Sequence analysis of the complete Caenorhabditis elegans myosin heavy chain gene family.

abstract：:Rat intestinal fatty-acid-binding protein (I-FABP) is a small (15,124 Mr) cytoplasmic polypeptide that binds long-chain fatty acids in a non-covalent fashion. I-FABP is a member of a family of intracellular binding proteins that are thought to participate in the uptake, transport and/or metabolic targeting of hydropho...

journal_title：Journal of molecular biology

authors： Sacchettini JC,Gordon JI,Banaszak LJ

更新日期：1989-07-20 00:00:00

abstract：:Through the high-resolution structure of the gramicidin A channel in lamellar phase lipids and the characterization of specific ion peptide interactions, fundamental principles for ion channel selectivity and conductance efficiency are illustrated with atomic resolution detail. Delocalized cation binding in the first ...

journal_title：Journal of molecular biology

authors： Tian F,Cross TA

更新日期：1999-02-05 00:00:00

abstract：:RNA polymerase transcribes certain genomic loci with higher errors rates. These transcription error-enriched genomic loci (TEELs) have implications in disease. Current deep-sequencing methods cannot distinguish TEELs from post-transcriptional modifications, stochastic transcription errors, and technical noise, impedin...

journal_title：Journal of molecular biology

authors： Cheung PP,Jiang B,Booth GT,Chong TH,Unarta IC,Wang Y,Suarez GD,Wang J,Lis JT,Huang X

更新日期：2020-06-12 00:00:00

abstract：:The Sto12a protein, from the thermoacidophilic archaeon Sulfolobus tokodaii, has been identified as a small putative DNA-binding protein. Most of the proteins with a high level of amino acid sequence homology to this protein are derived from members of the Sulfolobaceae family, including a transcriptional regulator. W...

journal_title：Journal of molecular biology

authors： Shinkai A,Sekine S,Urushibata A,Terada T,Shirouzu M,Yokoyama S

更新日期：2007-10-05 00:00:00

abstract：:An in vitro assay in which terminating Escherichia coli ribosomes with different stop signals in the A-site compete for a limited amount of a release factor (RF1 or RF2) has been used to estimate the relative termination efficiencies at stop codons with different adjacent downstream nucleotides. The assay allows direc...

journal_title：Journal of molecular biology

authors： Pavlov MY,Freistroffer DV,Dincbas V,MacDougall J,Buckingham RH,Ehrenberg M

更新日期：1998-12-04 00:00:00

abstract：:The mutants P235A and F236A have been generated and their crystal structure was determined to resolutions of 2.38 and 2.35 A, respectively, in order to understand the residues involved in the formation of the novel arched P-loop of subunit A of the A-ATP synthase from Pyrococcus horikoshii OT3. Both the structures sho...

journal_title：Journal of molecular biology

authors： Kumar A,Manimekalai MS,Balakrishna AM,Priya R,Biuković G,Jeyakanthan J,Grüber G

更新日期：2010-09-03 00:00:00

abstract：:A long-standing goal in biology is the complete annotation of function and structure on all protein-protein interactions, a large fraction of which is mediated by intrinsically disordered protein regions (IDRs). However, knowledge derived from experimental structures of such protein complexes is disproportionately sma...

journal_title：Journal of molecular biology

authors： Wong ETC,Gsponer J

更新日期：2019-08-09 00:00:00

abstract：:The immI region of bacteriophage P1 includes the ant/reb gene, which encodes the antirepressor protein, and the c4 gene, which encodes a repressor molecule that negatively regulates antirepressor synthesis. The antirepressor interferes with the activity of the P1 repressor of lytic function, the product of the c1 gene...

journal_title：Journal of molecular biology

authors： Heisig A,Riedel HD,Dobrinski B,Lurz R,Schuster H

更新日期：1989-10-20 00:00:00

abstract：:Bovine enterovirus (BEV) strain VG-5-27 derived from an infectious cDNA clone has been crystallized as extended hexagonal plates. Virus recovered from crystals produced cytopathic effect in BHK cells. These crystals diffract X-rays from high energy synchrotron sources to beyond 2.7 A. The crystal system is monoclinic,...

journal_title：Journal of molecular biology

authors： Smyth M,Fry E,Stuart D,Lyons C,Hoey E,Martin SJ

更新日期：1993-06-05 00:00:00

abstract：:Approximately one-third of the human proteome is made up of proteins that are entirely disordered or that contain extended disordered regions. Although these disordered proteins are closely linked with many major diseases, their binding mechanisms with small molecules remain poorly understood, and a major concern is w...

journal_title：Journal of molecular biology

authors： Heller GT,Aprile FA,Bonomi M,Camilloni C,De Simone A,Vendruscolo M

更新日期：2017-09-01 00:00:00

abstract：:The equilibrium and kinetic folding properties of a small oncogene product, P13(MTCP1), of novel topology have been investigated using perturbation by guanidine hydrochloride and observation by fluorescence, circular dichroism and two-dimensional heteronuclear NMR spectroscopy. The structure of P13(MTCP1) is comprised...

journal_title：Journal of molecular biology

authors： Roumestand C,Boyer M,Guignard L,Barthe P,Royer CA

更新日期：2001-09-07 00:00:00

abstract：:The three-dimensional structure of the bacteriophage T7 head-to-tail connector has been obtained at 8A resolution using cryo-electron microscopy and single-particle analysis from purified recombinant connectors. The general morphology of the T7 connector is that of a 12-folded toroidal homopolymer with a channel that ...

journal_title：Journal of molecular biology

authors： Agirrezabala X,Martín-Benito J,Valle M,González JM,Valencia A,Valpuesta JM,Carrascosa JL

更新日期：2005-04-15 00:00:00

abstract：:We have previously isolated sphericase (Sph), an extracellular mesophilic serine protease produced by Bacillus sphaericus. The Sph amino acid sequence is highly homologous to two cold-adapted subtilisins from Antarctic bacilli S39 and S41 (76% and 74% identity, respectively). Sph is calcium-dependent, 310 amino acid r...

journal_title：Journal of molecular biology

authors： Almog O,González A,Klein D,Greenblatt HM,Braun S,Shoham G

更新日期：2003-10-03 00:00:00

abstract：:Arenaviruses are enveloped, negative-stranded RNA viruses that belong to the family Arenaviridae. This diverse family can be further classified into OW (Old World) and NW (New World) arenaviruses based on their antigenicity, phylogeny, and geographical distribution. Many of the NW arenaviruses are highly pathogenic vi...

journal_title：Journal of molecular biology

authors： Koma T,Huang C,Kolokoltsova OA,Brasier AR,Paessler S

更新日期：2013-12-13 00:00:00

abstract：:A new statistic Sc, which has a number of advantages over other measures of packing, is used to examine the shape complementarity of protein/protein interfaces selected from the Brookhaven Protein Data Bank. It is shown using Sc that antibody/antigen interfaces as a whole exhibit poorer shape complementarity than is o...

journal_title：Journal of molecular biology

authors： Lawrence MC,Colman PM

更新日期：1993-12-20 00:00:00

abstract：:Using HU chemical nucleases to probe HU-DNA interactions, we report here for the first time site-specific binding of HU to naked DNA. An unique feature of this interaction is the absolute requirement for negative DNA supercoiling for detectable levels of site-specific DNA binding. The HU binding site is the Mu spacer ...

journal_title：Journal of molecular biology

authors： Kobryn K,Lavoie BD,Chaconas G

更新日期：1999-06-18 00:00:00

abstract：:Protein engineering experiments and Phi(F)-value analysis of SH3 domains reveal that their transition state ensemble (TSE) is conformationally restricted, i.e. the fluctuations in the transition state (TS) structures are small. In the TS of src SH3 and alpha-spectrin SH3 the distal loop and the associated hairpin are ...

journal_title：Journal of molecular biology

authors： Klimov DK,Thirumalai D

更新日期：2002-04-12 00:00:00

abstract：:Phosphorylation of short linear peptide motifs is a widespread process for the dynamic regulation of protein-protein interactions. However, the global impact of phosphorylation events on the protein-protein interactome is rarely addressed. The disordered C-terminal tail of ribosomal S6 kinase 1 (RSK1) binds to PDZ dom...

journal_title：Journal of molecular biology

authors： Gógl G,Biri-Kovács B,Durbesson F,Jane P,Nomine Y,Kostmann C,Bilics V,Simon M,Reményi A,Vincentelli R,Trave G,Nyitray L

更新日期：2019-03-15 00:00:00

abstract：:To date, the literature concerning the HS1 (haematopoietic cell-specific protein 1)-associated protein X-1 (HAX1) protein has reported considerable variation regarding its function in mammalian cells, subcellular localisation and binding partners. We show here that HAX1 comprises a family of proteins. Murine tissues e...

journal_title：Journal of molecular biology

authors： Lees DM,Hart IR,Marshall JF

更新日期：2008-06-13 00:00:00

abstract：:Certain pathogenic trypanosomatids are highly dependent on glycolysis for ATP production, and hence their glycolytic enzymes, including glycerol-3-phosphate dehydrogenase (GPDH), are considered attractive drug targets. The ternary complex structure of Leishmania mexicana GPDH (LmGPDH) with dihydroxyacetone phosphate (...

journal_title：Journal of molecular biology

authors： Choe J,Guerra D,Michels PA,Hol WG

更新日期：2003-05-30 00:00:00

abstract：:A map of the 30 S ribosomal subunit is presented giving the positions of 15 of its 21 proteins. The components located in the map are S1, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S14, S15, S18 and S20. ...

journal_title：Journal of molecular biology

authors： Ramakrishnan V,Capel M,Kjeldgaard M,Engelman DM,Moore PB

更新日期：1984-04-05 00:00:00

abstract：:The cytokine interleukin 13 (IL-13) is a major effector molecule for T-helper type 2 inflammation and is pathogenic in allergic diseases such as asthma. The effects of IL-13 are mediated via a pathway that is initiated by binding to a heterodimeric receptor consisting of IL-13Rα1 and IL-4Rα. Antibodies raised against ...

journal_title：Journal of molecular biology

authors： Ultsch M,Bevers J,Nakamura G,Vandlen R,Kelley RF,Wu LC,Eigenbrot C

更新日期：2013-04-26 00:00:00

abstract：:Several extracellular modular proteins, including proteases of the complement and blood coagulation cascades, are shown here to exhibit conserved sequence patterns specific for a particular module-domain association. This was detected by comparative analysis of sequence variability in different multiple sequence align...

journal_title：Journal of molecular biology

authors： Gaboriaud C,Rossi V,Fontecilla-Camps JC,Arlaud GJ

更新日期：1998-09-18 00:00:00

abstract：:The Integrases of bacteriophages lambda and HK022 promote recombination between DNA molecules that carry attachment sites. The two integrases are about 70% identical in sequence and catalyze nearly identical reactions, but recognize different sets of sites. To identify the amino acids that determine this difference in...

journal_title：Journal of molecular biology

authors： Dorgai L,Yagil E,Weisberg RA

更新日期：1995-09-15 00:00:00

abstract：:Mammalian ferritins are 24-meric proteins composed of variable proportions of H and L-subunits. The L-chain, in contrast to the H-chain, lacks detectable ferroxidase activity, and its role in ferritin iron incorporation is unclear. In this study, apoferritins were subjected to iron loading with large iron increments t...

journal_title：Journal of molecular biology

authors： Levi S,Santambrogio P,Cozzi A,Rovida E,Corsi B,Tamborini E,Spada S,Albertini A,Arosio P

更新日期：1994-05-20 00:00:00

abstract：:DNA polymerases that initiate replication by protein-priming are able to catalyze terminal protein (TP)-primed initiation, the following transition steps and finally DNA-primed elongation. Therefore, their structures must be able to position sequentially both primers, TP and DNA, at a common binding site. For DNA-temp...

journal_title：Journal of molecular biology

authors： Truniger V,Lázaro JM,Blanco L,Salas M

更新日期：2002-04-19 00:00:00

abstract：:Chemical modifications, NES-Cys(beta 93), des-Arg(alpha 141), and both modifications on the same molecule, were made to Ni-Fe hybrid hemoglobins, and their effect on individual subunits was investigated by measuring oxygen equilibrium curves, the Fe(II)-N epsilon (His F8) stretching Raman lines, and light-absorption s...

journal_title：Journal of molecular biology

authors： Shibayama N,Morimoto H,Kitagawa T

更新日期：1986-11-20 00:00:00

abstract：:Restriction fragments of bacteriophage lambda DNA corresponding to the major leftward transcription unit were purified, denatured to form single-stranded DNA, self-annealed, and examined by electron microscopy. Three intrastrand stem and loop secondary structures were observed reproducibly and the locations of the pai...

journal_title：Journal of molecular biology

authors： Edlind TD,Cooley TE,Richards SH,Ihler GM

更新日期：1984-11-05 00:00:00

abstract：:SpoIIAA is a key component in the network of interactions that regulate the first sporulation-specific transcription factor, sigma(F), in Bacillus subtilis. In its unphosphorylated form SpoIIAA is either phosphorylated by or forms a non-covalent complex with SpoIIAB, whereas in its phosphorylated form it is dephosphor...

journal_title：Journal of molecular biology

authors： Clarkson J,Campbell ID,Yudkin MD

更新日期：2001-11-30 00:00:00

abstract：:The crystal structure of cholesterol oxidase, a 56kDa flavoenzyme was anisotropically refined to 0.95A resolution. The final crystallographic R-factor and R(free) value is 11.0% and 13.2%, respectively. The quality of the electron density maps has enabled modeling of alternate conformations for 83 residues in the enzy...

journal_title：Journal of molecular biology

authors： Lario PI,Sampson N,Vrielink A

更新日期：2003-03-07 00:00:00