Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.

abstract：:beta-1,4-Galactosyltransferase-I (beta4Gal-T1) transfers galactose from UDP-galactose to N-acetylglucosamine (GlcNAc) residues of the branched N-linked oligosaccharide chains of glycoproteins. In an N-linked biantennary oligosaccharide chain, one antenna is attached to the 3-hydroxyl-(1,3-arm), and the other to the 6-...

journal_title：Journal of molecular biology

authors： Ramasamy V,Ramakrishnan B,Boeggeman E,Ratner DM,Seeberger PH,Qasba PK

更新日期：2005-10-14 00:00:00

abstract：:The structural and magnetic properties of the iron-cores of reconstituted horse spleen ferritin and Azotobacter vinelandii bacterioferritin have been investigated by high-resolution transmission electron microscopy, electron diffraction and Mossbauer spectroscopy. The structural properties of native horse spleen ferri...

journal_title：Journal of molecular biology

authors： Mann S,Williams JM,Treffry A,Harrison PM

更新日期：1987-12-05 00:00:00

abstract：:Detailed atomic models of a eubacterial 5 S rRNA (spinach chloroplast 5 S rRNA) and of a eukaryotic 5 S rRNA (somatic and oocyte 5 S rRNA from Xenopus laevis) were built using computer graphic. Both models integrate stereochemical constraints and experimental data on the accessibility of bases and phosphates towards s...

journal_title：Journal of molecular biology

authors： Westhof E,Romby P,Romaniuk PJ,Ebel JP,Ehresmann C,Ehresmann B

更新日期：1989-05-20 00:00:00

abstract：:The structure and activities of the recombination-promoting P22 Erf protein were examined in vitro. Treatment of the protein with elastase produces a stable amino-terminal fragment, consisting of amino acid residues 1 to (approximately) 136. We have purified this fragment, designated fragment B, to apparent homogeneit...

journal_title：Journal of molecular biology

authors： Poteete AR,Sauer RT,Hendrix RW

更新日期：1983-12-25 00:00:00

abstract：:We have performed thin-section electron microscopy on muscle fibers fixed in different mechanically monitored states, in order to identify structural changes in myosin crossbridges associated with force production and maintenance. Tension and stiffness of fibers from glycerinated Lethocerus flight muscle were monitore...

journal_title：Journal of molecular biology

authors： Reedy MC,Reedy MK,Tregear RT

更新日期：1988-11-20 00:00:00

abstract：:Structural analysis of gelsolin domains 4-6 demonstrates that the two highest-affinity calcium ions that activate the molecule are in domains 5 and 6, one in each. An additional calcium site in domain 4 depends on subsequent actin binding and is seen only in the complex. The uncomplexed structure is primed to bind act...

journal_title：Journal of molecular biology

authors： Kolappan S,Gooch JT,Weeds AG,McLaughlin PJ

更新日期：2003-05-23 00:00:00

abstract：:Domain swapping has been shown to be an important mechanism controlling multiprotein assembly and has been suggested recently as a possible mechanism underlying protein aggregation. Understanding oligomerization via domain swapping is therefore of theoretical and practical importance. By using a symmetrized structure-...

journal_title：Journal of molecular biology

authors： Yang S,Levine H,Onuchic JN

更新日期：2005-09-09 00:00:00

abstract：:We developed a novel genetic method for finding functional regions of a protein by the analysis of chimeras formed between homologous proteins. Sets of chimeric genes were made by intramolecular homologous recombination in a linearized plasmid DNA carrying both recA genes of Escherichia coli and Pseudomonas aeruginosa...

journal_title：Journal of molecular biology

authors： Ogawa T,Shinohara A,Ogawa H,Tomizawa J

更新日期：1992-08-05 00:00:00

abstract：:The expression of intracellular antibodies (intrabodies) in eukaryotic cells has provided a powerful tool to manipulate microbial and cellular signaling pathways in a highly precise manner. However, there have been several technical issues that have restricted their more widespread use. In particular, single-chain ant...

journal_title：Journal of molecular biology

authors： Gennari F,Mehta S,Wang Y,St Clair Tallarico A,Palu G,Marasco WA

更新日期：2004-01-02 00:00:00

abstract：:The yeast Vps4 protein (Vps4p) is a member of the AAA protein family (ATPases associated with diverse cellular activities) and a key player in the transport of proteins out of a prevacuolar endosomal compartment. In human cells, we identified two non-allelic orthologous proteins (VPS4-A and VPS4-B) of yeast Vps4p. The...

journal_title：Journal of molecular biology

authors： Scheuring S,Röhricht RA,Schöning-Burkhardt B,Beyer A,Müller S,Abts HF,Köhrer K

更新日期：2001-09-21 00:00:00

abstract：:Native proteins exhibit precise geometric packing of atoms in their hydrophobic interiors. Nonetheless, controversy remains about the role of core side-chain packing in specifying and stabilizing the folded structures of proteins. Here we investigate the role of core packing in determining the conformation and stabili...

journal_title：Journal of molecular biology

authors： Liu J,Cao W,Lu M

更新日期：2002-05-03 00:00:00

abstract：:Understanding gene regulation and its adaptive significance requires not only a detailed knowledge of individual molecular interactions that give rise to changes in gene expression but also an overview of complete genetic networks and the ways in which components within them interact. Increasingly, such studies are be...

journal_title：Journal of molecular biology

authors： Burton NA,Johnson MD,Antczak P,Robinson A,Lund PA

更新日期：2010-09-03 00:00:00

abstract：:Large RNAs often utilize GNRA tetraloops as structural elements to stabilize the overall tertiary fold. These tetraloop-receptor (TR) interactions have a conserved geometry in which the tetraloop docks into the receptor at an angle of ~15° from the helix containing the receptor. Here, we show that the conserved GUAAY ...

journal_title：Journal of molecular biology

authors： Chan RT,Keating KS,Go MC,Toor N

更新日期：2016-12-04 00:00:00

abstract：:Homogeneous detergent-solubilized B873 light-harvesting complexes from a carotenoid-less mutant of the purple non-sulfur bacterium, Rhodospirillum rubrum G9, were reassembled spontaneously into two-dimensional (2D) hexagonal arrays during extensive and controlled dialysis. As the complexes contain only 1 to 2 mol phos...

journal_title：Journal of molecular biology

authors： Ghosh R,Hoenger A,Hardmeyer A,Mihailescu D,Bachofen R,Engel A,Rosenbusch JP

更新日期：1993-05-20 00:00:00

abstract：:The X-ray structure of tryptophanase (Tnase) reveals the interactions responsible for binding of the pyridoxal 5'-phosphate (PLP) and atomic details of the K+ binding site essential for catalysis. The structure of holo Tnase from Proteus vulgaris (space group P2(1)2(1)2(1) with a = 115.0 A, b = 118.2 A, c = 153.7 A) h...

journal_title：Journal of molecular biology

authors： Isupov MN,Antson AA,Dodson EJ,Dodson GG,Dementieva IS,Zakomirdina LN,Wilson KS,Dauter Z,Lebedev AA,Harutyunyan EH

更新日期：1998-02-27 00:00:00

abstract：:gp41 is the protein responsible for the process of membrane fusion that allows primate lentiviruses (HIV and SIV) to enter into their host cells. gp41 ectodomain contains an N-terminal and a C-terminal heptad repeat region (NHR and CHR) connected by an immunodominant loop. In the absence of membranes, the NHR and CHR ...

journal_title：Journal of molecular biology

authors： Peisajovich SG,Blank L,Epand RF,Epand RM,Shai Y

更新日期：2003-03-07 00:00:00

abstract：:The type III secretion system (T3SS) is essential in the pathogenesis of Yersinia pestis, the causative agent of plague. A small protein, LcrG, functions as a chaperone to the tip protein LcrV, and the LcrG-LcrV interaction is important in regulating protein secretion through the T3SS. The atomic structure of the LcrG...

journal_title：Journal of molecular biology

authors： Chaudhury S,de Azevedo Souza C,Plano GV,De Guzman RN

更新日期：2015-09-25 00:00:00

abstract：:Cytochrome c oxidase couples the reduction of dioxygen to proton pumping against an electrochemical gradient. The D-channel, a 25-A-long cavity, provides the principal pathway for the uptake of chemical and pumped protons. A water chain is thought to mediate the relay of protons via a Grotthuss mechanism through the D...

journal_title：Journal of molecular biology

authors： Henry RM,Yu CH,Rodinger T,Pomès R

更新日期：2009-04-17 00:00:00

abstract：:A protein microarray system containing different dilutions of 77 related and non-related proteins was used to show that IgE from subjects allergic to Brazil nut specifically recognise the seed 2S albumin protein (Ber e 1). Further, correctly folded chimaeric 2S albumin proteins containing structural epitope replacemen...

journal_title：Journal of molecular biology

authors： Alcocer MJ,Murtagh GJ,Wilson PB,Progias P,Lin J,Archer DB

更新日期：2004-10-22 00:00:00

abstract：:The use of carbohydrate-mimicking peptides to induce immune responses against surface polysaccharides of pathogenic bacteria offers a novel approach to vaccine development. Factors governing antigenic and immunogenic mimicry, however, are complex and poorly understood. We have addressed this question using the anti-li...

journal_title：Journal of molecular biology

authors： Theillet FX,Saul FA,Vulliez-Le Normand B,Hoos S,Felici F,Weintraub A,Mulard LA,Phalipon A,Delepierre M,Bentley GA

更新日期：2009-05-15 00:00:00

abstract：:The crystal and molecular structure of a triacylglyceride lipase (EC 3.1.1.3) from the fungus Rhizomucor miehei was analyzed using X-ray single crystal diffraction data to 1.9 A resolution. The structure was refined to an R-factor of 0.169 for all available data. The details of the molecular architecture and the cryst...

journal_title：Journal of molecular biology

authors： Derewenda ZS,Derewenda U,Dodson GG

更新日期：1992-10-05 00:00:00

abstract：:The extracellular matrix in tissues such as bone, tendon and cornea contains ordered, parallel arrays of collagen type I fibrils. Cells embedded in these matrices frequently co-align with the collagen fibrils, suggesting that ordered fibrils provide structural or signalling cues for cell polarization. To study mechani...

journal_title：Journal of molecular biology

authors： Friedrichs J,Taubenberger A,Franz CM,Muller DJ

更新日期：2007-09-21 00:00:00

abstract：:Human serum albumin (HSA) is an abundant plasma protein that transports a wide variety of drugs and endogenous compounds. The complex binding capacity of HSA has made it a challenging system to study in detail but in order to develop our understanding of the interactions between ligands for HSA, the locations and rela...

journal_title：Journal of molecular biology

authors： Simard JR,Zunszain PA,Hamilton JA,Curry S

更新日期：2006-08-11 00:00:00

abstract：:Phototaxis in Halobacterium salinarium is the result of an interplay of sensory rhodopsin excitation and adaptation to the stimulus background. Adaptation to orange light, received by sensory rhodopsin I was probed by measuring the behavioral response of cells to a step-like decrease in intensity. Cells were able to a...

journal_title：Journal of molecular biology

authors： Marwan W,Bibikov SI,Montrone M,Oesterhelt D

更新日期：1995-03-03 00:00:00

abstract：:The sigma N class of sigma factors confer upon RNA polymerase the requirement for enhancer-binding activator proteins. The sigma-N (sigma N) protein of Klebsiella pneumoniae was analysed by the assay of purified peptides comprising domains or regions of sigma N defined by proteolysis or by homology alignment, respecti...

journal_title：Journal of molecular biology

authors： Cannon W,Missailidis S,Smith C,Cottier A,Austin S,Moore M,Buck M

更新日期：1995-05-12 00:00:00

abstract：:Recently, progress has been made toward understanding the functional diversity of bacterial microcompartment (MCP) systems, which serve as protein-based metabolic organelles in diverse microbes. New types of MCPs have been identified, including the glycyl-radical propanediol (Grp) MCP. Within these elaborate protein c...

journal_title：Journal of molecular biology

authors： Thompson MC,Wheatley NM,Jorda J,Sawaya MR,Gidaniyan SD,Ahmed H,Yang Z,McCarty KN,Whitelegge JP,Yeates TO

更新日期：2014-09-23 00:00:00

abstract：:The tyrosine residues adjacent to the C termini of the hemoglobin (Hb) subunits, alphaY140 and betaY145, are expected to play important structural roles, because the C termini are the loci of T-state quaternary salt-bridges, and because the tyrosine side-chains bridge the H and F helices via H bonds to the alphaV93 an...

journal_title：Journal of molecular biology

authors： Kneipp J,Balakrishnan G,Chen R,Shen TJ,Sahu SC,Ho NT,Giovannelli JL,Simplaceanu V,Ho C,Spiro TG

更新日期：2006-02-17 00:00:00

abstract：:Protein-protein interactions within the membrane, partially or fully mediated by transmembrane (TM) domains, are involved in many vital cellular processes. Since the unique feature of the membrane environment enables protein-protein assembly that otherwise is not energetically favored in solution, the structural restr...

journal_title：Journal of molecular biology

authors： Sal-Man N,Gerber D,Shai Y

更新日期：2004-11-26 00:00:00

abstract：:Calmodulin (CaM) is a ubiquitous second messenger protein that regulates a variety of structurally and functionally diverse targets in response to changes in Ca(2+) concentration. CaM-dependent protein kinase II (CaMKII) and calcineurin (CaN) are the prominent CaM targets that play an opposing role in many cellular fu...

journal_title：Journal of molecular biology

authors： Yosef E,Politi R,Choi MH,Shifman JM

更新日期：2009-02-06 00:00:00

abstract：:One to four alanines were inserted by site-directed mutagenesis at three different locations within the alpha-helix comprising residues 39 to 50 in bacteriophage T4 lysozyme. All insertion mutants were correctly folded and catalytically active although the insertions led to a thermal destabilization by 1.1 to 4.2 kcal...

journal_title：Journal of molecular biology

authors： Heinz DW,Baase WA,Zhang XJ,Blaber M,Dahlquist FW,Matthews BW

更新日期：1994-02-25 00:00:00