当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > The refined structures of goose lysozyme and its complex with a bound trisaccharide show that the "goose-type" lysozymes lack a catalytic aspartate residue.

The refined structures of goose lysozyme and its complex with a bound trisaccharide show that the "goose-type" lysozymes lack a catalytic aspartate residue.

Abstract：

:The structure of goose egg-white lysozyme (GEWL) has been refined to an R-value of 15.9% at 1.6 A resolution. Details of the structure determination, the refinement and the structure itself are presented. The structure of a complex of the enzyme with the trisaccharide of N-acetyl glucosamine has also been determined and refined at 1.6 A resolution. The trisaccharide occupies sites analogous to the B, C and D subsites of chicken (HEWL) and phage T4 (T4L) lysozymes. All three lysozymes (GEWL, HEWL and T4L) display the same characteristic set of bridging hydrogen bonds between backbone atoms of the protein and the 2-acetamido group of the saccharide in subsite C. Glu73 of GEWL is seen to correspond closely to Glu35 of HEWL (and to Glu11 of T4L) and supports the established view that this group is critically involved in the catalytic mechanism. There is, however, no obvious residue in goose lysozyme that is a counterpart of Asp52 of chicken lysozyme (or of Asp20 in T4L), suggesting that a second acidic residue is not essential for the catalytic activity of goose lysozyme, and may not be required for the activity of other lysozymes.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Weaver LH,Grütter MG,Matthews BW

doi

10.1016/s0022-2836(95)80038-7

subject

Has Abstract

pub_date

1995-01-06 00:00:00

pages

54-68

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(95)80038-7

journal_volume

245

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • The Structure of Physarum polycephalum hemagglutinin I suggests a minimal carbohydrate recognition domain of legume lectin fold.

    abstract：:Physarum polycephalum hemagglutinin I (HA1) is a 104-residue protein that is secreted to extracellular space. The crystal structure of HA1 has a β-sandwich fold found among lectin structures, such as legume lectins and galectins. Interestingly, the β-sandwich of HA1 lacks a jelly roll motif and is essentially composed...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.11.024

    authors： Kouno T,Watanabe N,Sakai N,Nakamura T,Nabeshima Y,Morita M,Mizuguchi M,Aizawa T,Demura M,Imanaka T,Tanaka I,Kawano K

    更新日期：2011-01-14 00:00:00

  • In vitro packaging of DNA of the Bacillus subtilis bacteriophage SPP1.

    abstract：:In vitro packaging of bacteriophage SPP1 DNA into procapsids is described and the requirements of this process were determined. Combination of proheads with an extract supplying terminase, DNA and phage tails yielded up to 10(7 )viable phages per milliliter of in vitro reaction under optimized conditions. The presence...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3449

    authors： Dröge A,Tavares P

    更新日期：2000-02-11 00:00:00

  • Molecular determinants of the yeast Arc1p-aminoacyl-tRNA synthetase complex assembly.

    abstract：:Eukaryotic aminoacyl-tRNA synthetases are usually organized into high-molecular-weight complexes, the structure and function of which are poorly understood. We have previously described a yeast complex containing two aminoacyl-tRNA synthetases, methionyl-tRNA synthetase and glutamyl-tRNA synthetase, and one noncatalyt...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.10.010

    authors： Karanasios E,Simader H,Panayotou G,Suck D,Simos G

    更新日期：2007-12-07 00:00:00

  • Conformational isomers of a class II MHC-peptide complex in solution.

    abstract：:A number of kinetic measurements of peptide dissociation from class II MHC-peptide complexes provide compelling evidence for the existence of conformational isomers in solution. There is evidence that T-lymphocytes can distinguish such isomers. However, virtually nothing is known about the structure of these isomers. ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2463

    authors： Schmitt L,Boniface JJ,Davis MM,McConnell HM

    更新日期：1999-02-12 00:00:00

  • Folding energetics of ligand binding proteins. I. Theoretical model.

    abstract：:Heat capacity curves as obtained from differential scanning calorimetry are an outstanding source for molecular information on protein folding and ligand-binding energetics. However, deconvolution of C(p) data of proteins in the presence of ligands can be compromised by indeterminacies concerning the correct choice of...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4359

    authors： Rösgen J,Hinz HJ

    更新日期：2001-03-02 00:00:00

  • Effects of the nucleoid protein HU on the structure, flexibility, and ring-closure properties of DNA deduced from Monte Carlo simulations.

    abstract：:The histone-like HU (heat unstable) protein plays a key role in the organization and regulation of the Escherichia coli genome. The nonspecific nature of HU binding to DNA complicates analysis of the mechanism by which the protein contributes to the looping of DNA. Conventional models of the looping of HU-bound duplex...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.05.088

    authors： Czapla L,Swigon D,Olson WK

    更新日期：2008-10-03 00:00:00

  • Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase.

    abstract：:Crystal structures of Bacillus stearothermophilus TRS40 neopullulanase and its complexes with panose, maltotetraose and isopanose were determined at resolutions of 1.9, 2.4, 2.8 and 3.2A, respectively. Since the latter two carbohydrates are substrates of this enzyme, a deactivated mutant at the catalytic residue Glu35...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01402-x

    authors： Hondoh H,Kuriki T,Matsuura Y

    更新日期：2003-02-07 00:00:00

  • The crystal structure of Escherichia coli class II fructose-1, 6-bisphosphate aldolase in complex with phosphoglycolohydroxamate reveals details of mechanism and specificity.

    abstract：:The structure of a class II fructose-1,6-bisphosphate aldolase in complex with the substrate analogue and inhibitor phosphoglycolohydroxamate (PGH) has been determined using X-ray diffraction terms to a resolution of 2.0 A (1 A=0.1 nm). The crystals are trigonal, space group P3121 with a=b=78.24 A, c=289.69 A. The asy...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2609

    authors： Hall DR,Leonard GA,Reed CD,Watt CI,Berry A,Hunter WN

    更新日期：1999-03-26 00:00:00

  • Yeast RNase III as a key processing enzyme in small nucleolar RNAs metabolism.

    abstract：:The variety of biogenesis pathways for small nucleolar RNAs (snoRNAs) reflects the diversity of their genomic organization. We have searched for yeast snoRNAs which are affected by the depletion of the yeast ortholog of bacterial RNase III, Rnt1. In a yeast strain inactivated for RNT1, almost half of the snoRNAs teste...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2237

    authors： Chanfreau G,Legrain P,Jacquier A

    更新日期：1998-12-11 00:00:00

  • The Third Coiled Coil Domain of Atg11 Is Required for Shaping Mitophagy Initiation Sites.

    abstract：:Selective autophagy is the capture of specific cytosolic contents in double-membrane vesicles that subsequently fuse with the vacuole or lysosome, thereby delivering cargo for degradation. Selective autophagy receptors (SARs) mark the cargo for degradation and, in yeast, recruit Atg11, the scaffolding protein for sele...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.08.025

    authors： Margolis HK,Katzenell S,Leary KA,Ragusa MJ

    更新日期：2020-10-02 00:00:00

  • A direct estimation of the context effect on the efficiency of termination.

    abstract：:An in vitro assay in which terminating Escherichia coli ribosomes with different stop signals in the A-site compete for a limited amount of a release factor (RF1 or RF2) has been used to estimate the relative termination efficiencies at stop codons with different adjacent downstream nucleotides. The assay allows direc...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2220

    authors： Pavlov MY,Freistroffer DV,Dincbas V,MacDougall J,Buckingham RH,Ehrenberg M

    更新日期：1998-12-04 00:00:00

  • Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by yeast surface display: molecular convergence with single-domain camelid and shark antibodies.

    abstract：:The 10th human fibronectin type III domain ((10)Fn3) is one of several protein scaffolds used to design and select families of proteins that bind with high affinity and specificity to macromolecular targets. To date, the highest affinity (10)Fn3 variants have been selected by mRNA display of libraries generated by ran...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.02.029

    authors： Lipovsek D,Lippow SM,Hackel BJ,Gregson MW,Cheng P,Kapila A,Wittrup KD

    更新日期：2007-05-11 00:00:00

  • Structural studies of MFE-1: the 1.9 A crystal structure of the dehydrogenase part of rat peroxisomal MFE-1.

    abstract：:The 1.9 A structure of the C-terminal dehydrogenase part of the rat peroxisomal monomeric multifunctional enzyme type 1 (MFE-1) has been determined. In this construct (residues 260-722 and referred to as MFE1-DH) the N-terminal hydratase part of MFE-1 has been deleted. The structure of MFE1-DH shows that it consists o...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.10.085

    authors： Taskinen JP,Kiema TR,Hiltunen JK,Wierenga RK

    更新日期：2006-01-27 00:00:00

  • Ordered multi-site phosphorylation of the splicing factor ASF/SF2 by SRPK1.

    abstract：:The human alternative splicing factor ASF/SF2, an SR (serine-arginine-rich) protein involved in mRNA splicing control, is activated by the multisite phosphorylation of its C-terminal RS domain, a segment containing numerous arginine-serine dipeptide repeats. The protein kinase responsible for this modification, SR-spe...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.08.029

    authors： Ma CT,Velazquez-Dones A,Hagopian JC,Ghosh G,Fu XD,Adams JA

    更新日期：2008-02-08 00:00:00

  • Structural plasticity of the nuclear pore complex.

    abstract：:The nuclear pore complex (NPC) is strategically located at continuous junctions of the inner and outer nuclear membranes to catalyze macromolecular transport, without impending the diffusion of small molecules. In this paper, the structural plasticity of 4412 NPCs in isolated nuclear envelopes has been evaluated, util...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(95)80050-6

    authors： Akey CW

    更新日期：1995-04-28 00:00:00

  • Insights into RNA virus mutant spectrum and lethal mutagenesis events: replicative interference and complementation by multiple point mutants.

    abstract：:RNA virus behavior can be influenced by interactions among viral genomes and their expression products within the mutant spectra of replicating viral quasispecies. Here, we report the extent of interference of specific capsid and polymerase mutants of foot-and-mouth disease virus (FMDV) on replication of wild-type (wt...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.03.074

    authors： Perales C,Mateo R,Mateu MG,Domingo E

    更新日期：2007-06-15 00:00:00

  • Crystallization and initial crystallographic results for pepstatin A inhibited bovine cathepsin D.

    abstract：:Cathepsin D was purified from bovine liver by a method using two pepstatin A affinity columns. The eluted protein was combined with pepstatin A and the complex crystallized from 15% polyethylene glycol 8000 at pH 5.9. The crystals diffract to a resolution of 3.0 A and have space group P2(1)2(1)2(1) with unit cell dime...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90539-v

    authors： Bieber F,Brachvogel V,Arni R,Fusek M,Metcalf P

    更新日期：1992-10-20 00:00:00

  • Oligomerization of the SPP1 scaffolding protein.

    abstract：:Viral scaffolding proteins direct polymerization of major capsid protein subunits into icosahedral procapsid structures. The scaffolding protein of bacteriophage SPP1 was engineered with a C-terminal hexahistidine tag (gp11-His(6)) and purified. The protein is an alpha-helical-rich molecule with a very elongated shape...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.02.028

    authors： Poh SL,el Khadali F,Berrier C,Lurz R,Melki R,Tavares P

    更新日期：2008-05-02 00:00:00

  • The 1.2 A structure of a novel quorum-sensing protein, Bacillus subtilis LuxS.

    abstract：:In bacteria, the regulation of gene expression in response to changes in cell density is called quorum sensing. The autoinducer-2 production protein LuxS, is involved in a novel quorum-sensing system and is thought to catalyse the degradation of S-ribosylhomocysteine to homocysteine and the autoinducer molecule 4,5-di...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5027

    authors： Ruzheinikov SN,Das SK,Sedelnikova SE,Hartley A,Foster SJ,Horsburgh MJ,Cox AG,McCleod CW,Mekhalfia A,Blackburn GM,Rice DW,Baker PJ

    更新日期：2001-10-12 00:00:00

  • NMR characterization of full-length farnesylated and non-farnesylated H-Ras and its implications for Raf activation.

    abstract：:The C terminus, also known as the hypervariable region (residues 166-189), of H-, N-, and K-Ras proteins has sequence determinants necessary for full activation of downstream effectors such as Raf kinase and PI-3 kinase as well as for the correct targeting of Ras proteins to lipid rafts and non-raft membranes. There i...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.08.106

    authors： Thapar R,Williams JG,Campbell SL

    更新日期：2004-11-05 00:00:00

  • Intrinsic disorder in cell-signaling and cancer-associated proteins.

    abstract：:The number of intrinsically disordered proteins known to be involved in cell-signaling and regulation is growing rapidly. To test for a generalized involvement of intrinsic disorder in signaling and cancer, we applied a neural network predictor of natural disordered regions (PONDR VL-XT) to four protein datasets: huma...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00969-5

    authors： Iakoucheva LM,Brown CJ,Lawson JD,Obradović Z,Dunker AK

    更新日期：2002-10-25 00:00:00

  • A specific hydrophobic core in the alpha-lactalbumin molten globule.

    abstract：:Molten globules are partially structured protein folding intermediates that adopt a native-like overall backbone topology in the absence of extensive detectable tertiary interactions. It is important to determine the extent of specific tertiary structure present in molten globules and to understand the role of specifi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1825

    authors： Wu LC,Kim PS

    更新日期：1998-07-03 00:00:00

  • Differential expression of two clusters of mouse histone genes.

    abstract：:The mouse histone mRNAs coded for by three different cloned DNA fragments have been characterized. Two of these cloned DNA fragments, MM221 and MM291, located on chromosome 13, code for H3, H2b and H2a histone mRNAs, which are expressed at low levels in cultured mouse cells and fetal mice. The other DNA fragment, MM61...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(85)90211-6

    authors： Graves RA,Wellman SE,Chiu IM,Marzluff WF

    更新日期：1985-05-25 00:00:00

  • Solution Conformation of Bovine Leukemia Virus Gag Suggests an Elongated Structure.

    abstract：:Bovine leukemia virus (BLV) is a deltaretrovirus that infects domestic cattle. The structural protein Gag, found in all retroviruses, is a polyprotein comprising three major functional domains: matrix (MA), capsid (CA), and nucleocapsid (NC). Previous studies have shown that both mature BLV MA and NC are able to bind ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.01.036

    authors： Qualley DF,Cooper SE,Ross JL,Olson ED,Cantara WA,Musier-Forsyth K

    更新日期：2019-03-15 00:00:00

  • Mutations improving the folding of phage P22 tailspike protein affect its receptor binding activity.

    abstract：:Four previously isolated mutations in Salmonella phage P22 tailspike protein were used to study the relationship between protein stability, folding, and function. Tailspike protein binds and hydrolyzes the repetitive O-antigen structure in Salmonella lipopolysaccharide. Four mutations (V331G, V331A, A334V, A334I) are ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3165

    authors： Baxa U,Steinbacher S,Weintraub A,Huber R,Seckler R

    更新日期：1999-10-29 00:00:00

  • Genome-wide survey of prokaryotic O-protein phosphatases.

    abstract：:Complex and diverse signal transduction circuits are responsible for the efficient functioning of cellular network. Protein kinases and O-protein phosphatases are primarily responsible for propagating such stimuli within a eukaryotic cell. However, there is limited understanding of O-protein phosphatases in the prokar...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.07.004

    authors： Bhaduri A,Sowdhamini R

    更新日期：2005-09-23 00:00:00

  • Insulin assembly damps conformational fluctuations: Raman analysis of amide I linewidths in native states and fibrils.

    abstract：:The crystal structure of insulin has been investigated in a variety of dimeric and hexameric assemblies. Interest in dynamics has been stimulated by conformational variability among crystal forms and evidence suggesting that the functional monomer undergoes a conformational change on receptor binding. Here, we employ ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00536-9

    authors： Dong J,Wan Z,Popov M,Carey PR,Weiss MA

    更新日期：2003-07-04 00:00:00

  • Shape-specific nucleotide binding of single-stranded RNA by the GLD-1 STAR domain.

    abstract：:Proteins containing the STAR RNA-binding domain fulfill vital roles in RNA biogenesis, yet a detailed understanding of STAR domain RNA binding specificity is lacking. In Caenorhabditis elegans, the STAR protein GLD-1 directly binds the 28 nucleotide recognition element TGE within the 3' untranslated region of tra-2 mR...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.11.049

    authors： Lehmann-Blount KA,Williamson JR

    更新日期：2005-02-11 00:00:00

  • Structures of dimeric nonstandard nucleotide triphosphate pyrophosphatase from Pyrococcus horikoshii OT3: functional significance of interprotomer conformational changes.

    abstract：:Nonstandard nucleotide triphosphate pyrophosphatase (NTPase) can efficiently hydrolyze nonstandard purine nucleotides in the presence of divalent cations. The crystal structures of the NTPase from Pyrococcus horikoshii OT3 (PhNTPase) have been determined in two unliganded forms and in three liganded forms with inosine...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.11.018

    authors： Lokanath NK,Pampa KJ,Takio K,Kunishima N

    更新日期：2008-01-25 00:00:00

  • Specificity of antitermination mechanisms. Suppression of the terminator cluster T1-T2 of Escherichia coli ribosomal RNA operon, rrnB, by phage lambda antiterminators.

    abstract：:Transcription of the ribosomal RNA operons (rrn) in Escherichia coli is subject to an antitermination mechanism whereby RNA polymerase is modified to a termination-resistant form during transit through the rrn leader region. This antitermination mechanism is unable to overcome the T1-T2 terminator cluster located at t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90737-q

    authors： Ghosh B,Grzadzielska E,Bhattacharya P,Peralta E,DeVito J,Das A

    更新日期：1991-11-05 00:00:00