Crystallization and preliminary crystallographic data of the Fab fragment of an anti-phenylalanine hydroxylase monoclonal antibody.

abstract：:EcoP15I is a Type III restriction endonuclease requiring the interaction with two inversely oriented 5'-CAGCAG recognition sites for efficient DNA cleavage. Diverse models have been developed to explain how enzyme complexes bound to both sites move toward each other, DNA translocation, DNA looping and simple diffusion...

journal_title：Journal of molecular biology

authors： Möncke-Buchner E,Rothenberg M,Reich S,Wagenführ K,Matsumura H,Terauchi R,Krüger DH,Reuter M

更新日期：2009-04-17 00:00:00

abstract：:The pathological Aβ aggregates associated with Alzheimer's disease follow a nucleation-dependent path of formation. A nucleus represents an oligomeric assembly of Aβ peptides that acts as a template for subsequent incorporation of monomers to form a fibrillar structure. Nuclei can form de novo or via surface-catalyzed...

journal_title：Journal of molecular biology

authors： Nilsson L,Pamrén A,Islam T,Brännström K,Golchin SA,Pettersson N,Iakovleva I,Sandblad L,Gharibyan AL,Olofsson A

更新日期：2018-08-17 00:00:00

abstract：:Ground-state dynamics in RNA is a critical precursor for structural adaptation observed ubiquitously in protein-RNA recognition. A tertiary conformational analysis of the stem-loop structural element in the transactivation response element (TAR) from human immunodeficiency virus type 1 (HIV-I) RNA is presented using r...

journal_title：Journal of molecular biology

authors： Al-Hashimi HM,Gosser Y,Gorin A,Hu W,Majumdar A,Patel DJ

更新日期：2002-01-11 00:00:00

abstract：:The Gasdermin (GSDM) family consists of Gasdermin A (GSDMA), Gasdermin B (GSDMB), Gasdermin C (GSDMC), Gasdermin D (GSDMD), Gasdermin E (GSDME) and Pejvakin (PJVK). GSDMD is activated by inflammasome-associated inflammatory caspases. Cleavage of GSDMD by human or mouse caspase-1, human caspase-4, human caspase-5, and ...

journal_title：Journal of molecular biology

authors： Feng S,Fox D,Man SM

更新日期：2018-09-14 00:00:00

abstract：:Hepatocyte nuclear factor 1 (HNF1) is a dimeric homeoprotein expressed in hepatocytes and in a few other epithelial cells where it helps regulate the expression of a specific subset of genes. In an attempt to identify novel target genes for HNF1 and to assess the distribution of its target sites within the vertebrate ...

journal_title：Journal of molecular biology

authors： Tronche F,Ringeisen F,Blumenfeld M,Yaniv M,Pontoglio M

更新日期：1997-02-21 00:00:00

abstract：:The cap-binding protein eIF4E is the first in a chain of translation initiation factors that recruit 40S ribosomal subunits to the 5' end of eukaryotic mRNA. During cap-dependent translation, this protein binds to the 5'-terminal m(7)Gppp cap of the mRNA, as well as to the adaptor protein eIF4G. The latter then intera...

journal_title：Journal of molecular biology

authors： von der Haar T,Oku Y,Ptushkina M,Moerke N,Wagner G,Gross JD,McCarthy JE

更新日期：2006-03-03 00:00:00

abstract：:RIG-I-like receptors (RLRs) are nucleic acid sensors that activate antiviral innate immune response. These molecules recognize diverse non-self RNA substrates and are antagonized by several viral inhibitors. We performed an evolutionary analysis of RLR genes (RIG-I, MDA5, and LGP2) in mammals. Results indicated that p...

journal_title：Journal of molecular biology

authors： Cagliani R,Forni D,Tresoldi C,Pozzoli U,Filippi G,Rainone V,De Gioia L,Clerici M,Sironi M

更新日期：2014-03-20 00:00:00

abstract：:The cleavage site of the T4 prohead protease in gene product 68 of bacteriophage T4 has been determined by direct protein sequencing. It is located close to the carboxy-terminal end of a predicted alpha-helix in the sequence Asn-Val-Glu-Ala between the Glu and Ala residues. Secondary structure seems to be more importa...

journal_title：Journal of molecular biology

authors： Keller B,Kellenberger E,Bickle TA,Tsugita A

更新日期：1985-12-05 00:00:00

abstract：:The Escherichia coli lac operon provides a classic paradigm for understanding regulation of gene transcription. It is now appreciated that lac promoter repression involves cooperative binding of the bidentate lac repressor tetramer to pairs of lac operators via DNA looping. We have adapted components of this system to...

journal_title：Journal of molecular biology

authors： Becker NA,Kahn JD,Maher LJ 3rd

更新日期：2005-06-17 00:00:00

abstract：:Previous kinetic studies demonstrated that nucleotide-derived conformational changes regulate function in the COOH-terminal Src kinase. We have employed enhanced methods of hydrogen-deuterium exchange-mass spectrometry (DXMS) to probe conformational changes on CSK in the absence and presence of nucleotides and thereby...

journal_title：Journal of molecular biology

authors： Hamuro Y,Wong L,Shaffer J,Kim JS,Stranz DD,Jennings PA,Woods VL Jr,Adams JA

更新日期：2002-11-08 00:00:00

abstract：:The replication of premutagenic DNA lesions generates mutant progeny in patterns that distinguish lesions that rarely produce a mutation per DNA replication from those that frequently do so. The quantitative aspects of this distinction were tested in studies of heat-mutagenized bacteriophage T4. Previous T4 studies ha...

journal_title：Journal of molecular biology

authors： Ripley LS

更新日期：1988-07-05 00:00:00

abstract：:To determine the conformational requirements for antibody recognition and extent of flexibility within a protein epitope, a chimeric influenza A virus neuraminidase (NA) has been constructed in which five discontinuous polypeptide segments from a subtype N9 NA, which comprise the monoclonal antibody NC41 epitope, have...

journal_title：Journal of molecular biology

authors： Nuss JM,Air GM

更新日期：1994-01-14 00:00:00

abstract：:The structure of mucor pusillus pepsin (EC 3.4.23.6), the aspartic proteinase from Mucor pusillus, has been refined to a crystallographic R-factor of 16.2% at 2.0 A resolution. The positions of 2638 protein atoms, 221 solvent atoms and a sulphate ion have been determined with an estimated root-mean-square (r.m.s.) err...

journal_title：Journal of molecular biology

authors： Newman M,Watson F,Roychowdhury P,Jones H,Badasso M,Cleasby A,Wood SP,Tickle IJ,Blundell TL

更新日期：1993-03-05 00:00:00

abstract：:The crystal structures of the nucleotide-empty (A(E)), 5'-adenylyl-beta,gamma-imidodiphosphate (A(PNP))-bound, and ADP (A(DP))-bound forms of the catalytic A subunit of the energy producer A(1)A(O) ATP synthase from Pyrococcus horikoshii OT3 have been solved at 2.47 A and 2.4 A resolutions. The structures provide nove...

journal_title：Journal of molecular biology

authors： Kumar A,Manimekalai MS,Balakrishna AM,Jeyakanthan J,Grüber G

更新日期：2010-02-19 00:00:00

abstract：:Crystal structures of Bacillus stearothermophilus TRS40 neopullulanase and its complexes with panose, maltotetraose and isopanose were determined at resolutions of 1.9, 2.4, 2.8 and 3.2A, respectively. Since the latter two carbohydrates are substrates of this enzyme, a deactivated mutant at the catalytic residue Glu35...

journal_title：Journal of molecular biology

authors： Hondoh H,Kuriki T,Matsuura Y

更新日期：2003-02-07 00:00:00

abstract：:The development of electrostatic interactions during the folding of the N-terminal domain of the ribosomal protein L9 (NTL9) is investigated by pH-dependent rate equilibrium free energy relationships. We show that Asp8, among six acidic residues, is involved in non-native, electrostatic interactions with K12 in the tr...

journal_title：Journal of molecular biology

authors： Cho JH,Raleigh DP

更新日期：2006-06-23 00:00:00

abstract：:We have developed a formalism and a computational method for analyzing the potential functional consequences of non-synonymous single nucleotide polymorphisms. Our approach uses a structural model and phylogenetic information to derive a selection of structure and sequence-based features serving as indicators of an am...

journal_title：Journal of molecular biology

authors： Chasman D,Adams RM

更新日期：2001-03-23 00:00:00

abstract：:Archaebacterial respiratory chains are poorly understood at the molecular level. We have cloned and sequenced a cluster of five new genes from the archaebacterium Sulfolobus acidocaldarius, four of them coding for redox proteins: a Rieske iron-sulphur protein, a cytochrome b, a subunit II of cytochrome oxidase and a b...

journal_title：Journal of molecular biology

authors： Castresana J,Lübben M,Saraste M

更新日期：1995-07-07 00:00:00

abstract：:Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C222(1) form was obtained in citrate at pH 5.3 and an orthorhombic I222 form...

journal_title：Journal of molecular biology

authors： Weaver TM,Levitt DG,Banaszak LJ

更新日期：1993-05-05 00:00:00

abstract：:Small, fast-growing bacteria make ideal subjects for genetic and quantitative analysis alike. Long the darling of theoreticians, efforts to understand the relationship between cell growth and cell cycle progression in bacterial systems have been propelled by modelers and empiricist in equal measure. Taking a historica...

journal_title：Journal of molecular biology

authors： Levin PA,Taheri-Araghi S

更新日期：2019-05-17 00:00:00

abstract：:Peptide deformylase (PDF) has received considerable attention during the last few years as a potential target for a new type of antibiotics. It is an essential enzyme in eubacteria for the removal of the formyl group from the N terminus of the nascent polypeptide chain. We have solved the X-ray structures of four memb...

journal_title：Journal of molecular biology

authors： Kreusch A,Spraggon G,Lee CC,Klock H,McMullan D,Ng K,Shin T,Vincent J,Warner I,Ericson C,Lesley SA

更新日期：2003-07-04 00:00:00

abstract：:Phospholamban physically interacts with the sarcoplasmic reticulum calcium pump (SERCA) and regulates contractility of the heart in response to adrenergic stimuli. We studied this interaction using electron microscopy of 2D crystals of SERCA in complex with phospholamban. In earlier studies, phospholamban oligomers we...

journal_title：Journal of molecular biology

authors： Glaves JP,Trieber CA,Ceholski DK,Stokes DL,Young HS

更新日期：2011-01-21 00:00:00

abstract：:The origin of metal ion selectivity by members of the SmtB/ArsR family of bacterial metal-sensing transcriptional repressors and the mechanism of negative allosteric regulation of DNA binding is poorly understood. Here, we report that two homologous zinc sensors, Staphylococcus aureus CzrA and cyanobacterial SmtB, are...

journal_title：Journal of molecular biology

authors： Eicken C,Pennella MA,Chen X,Koshlap KM,VanZile ML,Sacchettini JC,Giedroc DP

更新日期：2003-10-31 00:00:00

abstract：:The interactions between the complement receptor type 2 (CR2) and the C3 complement fragments C3d, C3dg, and iC3b are essential for the initiation of a normal immune response. A crystal-derived structure of the two N-terminal short consensus repeat (SCR1-2) domains of CR2 in complex with C3d has previously been elucid...

journal_title：Journal of molecular biology

authors： Shaw CD,Storek MJ,Young KA,Kovacs JM,Thurman JM,Holers VM,Hannan JP

更新日期：2010-12-10 00:00:00

abstract：:We have probed the structure of the human mitochondrial DNA helicase, an enzyme that uses the energy of nucleotide hydrolysis to unwind duplex DNA during mitochondrial DNA replication. This novel helicase shares substantial amino acid sequence and functional similarities with the bacteriophage T7 primase-helicase. We ...

journal_title：Journal of molecular biology

authors： Ziebarth TD,Farr CL,Kaguni LS

更新日期：2007-04-13 00:00:00

abstract：:The monoclonal anti-tumor antibody BR96 binds a tetrasaccharide, Lewis y (Le(y)), in vitro and recognizes a Le(y)-bearing or Le(y)-related tumor-associated antigen in vivo. The Fab of the murine monoclonal antibody, mBR96 (IgG3, kappa), and the Fab' of its human chimera, cBR96 (IgG1, kappa), and their complexes with L...

journal_title：Journal of molecular biology

authors： Chang CY,Jeffrey PD,Bajorath J,Hellström I,Hellström KE,Sheriff S

更新日期：1994-01-07 00:00:00

abstract：:The MA (matrix) domain of the retroviral Gag polyprotein plays several critical roles during virus assembly. Although best known for targeting the Gag polyprotein to the inner leaflet of the plasma membrane for virus budding, recent studies have revealed that MA also contributes to selective packaging of the genomic R...

journal_title：Journal of molecular biology

authors： Parent LJ,Gudleski N

更新日期：2011-07-22 00:00:00

abstract：:The superfamily 1 bacterial helicase PcrA has a role in the replication of certain plasmids, acting with the initiator protein (RepD) that binds to and nicks the double-stranded origin of replication. PcrA also translocates single-stranded DNA with discrete steps of one base per ATP hydrolyzed. Individual rate constan...

journal_title：Journal of molecular biology

authors： Toseland CP,Martinez-Senac MM,Slatter AF,Webb MR

更新日期：2009-10-02 00:00:00

abstract：:Recruitment of signaling molecules to the cytoplasmic domains of the CD3 subunits of the T-cell receptor (TCR) is crucial for early T-cell activation. These transient associations either do or do not require tyrosine phosphorylation of CD3 immune tyrosine activation motifs (ITAMs). Here we show that the non-ITAM-requi...

journal_title：Journal of molecular biology

authors： Takeuchi K,Yang H,Ng E,Park SY,Sun ZY,Reinherz EL,Wagner G

更新日期：2008-07-18 00:00:00

abstract：:The projected structure of the vanadate-induced crystalline aggregates of Ca2+-ATPase molecules in isolated sarcoplasmic reticulum membranes has been determined. The molecules form tubular crystals with an oblique surface lattice having cell dimensions a = 65.9 A, b = 114.4 A and gamma = 77.9 degrees. The space group ...

journal_title：Journal of molecular biology

authors： Taylor K,Dux L,Martonosi A

更新日期：1984-03-25 00:00:00