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Functional expression of a chimeric myosin-containing motor domain of Chara myosin and neck and tail domains of Dictyostelium myosin II.

Abstract：

:We succeeded in expressing a chimeric myosin that comprises the motor domain of characean algal myosin, (the fastest known motor protein), and the neck and tail domains of Dictyostelium myosin II. Although the chimeric myosin showed an ATPase activity comparable to that of muscle myosin (15 times higher than that of the wild-type Dictyostelium myosin II), the motile activity of the chimera was only 1.3 times higher than that of the wild-type. However, this is the first chimeric myosin that showed motile activity faster than at least one of the parent myosins. It was suggested, therefore, that the motor domain of Chara myosin has the potential for performing fast sliding movement.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kashiyama T,Ito K,Yamamoto K

doi

10.1006/jmbi.2001.4883

keywords:

subject

Has Abstract

pub_date

2001-08-17 00:00:00

pages

461-6

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(01)94883-1

journal_volume

311

pub_type

杂志文章

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