Abstract：
:Recently, progress has been made toward understanding the functional diversity of bacterial microcompartment (MCP) systems, which serve as protein-based metabolic organelles in diverse microbes. New types of MCPs have been identified, including the glycyl-radical propanediol (Grp) MCP. Within these elaborate protein complexes, BMC-domain shell proteins [bacterial microcompartment (in reference to the shell protein domain)] assemble to form a polyhedral barrier that encapsulates the enzymatic contents of the MCP. Interestingly, the Grp MCP contains a number of shell proteins with unusual sequence features. GrpU is one such shell protein whose amino acid sequence is particularly divergent from other members of the BMC-domain superfamily of proteins that effectively defines all MCPs. Expression, purification, and subsequent characterization of the protein showed, unexpectedly, that it binds an iron-sulfur cluster. We determined X-ray crystal structures of two GrpU orthologs, providing the first structural insight into the homohexameric BMC-domain shell proteins of the Grp system. The X-ray structures of GrpU, both obtained in the apo form, combined with spectroscopic analyses and computational modeling, show that the metal cluster resides in the central pore of the BMC shell protein at a position of broken 6-fold symmetry. The result is a structurally polymorphic iron-sulfur cluster binding site that appears to be unique among metalloproteins studied to date.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Thompson MC,Wheatley NM,Jorda J,Sawaya MR,Gidaniyan SD,Ahmed H,Yang Z,McCarty KN,Whitelegge JP,Yeates TOdoi
10.1016/j.jmb.2014.07.018subject
Has Abstractpub_date
2014-09-23 00:00:00pages
3287-3304issue
19eissn
0022-2836issn
1089-8638pii
S0022-2836(14)00366-0journal_volume
426pub_type
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