aminoglycosides are a group of miscoding agents that bind to the ribosome a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4269

    authors： Jerinic O,Joseph S

    更新日期：2000-12-15 00:00:00