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Specificity of antitermination mechanisms. Suppression of the terminator cluster T1-T2 of Escherichia coli ribosomal RNA operon, rrnB, by phage lambda antiterminators.

Abstract：

:Transcription of the ribosomal RNA operons (rrn) in Escherichia coli is subject to an antitermination mechanism whereby RNA polymerase is modified to a termination-resistant form during transit through the rrn leader region. This antitermination mechanism is unable to overcome the T1-T2 terminator cluster located at the end of an rrn operon, such as rrnB. We have tested the specificity with which the T1-T2 terminators override an antitermination mechanism, by placing the terminator cluster downstream from the nut and qut sites recognized by phage lambda N and Q gene antiterminators, respectively. Measurement of downstream gene expression shows that RNA polymerase modified by either N or Q reads through the T1-T2 terminators quite efficiently. This supports the view that T1-T2 are not superterminators, and that the rrn antitermination mechanism may have a restricted terminator specificity.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Ghosh B,Grzadzielska E,Bhattacharya P,Peralta E,DeVito J,Das A

doi

10.1016/0022-2836(91)90737-q

subject

Has Abstract

pub_date

1991-11-05 00:00:00

pages

59-66

issue

1

eissn

0022-2836

issn

1089-8638

pii

0022-2836(91)90737-Q

journal_volume

222

pub_type

杂志文章

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