Zooming in on Transcription Preinitiation.

abstract：:Bile acid molecules are transferred vectorially between basolateral and apical membranes of hepatocytes and enterocytes in the context of the enterohepatic circulation, a process regulating whole body lipid homeostasis. This work addresses the role of the cytosolic lipid binding proteins in the intracellular transfer ...

journal_title：Journal of molecular biology

authors： Pedò M,Löhr F,D'Onofrio M,Assfalg M,Dötsch V,Molinari H

更新日期：2009-12-18 00:00:00

abstract：:Current data suggest that globular domains may form amyloids via different mechanisms. Nevertheless, there are indications that the initiation of the process takes place invariably in the less stable segments of a protein domain. We have studied the sequence and structural conservation of beta(2)-microglobulin that de...

journal_title：Journal of molecular biology

authors： Benyamini H,Gunasekaran K,Wolfson H,Nussinov R

更新日期：2003-06-27 00:00:00

abstract：:Assembly of infectious retroviral particles involves recognition of specific sequences on the viral RNA by the nucleocapsid (NC) domain of the Gag polyprotein, and subsequent stoichiometric binding of the processed NC protein along the entire length of the RNA. NC proteins also act as nucleic acid chaperones. They acc...

journal_title：Journal of molecular biology

authors： Urbaneja MA,Wu M,Casas-Finet JR,Karpel RL

更新日期：2002-05-03 00:00:00

abstract：:The asymmetric unit membrane (AUM) forms numerous plaques covering the apical surface of mammalian urinary bladder epithelium. These plaques contain four major integral membrane proteins called uroplakins Ia, Ib, II and III, which form particles arranged in a well-ordered hexagonal lattice with p6 symmetry and a latti...

journal_title：Journal of molecular biology

authors： Walz T,Häner M,Wu XR,Henn C,Engel A,Sun TT,Aebi U

更新日期：1995-05-19 00:00:00

abstract：:Collagen fibrils are the principle source of mechanical strength in the mutable dermis of the sea cucumber Cucumaria frondosa. To obtain information about the mechanism by which collagen molecules self-assemble into fibrils, we have isolated single intact fibrils with lengths in the range 14-444 microm. These fibrils ...

journal_title：Journal of molecular biology

authors： Trotter JA,Chapman JA,Kadler KE,Holmes DF

更新日期：1998-12-18 00:00:00

abstract：:Alzheimer's disease (AD) and Parkinson's disease (PD) are the two most prevalent neurodegenerative diseases of the central nervous system. These two diseases share a common feature in that a normally soluble peptide (amyloid-beta) or protein (alpha-synuclein) aggregates into an ordered fibrillar structure. As well as ...

journal_title：Journal of molecular biology

authors： Duce JA,Smith DP,Blake RE,Crouch PJ,Li QX,Masters CL,Trounce IA

更新日期：2006-08-18 00:00:00

abstract：:Three crystal forms of canine myeloperoxidase are described. An orthorhombic form in space group P2(1)2(1)2(1) has unit cell dimensions: a = 108.3 A (1 A = 0.1 nm) b = 205.9 A and c = 139.9 A. A trigonal form in space group P3(1)21 or P3(2)21 has unit cell dimensions: a = b = 138.9 A and c = 145.2 A. A monoclinic form...

journal_title：Journal of molecular biology

authors： Fenna RE

更新日期：1987-08-20 00:00:00

abstract：:Here we determined NMR solution structures of two mutants of bovine pancreatic trypsin inhibitor (BPTI) to reveal structural reasons of their decreased thermodynamic stability. A point mutation, A16V, in the solvent-exposed loop destabilizes the protein by 20 degrees C, in contrast to marginal destabilization observed...

journal_title：Journal of molecular biology

authors： Cierpicki T,Otlewski J

更新日期：2002-08-23 00:00:00

abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

journal_title：Journal of molecular biology

authors： Wei Z,Xue Y,Xu H,Gong W

更新日期：2006-05-26 00:00:00

abstract：:The multifunctional AAA-ATPase p97 is one of the most abundant and conserved proteins in eukaryotic cells. The p97/Npl4/Ufd1 complex dislocates proteins that fail the protein quality control in the endoplasmic reticulum to the cytosol where they are subject to degradation by the ubiquitin/proteasome system. Substrate ...

journal_title：Journal of molecular biology

authors： Beskow A,Grimberg KB,Bott LC,Salomons FA,Dantuma NP,Young P

更新日期：2009-12-11 00:00:00

abstract：:Cytoplasmic Ca2+ signals are highly regulated by various ion transporters, including the inositol 1,4,5-trisphosphate (IP(3)) receptor (IP(3)R), which functions as a Ca2+ release channel on the endoplasmic reticulum membrane. Crystal structures of the two N-terminal regulatory regions from type 1 IP(3)R have been repo...

journal_title：Journal of molecular biology

authors： Chan J,Whitten AE,Jeffries CM,Bosanac I,Mal TK,Ito J,Porumb H,Michikawa T,Mikoshiba K,Trewhella J,Ikura M

更新日期：2007-11-09 00:00:00

abstract：:Azaleucine is a naturally occurring amino acid antibiotic that can be incorporated into proteins by mimicking leucine. In spite of its highly toxic character, the contrast between the hydrophobic side-chain of leucine and the basic side-chain of azaleucine suggested a mechanism for rescuing certain mutants. We constru...

journal_title：Journal of molecular biology

authors： Lemeignan B,Sonigo P,Marlière P

更新日期：1993-05-20 00:00:00

abstract：:A 26 Å resolution map of the structure of human low-density lipoprotein (LDL) was obtained from electron cryomicroscopy and single-particle image reconstruction. The structure showed a discoidal-shaped LDL particle with high-density regions mainly distributed at the edge of the particle and low-density regions at the ...

journal_title：Journal of molecular biology

authors： Liu Y,Atkinson D

更新日期：2011-01-07 00:00:00

abstract：:Dimers of the Rel/NFkappaB transcription factor family form with differential stabilities through the combinatorial association of five polypeptides: p50, p52, p65, cRel, and RelB. Here, we have characterized the nature of the monomer-dimer equilibrium of the p50 homodimer. Sedimentation equilibrium studies show that ...

journal_title：Journal of molecular biology

authors： Sengchanthalangsy LL,Datta S,Huang DB,Anderson E,Braswell EH,Ghosh G

更新日期：1999-06-18 00:00:00

abstract：:The crystal structures of three vancomycin complexes with two vancomycin-sensitive cell-wall precursor analogs (diacetyl-Lys-D-Ala-D-Ala and acetyl-D-Ala-D-Ala) and a vancomycin-resistant cell-wall precursor analog (diacetyl-Lys-D-Ala-D-lactate) were determined at atomic resolutions of 1.80 A, 1.07 A, and 0.93 A, resp...

journal_title：Journal of molecular biology

authors： Nitanai Y,Kikuchi T,Kakoi K,Hanamaki S,Fujisawa I,Aoki K

更新日期：2009-02-06 00:00:00

abstract：:The soluble domain of the self-assembly vaccinia virus envelope protein A27L, sA27L-aa, consists of a flexible extended coil at the N terminus and a rigid hydrophobic coiled-coil region at the C terminus. In the former, a basic strip of 12 residues is responsible for binding to cell-surface heparan sulfates. Although ...

journal_title：Journal of molecular biology

authors： Ho Y,Hsiao JC,Yang MH,Chung CS,Peng YC,Lin TH,Chang W,Tzou DL

更新日期：2005-06-24 00:00:00

abstract：:The structure of goose egg-white lysozyme (GEWL) has been refined to an R-value of 15.9% at 1.6 A resolution. Details of the structure determination, the refinement and the structure itself are presented. The structure of a complex of the enzyme with the trisaccharide of N-acetyl glucosamine has also been determined a...

journal_title：Journal of molecular biology

authors： Weaver LH,Grütter MG,Matthews BW

更新日期：1995-01-06 00:00:00

abstract：:We have developed a procedure to predict the peptide binding specificity of an SH3 domain from its sequence. The procedure utilizes information extracted from position-specific contacts derived from six SH3/peptide or SH3/protein complexes of known structure. The framework of SH3/peptide contacts defined on the struct...

journal_title：Journal of molecular biology

authors： Brannetti B,Via A,Cestra G,Cesareni G,Helmer-Citterich M

更新日期：2000-04-28 00:00:00

abstract：:The minichromosome maintenance (MCM) proteins are essential for replication initiation and elongation in eukarya and archaea. There are six MCM proteins in eukaryotes, and MCM complexes are believed to unwind DNA during chromosomal DNA replication. However, the mechanism and structure of the MCM complexes are not know...

journal_title：Journal of molecular biology

authors： Chen YJ,Yu X,Kasiviswanathan R,Shin JH,Kelman Z,Egelman EH

更新日期：2005-02-18 00:00:00

abstract：:The structure of the yeast DNA-dependent RNA polymerase I (RNA Pol I), prepared by cryo-negative staining, was studied by electron microscopy. A structural model of the enzyme at a resolution of 1.8 nm was determined from the analysis of isolated molecules and showed an excellent fit with the atomic structure of the R...

journal_title：Journal of molecular biology

authors： De Carlo S,Carles C,Riva M,Schultz P

更新日期：2003-06-20 00:00:00

abstract：:Following acetate, propionate is the second most abundant low molecular mass carbon compound found in soil. Many microorganisms, including most, if not all fungi, as well as several aerobic bacteria, such as Escherichia coli and Salmonella enterica oxidize propionate via the methylcitrate cycle. The enzyme 2-methyliso...

journal_title：Journal of molecular biology

authors： Grimm C,Evers A,Brock M,Maerker C,Klebe G,Buckel W,Reuter K

更新日期：2003-05-02 00:00:00

abstract：:Voltage-dependent H(+) (Hv) channels mediate proton conduction into and out of cells under the control of membrane voltage. Hv channels are unusual compared to voltage-dependent K(+), Na(+), and Ca(2+) channels in that Hv channel genes encode a voltage sensor domain (VSD) without a pore domain. The H(+) currents obser...

journal_title：Journal of molecular biology

authors： Lee SY,Letts JA,MacKinnon R

更新日期：2009-04-17 00:00:00

abstract：:Peptide deformylases (PDFs) are essential enzymes of the N-terminal protein processing pathway of eubacteria. The recent discovery of two types of PDFs in higher plants, PDF1A and PDF1B, and the detection of PDF1A in humans, have raised questions concerning the importance of deformylation in eukaryotes. Here, we have ...

journal_title：Journal of molecular biology

authors： Serero A,Giglione C,Meinnel T

更新日期：2001-12-07 00:00:00

abstract：:Harold Erickson has recently provided a useful analysis of helical structures having one class versus two classes of intersubunit bonds. His analysis is based upon an assumption that the subunits themselves are essentially unchanged upon bond formation (polymerization). He shows that such a structure having two classe...

journal_title：Journal of molecular biology

authors： Egelman EH,DeRosier DJ

更新日期：1991-02-05 00:00:00

abstract：:The pathological Aβ aggregates associated with Alzheimer's disease follow a nucleation-dependent path of formation. A nucleus represents an oligomeric assembly of Aβ peptides that acts as a template for subsequent incorporation of monomers to form a fibrillar structure. Nuclei can form de novo or via surface-catalyzed...

journal_title：Journal of molecular biology

authors： Nilsson L,Pamrén A,Islam T,Brännström K,Golchin SA,Pettersson N,Iakovleva I,Sandblad L,Gharibyan AL,Olofsson A

更新日期：2018-08-17 00:00:00

abstract：:Selective autophagy requires the specific segregation of targeted proteins into autophagosomes. The selectivity is mediated by autophagy receptors, such as p62 and NBR1, which can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. Recogni...

journal_title：Journal of molecular biology

authors： Rozenknop A,Rogov VV,Rogova NY,Löhr F,Güntert P,Dikic I,Dötsch V

更新日期：2011-07-15 00:00:00

abstract：:The thermal melting of a dicyclic 29-residue peptide, having helix-stabilizing side-chain to side-chain covalent links at each terminal, has been studied by circular dichroism spectropolarimetry (CD) and differential scanning calorimetry (DSC). The CD spectra for this dicyclic peptide indicate that it is monomeric, al...

journal_title：Journal of molecular biology

authors： Taylor JW,Greenfield NJ,Wu B,Privalov PL

更新日期：1999-08-27 00:00:00

abstract：:RIG-I-like receptors (RLRs) are nucleic acid sensors that activate antiviral innate immune response. These molecules recognize diverse non-self RNA substrates and are antagonized by several viral inhibitors. We performed an evolutionary analysis of RLR genes (RIG-I, MDA5, and LGP2) in mammals. Results indicated that p...

journal_title：Journal of molecular biology

authors： Cagliani R,Forni D,Tresoldi C,Pozzoli U,Filippi G,Rainone V,De Gioia L,Clerici M,Sironi M

更新日期：2014-03-20 00:00:00

abstract：:Two crystal habits, one rod shaped and the other square prismatic, of the Fab fragment of a monoclonal anti-phenylalanine hydroxylase antibody have been grown using the method of vapour phase diffusion against polyethylene glycol 6000. The square prisms diffract to better than 2.8 A, belong to the space group P1 and h...

journal_title：Journal of molecular biology

authors： Guddat LW,Cowan SW,Machin KJ,Isaacs NW,Cotton RG

更新日期：1985-11-20 00:00:00

abstract：:The thermostable tailspike endorhamnosidase of bacteriophage P22 has been investigated by laser Raman spectroscopy to determine the protein's secondary structure and the basis of its thermostability. The conformation of the native tailspike, determined by Raman amide I and amide III band analyses, is 52 to 61% beta-sh...

journal_title：Journal of molecular biology

authors： Sargent D,Benevides JM,Yu MH,King J,Thomas GJ Jr

更新日期：1988-02-05 00:00:00