Positions of proteins S14, S18 and S20 in the 30 S ribosomal subunit of Escherichia coli.

abstract：:Hsp90 is a highly conserved molecular chaperone that remodels hundreds of client proteins, many involved in the progression of cancer and other diseases. It functions with the Hsp70 chaperone and numerous cochaperones. The bacterial Hsp90 functions with an Hsp70 chaperone, DnaK, but is independent of Hsp90 cochaperone...

journal_title：Journal of molecular biology

authors： Genest O,Hoskins JR,Kravats AN,Doyle SM,Wickner S

更新日期：2015-12-04 00:00:00

abstract：:Two types of annelid collagens of different sizes were purified, one from acetic acid extracts of the cuticle (length 2.5 microns) and the other, after pepsin digestion, from interstitial spaces of the body wall (0.3 micron). They were obtained from Alvinella pompejana, Alvinella caudata and Paralvinella grasslei coll...

journal_title：Journal of molecular biology

authors： Gaill F,Mann K,Wiedemann H,Engel J,Timpl R

更新日期：1995-02-17 00:00:00

abstract：:Angiotensin I-converting enzyme (ACE), one of the central components of the renin-angiotensin system, is a key therapeutic target for the treatment of hypertension and cardiovascular disorders. Human somatic ACE (sACE) has two homologous domains (N and C). The N- and C-domain catalytic sites have different activities ...

journal_title：Journal of molecular biology

authors： Akif M,Georgiadis D,Mahajan A,Dive V,Sturrock ED,Isaac RE,Acharya KR

更新日期：2010-07-16 00:00:00

abstract：:Proteasomes, major proteolytic sites in eukaryotic cells, play an important part in major histocompatibility class I (MHC I) ligand generation and thus in the regulation of specific immune responses. Their cleavage specificity is of outstanding interest for this process. In order to generalize previously determined cl...

journal_title：Journal of molecular biology

authors： Kuttler C,Nussbaum AK,Dick TP,Rammensee HG,Schild H,Hadeler KP

更新日期：2000-05-05 00:00:00

abstract：:We have examined the equilibrium and kinetic folding properties of two structurally homologous dihydrofolate reductases, Escherichia coli DHFR (EcDHFR) and murine DHFR (MuDHFR), as a function of temperature and ligand concentration. Conformational heterogeneity in native DHFR is well documented, and the results demons...

journal_title：Journal of molecular biology

authors： Clark AC,Frieden C

更新日期：1999-01-29 00:00:00

abstract：:Here, we used data of complete genomes to study comparatively the metabolism of different species. We built phenetic trees based on the enzymatic functions present in different parts of metabolism. Seven broad metabolic classes, comprising a total of 69 metabolic pathways, were comparatively analyzed for 27 fully sequ...

journal_title：Journal of molecular biology

authors： Aguilar D,Aviles FX,Querol E,Sternberg MJ

更新日期：2004-07-09 00:00:00

abstract：:TF1 is a member of the family of type II DNA-binding proteins, which also includes the bacterial HU proteins and the Escherichia coli integration host factor (IHF). Distinctive to TF1, which is encoded by the Bacillus subtilis bacteriophage SPO1, is its preferential binding to DNA in which thymine is replaced by 5-hyd...

journal_title：Journal of molecular biology

authors： Grove A,Galeone A,Mayol L,Geiduschek EP

更新日期：1996-07-12 00:00:00

abstract：:We succeeded in expressing a chimeric myosin that comprises the motor domain of characean algal myosin, (the fastest known motor protein), and the neck and tail domains of Dictyostelium myosin II. Although the chimeric myosin showed an ATPase activity comparable to that of muscle myosin (15 times higher than that of t...

journal_title：Journal of molecular biology

authors： Kashiyama T,Ito K,Yamamoto K

更新日期：2001-08-17 00:00:00

abstract：:A proliferation-inducing ligand (APRIL) is a TNF-like cytokine that stimulates tumor cell growth. Within the TNF ligand superfamily, APRIL is most similar to B-cell activation factor (BAFF) with which it shares 30% sequence identity. APRIL binds the receptors B-cell maturation antigen (BCMA) and TACI with high affinit...

journal_title：Journal of molecular biology

authors： Wallweber HJ,Compaan DM,Starovasnik MA,Hymowitz SG

更新日期：2004-10-15 00:00:00

abstract：:We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA i...

journal_title：Journal of molecular biology

authors： Pohjoismäki JL,Holmes JB,Wood SR,Yang MY,Yasukawa T,Reyes A,Bailey LJ,Cluett TJ,Goffart S,Willcox S,Rigby RE,Jackson AP,Spelbrink JN,Griffith JD,Crouch RJ,Jacobs HT,Holt IJ

更新日期：2010-04-16 00:00:00

abstract：:The 8-17 deoxyribozyme is an in vitro selected enzyme capable of sequence-specific cleavage of RNA. While selected to be a magnesium and zinc-utilizing enzyme, the 8-17 DNAzyme has been shown to utilize lead for its catalysis. Fluorescence-based experiments have indicated that the magnesium- and zinc-utilizing version...

journal_title：Journal of molecular biology

authors： Liu Y,Sen D

更新日期：2010-01-15 00:00:00

abstract：:Catalytic antibodies 6D9 and 9C10, which were induced by immunization with a haptenic transition-state analog (TSA), catalyze the hydrolysis of a nonbioactive chloramphenicol monoester derivative to generate a bioactive chloramphenicol. These antibodies stabilize the transition state to catalyze the hydrolysis reactio...

journal_title：Journal of molecular biology

authors： Oda M,Ito N,Tsumuraya T,Suzuki K,Sakakura M,Fujii I

更新日期：2007-05-25 00:00:00

abstract：:Ubiquitination refers to the covalent addition of ubiquitin (Ub) to substrate proteins or other Ub molecules via the sequential action of three enzymes (E1, E2, and E3). Recent advances in mass spectrometry proteomics have made it possible to identify and quantify Ub linkages in biochemical and cellular systems. We us...

journal_title：Journal of molecular biology

authors： Bosanac I,Phu L,Pan B,Zilberleyb I,Maurer B,Dixit VM,Hymowitz SG,Kirkpatrick DS

更新日期：2011-05-06 00:00:00

abstract：:The aim of the present study was to develop the protein fragment complementation assay (PCA) for the intracellular selection of specific binding molecules from the fully synthetic HuCAL antibody library. Here, we describe the first successful selections of specific antibodies by PCA, and we discuss the opportunities a...

journal_title：Journal of molecular biology

authors： Koch H,Gräfe N,Schiess R,Plückthun A

更新日期：2006-03-24 00:00:00

abstract：:Single crystals of glycosylated recombinant human renin have been obtained using the hanging-drop vapor diffusion method with polyethylene glycol and sodium chloride as coprecipitants. The crystals belong to the cubic space group P2(1)3 with a = 143.0 A and contain two molecules of renin in the asymmetric unit. A self...

journal_title：Journal of molecular biology

authors： Lim LW,Stegeman RA,Leimgruber NK,Gierse JK,Abdel-Meguid SS

更新日期：1989-11-05 00:00:00

abstract：:Bacteriophage with double-stranded, linear DNA genomes package DNA into pre-assembled icosahedral procapsids through a unique vertex. The packaging vertex contains an oligomeric ring of a portal protein that serves as a recognition site for the packaging enzymes, a conduit for DNA translocation, and the site of tail a...

journal_title：Journal of molecular biology

authors： Moore SD,Prevelige PE Jr

更新日期：2002-02-01 00:00:00

abstract：:Protein interactions play a crucial role among the different functions of a cell and are central to our understanding of cellular processes both in health and disease. Here we present Galaxy InteractoMIX (http://galaxy.interactomix.com), a platform composed of 13 different computational tools each addressing specific ...

journal_title：Journal of molecular biology

authors： Mirela-Bota P,Aguirre-Plans J,Meseguer A,Galletti C,Segura J,Planas-Iglesias J,Garcia-Garcia J,Guney E,Oliva B,Fernandez-Fuentes N

更新日期：2020-09-23 00:00:00

abstract：:The restriction enzyme R.BbvCI cleaves duplex DNA within a seven base-pair asymmetric recognition sequence, thus: CCTCAGC/GCTGAGG-->CC--TCAGC/GC--TGAGG. We show that R.BbvCI comprises two different subunits, R(1) and R(2); that each subunit contains a catalytic site for DNA strand hydrolysis; and that these sites act ...

journal_title：Journal of molecular biology

authors： Heiter DF,Lunnen KD,Wilson GG

更新日期：2005-05-06 00:00:00

abstract：:The binding of tryptophan repressor (TrpR) to its operators was examined quantitatively using in vitro and in vivo methods. DNA sequence requirements for 1:1 and tandem 2:1 (TrpR:DNA) binding in various sequence contexts were studied. The results indicate that the optimal half-site sequence for recognition by one heli...

journal_title：Journal of molecular biology

authors： Yang J,Gunasekera A,Lavoie TA,Jin L,Lewis DE,Carey J

更新日期：1996-04-26 00:00:00

abstract：:Functional analysis of hepatitis B virus (HBV) core particles has associated a number of biological roles with the C terminus of the capsid protein. One set of functions require the C terminus to be on the exterior of the capsid, while others place this domain on the interior. According to the crystal structure of the...

journal_title：Journal of molecular biology

authors： Hilmer JK,Zlotnick A,Bothner B

更新日期：2008-01-11 00:00:00

abstract：:A dynamic structure refinement method for X-ray crystallography, referred to as the normal mode refinement, is proposed. The Debye-Waller factor is expanded in terms of the low-frequency normal modes whose amplitudes and eigenvectors are experimentally optimized in the process of the crystallographic refinement. In th...

journal_title：Journal of molecular biology

authors： Kidera A,Go N

更新日期：1992-05-20 00:00:00

abstract：:Collagen fibrils are the principle source of mechanical strength in the mutable dermis of the sea cucumber Cucumaria frondosa. To obtain information about the mechanism by which collagen molecules self-assemble into fibrils, we have isolated single intact fibrils with lengths in the range 14-444 microm. These fibrils ...

journal_title：Journal of molecular biology

authors： Trotter JA,Chapman JA,Kadler KE,Holmes DF

更新日期：1998-12-18 00:00:00

abstract：:The crystal structure of the eubacteria Mycoplasma genitalium ORF MG438 polypeptide, determined by multiple anomalous dispersion and refined at 2.3 A resolution, reveals the organization of S subunits from the Type I restriction and modification system. The structure consists of two globular domains, with about 150 re...

journal_title：Journal of molecular biology

authors： Calisto BM,Pich OQ,Piñol J,Fita I,Querol E,Carpena X

更新日期：2005-08-26 00:00:00

abstract：:The repertoire of naturally occurring protein structures is usually characterised in structural terms at the domain level by their constituent folds. As structure is acknowledged to be an important stepping stone to the understanding of protein function, an appreciation of how individual domain interactions are built ...

journal_title：Journal of molecular biology

authors： Littler SJ,Hubbard SJ

更新日期：2005-02-04 00:00:00

abstract：:Lipoyl-lysine swinging arms are crucial to the reactions catalysed by the 2-oxo acid dehydrogenase multienzyme complexes. A gene encoding a putative lipoate protein ligase (LplA) of Thermoplasma acidophilum was cloned and expressed in Escherichia coli. The recombinant protein, a monomer of molecular mass 29 kDa, was c...

journal_title：Journal of molecular biology

authors： McManus E,Luisi BF,Perham RN

更新日期：2006-02-24 00:00:00

abstract：:Glycoprotein B (gB) of Herpes simplex virus type 1 (HSV-1) plays an essential role in viral entry. A set of more than 100 HpaI (GTTAAC) linker insertion mutations and their derivatives were isolated in plasmids specifying the gB coding and flanking sequences. Mutations including addition, deletion and nonsense mutatio...

journal_title：Journal of molecular biology

authors： Cai WZ,Person S,DebRoy C,Gu BH

更新日期：1988-06-05 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) genome encodes a highly conserved regulatory gene product, Vpr (96 residues, 14kDa), which is incorporated into virions. In the infected cells, Vpr, expressed late in the virus cycle, is believed to function in the early phases of HIV-1 replication, such as nuclear migra...

journal_title：Journal of molecular biology

authors： Morellet N,Bouaziz S,Petitjean P,Roques BP

更新日期：2003-03-14 00:00:00

abstract：:The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosom...

journal_title：Journal of molecular biology

authors： Kono N,Arakawa K,Sato M,Yoshikawa H,Tomita M,Itaya M

更新日期：2014-08-12 00:00:00

abstract：:Loss-of-function mutations in the gene encoding the multifunctional protein, DJ-1, have been implicated in the pathogenesis of early-onset familial Parkinson's disease (PD), suggesting that DJ-1 may act as a neuroprotectant for dopaminergic (DA) neurons. Enhanced autophagy may benefit PD by clearing damaged organelles...

journal_title：Journal of molecular biology

authors： Gao H,Yang W,Qi Z,Lu L,Duan C,Zhao C,Yang H

更新日期：2012-10-19 00:00:00

abstract：:Trichorzianine A 1 is one of the main components of a mixture of related antibiotic peptides (trichorzianines) produced by the fungus Trichoderma harzianum. Good crystals were obtained and allowed X-ray diffraction up to 0.8 A resolution. The space group is orthorhombic, C222(1), Z = 8, a = 64.8 (1) A, b = 9.33 (3) A,...

journal_title：Journal of molecular biology

authors： Bachet B,Brassy C,Morize I,Surcouf E,Mornon JP,Bodo B,Rebuffat S

更新日期：1983-11-05 00:00:00